(Circular) (Six-base) MAPSORT of: pFN21AB7037.seq Check: 6816 from: 1 to: 6265 pFN21AB7037 6265 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2053 2238 2053 Size: 185 6080 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3608 1937 Size: 1671 4594 Acc65I G'GTAC_C Cuts at: 3586 3586 Size: 6265 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2993 4328 5712 5980 1632 Size: 1361 1335 1384 268 1917 Fragments arranged by size: 1917 1384 1361 1335 268 AfeI AGC'GCT Cuts at: 3200 3200 Size: 6265 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 5164 6141 1203 Size: 3961 977 1327 Fragments arranged by size: 3961 1327 977 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 4994 4994 Size: 6265 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6265 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6265 AlwNI CAG_nnn'CTG Cuts at: 2045 2511 4008 5580 2045 Size: 466 1497 1572 2730 Fragments arranged by size: 2730 1572 1497 466 ApaI G_GGCC'C Cuts at: 3419 3419 Size: 6265 ApaLI G'TGCA_C Cuts at: 4322 5478 4322 Size: 1156 5109 ApoI r'AATT_y Cuts at: 1493 3574 3800 3952 6061 1493 Size: 2081 226 152 2109 1697 Fragments arranged by size: 2109 2081 1697 226 152 AscI GG'CGCG_CC Cuts at: 3306 3306 Size: 6265 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 2098 3092 3286 3338 3412 3449 3458 Size: 73 155 994 194 52 74 37 9 Cuts at: 3458 3554 3590 1870 Size: 96 36 4545 Fragments arranged by size: 4545 994 194 155 96 74 73 52 37 36 9 BamHI G'GATC_C Cuts at: 2766 3595 3962 2766 Size: 829 367 5069 Fragments arranged by size: 5069 829 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2162 2226 2566 3096 3349 Size: 325 217 627 375 64 340 530 253 Cuts at: 3349 3356 3473 3550 3586 5041 618 Size: 7 117 77 36 1455 1842 Fragments arranged by size: 1842 1455 627 530 375 340 325 253 217 117 77 64 36 7 BanII G_rGCy'C Cuts at: 1391 2636 3287 3387 3419 3450 3458 3584 1391 Size: 1245 651 100 32 31 8 126 4072 Fragments arranged by size: 4072 1245 651 126 100 32 31 8 BbeI G_GCGC'C Cuts at: 2166 3353 3360 3477 3554 2166 Size: 1187 7 117 77 4877 Fragments arranged by size: 4877 1187 117 77 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5294 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2884 2918 4456 4490 1903 Size: 34 947 34 1538 34 3678 Fragments arranged by size: 3678 1538 947 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2472 4157 5367 2472 Size: 1685 1210 3370 Fragments arranged by size: 3370 1685 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6265 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2495 3150 3249 Size: 107 122 71 631 605 823 655 99 Cuts at: 3249 3399 3509 4876 136 Size: 150 110 1367 1525 Fragments arranged by size: 1525 1367 823 655 631 605 150 122 110 107 99 71 BglII A'GATC_T Cuts at: 6260 6260 Size: 6265 BlpI GC'TnA_GC Cuts at: 3682 3682 Size: 6265 Bme1580I G_kGCm'C Cuts at: 1790 3419 4326 5482 1790 Size: 1629 907 1156 2573 Fragments arranged by size: 2573 1629 1156 907 BmgBI CAC'GTC Cuts at: 1318 3508 1318 Size: 2190 4075 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2062 2344 3996 Size: 416 53 83 399 24 645 282 1652 Cuts at: 3996 4954 442 Size: 958 1753 Fragments arranged by size: 1753 1652 958 645 416 399 282 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2848 3040 3215 4925 1239 Size: 1609 192 175 1710 2579 Fragments arranged by size: 2579 1710 1609 192 175 Bpu10I CC'TnA_GC Cuts at: 2798 3245 3365 3381 2798 Size: 447 120 16 5682 Fragments arranged by size: 5682 447 120 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4390 5255 5553 4390 Size: 865 298 5102 Fragments arranged by size: 5102 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4928 882 Size: 617 3429 2219 Fragments arranged by size: 3429 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4056 493 Size: 711 2852 2702 Fragments arranged by size: 2852 2702 711 BsaBI GATnn'nnATC Cuts at: 6259 6259 Size: 6265 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2163 2237 2985 3127 Size: 53 83 186 936 630 74 748 142 Cuts at: 3127 3350 3357 3474 3551 4072 4454 5089 275 Size: 223 7 117 77 521 382 635 1451 Fragments arranged by size: 1451 936 748 635 630 521 382 223 186 142 117 83 77 74 53 7 BsaWI w'CCGG_w Cuts at: 4698 5096 5370 5517 5928 4698 Size: 398 274 147 411 5035 Fragments arranged by size: 5035 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3038 3068 1178 Size: 30 165 30 1635 30 4375 Fragments arranged by size: 4375 1635 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2614 1186 Size: 1428 4837 BseYI C'CCAG_C Cuts at: 2118 2158 2341 3010 5468 5964 2118 Size: 40 183 669 2458 496 2419 Fragments arranged by size: 2458 2419 669 496 183 40 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2374 1163 Size: 573 638 5054 Fragments arranged by size: 5054 638 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2431 3112 3118 3173 3394 3403 Size: 275 186 430 681 6 55 221 9 Cuts at: 3403 3504 3715 4476 5504 1540 Size: 101 211 761 1028 2301 Fragments arranged by size: 2301 1028 761 681 430 275 221 211 186 101 55 9 6 BsiHKAI G_wGCw'C Cuts at: 2328 4326 4411 5482 2328 Size: 1998 85 1071 3111 Fragments arranged by size: 3111 1998 1071 85 BsmI GAATG_Cn' Cuts at: 3776 3869 3776 Size: 93 6172 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2328 2636 3287 3387 3419 3450 3458 Size: 399 538 308 651 100 32 31 8 Cuts at: 3458 3584 4326 4411 5482 1391 Size: 126 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 651 538 399 308 126 100 85 32 31 8 BspEI T'CCGG_A Cuts at: 5928 5928 Size: 6265 BspHI T'CATG_A Cuts at: 2727 4154 5884 2727 Size: 1427 1730 3108 Fragments arranged by size: 3108 1730 1427 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2053 2238 2286 3620 844 Size: 1209 185 48 1334 3489 Fragments arranged by size: 3489 1334 1209 185 48 BsrBI CCG'CTC Cuts at: 1394 1823 3249 3421 4152 1394 Size: 429 1426 172 731 3507 Fragments arranged by size: 3507 1426 731 429 172 BsrDI GCAATG_nn' Cuts at: 65 1248 4760 4934 65 Size: 1183 3512 174 1396 Fragments arranged by size: 3512 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2024 2223 2563 3168 4909 5096 1700 Size: 110 214 199 340 605 1741 187 2869 Fragments arranged by size: 2869 1741 605 340 214 199 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6265 BssHII G'CGCG_C Cuts at: 1925 3073 3306 1925 Size: 1148 233 4884 Fragments arranged by size: 4884 1148 233 BssSI C'ACGA_G Cuts at: 2786 4325 5337 2786 Size: 1539 1012 3714 Fragments arranged by size: 3714 1539 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6265 BstBI TT'CG_AA Cuts at: 5069 5069 Size: 6265 BstEII G'GTnAC_C Cuts at: 1184 2473 2593 1184 Size: 1289 120 4856 Fragments arranged by size: 4856 1289 120 BstXI CCAn_nnnn'nTGG Cuts at: 2146 2146 Size: 6265 BstYI r'GATC_y Cuts at: 1972 2349 2766 3595 3962 4347 4364 5816 5902 Size: 377 417 829 367 385 17 1452 86 Cuts at: 5902 5914 6260 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 829 417 385 377 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2762 2762 Size: 6265 BtgI C'CryG_G Cuts at: 513 1065 2268 2373 3106 3112 3173 3301 3397 Size: 552 1203 105 733 6 61 128 96 Cuts at: 3397 3498 513 Size: 101 3280 Fragments arranged by size: 3280 1203 733 552 128 105 101 96 61 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2923 487 Size: 683 56 1697 3829 Fragments arranged by size: 3829 1697 683 56 BtsI GCAGTG_nn' Cuts at: 2111 3781 4575 4595 2111 Size: 1670 794 20 3781 Fragments arranged by size: 3781 1670 794 20 ClaI AT'CG_AT Cuts at: 1554 2427 1554 Size: 873 5392 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6230 DraI TTT'AAA Cuts at: 3569 3569 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5272 809 Size: 507 3956 1802 Fragments arranged by size: 3956 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2026 2265 2271 Size: 54 1425 50 275 18 196 239 6 Cuts at: 2271 2691 3109 3115 3170 3391 3400 3501 3510 Size: 420 418 6 55 221 9 101 9 Cuts at: 3510 3712 4601 6230 8 Size: 202 889 1629 43 Fragments arranged by size: 1629 1425 889 420 418 275 239 221 202 196 101 55 54 50 43 18 9 9 6 6 EagI C'GGCC_G Cuts at: 1537 1812 3109 3115 3170 3391 3400 3501 3712 Size: 275 1297 6 55 221 9 101 211 Cuts at: 3712 1537 Size: 4090 Fragments arranged by size: 4090 1297 275 221 211 101 55 9 6 EarI CTCTTCn'nnn_ Cuts at: 1353 2624 3428 4195 1353 Size: 1271 804 767 3423 Fragments arranged by size: 3423 1271 804 767 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2362 4852 5226 5372 387 Size: 1064 138 773 2490 374 146 1280 Fragments arranged by size: 2490 1280 1064 773 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2848 2993 3040 3215 4328 4925 5712 Size: 393 1216 145 47 175 1113 597 787 Cuts at: 5712 5980 1239 Size: 268 1524 Fragments arranged by size: 1524 1216 1113 787 597 393 268 175 145 47 EcoRI G'AATT_C Cuts at: 3574 3952 3574 Size: 378 5887 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2509 2509 Size: 6265 FspI TGC'GCA Cuts at: 3086 4771 3086 Size: 1685 4580 FspAI rTGC'GCAy Cuts at: 3086 3086 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 2166 2501 3202 3353 3360 3477 3554 5412 Size: 488 335 701 151 7 117 77 1858 Cuts at: 5412 1678 Size: 2531 Fragments arranged by size: 2531 1858 701 488 335 151 117 77 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4906 4938 4980 5012 1373 Size: 32 135 32 3334 32 42 32 2626 Fragments arranged by size: 3334 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2260 3609 3855 669 Size: 988 281 322 1349 246 3079 Fragments arranged by size: 3079 1349 988 322 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6265 HpaI GTT'AAC Cuts at: 3855 3855 Size: 6265 KasI G'GCGC_C Cuts at: 2162 3349 3356 3473 3550 2162 Size: 1187 7 117 77 4877 Fragments arranged by size: 4877 1187 117 77 7 KpnI G_GTAC'C Cuts at: 3590 3590 Size: 6265 MfeI C'AATT_G Cuts at: 3864 3864 Size: 6265 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2766 5379 5563 1305 Size: 1461 2613 184 2007 Fragments arranged by size: 2613 2007 1461 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2267 2693 3512 10 Size: 54 1425 343 435 426 819 2763 Fragments arranged by size: 2763 1425 819 435 426 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2144 2441 4223 4582 4741 518 Size: 648 978 297 1782 359 159 2042 Fragments arranged by size: 2042 1782 978 648 359 297 159 MspA1I CmG'CkG Cuts at: 1606 2122 2312 3027 3108 3114 3234 3240 3259 Size: 516 190 715 81 6 120 6 19 Cuts at: 3259 3303 3399 3500 3681 4358 5506 5751 1606 Size: 44 96 101 181 677 1148 245 2120 Fragments arranged by size: 2120 1148 715 677 516 245 190 181 120 101 96 81 44 19 6 6 NaeI GCC'GGC Cuts at: 2565 2565 Size: 6265 NarI GG'CG_CC Cuts at: 2163 3350 3357 3474 3551 2163 Size: 1187 7 117 77 4877 Fragments arranged by size: 4877 1187 117 77 7 NcoI C'CATG_G Cuts at: 513 1065 2268 513 Size: 552 1203 4510 Fragments arranged by size: 4510 1203 