(Circular) (Six-base) MAPSORT of: pFN21AE4304.seq Check: 1221 from: 1 to: 5608 pFN21AE4304 5608 bp With 182 enzymes: SgfI * March 28, 2012 14:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3418 278 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2951 1937 Size: 1014 4594 Acc65I G'GTAC_C Cuts at: 2929 2929 Size: 5608 AclI AA'CG_TT Cuts at: 1563 3735 4108 1563 Size: 2172 373 3063 Fragments arranged by size: 3063 2172 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2399 3671 5055 5323 1632 Size: 767 1272 1384 268 1917 Fragments arranged by size: 1917 1384 1272 767 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5411 AflIII A'CryG_T Cuts at: 1203 4507 5484 1203 Size: 3304 977 1327 Fragments arranged by size: 3304 1327 977 AgeI A'CCGG_T Cuts at: 4439 4439 Size: 5608 AhdI GACnn_n'nnGTC Cuts at: 4337 4337 Size: 5608 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5608 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2219 1184 Size: 1035 4573 AlwNI CAG_nnn'CTG Cuts at: 2250 2497 3351 4923 2250 Size: 247 854 1572 2935 Fragments arranged by size: 2935 1572 854 247 ApaLI G'TGCA_C Cuts at: 3665 4821 3665 Size: 1156 4452 ApoI r'AATT_y Cuts at: 1493 2369 2710 2775 2917 3143 3295 5404 1493 Size: 876 341 65 142 226 152 2109 1697 Fragments arranged by size: 2109 1697 876 341 226 152 142 65 AseI AT'TA_AT Cuts at: 160 1032 4162 160 Size: 872 3130 1606 Fragments arranged by size: 3130 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 AvaI C'yCGr_G Cuts at: 1870 1943 2933 1870 Size: 73 990 4545 Fragments arranged by size: 4545 990 73 BamHI G'GATC_C Cuts at: 2337 2938 3305 2337 Size: 601 367 4640 Fragments arranged by size: 4640 601 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2929 4384 618 Size: 325 217 627 1142 1455 1842 Fragments arranged by size: 1842 1455 1142 627 325 217 BanII G_rGCy'C Cuts at: 1391 2835 2927 1391 Size: 1444 92 4072 Fragments arranged by size: 4072 1444 92 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4637 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2599 2633 3799 3833 1903 Size: 34 662 34 1166 34 3678 Fragments arranged by size: 3678 1166 662 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3500 4710 3500 Size: 1210 4398 BclI T'GATC_A Cuts at: 1548 1548 Size: 5608 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4219 136 Size: 107 122 71 631 605 2547 1525 Fragments arranged by size: 2547 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5603 5603 Size: 5608 BlpI GC'TnA_GC Cuts at: 3025 3025 Size: 5608 Bme1580I G_kGCm'C Cuts at: 1790 2154 3669 4825 1790 Size: 364 1515 1156 2573 Fragments arranged by size: 2573 1515 1156 364 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5608 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2507 3339 4297 Size: 416 53 83 399 24 1090 832 958 Cuts at: 4297 442 Size: 1753 Fragments arranged by size: 1753 1090 958 832 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5608 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2610 4268 1239 Size: 1371 1658 2579 Fragments arranged by size: 2579 1658 1371 Bpu10I CC'TnA_GC Cuts at: 2207 2207 Size: 5608 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3733 4598 4896 3733 Size: 865 298 4445 Fragments arranged by size: 4445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4271 882 Size: 617 2772 2219 Fragments arranged by size: 2772 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3399 493 Size: 711 2195 2702 Fragments arranged by size: 2702 2195 711 BsaBI GATnn'nnATC Cuts at: 2342 2517 5602 2342 Size: 175 3085 2348 Fragments arranged by size: 3085 2348 175 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3415 3797 4432 275 Size: 53 83 186 936 1882 382 635 1451 Fragments arranged by size: 1882 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4041 4439 4713 4860 5271 4041 Size: 398 274 147 411 4378 Fragments arranged by size: 4378 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5383 