################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BbrPI BcgI BciVI BclI BfrI BlnI BsaAI BsaBI BseAI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrDI BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MamI MfeI MluI MroI MslI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspI SwaI XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AgeI 2843 ACCGGT A^CCGGT AspI 2365 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1851 GGATCC G^GATCC BglII 553 AGATCT A^GATCT BlpI 2919 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2919 GCTGAGC GC^T[ACGT]AGC BsaWI 2843 ACCGGT [AT]^CCGG[AT] BsgI 398 CTGCAC GTGCAG(16/14) BsmBI 356 GAGACG CGTCTC(1/5) BssHII 20 GCGCGC G^CGCGC BstAPI 3057 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2591 GGTCACC G^GT[ACGT]ACC BstPI 2591 GGTCACC G^GT[ACGT]ACC BstXI 290 CCAAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 2919 GCTGAGC GC^T[ACGT]AGC DrdI 2470 GACTCAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 2591 GGTCACC G^GT[ACGT]ACC HincII 563 GTCAAC GT[CT]^[GA]AC HindII 563 GTCAAC GT[CT]^[GA]AC MseI 578 TTAA T^TAA NaeI 1262 GCCGGC GCC^GGC NgoMIV 1262 GCCGGC G^CCGGC PflFI 2365 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1747 CCAACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2843 ACCGGT A^CCGGT PshAI 319 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 678 CCTGCAGG CCTGCA^GG SmaI 791 CCCGGG CCC^GGG Sse8387I 678 CCTGCAGG CCTGCA^GG TaqI 2604 TCGA T^CGA TfiI 2971 GATTC G^A[AT]TC Tru9I 578 TTAA T^TAA Tsp509I 2977 AATT ^AATT Tth111I 2365 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 2604 TCGA T^CGA Van91I 1747 CCAACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 791 CCCGGG C^CCGGG XmaI 791 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### ApaI 1210 GGGCCC GGGCC^C 1458 GGGCCC BbeI 536 GGCGCC GGCGC^C 2236 GGCGCC BfaI 802 CTAG C^TAG 1882 CTAG Bsp1407I 637 TGTACA T^GTACA 2711 TGTACA BsrBI 2556 CCGCTC CCGCTC(-3/-3) 1555 GAGCGG BsrGI 637 TGTACA T^GTACA 2711 TGTACA BsrI 2162 ACTGG ACTGG(1/-1) 219 CCAGT KasI 536 GGCGCC G^GCGCC 2236 GGCGCC MaeI 802 CTAG C^TAG 1882 CTAG MaeIII 1734 GTCAC ^GT[ACGT]AC 2592 GTCAC NarI 536 GGCGCC GG^CGCC 2236 GGCGCC NcoI 1381 CCATGG C^CATGG 3023 CCATGG SfoI 536 GGCGCC GGC^GCC 2236 GGCGCC SspBI 637 TGTACA T^GTACA 2711 TGTACA Tsp45I 1734 GTCAC ^GT[GC]AC 2592 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TseI TspRI XhoII