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6265 NgoMIV G'CCGG_C Cuts at: 2563 2563 Size: 6265 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6265 NotI GC'GGCC_GC Cuts at: 3109 3115 3400 3712 3109 Size: 6 285 312 5662 Fragments arranged by size: 5662 312 285 6 NspI r_CATG'y Cuts at: 1132 1229 3544 3623 5168 1132 Size: 97 2315 79 1545 2229 Fragments arranged by size: 2315 2229 1545 97 79 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6265 PciI A'CATG_T Cuts at: 5164 5164 Size: 6265 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6265 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4334 4366 5847 5879 4334 Size: 32 1481 32 4720 Fragments arranged by size: 4720 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6265 PsiI TTA'TAA Cuts at: 3835 3835 Size: 6265 PspOMI G'GGCC_C Cuts at: 3415 3415 Size: 6265 PspXI vC'TCGA_Gb Cuts at: 2098 2098 Size: 6265 PstI C_TGCA'G Cuts at: 830 2460 3617 830 Size: 1630 1157 3478 Fragments arranged by size: 3478 1630 1157 PvuI CG_AT'CG Cuts at: 2001 2431 2001 Size: 430 5835 PvuII CAG'CTG Cuts at: 2122 3027 3240 2122 Size: 905 213 5147 Fragments arranged by size: 5147 905 213 SacII CC_GC'GG Cuts at: 3109 3115 3304 3400 3501 3109 Size: 6 189 96 101 5873 Fragments arranged by size: 5873 189 101 96 6 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SapI GCTCTTCn'nnn_ Cuts at: 3428 3428 Size: 6265 SbfI CC_TGCA'GG Cuts at: 3617 3617 Size: 6265 ScaI AGT'ACT Cuts at: 4513 6225 4513 Size: 1712 4553 SfcI C'TryA_G Cuts at: 826 1045 2456 3613 4748 5429 5620 826 Size: 219 1411 1157 1135 681 191 1471 Fragments arranged by size: 1471 1411 1157 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3399 3509 3399 Size: 110 6155 SfoI GGC'GCC Cuts at: 2164 3351 3358 3475 3552 2164 Size: 1187 7 117 77 4877 Fragments arranged by size: 4877 1187 117 77 7 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 3288 3460 3556 3592 1872 Size: 1416 172 96 36 4545 Fragments arranged by size: 4545 1416 172 96 36 SmlI C'TyrA_G Cuts at: 820 1017 1943 2098 4369 5270 5532 820 Size: 197 926 155 2271 901 262 1553 Fragments arranged by size: 2271 1553 926 901 262 197 155 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 3623 1132 Size: 2491 3774 SrfI GCCC'GGGC Cuts at: 3288 3556 3288 Size: 268 5997 SspI AAT'ATT Cuts at: 5 52 4189 5 Size: 47 4137 2081 Fragments arranged by size: 4137 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6265 StyI C'CwwG_G Cuts at: 513 1065 2268 2548 2748 2808 2814 3704 513 Size: 552 1203 280 200 60 6 890 3074 Fragments arranged by size: 3074 1203 890 552 280 200 60 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2326 2541 2584 3458 3479 4292 4309 Size: 1255 746 215 43 874 21 813 17 Cuts at: 4309 4462 4647 5092 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 874 813 746 445 215 185 153 43 21 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2670 4511 6223 Size: 274 80 33 51 1443 693 1841 1712 Cuts at: 6223 96 Size: 138 Fragments arranged by size: 1841 1712 1443 693 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2341 2369 2373 2401 6225 6257 2341 Size: 28 4 28 3824 32 2349 Fragments arranged by size: 3824 2349 32 28 28 4 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6265 XbaI T'CTAG_A Cuts at: 3601 3601 Size: 6265 XhoI C'TCGA_G Cuts at: 1943 2098 1943 Size: 155 6110 XmaI C'CCGG_G Cuts at: 1870 3286 3458 3554 3590 1870 Size: 1416 172 96 36 4545 Fragments arranged by size: 4545 1416 172 96 36 XmnI GAAnn'nnTTC Cuts at: 4394 4394 Size: 6265 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI NruI NsiI PacI PfoI PmlI PpuMI PsrI RsrII SacI SanDI SexAI SwaI XcmI