Restriction map for
pFN21AE4304
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI AluI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BanI BbeI BbrPI BbsI
BbvCI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MslI MspA1I MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI TthHB8I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AocI 740 CCTCAGG CC^T[ACGT]AGG
AspHI 199 GTGCTC G[AT]GC[AT]^C
AspI 22 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 59 GGTCC G^G[AT]CC
BamHI 332 GGATCC G^GATCC
BanII 826 GAGCCC G[GA]GC[CT]^C
BcgI 605 GCATCTACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 584 CTGGAG CTGGAG(16/14)
BseRI 823 GAGGAG GAGGAG(10/8)
BsgI 407 CTGCAC GTGCAG(16/14)
BsiHKAI 199 GTGCTC G[AT]GC[AT]^C
BsmI 96 GAATGC GAATGC(1/-1)
BsrBI 80 GAGCGG CCGCTC(-3/-3)
BsrDI 895 GCAATG GCAATG(2/0)
BssSI 142 CTCGTG CACGAG(-5/-1)
BstEII 399 GGTCACC G^GT[ACGT]ACC
BstPI 399 GGTCACC G^GT[ACGT]ACC
Bsu36I 740 CCTCAGG CC^T[ACGT]AGG
CfoI 659 GCGC GCG^C
DraII 58 AGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 802 GAAGAG CTCTTC(1/4)
Eco81I 740 CCTCAGG CC^T[ACGT]AGG
EcoO109I 58 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 399 GGTCACC G^GT[ACGT]ACC
EcoRI 770 GAATTC G^AATTC
HaeII 658 GGCGCT [GA]GCGC^[CT]
HapII 330 CCGG C^CGG
HhaI 659 GCGC GCG^C
HinP1I 659 GCGC G^CGC
HpaII 330 CCGG C^CGG
HphI 401 TCACC GGTGA(8/7)
Ksp632I 802 GAAGAG CTCTTC(1/4)
MspI 330 CCGG C^CGG
NlaIII 469 CATG CATG^
PflFI 22 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 239 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 58 AGGTCCT [GA]G^G[AT]CC[CT]
Tth111I 22 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 239 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 556 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwNI 240 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
487 CAGACACTG
BfaI 304 CTAG C^TAG
557 CTAG
BsaBI 333 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
508 GATATAAATC
BsmAI 425 GAGAC GTCTC(1/5)
481 GAGAC
BsrI 245 ACTGG ACTGG(1/-1)
492 ACTGG
Cac8I 636 GCTGGC GC[ACGT]^[ACGT]GC
857 GCAGGC
Cfr13I 59 GGTCC G^G[ACGT]CC
472 GGCCC
EcoNI 440 CCTTTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
736 CCTCCCTCAGG
EcoT14I 395 CCAAGG C^C[AT][AT]GG
445 CCAAGG
MaeI 304 CTAG C^TAG
557 CTAG
MaeII 194 ACGT A^CGT
809 ACGT
MamI 333 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
508 GATATAAATC
PstI 418 CTGCAG CTGCA^G
434 CTGCAG
Sau96I 59 GGTCC G^G[ACGT]CC
472 GGCCC
SfaNI 450 GATGC GCATC(5/9)
605 GCATC
SfcI 418 CTGCAG C^T[GA][CT]AG
434 CTGCAG
StyI 395 CCAAGG C^C[AT][AT]GG
445 CCAAGG
Tsp45I 298 GTGAC ^GT[GC]AC
400 GTCAC
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
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AciI AcsI AlwI ApoI BalI
BbvI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstYI DdeI DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MvaI MwoI
NdeII NlaIV PleI Sau3AI ScrFI
Tru9I TseI Tsp509I TspRI XhoII