(Linear) (Six-base) MAPSORT of: KIEE2451.seq Check: 4656 from: 1 to: 1134 KIEE2451.orf With 182 enzymes: SgfI * February 5, 2013 10:21 .. AccI GT'mk_AC Cuts at: 0 724 1134 Size: 724 410 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 53 241 1134 Size: 53 188 893 Fragments arranged by size: 893 188 53 AflIII A'CryG_T Cuts at: 0 584 794 1134 Size: 584 210 340 Fragments arranged by size: 584 340 210 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 361 603 1134 Size: 361 242 531 Fragments arranged by size: 531 361 242 AlwNI CAG_nnn'CTG Cuts at: 0 76 688 862 1134 Size: 76 612 174 272 Fragments arranged by size: 612 272 174 76 ApaI G_GGCC'C Cuts at: 0 665 1134 Size: 665 469 AvaI C'yCGr_G Cuts at: 0 270 643 1134 Size: 270 373 491 Fragments arranged by size: 491 373 270 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 842 875 1134 Size: 842 33 259 Fragments arranged by size: 842 259 33 BanII G_rGCy'C Cuts at: 0 665 1134 Size: 665 469 BbsI GAAGACnn'nnnn_ Cuts at: 0 561 625 818 1115 1121 1134 Size: 561 64 193 297 6 13 Fragments arranged by size: 561 297 193 64 13 6 BbvCI CC'TCA_GC Cuts at: 0 833 1134 Size: 833 301 BclI T'GATC_A Cuts at: 0 950 1134 Size: 950 184 Bme1580I G_kGCm'C Cuts at: 0 178 665 1134 Size: 178 487 469 Fragments arranged by size: 487 469 178 BmgBI CAC'GTC Cuts at: 0 1092 1134 Size: 1092 42 BmrI ACTGGGnnnn_n' Cuts at: 0 299 692 1134 Size: 299 393 442 Fragments arranged by size: 442 393 299 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1033 1134 Size: 1033 101 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 532 729 860 1134 Size: 532 197 131 274 Fragments arranged by size: 532 274 197 131 Bpu10I CC'TnA_GC Cuts at: 0 833 863 1134 Size: 833 30 271 Fragments arranged by size: 833 271 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 216 1134 Size: 216 918 BsaI GGTCTCn'nnnn_ Cuts at: 0 140 1134 Size: 140 994 BsaAI yAC'GTr Cuts at: 0 587 795 1134 Size: 587 208 339 Fragments arranged by size: 587 339 208 BsaBI GATnn'nnATC Cuts at: 0 952 1134 Size: 952 182 BsaWI w'CCGG_w Cuts at: 0 184 535 615 1134 Size: 184 351 80 519 Fragments arranged by size: 519 351 184 80 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 821 1134 Size: 821 313 BseYI C'CCAG_C Cuts at: 0 158 489 1134 Size: 158 331 645 Fragments arranged by size: 645 331 158 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 479 1114 1134 Size: 479 635 20 Fragments arranged by size: 635 479 20 BsiHKAI G_wGCw'C Cuts at: 0 980 1134 Size: 980 154 Bsp1286I G_dGCh'C Cuts at: 0 178 665 980 1134 Size: 178 487 315 154 Fragments arranged by size: 487 315 178 154 BspEI T'CCGG_A Cuts at: 0 615 1134 Size: 615 519 BsrDI GCAATG_nn' Cuts at: 0 560 1134 Size: 560 574 BstEII G'GTnAC_C Cuts at: 0 97 225 1134 Size: 97 128 909 Fragments arranged by size: 909 128 97 BstYI r'GATC_y Cuts at: 0 774 1134 Size: 774 360 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 939 1134 Size: 939 195 BtsI GCAGTG_nn' Cuts at: 0 671 1134 Size: 671 463 DraIII CAC_nnn'GTG Cuts at: 0 105 1134 Size: 105 1029 EarI CTCTTCn'nnn_ Cuts at: 0 985 1134 Size: 985 149 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 53 241 532 729 860 1134 Size: 53 188 291 197 131 274 Fragments arranged by size: 291 274 197 188 131 53 EcoNI CCTnn'n_nnAGG Cuts at: 0 616 1134 Size: 616 518 EcoO109I rG'GnC_Cy Cuts at: 0 661 1134 Size: 661 473 HaeII r_GCGC'y Cuts at: 0 202 1134 Size: 202 932 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 266 298 766 798 808 840 962 994 Size: 266 32 468 32 10 32 122 32 Cuts at: 994 1134 Size: 140 Fragments arranged by size: 468 266 140 122 32 32 32 32 10 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 526 1134 Size: 526 608 MslI CAynn'nnrTG Cuts at: 0 343 913 1134 Size: 343 570 221 Fragments arranged by size: 570 343 221 MspA1I CmG'CkG Cuts at: 0 320 493 1134 Size: 320 173 641 Fragments arranged by size: 641 320 173 NspI r_CATG'y Cuts at: 0 156 1134 Size: 156 978 PflMI CCAn_nnn'nTGG Cuts at: 0 770 1134 Size: 770 364 PfoI T'CCnGG_A Cuts at: 0 706 1134 Size: 706 428 PmlI CAC'GTG Cuts at: 0 587 795 1134 Size: 587 208 339 Fragments arranged by size: 587 339 208 PshAI GACnn'nnGTC Cuts at: 0 61 1134 Size: 61 1073 PspOMI G'GGCC_C Cuts at: 0 661 1134 Size: 661 473 PstI C_TGCA'G Cuts at: 0 678 1134 Size: 678 456 PvuII CAG'CTG Cuts at: 0 493 1134 Size: 493 641 SapI GCTCTTCn'nnn_ Cuts at: 0 985 1134 Size: 985 149 SfcI C'TryA_G Cuts at: 0 391 674 1134 Size: 391 283 460 Fragments arranged by size: 460 391 283 SmlI C'TyrA_G Cuts at: 0 231 1134 Size: 231 903 StyI C'CwwG_G Cuts at: 0 965 1134 Size: 965 169 TatI w'GTAC_w Cuts at: 0 122 326 1134 Size: 122 204 808 Fragments arranged by size: 808 204 122 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 209 241 798 830 1134 Size: 209 32 557 32 304 Fragments arranged by size: 557 304 209 32 32 Tth111I GACn'n_nGTC Cuts at: 0 739 1134 Size: 739 395 Enzymes that do cut: AccI AcuI AflIII AlfI AlwNI ApaI AvaI BaeI BanII BbsI BbvCI BclI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BsrDI BstEII BstYI BtgZI BtsI DraIII EarI Eco57MI EcoNI EcoO109I HaeII Hin4I MmeI MslI MspA1I NspI PflMI PfoI PmlI PshAI PspOMI PstI PvuII SapI SfcI SmlI StyI TatI TstI Tth111I Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BamHI BanI BbeI BcgI BciVI BfrBI BglI BglII BlpI BmtI BsaHI BsaXI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PpiI PpuMI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII XbaI XcmI XhoI XmaI XmnI ZraI