Fragments arranged by size: 5383 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2843 1186 Size: 1657 3951 BseYI C'CCAG_C Cuts at: 2128 2160 2479 2636 4811 5307 2128 Size: 32 319 157 2175 496 2429 Fragments arranged by size: 2429 2175 496 319 157 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2397 1163 Size: 573 661 4374 Fragments arranged by size: 4374 661 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3058 3819 4847 1540 Size: 275 186 1057 761 1028 2301 Fragments arranged by size: 2301 1057 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2208 3669 3754 4825 2208 Size: 1461 85 1071 2991 Fragments arranged by size: 2991 1461 1071 85 BsmI GAATG_Cn' Cuts at: 2107 3119 3212 2107 Size: 1012 93 4503 Fragments arranged by size: 4503 1012 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 2208 2835 2927 3669 3754 4825 Size: 399 364 54 627 92 742 85 1071 Cuts at: 4825 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 627 399 364 92 85 54 BspEI T'CCGG_A Cuts at: 5271 5271 Size: 5608 BspHI T'CATG_A Cuts at: 3497 5227 3497 Size: 1730 3878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2963 844 Size: 2119 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2087 3495 1394 Size: 429 264 1408 3507 Fragments arranged by size: 3507 1408 429 264 BsrDI GCAATG_nn' Cuts at: 65 1248 2907 4103 4277 65 Size: 1183 1659 1196 174 1396 Fragments arranged by size: 1659 1396 1196 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4252 4439 1700 Size: 110 2442 187 2869 Fragments arranged by size: 2869 2442 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5608 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5608 BssSI C'ACGA_G Cuts at: 2147 3668 4680 2147 Size: 1521 1012 3075 Fragments arranged by size: 3075 1521 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5608 BstBI TT'CG_AA Cuts at: 4412 4412 Size: 5608 BstEII G'GTnAC_C Cuts at: 1184 2404 1184 Size: 1220 4388 BstYI r'GATC_y Cuts at: 1972 2322 2337 2628 2938 3305 3690 3707 5159 Size: 350 15 291 310 367 385 17 1452 Cuts at: 5159 5245 5257 5603 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 350 346 310 291 86 17 15 12 Bsu36I CC'TnA_GG Cuts at: 2746 2746 Size: 5608 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5056 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4869 Fragments arranged by size: 4869 683 56 BtsI GCAGTG_nn' Cuts at: 2436 3124 3918 3938 2436 Size: 688 794 20 4106 Fragments arranged by size: 4106 794 688 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5608 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5573 DraI TTT'AAA Cuts at: 2912 2912 Size: 5608 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4615 809 Size: 507 3299 1802 Fragments arranged by size: 3299 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2541 2568 2730 Size: 54 1425 50 275 18 711 27 162 Cuts at: 2730 3055 3944 5573 8 Size: 325 889 1629 43 Fragments arranged by size: 1629 1425 889 711 325 275 162 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3055 1537 Size: 275 1243 4090 Fragments arranged by size: 4090 1243 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2802 3538 1353 Size: 1449 736 3423 Fragments arranged by size: 3423 1449 736 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4195 4569 4715 387 Size: 1064 138 2606 374 146 1280 Fragments arranged by size: 2606 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2399 2610 3671 4268 5055 5323 1239 Size: 393 767 211 1061 597 787 268 1524 Fragments arranged by size: 1524 1061 787 767 597 393 268 211 EcoNI CCTnn'n_nnAGG Cuts at: 2449 2745 2449 Size: 296 5312 EcoO109I rG'GnC_Cy Cuts at: 2064 2064 Size: 5608 EcoRI G'AATT_C Cuts at: 2775 2917 3295 2775 Size: 142 378 5088 Fragments arranged by size: 5088 378 142 FspI TGC'GCA Cuts at: 4114 4114 Size: 5608 HaeII r_GCGC'y Cuts at: 1678 2667 4755 1678 Size: 989 2088 2531 Fragments arranged by size: 2531 2088 989 