################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI BamHI BbvCI BcgI BfrI BsaBI BseAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I HgaI HindIII HpaI KpnI MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SbfI SexAI SfiI SfuI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 778 CGCG CG^CG Alw44I 1816 GTGCAC G^TGCAC ApaLI 1816 GTGCAC G^TGCAC AviII 1823 TGCGCA TGC^GCA AvrII 306 CCTAGG C^CTAGG BbrPI 1404 CACGTG CAC^GTG BciVI 692 GTATCC GTATCC(6/5) BclI 404 TGATCA T^GATCA BglI 1777 GCCACGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 881 AGATCT A^GATCT BlnI 306 CCTAGG C^CTAGG BlpI 1603 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1603 GCTGAGC GC^T[ACGT]AGC BsaI 1213 GAGACC GGTCTC(1/5) BsaWI 1757 ACCGGA [AT]^CCGG[AT] BsiEI 1720 CGGCCG CG[GA][CT]^CG BsmI 1433 GCATTC GAATGC(1/-1) BspHI 1643 TCATGA T^CATGA BspLU11I 1040 ACATGT A^CATGT BsrBI 1699 GAGCGG CCGCTC(-3/-3) BssSI 226 CTCGTG CACGAG(-5/-1) BstUI 778 CGCG CG^CG CelII 1603 GCTGAGC GC^T[ACGT]AGC DraIII 1401 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1720 CGGCCG C^GGCCG EclXI 1720 CGGCCG C^GGCCG Eco52I 1720 CGGCCG C^GGCCG FauI 1593 CCCGC CCCGC(4/6) FbaI 404 TGATCA T^GATCA FseI 1721 GGCCGGCC GGCCGG^CC FspI 1823 TGCGCA TGC^GCA HincII 79 GTCGAC GT[CT]^[GA]AC HindII 79 GTCGAC GT[CT]^[GA]AC KspI 777 CCGCGG CCGC^GG MvnI 778 CGCG CG^CG NcoI 1727 CCATGG C^CATGG PmaCI 1404 CACGTG CAC^GTG PmlI 1404 CACGTG CAC^GTG RcaI 1643 TCATGA T^CATGA SacII 777 CCGCGG CCGC^GG SalI 79 GTCGAC G^TCGAC ScaI 575 AGTACT AGT^ACT SgrAI 351 CGCCGGCG C[GA]^CCGG[CT]G SmlI 697 CTCAAG C^T[CT][GA]AG SphI 1658 GCATGC GCATG^C StuI 165 AGGCCT AGG^CCT XcmI 1298 CCATGTACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 79 GTCGAC GT^[AC][GT]AC 1108 GTCTAC AcyI 217 GGCGCC G[GA]^CG[CT]C 349 GGCGCC AflIII 938 ACACGT A^C[GA][CT]GT 1040 ACATGT ApaI 310 GGGCCC GGGCC^C 530 GGGCCC BanI 217 GGCGCC G^G[CT][GA]CC 349 GGCGCC BbeI 217 GGCGCC GGCGC^C 349 GGCGCC BbsI 711 GTCTTC GAAGAC(2/6) 849 GTCTTC BcnI 108 CCGGG CC^[GC]GG 1480 CCGGG BfaI 307 CTAG C^TAG 1189 CTAG BpuAI 711 GTCTTC GAAGAC(2/6) 849 GTCTTC BsaAI 415 TACGTG [CT]AC^GT[GA] 1404 CACGTG BsaHI 217 GGCGCC G[GA]^CG[CT]C 349 GGCGCC BsgI 101 CTGCAC GTGCAG(16/14) 552 CTGCAC BsmFI 1737 GGGAC GGGAC(10/14) 1515 GTCCC BstYI 635 AGATCC [GA]^GATC[CT] 881 AGATCT DsaI 777 CCGCGG C^C[GA][CT]GG 1727 CCATGG Hin1I 217 GGCGCC G[GA]^CG[CT]C 349 GGCGCC KasI 217 GGCGCC G^GCGCC 349 GGCGCC MaeI 307 CTAG C^TAG 1189 CTAG MflI 635 AGATCC [GA]^GATC[CT] 881 AGATCT NarI 217 GGCGCC GG^CGCC 349 GGCGCC NciI 108 CCGGG CC^[GC]GG 1480 CCGGG PflMI 854 CCAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1727 CCATGGACTGG PvuII 1226 CAGCTG CAG^CTG 1652 CAGCTG SacI 17 GAGCTC GAGCT^C 280 GAGCTC SapI 420 GCTCTTC GCTCTTC(1/4) 874 GCTCTTC SfcI 957 CTATAG C^T[GA][CT]AG 1365 CTACAG SfoI 217 GGCGCC GGC^GCC 349 GGCGCC Van91I 854 CCAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1727 CCATGGACTGG XhoII 635 AGATCC [GA]^GATC[CT] 881 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaII BalI BanII BbvI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NaeI NdeII NgoMIV NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I TspRI TthHB8I