Restriction map for
pFN21AE6478
(Circular) (Six-base) MAPSORT of: pFN21AE6478.seq Check: 6149 from: 1 to: 6154
pFN21AE6478 6154 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3964 278
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3497 1937
Size: 1560 4594
Acc65I G'GTAC_C
Cuts at: 3475 3475
Size: 6154
AclI AA'CG_TT
Cuts at: 1563 4281 4654 1563
Size: 2718 373 3063
Fragments arranged by size:
3063 2718 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2115 4217 5601 5869 1632
Size: 483 2102 1384 268 1917
Fragments arranged by size:
2102 1917 1384 483 268
AfeI AGC'GCT
Cuts at: 2336 2336
Size: 6154
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5957
AflIII A'CryG_T
Cuts at: 1203 5053 6030 1203
Size: 3850 977 1327
Fragments arranged by size:
3850 1327 977
AgeI A'CCGG_T
Cuts at: 4985 4985
Size: 6154
AhdI GACnn_n'nnGTC
Cuts at: 2065 4883 2065
Size: 2818 3336
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6154
AlwNI CAG_nnn'CTG
Cuts at: 3391 3897 5469 3391
Size: 506 1572 4076
Fragments arranged by size:
4076 1572 506
ApaI G_GGCC'C
Cuts at: 2267 2267
Size: 6154
ApaLI G'TGCA_C
Cuts at: 3058 4211 5367 3058
Size: 1153 1156 3845
Fragments arranged by size:
3845 1156 1153
ApoI r'AATT_y
Cuts at: 1493 3463 3689 3841 5950 1493
Size: 1970 226 152 2109 1697
Fragments arranged by size:
2109 1970 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4708 160
Size: 872 3676 1606
Fragments arranged by size:
3676 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
AvaI C'yCGr_G
Cuts at: 1870 1943 3117 3479 1870
Size: 73 1174 362 4545
Fragments arranged by size:
4545 1174 362 73
BamHI G'GATC_C
Cuts at: 2217 3484 3851 2217
Size: 1267 367 4520
Fragments arranged by size:
4520 1267 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3248 3475 4930 618
Size: 325 217 627 1461 227 1455 1842
Fragments arranged by size:
1842 1461 1455 627 325 227 217
BanII G_rGCy'C
Cuts at: 1391 2267 3239 3317 3473 1391
Size: 876 972 78 156 4072
Fragments arranged by size:
4072 972 876 156 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2159 2798 3420 928
Size: 971 260 639 622 3662
Fragments arranged by size:
3662 971 639 622 260
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4345 4379 1903
Size: 34 2408 34 3678
Fragments arranged by size:
3678 2408 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4046 5256 4046
Size: 1210 4944
BclI T'GATC_A
Cuts at: 1548 3342 1548
Size: 1794 4360
BfrBI ATG'CAT
Cuts at: 2389 2389
Size: 6154
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4765 136
Size: 107 122 71 631 605 3093 1525
Fragments arranged by size:
3093 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6149 6149
Size: 6154
BlpI GC'TnA_GC
Cuts at: 3571 3571
Size: 6154
Bme1580I G_kGCm'C
Cuts at: 1790 2213 2267 2332 3062 3139 4215 5371 1790
Size: 423 54 65 730 77 1076 1156 2573
Fragments arranged by size:
2573 1156 1076 730 423 77 65 54
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6154
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2446 3885 4843
Size: 416 53 83 399 24 1029 1439 958
Cuts at: 4843 442
Size: 1753
Fragments arranged by size:
1753 1439 1029 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2405 3302 4814 1239
Size: 1166 897 1512 2579
Fragments arranged by size:
2579 1512 1166 897
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3389 4279 5144 5442 3389
Size: 890 865 298 4101
Fragments arranged by size:
4101 890 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4817 882
Size: 617 3318 2219
Fragments arranged by size:
3318 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3945 493
Size: 711 2741 2702
Fragments arranged by size:
2741 2702 711
BsaBI GATnn'nnATC
Cuts at: 3347 6148 3347
Size: 2801 3353
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3336 3961 4343 4978
Size: 53 83 186 936 1803 625 382 635
Cuts at: 4978 275
Size: 1451
Fragments arranged by size:
1803 1451 936 635 625 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2658 4587 4985 5259 5406 5817 2658
Size: 1929 398 274 147 411 2995
Fragments arranged by size:
2995 1929 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2606 2636 1178
Size: 30 165 30 1203 30 4696
Fragments arranged by size:
4696 1203 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6154
BseYI C'CCAG_C
Cuts at: 2331 2762 2890 3132 5357 5853 2331
Size: 431 128 242 2225 496 2632
Fragments arranged by size:
2632 2225 496 431 242 128
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5581
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3604 4365 5393 1540
Size: 275 186 1603 761 1028 2301
Fragments arranged by size:
2301 1603 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3062 4215 4300 5371 3062
Size: 1153 85 1071 3845
Fragments arranged by size:
3845 1153 1071 85
BsmI GAATG_Cn'
Cuts at: 2881 3665 3758 2881
Size: 784 93 5277
Fragments arranged by size:
5277 784 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2570 2570
Size: 6154
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2213 2267 2332 3062 3139 3239 3317
Size: 399 423 54 65 730 77 100 78
Cuts at: 3317 3473 4215 4300 5371 1391
Size: 156 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 730 423 399 156 100
85 78 77 65 54
BspEI T'CCGG_A
Cuts at: 2658 5817 2658
Size: 3159 2995
BspHI T'CATG_A
Cuts at: 4043 5773 4043
Size: 1730 4424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2967 3009 3509 844
Size: 2123 42 500 3489
Fragments arranged by size:
3489 2123 500 42
BsrBI CCG'CTC
Cuts at: 1394 1823 3277 4041 1394
Size: 429 1454 764 3507
Fragments arranged by size:
3507 1454 764 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2292 3042 4649 4823 65
Size: 1183 1044 750 1607 174 1396
Fragments arranged by size:
1607 1396 1183 1044 750 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2766 3047 4798 4985 1700
Size: 110 956 281 1751 187 2869
Fragments arranged by size:
2869 1751 956 281 187 110
BsrGI T'GTAC_A
Cuts at: 96 2496 96
Size: 2400 3754
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6154
BssSI C'ACGA_G
Cuts at: 4214 5226 4214
Size: 1012 5142
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3057 794
Size: 2263 3891
BstBI TT'CG_AA
Cuts at: 4958 4958
Size: 6154
BstEII G'GTnAC_C
Cuts at: 1184 3286 1184
Size: 2102 4052
BstXI CCAn_nnnn'nTGG
Cuts at: 2056 2092 2056
Size: 36 6118
BstYI r'GATC_y
Cuts at: 1972 2217 2670 3148 3484 3851 4236 4253 5705
Size: 245 453 478 336 367 385 17 1452
Cuts at: 5705 5791 5803 6149 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 478 453 385 367 346 336
245 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2666 2666
Size: 6154
BtgI C'CryG_G
Cuts at: 513 1065 2598 513
Size: 552 1533 4069
Fragments arranged by size:
4069 1533 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5415
Fragments arranged by size:
5415 683 56
BtsI GCAGTG_nn'
Cuts at: 3137 3670 4464 4484 3137
Size: 533 794 20 4807
Fragments arranged by size:
4807 794 533 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6154
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3427 3462 529
Size: 35 2863 35 3221
Fragments arranged by size:
3221 2863 35 35
DraI TTT'AAA
Cuts at: 2523 3458 2523
Size: 935 5219
DraIII CAC_nnn'GTG
Cuts at: 2194 2194
Size: 6154
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5161 809
Size: 507 3845 1802
Fragments arranged by size:
3845 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2694 3192 3601
Size: 54 1425 50 275 18 864 498 409
Cuts at: 3601 4490 6119 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 864 498 409 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3601 1537
Size: 275 1789 4090
Fragments arranged by size:
4090 1789 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2810 4084 1353
Size: 1457 1274 3423
Fragments arranged by size:
3423 1457 1274
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4741 5115 5261 387
Size: 1064 138 3152 374 146 1280
Fragments arranged by size:
3152 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2405 3302 4217 4814 5601 5869
Size: 393 483 290 897 915 597 787 268
Cuts at: 5869 1239
Size: 1524
Fragments arranged by size:
1524 915 897 787 597 483 393 290
268
EcoO109I rG'GnC_Cy
Cuts at: 2030 3378 3388 2030
Size: 1348 10 4796
Fragments arranged by size:
4796 1348 10
EcoRI G'AATT_C
Cuts at: 3463 3841 3463
Size: 378 5776
FspI TGC'GCA
Cuts at: 4660 4660
Size: 6154
HaeII r_GCGC'y
Cuts at: 1678 2338 5301 1678
Size: 660 2963 2531
Fragments arranged by size:
2963 2531 660
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2051 2083 2217 2249 2785
Size: 32 135 32 479 32 134 32 536
Cuts at: 2785 2817 2961 2993 4795 4827 4869 4901 1373
Size: 32 144 32 1802 32 42 32 2626
Fragments arranged by size:
2626 1802 536 479 144 135 134 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3126 3498 3744 669
Size: 988 281 1188 372 246 3079
Fragments arranged by size:
3079 1188 988 372 281 246
HindIII A'AGCT_T
Cuts at: 748 3032 748
Size: 2284 3870
HpaI GTT'AAC
Cuts at: 3744 3744
Size: 6154
KpnI G_GTAC'C
Cuts at: 3479 3479
Size: 6154
MfeI C'AATT_G
Cuts at: 3753 3753
Size: 6154
MluI A'CGCG_T
Cuts at: 6030 6030
Size: 6154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2073 3456 5268 5452 1305
Size: 768 1383 1812 184 2007
Fragments arranged by size:
2007 1812 1383 768 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2696 3194 10
Size: 54 1425 343 864 498 2970
Fragments arranged by size:
2970 1425 864 498 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2168 2258 2384 2705 2843 2939
Size: 648 843 159 90 126 321 138 96
Cuts at: 2939 4112 4471 4630 518
Size: 1173 359 159 2042
Fragments arranged by size:
2042 1173 843 648 359 321 159 159
138 126 96 90
MspA1I CmG'CkG
Cuts at: 1606 3570 4247 5395 5640 1606
Size: 1964 677 1148 245 2120
Fragments arranged by size:
2120 1964 1148 677 245
NaeI GCC'GGC
Cuts at: 3049 3049
Size: 6154
NcoI C'CATG_G
Cuts at: 513 1065 2598 513
Size: 552 1533 4069
Fragments arranged by size:
4069 1533 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6154
NgoMIV G'CCGG_C
Cuts at: 3047 3047
Size: 6154
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6154
NotI GC'GGCC_GC
Cuts at: 3601 3601
Size: 6154
NruI TCG'CGA
Cuts at: 2608 2608
Size: 6154
NsiI A_TGCA'T
Cuts at: 2391 2391
Size: 6154
NspI r_CATG'y
Cuts at: 1132 1229 2620 2710 3186 3512 5057 1132
Size: 97 1391 90 476 326 1545 2229
Fragments arranged by size:
2229 1545 1391 476 326 97 90
PasI CC'CwG_GG
Cuts at: 1348 2035 1348
Size: 687 5467
PciI A'CATG_T
Cuts at: 5053 5053
Size: 6154
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2415 3391 1108
Size: 1307 976 3871
Fragments arranged by size:
3871 1307 976
PfoI T'CCnGG_A
Cuts at: 3279 3279
Size: 6154
PmeI GTTT'AAAC
Cuts at: 3458 3458
Size: 6154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2090 2122 4223 4255 5736 5768 2090
Size: 32 2101 32 1481 32 2476
Fragments arranged by size:
2476 2101 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2030 3378 2030
Size: 1348 4806
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6154
PsiI TTA'TAA
Cuts at: 3724 3724
Size: 6154
PspOMI G'GGCC_C
Cuts at: 2263 2263
Size: 6154
PstI C_TGCA'G
Cuts at: 830 3075 3091 3506 830
Size: 2245 16 415 3478
Fragments arranged by size:
3478 2245 415 16
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6154
SalI G'TCGA_C
Cuts at: 1936 3496 1936
Size: 1560 4594
SanDI GG'GwC_CC
Cuts at: 2030 2030
Size: 6154
SbfI CC_TGCA'GG
Cuts at: 3506 3506
Size: 6154
ScaI AGT'ACT
Cuts at: 2023 4402 6114 2023
Size: 2379 1712 2063
Fragments arranged by size:
2379 2063 1712
SexAI A'CCwGG_T
Cuts at: 2190 3216 2190
Size: 1026 5128
SfcI C'TryA_G
Cuts at: 826 1045 3071 3087 3502 4637 5318 5509 826
Size: 219 2026 16 415 1135 681 191 1471
Fragments arranged by size:
2026 1471 1135 681 415 219 191 16
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6154
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6154
SmaI CCC'GGG
Cuts at: 1872 3119 3481 1872
Size: 1247 362 4545
Fragments arranged by size:
4545 1247 362
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3404 4258 5159 5421 820
Size: 197 926 1461 854 901 262 1553
Fragments arranged by size:
1553 1461 926 901 854 262 197
SnaBI TAC'GTA
Cuts at: 493 3945 493
Size: 3452 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6154
SphI G_CATG'C
Cuts at: 1132 2710 3512 1132
Size: 1578 802 3774
Fragments arranged by size:
3774 1578 802
SspI AAT'ATT
Cuts at: 5 52 4078 5
Size: 47 4026 2081
Fragments arranged by size:
4026 2081 47
StuI AGG'CCT
Cuts at: 1517 2180 1517
Size: 663 5491
StyI C'CwwG_G
Cuts at: 513 1065 2157 2598 3593 513
Size: 552 1092 441 995 3074
Fragments arranged by size:
3074 1092 995 552 441
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2489 4181 4198 4351 4536 4981 325
Size: 1255 909 1692 17 153 185 445 1498
Fragments arranged by size:
1692 1498 1255 909 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2021 2496 2736
Size: 274 80 33 51 1443 44 475 240
Cuts at: 2736 4400 6112 96
Size: 1664 1712 138
Fragments arranged by size:
1712 1664 1443 475 274 240 138 80
51 44 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3413 3445 6114 6146 3413
Size: 32 2669 32 3421
Fragments arranged by size:
3421 2669 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3218 1316
Size: 1902 4252
XbaI T'CTAG_A
Cuts at: 3490 3490
Size: 6154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2902 2902
Size: 6154
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6154
XmaI C'CCGG_G
Cuts at: 1870 3117 3479 1870
Size: 1247 362 4545
Fragments arranged by size:
4545 1247 362
XmnI GAAnn'nnTTC
Cuts at: 4283 4283
Size: 6154
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3962 276
Size: 53 83 186 936 2428 2468
Fragments arranged by size:
2468 2428 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BstZ17I EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NarI PacI PmlI PspXI PsrI PvuII
RsrII SacI SacII SapI SfiI SfoI SrfI SwaI