Restriction map for
pFN21AE5732
(Circular) (Six-base) MAPSORT of: pFN21AE5732.seq Check: 873 from: 1 to: 6613
pFN21AE5732 6613 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2564 2564
Size: 6613
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4423 278
Size: 53 83 186 936 2887 2468
Fragments arranged by size:
2887 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2085 3114 3956 1937
Size: 148 1029 842 4594
Fragments arranged by size:
4594 1029 842 148
Acc65I G'GTAC_C
Cuts at: 3934 3934
Size: 6613
AclI AA'CG_TT
Cuts at: 1563 4740 5113 1563
Size: 3177 373 3063
Fragments arranged by size:
3177 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2867 3481 4676 6060 6328 1632
Size: 1235 614 1195 1384 268 1917
Fragments arranged by size:
1917 1384 1235 1195 614 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6416
AflIII A'CryG_T
Cuts at: 1203 2943 3045 5512 6489 1203
Size: 1740 102 2467 977 1327
Fragments arranged by size:
2467 1740 1327 977 102
AgeI A'CCGG_T
Cuts at: 5444 5444
Size: 6613
AhdI GACnn_n'nnGTC
Cuts at: 5342 5342
Size: 6613
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6613
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6613
AlwNI CAG_nnn'CTG
Cuts at: 3058 3095 3504 4356 5928 3058
Size: 37 409 852 1572 3743
Fragments arranged by size:
3743 1572 852 409 37
ApaI G_GGCC'C
Cuts at: 2319 2539 2319
Size: 220 6393
ApaLI G'TGCA_C
Cuts at: 3821 4670 5826 3821
Size: 849 1156 4608
Fragments arranged by size:
4608 1156 849
ApoI r'AATT_y
Cuts at: 1493 3922 4148 4300 6409 1493
Size: 2429 226 152 2109 1697
Fragments arranged by size:
2429 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5167 160
Size: 872 4135 1606
Fragments arranged by size:
4135 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6613
AvaI C'yCGr_G
Cuts at: 1870 1943 3938 1870
Size: 73 1995 4545
Fragments arranged by size:
4545 1995 73
AvrII C'CTAG_G
Cuts at: 2311 2311
Size: 6613
BamHI G'GATC_C
Cuts at: 3943 4310 3943
Size: 367 6246
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2222 2354 3934 5389 618
Size: 325 217 627 435 132 1580 1455 1842
Fragments arranged by size:
1842 1580 1455 627 435 325 217 132
BanII G_rGCy'C
Cuts at: 1391 2026 2289 2319 2539 2922 3291 3695 3932
Size: 635 263 30 220 383 369 404 237
Cuts at: 3932 1391
Size: 4072
Fragments arranged by size:
4072 635 404 383 369 263 237 220
30
BbeI G_GCGC'C
Cuts at: 2226 2358 2226
Size: 132 6481
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2709 2847 928
Size: 971 810 138 4694
Fragments arranged by size:
4694 971 810 138
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4804 4838 1903
Size: 34 2867 34 3678
Fragments arranged by size:
3678 2867 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2708 4505 5715 2708
Size: 1797 1210 3606
Fragments arranged by size:
3606 1797 1210
BclI T'GATC_A
Cuts at: 1548 2409 1548
Size: 861 5752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3788 5224 136
Size: 107 122 71 631 605 2116 1436 1525
Fragments arranged by size:
2116 1525 1436 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2886 6608 2886
Size: 3722 2891
BlpI GC'TnA_GC
Cuts at: 3609 4030 3609
Size: 421 6192
Bme1580I G_kGCm'C
Cuts at: 1790 2238 2319 2539 3825 4674 5830 1790
Size: 448 81 220 1286 849 1156 2573
Fragments arranged by size:
2573 1286 1156 849 448 220 81
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6613
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2061 2830 3748
Size: 416 53 83 399 24 644 769 918
Cuts at: 3748 4344 5302 442
Size: 596 958 1753
Fragments arranged by size:
1753 958 918 769 644 596 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6613
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2045 2810 2045
Size: 765 5848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2000 2290 2303 2525 2605 3236 3773 5273
Size: 761 290 13 222 80 631 537 1500
Cuts at: 5273 1239
Size: 2579
Fragments arranged by size:
2579 1500 761 631 537 290 222 80
13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2687 4738 5603 5901 2687
Size: 2051 865 298 3399
Fragments arranged by size:
3399 2051 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3212 5276 882
Size: 617 1713 2064 2219
Fragments arranged by size:
2219 2064 1713 617
BsaAI yAC'GTr
Cuts at: 493 1204 2422 3411 4404 493
Size: 711 1218 989 993 2702
Fragments arranged by size:
2702 1218 993 989 711
BsaBI GATnn'nnATC
Cuts at: 6607 6607
Size: 6613
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2223 2355 4420 4802
Size: 53 83 186 936 690 132 2065 382
Cuts at: 4802 5437 275
Size: 635 1451
Fragments arranged by size:
2065 1451 936 690 635 382 186 132
83 53
BsaWI w'CCGG_w
Cuts at: 3762 5046 5444 5718 5865 6276 3762
Size: 1284 398 274 147 411 4099
Fragments arranged by size:
4099 1284 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2501 2531 3887 3917 1178
Size: 30 165 30 1098 30 1356 30 3874
Fragments arranged by size:
3874 1356 1098 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2232 2591 2912 3770 3908 3911 3914 1186
Size: 1046 359 321 858 138 3 3 3885
Fragments arranged by size:
3885 1046 858 359 321 138 3 3
BseYI C'CCAG_C
Cuts at: 2570 3037 3058 3472 3523 3616 3655 3791 5816
Size: 467 21 414 51 93 39 136 2025
Cuts at: 5816 6312 2570
Size: 496 2871
Fragments arranged by size:
2871 2025 496 467 414 136 93 51
39 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2091 2542 1163
Size: 573 355 451 5234
Fragments arranged by size:
5234 573 451 355
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3728 4063 4824 5852 1540
Size: 275 186 1727 335 761 1028 2301
Fragments arranged by size:
2301 1727 1028 761 335 275 186
BsiHKAI G_wGCw'C
Cuts at: 2026 2289 2427 2472 2511 3825 4674 4759 5830
Size: 263 138 45 39 1314 849 85 1071
Cuts at: 5830 2026
Size: 2809
Fragments arranged by size:
2809 1314 1071 849 263 138 85 45
39
BsmI GAATG_Cn'
Cuts at: 3438 4124 4217 3438
Size: 686 93 5834
Fragments arranged by size:
5834 686 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 2238 2289 2319 2427 2472 2511
Size: 399 236 212 51 30 108 45 39
Cuts at: 2511 2539 2922 3291 3695 3825 3932 4674 4759
Size: 28 383 369 404 130 107 742 85
Cuts at: 4759 5830 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 404 399 383 369 236
212 130 108 107 85 51 45 39
30 28
BspEI T'CCGG_A
Cuts at: 6276 6276
Size: 6613
BspHI T'CATG_A
Cuts at: 3648 4502 6232 3648
Size: 854 1730 4029
Fragments arranged by size:
4029 1730 854
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2061 2564 3267 3968 844
Size: 1217 503 703 701 3489
Fragments arranged by size:
3489 1217 703 701 503
BsrBI CCG'CTC
Cuts at: 1394 1823 3706 4500 1394
Size: 429 1883 794 3507
Fragments arranged by size:
3507 1883 794 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5108 5282 65
Size: 1183 3860 174 1396
Fragments arranged by size:
3860 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2357 3723 3727 5257 5444 1700
Size: 110 547 1366 4 1530 187 2869
Fragments arranged by size:
2869 1530 1366 547 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6613
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6613
BssSI C'ACGA_G
Cuts at: 2231 4673 5685 2231
Size: 2442 1012 3159
Fragments arranged by size:
3159 2442 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6613
BstBI TT'CG_AA
Cuts at: 5417 5417
Size: 6613
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6613
BstXI CCAn_nnnn'nTGG
Cuts at: 2805 3010 3535 3874 2805
Size: 205 525 339 5544
Fragments arranged by size:
5544 525 339 205
BstYI r'GATC_y
Cuts at: 1972 2640 2886 3943 4310 4695 4712 6164 6250
Size: 668 246 1057 367 385 17 1452 86
Cuts at: 6250 6262 6608 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1057 668 385 367 346 246
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2782 3732 513
Size: 552 1717 950 3394
Fragments arranged by size:
3394 1717 950 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2094 2398 487
Size: 683 56 868 304 4702
Fragments arranged by size:
4702 868 683 304 56
BtsI GCAGTG_nn'
Cuts at: 4129 4923 4943 4129
Size: 794 20 5799
Fragments arranged by size:
5799 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6613
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3397 3432 529
Size: 35 2833 35 3710
Fragments arranged by size:
3710 2833 35 35
DraI TTT'AAA
Cuts at: 3917 3917
Size: 6613
DraIII CAC_nnn'GTG
Cuts at: 3411 3411
Size: 6613
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5620 809
Size: 507 4304 1802
Fragments arranged by size:
4304 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 2391 2742
Size: 54 1425 50 275 18 423 138 351
Cuts at: 2742 3300 3725 3729 3780 3864 4060 4949 6578
Size: 558 425 4 51 84 196 889 1629
Cuts at: 6578 8
Size: 43
Fragments arranged by size:
1629 1425 889 558 425 423 351 275
196 138 84 54 51 50 43 18
4
EagI C'GGCC_G
Cuts at: 1537 1812 3725 4060 1537
Size: 275 1913 335 4090
Fragments arranged by size:
4090 1913 335 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2279 2351 2432 2441 2886 3170 3681 4543
Size: 926 72 81 9 445 284 511 862
Cuts at: 4543 1353
Size: 3423
Fragments arranged by size:
3423 926 862 511 445 284 81 72
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5200 5574 5720 387
Size: 1064 138 3611 374 146 1280
Fragments arranged by size:
3611 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2024 2287 2024
Size: 263 6350
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2000 2290 2303 2525 2605 2867 3236
Size: 393 368 290 13 222 80 262 369
Cuts at: 3236 3481 3773 4676 5273 6060 6328 1239
Size: 245 292 903 597 787 268 1524
Fragments arranged by size:
1524 903 787 597 393 369 368 292
290 268 262 245 222 80 13
EcoO109I rG'GnC_Cy
Cuts at: 2315 2316 2535 2536 2802 3092 3501 2315
Size: 1 219 1 266 290 409 5427
Fragments arranged by size:
5427 409 290 266 219 1 1
EcoRI G'AATT_C
Cuts at: 3922 4300 3922
Size: 378 6235
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2155 2897 2155
Size: 742 5871
FseI GG_CCGG'CC
Cuts at: 3731 3731
Size: 6613
FspI TGC'GCA
Cuts at: 3830 5119 3830
Size: 1289 5324
HaeII r_GCGC'y
Cuts at: 1678 2191 2226 2358 3378 5760 1678
Size: 513 35 132 1020 2382 2531
Fragments arranged by size:
2531 2382 1020 513 132 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3887 3919 5254 5286 5328
Size: 32 135 32 2315 32 1335 32 42
Cuts at: 5328 5360 1373
Size: 32 2626
Fragments arranged by size:
2626 2315 1335 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2086 3957 4203 669
Size: 988 281 148 1871 246 3079
Fragments arranged by size:
3079 1871 988 281 246 148
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6613
HpaI GTT'AAC
Cuts at: 4203 4203
Size: 6613
KasI G'GCGC_C
Cuts at: 2222 2354 2222
Size: 132 6481
KpnI G_GTAC'C
Cuts at: 3938 3938
Size: 6613
MfeI C'AATT_G
Cuts at: 4212 4212
Size: 6613
MluI A'CGCG_T
Cuts at: 6489 6489
Size: 6613
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3736 5727 5911 1305
Size: 2431 1991 184 2007
Fragments arranged by size:
2431 2007 1991 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2255 2393 3302 3782 10
Size: 54 1425 343 423 138 909 480 2841
Fragments arranged by size:
2841 1425 909 480 423 343 138 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3131 3296 3464 3626 3668 3776 3872
Size: 648 1965 165 168 162 42 108 96
Cuts at: 3872 4571 4930 5089 518
Size: 699 359 159 2042
Fragments arranged by size:
2042 1965 699 648 359 168 165 162
159 108 96 42
MspA1I CmG'CkG
Cuts at: 1606 2646 2784 3233 3659 4029 4706 5854 6099
Size: 1040 138 449 426 370 677 1148 245
Cuts at: 6099 1606
Size: 2120
Fragments arranged by size:
2120 1148 1040 677 449 426 370 245
138
NaeI GCC'GGC
Cuts at: 2359 3725 3729 2359
Size: 1366 4 5243
Fragments arranged by size:
5243 1366 4
NarI GG'CG_CC
Cuts at: 2223 2355 2223
Size: 132 6481
NcoI C'CATG_G
Cuts at: 513 1065 3732 513
Size: 552 2667 3394
Fragments arranged by size:
3394 2667 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6613
NgoMIV G'CCGG_C
Cuts at: 2357 3723 3727 2357
Size: 1366 4 5243
Fragments arranged by size:
5243 1366 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6613
NotI GC'GGCC_GC
Cuts at: 4060 4060
Size: 6613
NspI r_CATG'y
Cuts at: 1132 1229 3049 3346 3667 3775 3829 3971 5516
Size: 97 1820 297 321 108 54 142 1545
Cuts at: 5516 1132
Size: 2229
Fragments arranged by size:
2229 1820 1545 321 297 142 108 97
54
PasI CC'CwG_GG
Cuts at: 1348 2293 2463 2633 2798 2899 3603 1348
Size: 945 170 170 165 101 704 4358
Fragments arranged by size:
4358 945 704 170 170 165 101
PciI A'CATG_T
Cuts at: 3045 5512 3045
Size: 2467 4146
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2865 3738 1108
Size: 1757 873 3983
Fragments arranged by size:
3983 1757 873
PfoI T'CCnGG_A
Cuts at: 2280 2280
Size: 6613
PmeI GTTT'AAAC
Cuts at: 3917 3917
Size: 6613
PmlI CAC'GTG
Cuts at: 3411 3411
Size: 6613
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4682 4714 6195 6227 4682
Size: 32 1481 32 5068
Fragments arranged by size:
5068 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6613
PsiI TTA'TAA
Cuts at: 4183 4183
Size: 6613
PspOMI G'GGCC_C
Cuts at: 2315 2535 2315
Size: 220 6393
PstI C_TGCA'G
Cuts at: 830 3965 830
Size: 3135 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6613
PvuII CAG'CTG
Cuts at: 3233 3659 3233
Size: 426 6187
SacI G_AGCT'C
Cuts at: 2026 2289 2026
Size: 263 6350
SacII CC_GC'GG
Cuts at: 2785 2785
Size: 6613
SalI G'TCGA_C
Cuts at: 1936 2084 3955 1936
Size: 148 1871 4594
Fragments arranged by size:
4594 1871 148
SapI GCTCTTCn'nnn_
Cuts at: 2432 2886 2432
Size: 454 6159
SbfI CC_TGCA'GG
Cuts at: 3965 3965
Size: 6613
ScaI AGT'ACT
Cuts at: 2582 4861 6573 2582
Size: 2279 1712 2622
Fragments arranged by size:
2622 2279 1712
SfcI C'TryA_G
Cuts at: 826 1045 2962 3370 3961 5096 5777 5968 826
Size: 219 1917 408 591 1135 681 191 1471
Fragments arranged by size:
1917 1471 1135 681 591 408 219 191
SfoI GGC'GCC
Cuts at: 2224 2356 2224
Size: 132 6481
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6613
SgrAI Cr'CCGG_yG
Cuts at: 1700 2357 1700
Size: 657 5956
SmaI CCC'GGG
Cuts at: 1872 3940 1872
Size: 2068 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2702 4717 5618 5880 820
Size: 197 926 759 2015 901 262 1553
Fragments arranged by size:
2015 1553 926 901 759 262 197
SnaBI TAC'GTA
Cuts at: 493 4404 493
Size: 3911 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6613
SphI G_CATG'C
Cuts at: 1132 3667 3971 1132
Size: 2535 304 3774
Fragments arranged by size:
3774 2535 304
SspI AAT'ATT
Cuts at: 5 52 4537 5
Size: 47 4485 2081
Fragments arranged by size:
4485 2081 47
StuI AGG'CCT
Cuts at: 1517 2172 1517
Size: 655 5958
StyI C'CwwG_G
Cuts at: 513 1065 2311 2520 2608 2859 3732 4052 513
Size: 552 1246 209 88 251 873 320 3074
Fragments arranged by size:
3074 1246 873 552 320 251 209 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2891 4640 4657 4810 4995 5440 325
Size: 1255 1311 1749 17 153 185 445 1498
Fragments arranged by size:
1749 1498 1311 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2580 3345 4859
Size: 274 80 33 51 1443 603 765 1514
Cuts at: 4859 6571 96
Size: 1712 138
Fragments arranged by size:
1712 1514 1443 765 603 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2501 2533 6573 6605 2501
Size: 32 4040 32 2509
Fragments arranged by size:
4040 2509 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6613
XbaI T'CTAG_A
Cuts at: 3949 3949
Size: 6613
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3310 3310
Size: 6613
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6613
XmaI C'CCGG_G
Cuts at: 1870 3938 1870
Size: 2068 4545
XmnI GAAnn'nnTTC
Cuts at: 4742 4742
Size: 6613
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4421 276
Size: 53 83 186 936 2887 2468
Fragments arranged by size:
2887 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I EcoNI EcoRV FspAI NruI NsiI
PacI PpuMI PspXI PsrI RsrII SanDI SexAI SfiI
SrfI SwaI