(Linear) (Six-base) MAPSORT of: KIEE6478.seq Check: 7865 from: 1 to: 1449 KIEE6478.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 110 1449 Size: 110 1339 AfeI AGC'GCT Cuts at: 0 331 1449 Size: 331 1118 AhdI GACnn_n'nnGTC Cuts at: 0 60 1449 Size: 60 1389 AlwNI CAG_nnn'CTG Cuts at: 0 1386 1449 Size: 1386 63 ApaI G_GGCC'C Cuts at: 0 262 1449 Size: 262 1187 ApaLI G'TGCA_C Cuts at: 0 1053 1449 Size: 1053 396 AvaI C'yCGr_G Cuts at: 0 1112 1449 Size: 1112 337 BamHI G'GATC_C Cuts at: 0 212 1449 Size: 212 1237 BanI G'GyrC_C Cuts at: 0 1243 1449 Size: 1243 206 BanII G_rGCy'C Cuts at: 0 262 1234 1312 1449 Size: 262 972 78 137 Fragments arranged by size: 972 262 137 78 BbsI GAAGACnn'nnnn_ Cuts at: 0 154 793 1415 1449 Size: 154 639 622 34 Fragments arranged by size: 639 622 154 34 BclI T'GATC_A Cuts at: 0 1337 1449 Size: 1337 112 BfrBI ATG'CAT Cuts at: 0 384 1449 Size: 384 1065 Bme1580I G_kGCm'C Cuts at: 0 208 262 327 1057 1134 1449 Size: 208 54 65 730 77 315 Fragments arranged by size: 730 315 208 77 65 54 BmrI ACTGGGnnnn_n' Cuts at: 0 441 1449 Size: 441 1008 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 400 1297 1449 Size: 400 897 152 Fragments arranged by size: 897 400 152 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1384 1449 Size: 1384 65 BsaBI GATnn'nnATC Cuts at: 0 1342 1449 Size: 1342 107 BsaHI Gr'CG_yC Cuts at: 0 1331 1449 Size: 1331 118 BsaWI w'CCGG_w Cuts at: 0 653 1449 Size: 653 796 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 601 631 1449 Size: 601 30 818 Fragments arranged by size: 818 601 30 BseYI C'CCAG_C Cuts at: 0 326 757 885 1127 1449 Size: 326 431 128 242 322 Fragments arranged by size: 431 326 322 242 128 BsiHKAI G_wGCw'C Cuts at: 0 1057 1449 Size: 1057 392 BsmI GAATG_Cn' Cuts at: 0 876 1449 Size: 876 573 BsmBI CGTCTCn'nnnn_ Cuts at: 0 565 1449 Size: 565 884 Bsp1286I G_dGCh'C Cuts at: 0 208 262 327 1057 1134 1234 1312 1449 Size: 208 54 65 730 77 100 78 137 Fragments arranged by size: 730 208 137 100 78 77 65 54 BspEI T'CCGG_A Cuts at: 0 653 1449 Size: 653 796 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 962 1004 1449 Size: 962 42 445 Fragments arranged by size: 962 445 42 BsrBI CCG'CTC Cuts at: 0 1272 1449 Size: 1272 177 BsrDI GCAATG_nn' Cuts at: 0 287 1037 1449 Size: 287 750 412 Fragments arranged by size: 750 412 287 BsrFI r'CCGG_y Cuts at: 0 761 1042 1449 Size: 761 281 407 Fragments arranged by size: 761 407 281 BsrGI T'GTAC_A Cuts at: 0 491 1449 Size: 491 958 BstAPI GCAn_nnn'nTGC Cuts at: 0 1052 1449 Size: 1052 397 BstEII G'GTnAC_C Cuts at: 0 1281 1449 Size: 1281 168 BstXI CCAn_nnnn'nTGG Cuts at: 0 51 87 1449 Size: 51 36 1362 Fragments arranged by size: 1362 51 36 BstYI r'GATC_y Cuts at: 0 212 665 1143 1449 Size: 212 453 478 306 Fragments arranged by size: 478 453 306 212 Bsu36I CC'TnA_GG Cuts at: 0 661 1449 Size: 661 788 BtgI C'CryG_G Cuts at: 0 593 1449 Size: 593 856 BtsI GCAGTG_nn' Cuts at: 0 1132 1449 Size: 1132 317 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1422 1449 Size: 1422 27 DraI TTT'AAA Cuts at: 0 518 1449 Size: 518 931 DraIII CAC_nnn'GTG Cuts at: 0 189 1449 Size: 189 1260 EaeI y'GGCC_r Cuts at: 0 689 1187 1449 Size: 689 498 262 Fragments arranged by size: 689 498 262 EarI CTCTTCn'nnn_ Cuts at: 0 805 1449 Size: 805 644 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 110 400 1297 1449 Size: 110 290 897 152 Fragments arranged by size: 897 290 152 110 EcoO109I rG'GnC_Cy Cuts at: 0 25 1373 1383 1449 Size: 25 1348 10 66 Fragments arranged by size: 1348 66 25 10 HaeII r_GCGC'y Cuts at: 0 333 1449 Size: 333 1116 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 46 78 212 244 780 812 956 988 Size: 46 32 134 32 536 32 144 32 Cuts at: 988 1449 Size: 461 Fragments arranged by size: 536 461 144 134 46 32 32 32 32 HincII GTy'rAC Cuts at: 0 1121 1449 Size: 1121 328 HindIII A'AGCT_T Cuts at: 0 1027 1449 Size: 1027 422 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 68 1449 Size: 68 1381 MscI TGG'CCA Cuts at: 0 691 1189 1449 Size: 691 498 260 Fragments arranged by size: 691 498 260 MslI CAynn'nnrTG Cuts at: 0 163 253 379 700 838 934 1449 Size: 163 90 126 321 138 96 515 Fragments arranged by size: 515 321 163 138 126 96 90 NaeI GCC'GGC Cuts at: 0 1044 1449 Size: 1044 405 NcoI C'CATG_G Cuts at: 0 593 1449 Size: 593 856 NgoMIV G'CCGG_C Cuts at: 0 1042 1449 Size: 1042 407 NruI TCG'CGA Cuts at: 0 603 1449 Size: 603 846 NsiI A_TGCA'T Cuts at: 0 386 1449 Size: 386 1063 NspI r_CATG'y Cuts at: 0 615 705 1181 1449 Size: 615 90 476 268 Fragments arranged by size: 615 476 268 90 PasI CC'CwG_GG Cuts at: 0 30 1449 Size: 30 1419 PflMI CCAn_nnn'nTGG Cuts at: 0 410 1386 1449 Size: 410 976 63 Fragments arranged by size: 976 410 63 PfoI T'CCnGG_A Cuts at: 0 1274 1449 Size: 1274 175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 85 117 1449 Size: 85 32 1332 Fragments arranged by size: 1332 85 32 PpuMI rG'GwC_Cy Cuts at: 0 25 1373 1449 Size: 25 1348 76 Fragments arranged by size: 1348 76 25 PspOMI G'GGCC_C Cuts at: 0 258 1449 Size: 258 1191 PstI C_TGCA'G Cuts at: 0 1070 1086 1449 Size: 1070 16 363 Fragments arranged by size: 1070 363 16 SanDI GG'GwC_CC Cuts at: 0 25 1449 Size: 25 1424 ScaI AGT'ACT Cuts at: 0 18 1449 Size: 18 1431 SexAI A'CCwGG_T Cuts at: 0 185 1211 1449 Size: 185 1026 238 Fragments arranged by size: 1026 238 185 SfcI C'TryA_G Cuts at: 0 1066 1082 1449 Size: 1066 16 367 Fragments arranged by size: 1066 367 16 SmaI CCC'GGG Cuts at: 0 1114 1449 Size: 1114 335 SmlI C'TyrA_G Cuts at: 0 1399 1449 Size: 1399 50 SphI G_CATG'C Cuts at: 0 705 1449 Size: 705 744 StuI AGG'CCT Cuts at: 0 175 1449 Size: 175 1274 StyI C'CwwG_G Cuts at: 0 152 593 1449 Size: 152 441 856 Fragments arranged by size: 856 441 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 484 1449 Size: 484 965 TatI w'GTAC_w Cuts at: 0 16 491 731 1449 Size: 16 475 240 718 Fragments arranged by size: 718 475 240 16 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1408 1440 1449 Size: 1408 32 9 Fragments arranged by size: 1408 32 9 Tth111I GACn'n_nGTC Cuts at: 0 1213 1449 Size: 1213 236 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 897 1449 Size: 897 552 XmaI C'CCGG_G Cuts at: 0 1112 1449 Size: 1112 337 Enzymes that do cut: AcuI AfeI AhdI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbsI BclI BfrBI Bme1580I BmrI BpmI BpuEI BsaBI BsaHI BsaWI BsaXI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DraI DraIII EaeI EarI Eco57MI EcoO109I HaeII Hin4I HincII HindIII MmeI MscI MslI NaeI NcoI NgoMIV NruI NsiI NspI PasI PflMI PfoI PpiI PpuMI PspOMI PstI SanDI ScaI SexAI SfcI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI BseRI BsgI BsiEI BsiWI BspHI BssHII BssSI BstBI BstZ17I BtgZI ClaI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HpaI KasI KpnI MfeI MluI MspA1I NarI NdeI NheI NotI PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SapI SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmnI ZraI