################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccII AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaII AviII AvrII BalI BamHI BanI BbeI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaHI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FauI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MscI MunI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp509I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 723 GTCTAC GT^[AC][GT]AC AccIII 615 TCCGGA T^CCGGA AluI 492 AGCT AG^CT AlwI 774 GGATC GGATC(4/5) ApaI 661 GGGCCC GGGCC^C AspHI 976 GTGCTC G[AT]GC[AT]^C AspI 736 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 661 GGGCCC G[GA]GC[CT]^C BbvCI 832 CCTCAGC CCTCAGC(-5/-2) BclI 950 TGATCA T^GATCA BcnI 102 CCGGG CC^[GC]GG BfaI 1054 CTAG C^TAG BsaBI 948 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 134 GGTCTC GGTCTC(1/5) BsaJI 965 CCTTGG C^C[ACGT][ACGT]GG BseAI 615 TCCGGA T^CCGGA BseRI 830 CTCCTC GAGGAG(10/8) BsiHKAI 976 GTGCTC G[AT]GC[AT]^C BsmFI 262 GGGAC GGGAC(10/14) BspEI 615 TCCGGA T^CCGGA BsrDI 561 CATTGC GCAATG(2/0) BstYI 774 GGATCT [GA]^GATC[CT] DraII 660 AGGGCCC [GA]G^G[ACGT]CC[CT] DraIII 100 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 979 CTCTTC CTCTTC(1/4) EcoNI 612 CCTTCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 660 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoT14I 965 CCTTGG C^C[AT][AT]GG FbaI 950 TGATCA T^GATCA HaeII 198 GGCGCT [GA]GCGC^[CT] Ksp632I 979 CTCTTC CTCTTC(1/4) MaeI 1054 CTAG C^TAG MamI 948 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 774 GGATCT [GA]^GATC[CT] MroI 615 TCCGGA T^CCGGA MseI 298 TTAA T^TAA NciI 102 CCGGG CC^[GC]GG NlaIV 661 GGGCCC GG[ACGT]^[ACGT]CC NspI 152 GCATGT [GA]CATG^[CT] PflFI 736 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 764 CCAAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 57 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 674 CTGCAG CTGCA^G PvuII 491 CAGCTG CAG^CTG SapI 978 GCTCTTC GCTCTTC(1/4) SfaNI 844 GCATC GCATC(5/9) SmlI 231 CTCAAG C^T[CT][GA]AG StyI 965 CCTTGG C^C[AT][AT]GG Tru9I 298 TTAA T^TAA Tth111I 736 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 764 CCAAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 774 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 123 GTAC GT^AC 327 GTAC AflIII 584 ACACGT A^C[GA][CT]GT 794 ACGTGT AvaI 270 CCCGAG C^[CT]CG[GA]G 643 CCCGAG BbrPI 585 CACGTG CAC^GTG 793 CACGTG BsaAI 585 CACGTG [CT]AC^GT[GA] 793 CACGTG BsgI 494 CTGCAC GTGCAG(16/14) 1093 GTGCAG BsoBI 270 CCCGAG C^[CT]CG[GA]G 643 CCCGAG BstEII 97 GGTCACC G^GT[ACGT]ACC 225 GGTGACC BstPI 97 GGTCACC G^GT[ACGT]ACC 225 GGTGACC CfoI 199 GCGC GCG^C 381 GCGC Cfr13I 661 GGGCC G^G[ACGT]CC 662 GGCCC EcoO65I 97 GGTCACC G^GT[ACGT]ACC 225 GGTGACC HaeIII 662 GGCC GG^CC 1120 GGCC HhaI 199 GCGC GCG^C 381 GCGC HinP1I 199 GCGC G^CGC 381 GCGC MslI 339 CATAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 909 CATGTCAATG MspA1I 318 CAGCGG C[AC]G^C[GT]G 491 CAGCTG PmaCI 585 CACGTG CAC^GTG 793 CACGTG PmlI 585 CACGTG CAC^GTG 793 CACGTG RsaI 123 GTAC GT^AC 327 GTAC Sau96I 661 GGGCC G^G[ACGT]CC 662 GGCCC SfcI 391 CTACAG C^T[GA][CT]AG 674 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AlwNI BbsI BbvI BmyI BpmI BpuAI BsaWI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI DdeI DpnII EcoRII Fnu4HI FokI HapII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspI MvaI NdeII NlaIII PleI Sau3AI ScrFI TfiI TseI Tsp45I TspRI