################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BbrPI BbsI BbvCI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspEI BspLU11I BsrDI BsrGI Bst1107I BstAPI BstBI BstZ17I CelII CpoI DraI DraII DraIII DrdI Eam1105I EcoNI EcoO109I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI MamI MfeI MluI MroI MunI NdeI NheI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I RsrII SacI SalI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp509I Tth111I Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AocI 756 CCTGAGG CC^T[ACGT]AGG Aor51HI 1193 AGCGCT AGC^GCT ApaI 1410 GGGCCC GGGCC^C AscI 1300 GGCGCGCC GG^CGCGCC AspHI 319 GTGCTC G[AT]GC[AT]^C AviII 1079 TGCGCA TGC^GCA BamHI 761 GGATCC G^GATCC BcgI 890 GCATTGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 456 GTATCC GTATCC(6/5) BseRI 618 CTCCTC GAGGAG(10/8) BsgI 384 CTGCAC GTGCAG(16/14) BsiHKAI 319 GTGCTC G[AT]GC[AT]^C BsmFI 1456 GGGAC GGGAC(10/14) BspDI 421 ATCGAT AT^CGAT BspHI 722 TCATGA T^CATGA BssSI 781 CACGAG CACGAG(-5/-1) BstXI 134 CCATCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 756 CCTGAGG CC^T[ACGT]AGG ClaI 421 ATCGAT AT^CGAT Eco47III 1193 AGCGCT AGC^GCT Eco81I 756 CCTGAGG CC^T[ACGT]AGG FspI 1079 TGCGCA TGC^GCA HincII 253 GTCAAC GT[CT]^[GA]AC HindII 253 GTCAAC GT[CT]^[GA]AC MseI 851 TTAA T^TAA NaeI 558 GCCGGC GCC^GGC NcoI 263 CCATGG C^CATGG NgoMIV 558 GCCGGC G^CCGGC NspI 1535 ACATGC [GA]CATG^[CT] PaeR7I 93 CTCGAG C^TCGAG PstI 451 CTGCAG CTGCA^G PvuI 423 CGATCG CGAT^CG RcaI 722 TCATGA T^CATGA SapI 1416 GCTCTTC GCTCTTC(1/4) SfaNI 1082 GCATC GCATC(5/9) SfcI 451 CTGCAG C^T[GA][CT]AG SmlI 93 CTCGAG C^T[CT][GA]AG TfiI 3 GAATC G^A[AT]TC Tru9I 851 TTAA T^TAA XhoI 93 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 666 GTAC GT^AC 800 GTAC AlwNI 35 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG 501 CAGTGTCTG AvaII 1256 GGACC G^G[AT]CC 1457 GGACC BsrBI 1414 CCGCTC CCGCTC(-3/-3) 1242 GAGCGG BssHII 1068 GCGCGC G^CGCGC 1301 GCGCGC BstEII 468 GGTCACC G^GT[ACGT]ACC 588 GGTCACC BstPI 468 GGTCACC G^GT[ACGT]ACC 588 GGTCACC BstYI 344 GGATCT [GA]^GATC[CT] 761 GGATCC EarI 613 CTCTTC CTCTTC(1/4) 1417 CTCTTC EcoO65I 468 GGTCACC G^GT[ACGT]ACC 588 GGTCACC Ksp632I 613 CTCTTC CTCTTC(1/4) 1417 CTCTTC MaeII 883 ACGT A^CGT 1502 ACGT MflI 344 GGATCT [GA]^GATC[CT] 761 GGATCC MslI 135 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 432 CATCGGGGTG RsaI 666 GTAC GT^AC 800 GTAC SfiI 1387 GGCCGCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1497 GGCCGACGTGGCC XhoII 344 GGATCT [GA]^GATC[CT] 761 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AvaI BalI BanI BanII BbeI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaJI BsiEI BsiYI BslI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EagI EclXI Eco52I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeI MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NotI PleI PvuII SacII Sau3AI Sau96I ScrFI SfoI SmaI StyI TaqI TseI Tsp45I TspRI TthHB8I XmaCI XmaI