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2076 2108 2583 2613 2615 Size: 32 135 32 504 32 475 30 2 Cuts at: 2615 2645 4249 4281 4323 4355 1373 Size: 30 1604 32 42 32 2626 Fragments arranged by size: 2626 1604 504 475 135 42 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 2952 3198 669 Size: 988 281 1014 246 3079 Fragments arranged by size: 3079 1014 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5608 HpaI GTT'AAC Cuts at: 3198 3198 Size: 5608 KpnI G_GTAC'C Cuts at: 2933 2933 Size: 5608 MfeI C'AATT_G Cuts at: 3207 3207 Size: 5608 MluI A'CGCG_T Cuts at: 5484 5484 Size: 5608 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4722 4906 1305 Size: 3417 184 2007 Fragments arranged by size: 3417 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2543 2570 2732 10 Size: 54 1425 343 711 27 162 2886 Fragments arranged by size: 2886 1425 711 343 162 54 27 MslI CAynn'nnrTG Cuts at: 518 1166 3566 3925 4084 518 Size: 648 2400 359 159 2042 Fragments arranged by size: 2400 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3024 3701 4849 5094 1606 Size: 1418 677 1148 245 2120 Fragments arranged by size: 2120 1418 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5056 NdeI CA'TA_TG Cuts at: 387 387 Size: 5608 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5608 NotI GC'GGCC_GC Cuts at: 3055 3055 Size: 5608 NspI r_CATG'y Cuts at: 1132 1229 2966 4511 1132 Size: 97 1737 1545 2229 Fragments arranged by size: 2229 1737 1545 97 PasI CC'CwG_GG Cuts at: 1348 2138 1348 Size: 790 4818 PciI A'CATG_T Cuts at: 4507 4507 Size: 5608 PflMI CCAn_nnn'nTGG Cuts at: 1108 2250 1108 Size: 1142 4466 PmeI GTTT'AAAC Cuts at: 2912 2912 Size: 5608 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3677 3709 5190 5222 3677 Size: 32 1481 32 4063 Fragments arranged by size: 4063 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2064 2064 Size: 5608 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5608 PsiI TTA'TAA Cuts at: 3178 3178 Size: 5608 PstI C_TGCA'G Cuts at: 830 2427 2443 2960 830 Size: 1597 16 517 3478 Fragments arranged by size: 3478 1597 517 16 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5608 SalI G'TCGA_C Cuts at: 1936 2950 1936 Size: 1014 4594 SbfI CC_TGCA'GG Cuts at: 2960 2960 Size: 5608 ScaI AGT'ACT Cuts at: 3856 5568 3856 Size: 1712 3896 SfcI C'TryA_G Cuts at: 826 1045 2423 2439 2956 4091 4772 4963 826 Size: 219 1378 16 517 1135 681 191 1471 Fragments arranged by size: 1471 1378 1135 681 517 219 191 16 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5608 SmaI CCC'GGG Cuts at: 1872 2935 1872 Size: 1063 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3712 4613 4875 820 Size: 197 926 1769 901 262 1553 Fragments arranged by size: 1769 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3399 493 Size: 2906 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5608 SphI G_CATG'C Cuts at: 1132 2966 1132 Size: 1834 3774 SspI AAT'ATT Cuts at: 5 52 3532 5 Size: 47 3480 2081 Fragments arranged by size: 3480 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5608 StyI C'CwwG_G Cuts at: 513 1065 2400 2450 3047 513 Size: 552 1335 50 597 3074 Fragments arranged by size: 3074 1335 597 552 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2084 3635 3652 3805 3990 4435 325 Size: 1255 504 1551 17 153 185 445 1498 Fragments arranged by size: 1551 1498 1255 504 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3854 5566 96 Size: 274 80 33 51 1443 1877 1712 138 Fragments arranged by size: 1877 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5568 5600 5568 Size: 32 5576 Tth111I GACn'n_nGTC Cuts at: 1316 2030 1316 Size: 714 4894 XbaI T'CTAG_A Cuts at: 2561 2944 2561 Size: 383 5225 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5608 XmaI C'CCGG_G Cuts at: 1870 2933 1870 Size: 1063 4545 XmnI GAAnn'nnTTC Cuts at: 3737 3737 Size: 5608 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3416 276 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI