(Circular) (Six-base) MAPSORT of: pFN21AE1812.seq Check: 8166 from: 1 to: 5689 pFN21AE1812 5689 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2435 2746 2435 Size: 311 5378 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3032 1937 Size: 1095 4594 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2102 2184 2753 3752 5136 5404 1632 Size: 470 82 569 999 1384 268 1917 Fragments arranged by size: 1917 1384 999 569 470 268 82 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 4588 5565 1203 Size: 3385 977 1327 Fragments arranged by size: 3385 1327 977 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5689 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2942 1184 Size: 1758 3931 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2274 2306 2274 Size: 32 5657 AlwNI CAG_nnn'CTG Cuts at: 2598 2609 3432 5004 2598 Size: 11 823 1572 3283 Fragments arranged by size: 3283 1572 823 11 ApaI G_GGCC'C Cuts at: 2935 2935 Size: 5689 ApaLI G'TGCA_C Cuts at: 3746 4902 3746 Size: 1156 4533 ApoI r'AATT_y Cuts at: 1493 2334 2746 2998 3224 3376 5485 1493 Size: 841 412 252 226 152 2109 1697 Fragments arranged by size: 2109 1697 841 412 252 226 152 AseI AT'TA_AT Cuts at: 160 1032 4243 160 Size: 872 3211 1606 Fragments arranged by size: 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 3014 1870 Size: 73 1071 4545 Fragments arranged by size: 4545 1071 73 BamHI G'GATC_C Cuts at: 3019 3386 3019 Size: 367 5322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3010 4465 618 Size: 325 217 627 1223 1455 1842 Fragments arranged by size: 1842 1455 1223 627 325 217 BanII G_rGCy'C Cuts at: 1391 2935 3008 1391 Size: 1544 73 4072 Fragments arranged by size: 4072 1544 73 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2863 928 Size: 971 964 3754 Fragments arranged by size: 3754 971 964 BbvCI CC'TCA_GC Cuts at: 2610 2887 2610 Size: 277 5412 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3880 3914 1903 Size: 34 1943 34 3678 Fragments arranged by size: 3678 1943 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2127 3581 4791 2127 Size: 1454 1210 3025 Fragments arranged by size: 3025 1454 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4300 136 Size: 107 122 71 631 605 2628 1525 Fragments arranged by size: 2628 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5684 5684 Size: 5689 BlpI GC'TnA_GC Cuts at: 3106 3106 Size: 5689 Bme1580I G_kGCm'C Cuts at: 1790 2935 2982 3750 4906 1790 Size: 1145 47 768 1156 2573 Fragments arranged by size: 2573 1156 1145 768 47 BmgBI CAC'GTC Cuts at: 1318 2511 1318 Size: 1193 4496 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3420 4378 442 Size: 416 53 83 399 24 2003 958 1753 Fragments arranged by size: 2003 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2151 2809 4349 1239 Size: 912 658 1540 2579 Fragments arranged by size: 2579 1540 912 658 Bpu10I CC'TnA_GC Cuts at: 2610 2887 2610 Size: 277 5412 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2642 3814 4679 4977 2642 Size: 1172 865 298 3354 Fragments arranged by size: 3354 1172 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4352 882 Size: 617 2853 2219 Fragments arranged by size: 2853 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3480 493 Size: 711 2276 2702 Fragments arranged by size: 2702 2276 711 BsaBI GATnn'nnATC Cuts at: 2115 5683 2115 Size: 3568 2121 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3496 3878 4513 275 Size: 53 83 186 936 1963 382 635 1451 Fragments arranged by size: 1963 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2119 4122 4520 4794 4941 5352 2119 Size: 2003 398 274 147 411 2456 Fragments arranged by size: 2456 2003 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2124 2154 2274 2304 2552 Size: 30 165 30 721 30 120 30 248 Cuts at: 2552 2582 1178 Size: 30 4285 Fragments arranged by size: 4285 721 248 165 120 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2627 1186 Size: 1441 4248 BseYI C'CCAG_C Cuts at: 2602 2769 4892 5388 2602 Size: 167 2123 496 2903 Fragments arranged by size: 2903 2123 496 167 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5116 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3139 3900 4928 1540 Size: 275 186 1138 761 1028 2301 Fragments arranged by size: 2301 1138 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3750 3835 4906 3750 Size: 85 1071 4533 Fragments arranged by size: 4533 1071 85 BsmI GAATG_Cn' Cuts at: 3200 3293 3200 Size: 93 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2935 2982 3008 3750 3835 4906 1391 Size: 399 1145 47 26 742 85 1071 2174 Fragments arranged by size: 2174 1145 1071 742 399 85 47 26 BspEI T'CCGG_A Cuts at: 2119 5352 2119 Size: 3233 2456 BspHI T'CATG_A Cuts at: 3578 5308 3578 Size: 1730 3959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2435 2746 3044 844 Size: 1591 311 298 3489 Fragments arranged by size: 3489 1591 311 298 BsrBI CCG'CTC Cuts at: 1394 1823 3576 1394 Size: 429 1753 3507 Fragments arranged by size: 3507 1753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4184 4358 65 Size: 1183 2936 174 1396 Fragments arranged by size: 2936 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4333 4520 1700 Size: 110 2523 187 2869 Fragments arranged by size: 2869 2523 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5689 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 2063 3749 4761 2063 Size: 1686 1012 2991 Fragments arranged by size: 2991 1686 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5689 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5689 BstYI r'GATC_y Cuts at: 1972 3019 3386 3771 3788 5240 5326 5338 5684 Size: 1047 367 385 17 1452 86 12 346 Cuts at: 5684 1972 Size: 1977 Fragments arranged by size: 1977 1452 1047 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2892 513 Size: 552 1827 3310 Fragments arranged by size: 3310 1827 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4950 Fragments arranged by size: 4950 683 56 BtsI GCAGTG_nn' Cuts at: 2206 2992 3205 3999 4019 2206 Size: 786 213 794 20 3876 Fragments arranged by size: 3876 794 786 213 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2355 2993 2355 Size: 638 5051 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4696 809 Size: 507 3380 1802 Fragments arranged by size: 3380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2260 3136 Size: 54 1425 50 275 18 237 193 876 Cuts at: 3136 4025 5654 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 876 275 237 193 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3136 1537 Size: 275 1324 4090 Fragments arranged by size: 4090 1324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2494 3619 1353 Size: 1141 1125 3423 Fragments arranged by size: 3423 1141 1125 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4276 4650 4796 387 Size: 1064 138 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2151 2184 2753 2809 3752 4349 Size: 393 470 49 33 569 56 943 597 Cuts at: 4349 5136 5404 1239 Size: 787 268 1524 Fragments arranged by size: 1524 943 787 597 569 470 393 268 56 49 33 EcoRI G'AATT_C Cuts at: 2746 2998 3376 2746 Size: 252 378 5059 Fragments arranged by size: 5059 378 252 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 4836 1678 Size: 3158 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2870 2902 4330 4362 4404 Size: 32 135 32 1298 32 1428 32 42 Cuts at: 4404 4436 1373 Size: 32 2626 Fragments arranged by size: 2626 1428 1298 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3033 3279 669 Size: 988 281 1095 246 3079 Fragments arranged by size: 3079 1095 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5689 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4803 4987 1305 Size: 3498 184 2007 Fragments arranged by size: 3498 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2069 2262 10 Size: 54 1425 343 237 193 3437 Fragments arranged by size: 3437 1425 343 237 193 54 MslI CAynn'nnrTG Cuts at: 518 1166 2924 3647 4006 4165 518 Size: 648 1758 723 359 159 2042 Fragments arranged by size: 2042 1758 723 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2606 2731 2769 3105 3782 4930 5175 1606 Size: 1000 125 38 336 677 1148 245 2120 Fragments arranged by size: 2120 1148 1000 677 336 245 125 38 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5137 NdeI CA'TA_TG Cuts at: 387 2072 387 Size: 1685 4004 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 3047 4592 1132 Size: 97 1818 1545 2229 Fragments arranged by size: 2229 1818 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5689 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 2020 1108 Size: 912 4777 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2274 2306 3758 3790 5271 5303 2274 Size: 32 1452 32 1481 32 2660 Fragments arranged by size: 2660 1481 1452 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PspOMI G'GGCC_C Cuts at: 2931 2931 Size: 5689 PstI C_TGCA'G Cuts at: 830 2536 2987 3041 830 Size: 1706 451 54 3478 Fragments arranged by size: 3478 1706 451 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 PvuII CAG'CTG Cuts at: 2606 2731 2769 2606 Size: 125 38 5526 Fragments arranged by size: 5526 125 38 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SapI GCTCTTCn'nnn_ Cuts at: 2494 2494 Size: 5689 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 2452 2570 2794 3937 5649 2452 Size: 118 224 1143 1712 2492 Fragments arranged by size: 2492 1712 1143 224 118 SfcI C'TryA_G Cuts at: 826 1045 2532 2947 2983 3037 4172 4853 5044 Size: 219 1487 415 36 54 1135 681 191 Cuts at: 5044 826 Size: 1471 Fragments arranged by size: 1487 1471 1135 681 415 219 191 54 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 3016 1872 Size: 1144 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2657 3793 4694 4956 820 Size: 197 926 714 1136 901 262 1553 Fragments arranged by size: 1553 1136 926 901 714 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 3047 1132 Size: 1915 3774 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5689 StyI C'CwwG_G Cuts at: 513 1065 2187 2500 3128 513 Size: 552 1122 313 628 3074 Fragments arranged by size: 3074 1122 628 552 313 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2731 3716 3733 3886 4071 4516 325 Size: 1255 1151 985 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1151 985 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2265 2450 2568 Size: 274 80 33 51 1443 288 185 118 Cuts at: 2568 2792 3935 5647 96 Size: 224 1143 1712 138 Fragments arranged by size: 1712 1443 1143 288 274 224 185 138 118 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2124 2156 5649 5681 2124 Size: 32 3493 32 2132 Fragments arranged by size: 3493 2132 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2632 2632 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 3014 1870 Size: 1144 4545 XmnI GAAnn'nnTTC Cuts at: 3818 3818 Size: 5689 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI