(Linear) (Six-base) MAPSORT of: KIEE5732.seq Check: 4099 from: 1 to: 1908 KIEE5732.orf With 182 enzymes: SgfI * December 5, 2014 13:08 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 559 1908 Size: 559 1349 AccI GT'mk_AC Cuts at: 0 80 1109 1908 Size: 80 1029 799 Fragments arranged by size: 1029 799 80 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 862 1476 1908 Size: 862 614 432 Fragments arranged by size: 862 614 432 AflIII A'CryG_T Cuts at: 0 938 1040 1908 Size: 938 102 868 Fragments arranged by size: 938 868 102 AlwNI CAG_nnn'CTG Cuts at: 0 1053 1090 1499 1908 Size: 1053 37 409 409 Fragments arranged by size: 1053 409 409 37 ApaI G_GGCC'C Cuts at: 0 314 534 1908 Size: 314 220 1374 Fragments arranged by size: 1374 314 220 ApaLI G'TGCA_C Cuts at: 0 1816 1908 Size: 1816 92 AvrII C'CTAG_G Cuts at: 0 306 1908 Size: 306 1602 BanI G'GyrC_C Cuts at: 0 217 349 1908 Size: 217 132 1559 Fragments arranged by size: 1559 217 132 BanII G_rGCy'C Cuts at: 0 21 284 314 534 917 1286 1690 1908 Size: 21 263 30 220 383 369 404 218 Fragments arranged by size: 404 383 369 263 220 218 30 21 BbeI G_GCGC'C Cuts at: 0 221 353 1908 Size: 221 132 1555 Fragments arranged by size: 1555 221 132 BbsI GAAGACnn'nnnn_ Cuts at: 0 704 842 1908 Size: 704 138 1066 Fragments arranged by size: 1066 704 138 BciVI GTATCCnnnnn_n' Cuts at: 0 703 1908 Size: 703 1205 BclI T'GATC_A Cuts at: 0 404 1908 Size: 404 1504 BglI GCCn_nnn'nGGC Cuts at: 0 1783 1908 Size: 1783 125 BglII A'GATC_T Cuts at: 0 881 1908 Size: 881 1027 BlpI GC'TnA_GC Cuts at: 0 1604 1908 Size: 1604 304 Bme1580I G_kGCm'C Cuts at: 0 233 314 534 1820 1908 Size: 233 81 220 1286 88 Fragments arranged by size: 1286 233 220 88 81 BmrI ACTGGGnnnn_n' Cuts at: 0 56 825 1743 1908 Size: 56 769 918 165 Fragments arranged by size: 918 769 165 56 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 40 805 1908 Size: 40 765 1103 Fragments arranged by size: 1103 765 40 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 285 298 520 600 1231 1768 1908 Size: 285 13 222 80 631 537 140 Fragments arranged by size: 631 537 285 222 140 80 13 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 682 1908 Size: 682 1226 BsaI GGTCTCn'nnnn_ Cuts at: 0 1207 1908 Size: 1207 701 BsaAI yAC'GTr Cuts at: 0 417 1406 1908 Size: 417 989 502 Fragments arranged by size: 989 502 417 BsaHI Gr'CG_yC Cuts at: 0 218 350 1908 Size: 218 132 1558 Fragments arranged by size: 1558 218 132 BsaWI w'CCGG_w Cuts at: 0 1757 1908 Size: 1757 151 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 496 526 1882 1908 Size: 496 30 1356 26 Fragments arranged by size: 1356 496 30 26 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 227 586 907 1765 1903 1906 1908 Size: 227 359 321 858 138 3 2 Fragments arranged by size: 858 359 321 227 138 3 2 BseYI C'CCAG_C Cuts at: 0 565 1032 1053 1467 1518 1611 1650 1786 Size: 565 467 21 414 51 93 39 136 Cuts at: 1786 1908 Size: 122 Fragments arranged by size: 565 467 414 136 122 93 51 39 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 86 537 1908 Size: 86 451 1371 Fragments arranged by size: 1371 451 86 BsiEI CG_ry'CG Cuts at: 0 1723 1908 Size: 1723 185 BsiHKAI G_wGCw'C Cuts at: 0 21 284 422 467 506 1820 1908 Size: 21 263 138 45 39 1314 88 Fragments arranged by size: 1314 263 138 88 45 39 21 BsmI GAATG_Cn' Cuts at: 0 1433 1908 Size: 1433 475 Bsp1286I G_dGCh'C Cuts at: 0 21 233 284 314 422 467 506 534 Size: 21 212 51 30 108 45 39 28 Cuts at: 534 917 1286 1690 1820 1908 Size: 383 369 404 130 88 Fragments arranged by size: 404 383 369 212 130 108 88 51 45 39 30 28 21 BspHI T'CATG_A Cuts at: 0 1643 1908 Size: 1643 265 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 56 559 1262 1908 Size: 56 503 703 646 Fragments arranged by size: 703 646 503 56 BsrBI CCG'CTC Cuts at: 0 1701 1908 Size: 1701 207 BsrFI r'CCGG_y Cuts at: 0 352 1718 1722 1908 Size: 352 1366 4 186 Fragments arranged by size: 1366 352 186 4 BssSI C'ACGA_G Cuts at: 0 226 1908 Size: 226 1682 BstXI CCAn_nnnn'nTGG Cuts at: 0 800 1005 1530 1869 1908 Size: 800 205 525 339 39 Fragments arranged by size: 800 525 339 205 39 BstYI r'GATC_y Cuts at: 0 635 881 1908 Size: 635 246 1027 Fragments arranged by size: 1027 635 246 BtgI C'CryG_G Cuts at: 0 777 1727 1908 Size: 777 950 181 Fragments arranged by size: 950 777 181 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 89 393 1908 Size: 89 304 1515 Fragments arranged by size: 1515 304 89 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1392 1427 1908 Size: 1392 35 481 Fragments arranged by size: 1392 481 35 DraIII CAC_nnn'GTG Cuts at: 0 1406 1908 Size: 1406 502 EaeI y'GGCC_r Cuts at: 0 248 386 737 1295 1720 1724 1775 1859 Size: 248 138 351 558 425 4 51 84 Cuts at: 1859 1908 Size: 49 Fragments arranged by size: 558 425 351 248 138 84 51 49 4 EagI C'GGCC_G Cuts at: 0 1720 1908 Size: 1720 188 EarI CTCTTCn'nnn_ Cuts at: 0 274 346 427 436 881 1165 1676 1908 Size: 274 72 81 9 445 284 511 232 Fragments arranged by size: 511 445 284 274 232 81 72 9 EcoICRI GAG'CTC Cuts at: 0 19 282 1908 Size: 19 263 1626 Fragments arranged by size: 1626 263 19 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 285 298 520 600 862 1231 1476 1768 Size: 285 13 222 80 262 369 245 292 Cuts at: 1768 1908 Size: 140 Fragments arranged by size: 369 292 285 262 245 222 140 80 13 EcoO109I rG'GnC_Cy Cuts at: 0 310 311 530 531 797 1087 1496 1908 Size: 310 1 219 1 266 290 409 412 Fragments arranged by size: 412 409 310 290 266 219 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 150 892 1908 Size: 150 742 1016 Fragments arranged by size: 1016 742 150 FseI GG_CCGG'CC Cuts at: 0 1726 1908 Size: 1726 182 FspI TGC'GCA Cuts at: 0 1825 1908 Size: 1825 83 HaeII r_GCGC'y Cuts at: 0 186 221 353 1373 1908 Size: 186 35 132 1020 535 Fragments arranged by size: 1020 535 186 132 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1882 1908 Size: 1882 26 HincII GTy'rAC Cuts at: 0 81 1908 Size: 81 1827 KasI G'GCGC_C Cuts at: 0 217 349 1908 Size: 217 132 1559 Fragments arranged by size: 1559 217 132 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1731 1908 Size: 1731 177 MscI TGG'CCA Cuts at: 0 250 388 1297 1777 1908 Size: 250 138 909 480 131 Fragments arranged by size: 909 480 250 138 131 MslI CAynn'nnrTG Cuts at: 0 1126 1291 1459 1621 1663 1771 1867 1908 Size: 1126 165 168 162 42 108 96 41 Fragments arranged by size: 1126 168 165 162 108 96 42 41 MspA1I CmG'CkG Cuts at: 0 641 779 1228 1654 1908 Size: 641 138 449 426 254 Fragments arranged by size: 641 449 426 254 138 NaeI GCC'GGC Cuts at: 0 354 1720 1724 1908 Size: 354 1366 4 184 Fragments arranged by size: 1366 354 184 4 NarI GG'CG_CC Cuts at: 0 218 350 1908 Size: 218 132 1558 Fragments arranged by size: 1558 218 132 NcoI C'CATG_G Cuts at: 0 1727 1908 Size: 1727 181 NgoMIV G'CCGG_C Cuts at: 0 352 1718 1722 1908 Size: 352 1366 4 186 Fragments arranged by size: 1366 352 186 4 NspI r_CATG'y Cuts at: 0 1044 1341 1662 1770 1824 1908 Size: 1044 297 321 108 54 84 Fragments arranged by size: 1044 321 297 108 84 54 PasI CC'CwG_GG Cuts at: 0 288 458 628 793 894 1598 1908 Size: 288 170 170 165 101 704 310 Fragments arranged by size: 704 310 288 170 170 165 101 PciI A'CATG_T Cuts at: 0 1040 1908 Size: 1040 868 PflMI CCAn_nnn'nTGG Cuts at: 0 860 1733 1908 Size: 860 873 175 Fragments arranged by size: 873 860 175 PfoI T'CCnGG_A Cuts at: 0 275 1908 Size: 275 1633 PmlI CAC'GTG Cuts at: 0 1406 1908 Size: 1406 502 PspOMI G'GGCC_C Cuts at: 0 310 530 1908 Size: 310 220 1378 Fragments arranged by size: 1378 310 220 PvuII CAG'CTG Cuts at: 0 1228 1654 1908 Size: 1228 426 254 Fragments arranged by size: 1228 426 254 SacI G_AGCT'C Cuts at: 0 21 284 1908 Size: 21 263 1624 Fragments arranged by size: 1624 263 21 SacII CC_GC'GG Cuts at: 0 780 1908 Size: 780 1128 SalI G'TCGA_C Cuts at: 0 79 1908 Size: 79 1829 SapI GCTCTTCn'nnn_ Cuts at: 0 427 881 1908 Size: 427 454 1027 Fragments arranged by size: 1027 454 427 ScaI AGT'ACT Cuts at: 0 577 1908 Size: 577 1331 SfcI C'TryA_G Cuts at: 0 957 1365 1908 Size: 957 408 543 Fragments arranged by size: 957 543 408 SfoI GGC'GCC Cuts at: 0 219 351 1908 Size: 219 132 1557 Fragments arranged by size: 1557 219 132 SgrAI Cr'CCGG_yG Cuts at: 0 352 1908 Size: 352 1556 SmlI C'TyrA_G Cuts at: 0 697 1908 Size: 697 1211 SphI G_CATG'C Cuts at: 0 1662 1908 Size: 1662 246 StuI AGG'CCT Cuts at: 0 167 1908 Size: 167 1741 StyI C'CwwG_G Cuts at: 0 306 515 603 854 1727 1908 Size: 306 209 88 251 873 181 Fragments arranged by size: 873 306 251 209 181 88 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 886 1908 Size: 886 1022 TatI w'GTAC_w Cuts at: 0 575 1340 1908 Size: 575 765 568 Fragments arranged by size: 765 575 568 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 496 528 1908 Size: 496 32 1380 Fragments arranged by size: 1380 496 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1305 1908 Size: 1305 603 Enzymes that do cut: AarI AccI AcuI AflIII AlwNI ApaI ApaLI AvrII BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstXI BstYI BtgI BtgZI CspCI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoO109I FalI FseI FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmlI PspOMI PvuII SacI SacII SalI SapI ScaI SfcI SfoI SgrAI SmlI SphI StuI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvaI BaeI BamHI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaBI BsiWI BsmBI BspEI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI DraI DrdI EciI EcoNI EcoRI EcoRV FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII SanDI SbfI SexAI SfiI SgfI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI