(Circular) (Six-base) MAPSORT of: pFN21AE2451.seq Check: 1100 from: 1 to: 5839 pFN21AE2451 5839 bp With 182 enzymes: SgfI * February 5, 2013 11:12 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3649 278 Size: 53 83 186 936 2113 2468 Fragments arranged by size: 2468 2113 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2729 3182 1937 Size: 792 453 4594 Fragments arranged by size: 4594 792 453 Acc65I G'GTAC_C Cuts at: 3160 3160 Size: 5839 AclI AA'CG_TT Cuts at: 1563 3966 4339 1563 Size: 2403 373 3063 Fragments arranged by size: 3063 2403 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2058 2246 3902 5286 5554 1632 Size: 426 188 1656 1384 268 1917 Fragments arranged by size: 1917 1656 1384 426 268 188 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5642 AflIII A'CryG_T Cuts at: 1203 2589 2799 4738 5715 1203 Size: 1386 210 1939 977 1327 Fragments arranged by size: 1939 1386 1327 977 210 AgeI A'CCGG_T Cuts at: 4670 4670 Size: 5839 AhdI GACnn_n'nnGTC Cuts at: 4568 4568 Size: 5839 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5839 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2366 2608 1184 Size: 1182 242 4415 Fragments arranged by size: 4415 1182 242 AlwNI CAG_nnn'CTG Cuts at: 2081 2693 2867 3582 5154 2081 Size: 612 174 715 1572 2766 Fragments arranged by size: 2766 1572 715 612 174 ApaI G_GGCC'C Cuts at: 2670 2670 Size: 5839 ApaLI G'TGCA_C Cuts at: 3896 5052 3896 Size: 1156 4683 ApoI r'AATT_y Cuts at: 1493 3148 3374 3526 5635 1493 Size: 1655 226 152 2109 1697 Fragments arranged by size: 2109 1697 1655 226 152 AseI AT'TA_AT Cuts at: 160 1032 4393 160 Size: 872 3361 1606 Fragments arranged by size: 3361 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 AvaI C'yCGr_G Cuts at: 1870 1943 2275 2648 3164 1870 Size: 73 332 373 516 4545 Fragments arranged by size: 4545 516 373 332 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2847 2880 2847 Size: 33 5806 BamHI G'GATC_C Cuts at: 3169 3536 3169 Size: 367 5472 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3160 4615 618 Size: 325 217 627 1373 1455 1842 Fragments arranged by size: 1842 1455 1373 627 325 217 BanII G_rGCy'C Cuts at: 1391 2670 3158 1391 Size: 1279 488 4072 Fragments arranged by size: 4072 1279 488 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2566 2630 2823 3120 3126 928 Size: 971 667 64 193 297 6 3641 Fragments arranged by size: 3641 971 667 297 193 64 6 BbvCI CC'TCA_GC Cuts at: 2838 2838 Size: 5839 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4030 4064 1903 Size: 34 2093 34 3678 Fragments arranged by size: 3678 2093 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3731 4941 3731 Size: 1210 4629 BclI T'GATC_A Cuts at: 1548 2955 1548 Size: 1407 4432 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4450 136 Size: 107 122 71 631 605 2778 1525 Fragments arranged by size: 2778 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5834 5834 Size: 5839 BlpI GC'TnA_GC Cuts at: 3256 3256 Size: 5839 Bme1580I G_kGCm'C Cuts at: 1790 2183 2670 3900 5056 1790 Size: 393 487 1230 1156 2573 Fragments arranged by size: 2573 1230 1156 487 393 BmgBI CAC'GTC Cuts at: 1318 3097 1318 Size: 1779 4060 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2304 2697 3570 Size: 416 53 83 399 24 887 393 873 Cuts at: 3570 4528 442 Size: 958 1753 Fragments arranged by size: 1753 958 887 873 416 399 393 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5839 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3038 3038 Size: 5839 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2537 2734 2865 4499 1239 Size: 1298 197 131 1634 2579 Fragments arranged by size: 2579 1634 1298 197 131 Bpu10I CC'TnA_GC Cuts at: 2838 2868 2838 Size: 30 5809 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2221 3964 4829 5127 2221 Size: 1743 865 298 2933 Fragments arranged by size: 2933 1743 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2145 4502 882 Size: 617 646 2357 2219 Fragments arranged by size: 2357 2219 646 617 BsaAI yAC'GTr Cuts at: 493 1204 2592 2800 3630 493 Size: 711 1388 208 830 2702 Fragments arranged by size: 2702 1388 830 711 208 BsaBI GATnn'nnATC Cuts at: 2957 5833 2957 Size: 2876 2963 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3646 4028 4663 275 Size: 53 83 186 936 2113 382 635 1451 Fragments arranged by size: 2113 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2189 2540 2620 4272 4670 4944 5091 5502 2189 Size: 351 80 1652 398 274 147 411 2526 Fragments arranged by size: 2526 1652 411 398 351 274 147 80 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5614 Fragments arranged by size: 5614 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2826 1186 Size: 1640 4199 BseYI C'CCAG_C Cuts at: 2163 2494 5042 5538 2163 Size: 331 2548 496 2464 Fragments arranged by size: 2548 2464 496 331 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2484 3119 1163 Size: 573 748 635 3883 Fragments arranged by size: 3883 748 635 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3289 4050 5078 1540 Size: 275 186 1288 761 1028 2301 Fragments arranged by size: 2301 1288 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2985 3900 3985 5056 2985 Size: 915 85 1071 3768 Fragments arranged by size: 3768 1071 915 85 BsmI GAATG_Cn' Cuts at: 3350 3443 3350 Size: 93 5746 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2183 2670 2985 3158 3900 3985 5056 Size: 399 393 487 315 173 742 85 1071 Cuts at: 5056 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 487 399 393 315 173 85 BspEI T'CCGG_A Cuts at: 2620 5502 2620 Size: 2882 2957 BspHI T'CATG_A Cuts at: 3728 5458 3728 Size: 1730 4109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3194 844 Size: 2350 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3726 1394 Size: 429 1903 3507 Fragments arranged by size: 3507 1903 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2565 4334 4508 65 Size: 1183 1317 1769 174 1396 Fragments arranged by size: 1769 1396 1317 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4483 4670 1700 Size: 110 2673 187 2869 Fragments arranged by size: 2869 2673 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5839 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5839 BssSI C'ACGA_G Cuts at: 3899 4911 3899 Size: 1012 4827 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5839 BstBI TT'CG_AA Cuts at: 4643 4643 Size: 5839 BstEII G'GTnAC_C Cuts at: 1184 2102 2230 1184 Size: 918 128 4793 Fragments arranged by size: 4793 918 128 BstYI r'GATC_y Cuts at: 1972 2779 3169 3536 3921 3938 5390 5476 5488 Size: 807 390 367 385 17 1452 86 12 Cuts at: 5488 5834 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 807 390 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5287 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2944 487 Size: 683 56 1718 3382 Fragments arranged by size: 3382 1718 683 56 BtsI GCAGTG_nn' Cuts at: 2676 3355 4149 4169 2676 Size: 679 794 20 4346 Fragments arranged by size: 4346 794 679 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5839 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5804 DraI TTT'AAA Cuts at: 3143 3143 Size: 5839 DraIII CAC_nnn'GTG Cuts at: 2110 2110 Size: 5839 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4846 809 Size: 507 3530 1802 Fragments arranged by size: 3530 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3286 4175 5804 Size: 54 1425 50 275 18 1456 889 1629 Cuts at: 5804 8 Size: 43 Fragments arranged by size: 1629 1456 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3286 1537 Size: 275 1474 4090 Fragments arranged by size: 4090 1474 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2990 3769 1353 Size: 1637 779 3423 Fragments arranged by size: 3423 1637 779 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4426 4800 4946 387 Size: 1064 138 2837 374 146 1280 Fragments arranged by size: 2837 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2058 2246 2537 2734 2865 3902 4499 Size: 393 426 188 291 197 131 1037 597 Cuts at: 4499 5286 5554 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1037 787 597 426 393 291 268 197 188 131 EcoNI CCTnn'n_nnAGG Cuts at: 2621 2621 Size: 5839 EcoO109I rG'GnC_Cy Cuts at: 2666 2666 Size: 5839 EcoRI G'AATT_C Cuts at: 3148 3526 3148 Size: 378 5461 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3143 3143 Size: 5839 FspI TGC'GCA Cuts at: 4345 4345 Size: 5839 HaeII r_GCGC'y Cuts at: 1678 2207 4986 1678 Size: 529 2779 2531 Fragments arranged by size: 2779 2531 529 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2271 2303 2771 2803 2813 Size: 32 135 32 699 32 468 32 10 Cuts at: 2813 2845 2967 2999 4480 4512 4554 4586 1373 Size: 32 122 32 1481 32 42 32 2626 Fragments arranged by size: 2626 1481 699 468 135 122 42 32 32 32 32 32 32 32 32 10 HincII GTy'rAC Cuts at: 669 1657 1938 3183 3429 669 Size: 988 281 1245 246 3079 Fragments arranged by size: 3079 1245 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5839 HpaI GTT'AAC Cuts at: 3429 3429 Size: 5839 KpnI G_GTAC'C Cuts at: 3164 3164 Size: 5839 MfeI C'AATT_G Cuts at: 3438 3438 Size: 5839 MluI A'CGCG_T Cuts at: 5715 5715 Size: 5839 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2531 4953 5137 1305 Size: 1226 2422 184 2007 Fragments arranged by size: 2422 2007 1226 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4017 Fragments arranged by size: 4017 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2348 2918 3797 4156 4315 518 Size: 648 1182 570 879 359 159 2042 Fragments arranged by size: 2042 1182 879 648 570 359 159 MspA1I CmG'CkG Cuts at: 1606 2325 2498 3255 3932 5080 5325 1606 Size: 719 173 757 677 1148 245 2120 Fragments arranged by size: 2120 1148 757 719 677 245 173 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5287 NdeI CA'TA_TG Cuts at: 387 387 Size: 5839 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5839 NotI GC'GGCC_GC Cuts at: 3286 3286 Size: 5839 NspI r_CATG'y Cuts at: 1132 1229 2161 3197 4742 1132 Size: 97 932 1036 1545 2229 Fragments arranged by size: 2229 1545 1036 932 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5839 PciI A'CATG_T Cuts at: 4738 4738 Size: 5839 PflMI CCAn_nnn'nTGG Cuts at: 1108 2775 1108 Size: 1667 4172 PfoI T'CCnGG_A Cuts at: 2711 2711 Size: 5839 PmeI GTTT'AAAC Cuts at: 3143 3143 Size: 5839 PmlI CAC'GTG Cuts at: 2592 2800 2592 Size: 208 5631 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3908 3940 5421 5453 3908 Size: 32 1481 32 4294 Fragments arranged by size: 4294 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2066 1613 Size: 453 5386 PsiI TTA'TAA Cuts at: 3409 3409 Size: 5839 PspOMI G'GGCC_C Cuts at: 2666 2666 Size: 5839 PstI C_TGCA'G Cuts at: 830 2683 3191 830 Size: 1853 508 3478 Fragments arranged by size: 3478 1853 508 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5839 PvuII CAG'CTG Cuts at: 2498 2498 Size: 5839 SalI G'TCGA_C Cuts at: 1936 3181 1936 Size: 1245 4594 SapI GCTCTTCn'nnn_ Cuts at: 2990 2990 Size: 5839 SbfI CC_TGCA'GG Cuts at: 3191 3191 Size: 5839 ScaI AGT'ACT Cuts at: 4087 5799 4087 Size: 1712 4127 SfcI C'TryA_G Cuts at: 826 1045 2396 2679 3187 4322 5003 5194 826 Size: 219 1351 283 508 1135 681 191 1471 Fragments arranged by size: 1471 1351 1135 681 508 283 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5839 SmaI CCC'GGG Cuts at: 1872 3166 1872 Size: 1294 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2236 3943 4844 5106 820 Size: 197 926 293 1707 901 262 1553 Fragments arranged by size: 1707 1553 926 901 293 262 197 SnaBI TAC'GTA Cuts at: 493 3630 493 Size: 3137 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5839 SphI G_CATG'C Cuts at: 1132 3197 1132 Size: 2065 3774 SspI AAT'ATT Cuts at: 5 52 3763 5 Size: 47 3711 2081 Fragments arranged by size: 3711 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5839 StyI C'CwwG_G Cuts at: 513 1065 2970 3278 513 Size: 552 1905 308 3074 Fragments arranged by size: 3074 1905 552 308 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3866 3883 4036 4221 4666 325 Size: 1255 2286 17 153 185 445 1498 Fragments arranged by size: 2286 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2127 2331 4085 Size: 274 80 33 51 1443 150 204 1754 Cuts at: 4085 5797 96 Size: 1712 138 Fragments arranged by size: 1754 1712 1443 274 204 150 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2214 2246 2803 2835 5799 5831 2214 Size: 32 557 32 2964 32 2222 Fragments arranged by size: 2964 2222 557 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2744 1316 Size: 1428 4411 XbaI T'CTAG_A Cuts at: 3175 3175 Size: 5839 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5839 XmaI C'CCGG_G Cuts at: 1870 3164 1870 Size: 1294 4545 XmnI GAAnn'nnTTC Cuts at: 3968 3968 Size: 5839 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3647 276 Size: 53 83 186 936 2113 2468 Fragments arranged by size: 2468 2113 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI