(Circular) (Six-base) MAPSORT of: pFN21AE2683.seq Check: 205 from: 1 to: 7774 pFN21AE2683 7774 bp With 182 enzymes: SgfI * March 22, 2017 12:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2180 4014 2180 Size: 1834 5940 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5584 278 Size: 53 83 186 936 4048 2468 Fragments arranged by size: 4048 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5117 1937 Size: 3180 4594 Acc65I G'GTAC_C Cuts at: 5095 5095 Size: 7774 AclI AA'CG_TT Cuts at: 1563 5901 6274 1563 Size: 4338 373 3063 Fragments arranged by size: 4338 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2337 4603 4778 5837 7221 7489 1632 Size: 705 2266 175 1059 1384 268 1917 Fragments arranged by size: 2266 1917 1384 1059 705 268 175 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7577 AflIII A'CryG_T Cuts at: 1203 6673 7650 1203 Size: 5470 977 1327 Fragments arranged by size: 5470 1327 977 AgeI A'CCGG_T Cuts at: 4848 6605 4848 Size: 1757 6017 AhdI GACnn_n'nnGTC Cuts at: 6503 6503 Size: 7774 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7774 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2127 2695 1184 Size: 943 568 6263 Fragments arranged by size: 6263 943 568 AlwNI CAG_nnn'CTG Cuts at: 2213 2447 3953 4230 5068 5517 7089 2213 Size: 234 1506 277 838 449 1572 2898 Fragments arranged by size: 2898 1572 1506 838 449 277 234 ApaI G_GGCC'C Cuts at: 3219 3467 3219 Size: 248 7526 ApaLI G'TGCA_C Cuts at: 5831 6987 5831 Size: 1156 6618 ApoI r'AATT_y Cuts at: 1493 5083 5309 5461 7570 1493 Size: 3590 226 152 2109 1697 Fragments arranged by size: 3590 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6328 160 Size: 872 5296 1606 Fragments arranged by size: 5296 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7774 AvaI C'yCGr_G Cuts at: 1870 1943 2767 2796 4749 5099 1870 Size: 73 824 29 1953 350 4545 Fragments arranged by size: 4545 1953 824 350 73 29 BamHI G'GATC_C Cuts at: 3856 5104 5471 3856 Size: 1248 367 6159 Fragments arranged by size: 6159 1248 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2193 2541 2831 2994 3720 Size: 325 217 627 406 348 290 163 726 Cuts at: 3720 4181 4241 5095 6550 618 Size: 461 60 854 1455 1842 Fragments arranged by size: 1842 1455 854 726 627 461 406 348 325 290 217 163 60 BanII G_rGCy'C Cuts at: 1391 2046 2250 2292 2463 2774 3219 3419 3467 Size: 655 204 42 171 311 445 200 48 Cuts at: 3467 3510 3647 3798 3996 4361 4523 4748 4914 Size: 43 137 151 198 365 162 225 166 Cuts at: 4914 4941 5093 1391 Size: 27 152 4072 Fragments arranged by size: 4072 655 445 365 311 225 204 200 198 171 166 162 152 151 137 48 43 42 27 BbeI G_GCGC'C Cuts at: 2545 4245 2545 Size: 1700 6074 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2856 3306 4227 4344 4389 928 Size: 971 957 450 921 117 45 4313 Fragments arranged by size: 4313 971 957 921 450 117 45 BbvCI CC'TCA_GC Cuts at: 2380 4130 4194 2380 Size: 1750 64 5960 Fragments arranged by size: 5960 1750 64 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5965 5999 1903 Size: 34 4028 34 3678 Fragments arranged by size: 4028 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5666 6876 5666 Size: 1210 6564 BclI T'GATC_A Cuts at: 1548 1548 Size: 7774 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2282 2550 3014 Size: 107 122 71 631 605 610 268 464 Cuts at: 3014 4037 4190 6385 136 Size: 1023 153 2195 1525 Fragments arranged by size: 2195 1525 1023 631 610 605 464 268 153 122 107 71 BglII A'GATC_T Cuts at: 2558 7769 2558 Size: 5211 2563 BlpI GC'TnA_GC Cuts at: 4925 5191 4925 Size: 266 7508 Bme1580I G_kGCm'C Cuts at: 1790 2198 2836 3219 3428 3467 4186 5835 6991 Size: 408 638 383 209 39 719 1649 1156 Cuts at: 6991 1790 Size: 2573 Fragments arranged by size: 2573 1649 1156 719 638 408 383 209 39 BmgBI CAC'GTC Cuts at: 1318 2326 3055 1318 Size: 1008 729 6037 Fragments arranged by size: 6037 1008 729 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5505 6463 442 Size: 416 53 83 399 24 4088 958 1753 Fragments arranged by size: 4088 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7774 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2065 2449 3946 2065 Size: 384 1497 5893 Fragments arranged by size: 5893 1497 384 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2699 2769 3266 3431 3659 4337 4349 4717 Size: 1460 70 497 165 228 678 12 368 Cuts at: 4717 6434 1239 Size: 1717 2579 Fragments arranged by size: 2579 1717 1460 678 497 368 228 165 70 12 Bpu10I CC'TnA_GC Cuts at: 2380 2931 3456 4130 4194 2380 Size: 551 525 674 64 5960 Fragments arranged by size: 5960 674 551 525 64 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3033 3920 4181 4549 5899 6764 7062 3033 Size: 887 261 368 1350 865 298 3745 Fragments arranged by size: 3745 1350 887 865 368 298 261 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4068 4266 4313 6437 882 Size: 617 2569 198 47 2124 2219 Fragments arranged by size: 2569 2219 2124 617 198 47 BsaAI yAC'GTr Cuts at: 493 1204 5565 493 Size: 711 4361 2702 Fragments arranged by size: 4361 2702 711 BsaBI GATnn'nnATC Cuts at: 7768 7768 Size: 7774 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2542 4110 4242 5581 Size: 53 83 186 936 1009 1568 132 1339 Cuts at: 5581 5963 6598 275 Size: 382 635 1451 Fragments arranged by size: 1568 1451 1339 1009 936 635 382 186 132 83 53 BsaWI w'CCGG_w Cuts at: 4848 6207 6605 6879 7026 7437 4848 Size: 1359 398 274 147 411 5185 Fragments arranged by size: 5185 1359 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2174 2204 2697 2727 3338 Size: 30 165 30 771 30 493 30 611 Cuts at: 3338 3368 4331 4361 4730 4760 4898 4928 1178 Size: 30 963 30 369 30 138 30 4024 Fragments arranged by size: 4024 963 771 611 493 369 165 138 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2038 2114 2471 3194 3308 3329 3332 3356 Size: 852 76 357 723 114 21 3 24 Cuts at: 3356 3359 3362 3365 3866 4346 4439 4460 4463 Size: 3 3 3 501 480 93 21 3 Cuts at: 4463 4466 4469 4805 1186 Size: 3 3 336 4155 Fragments arranged by size: 4155 852 723 501 480 357 336 114 93 76 24 21 21 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2139 2344 2411 2440 2775 2964 3397 3460 3568 Size: 205 67 29 335 189 433 63 108 Cuts at: 3568 3683 4023 4173 5058 6977 7473 2139 Size: 115 340 150 885 1919 496 2440 Fragments arranged by size: 2440 1919 885 496 433 340 335 205 189 150 115 108 67 63 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2388 1163 Size: 573 652 6549 Fragments arranged by size: 6549 652 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5224 5985 7013 1540 Size: 275 186 3223 761 1028 2301 Fragments arranged by size: 3223 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 2250 2292 2463 3060 3212 3419 3520 3798 Size: 204 42 171 597 152 207 101 278 Cuts at: 3798 3996 4098 4748 4863 5835 5920 6991 2046 Size: 198 102 650 115 972 85 1071 2829 Fragments arranged by size: 2829 1071 972 650 597 278 207 204 198 171 152 115 102 101 85 42 BsmI GAATG_Cn' Cuts at: 5285 5378 5285 Size: 93 7681 BsmBI CGTCTCn'nnnn_ Cuts at: 2355 2355 Size: 7774 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2198 2250 2292 2463 2774 2836 Size: 399 256 152 52 42 171 311 62 Cuts at: 2836 3060 3212 3219 3419 3428 3467 3510 3520 Size: 224 152 7 200 9 39 43 10 Cuts at: 3520 3647 3798 3996 4098 4186 4361 4523 4748 Size: 127 151 198 102 88 175 162 225 Cuts at: 4748 4863 4914 4941 5093 5835 5920 6991 1391 Size: 115 51 27 152 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 311 256 225 224 200 198 175 171 162 152 152 152 151 127 115 102 88 85 62 52 51 43 42 39 27 10 9 7 BspEI T'CCGG_A Cuts at: 7437 7437 Size: 7774 BspHI T'CATG_A Cuts at: 5663 7393 5663 Size: 1730 6044 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2180 2959 4014 5129 844 Size: 1336 779 1055 1115 3489 Fragments arranged by size: 3489 1336 1115 1055 779 BsrBI CCG'CTC Cuts at: 1394 1823 3562 4563 5661 1394 Size: 429 1739 1001 1098 3507 Fragments arranged by size: 3507 1739 1098 1001 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6269 6443 65 Size: 1183 5021 174 1396 Fragments arranged by size: 5021 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3267 4539 4848 6418 6605 1700 Size: 110 1457 1272 309 1570 187 2869 Fragments arranged by size: 2869 1570 1457 1272 309 187 110 BsrGI T'GTAC_A Cuts at: 96 2642 4716 96 Size: 2546 2074 3154 Fragments arranged by size: 3154 2546 2074 BssHII G'CGCG_C Cuts at: 1925 2025 1925 Size: 100 7674 BssSI C'ACGA_G Cuts at: 5834 6846 5834 Size: 1012 6762 BstAPI GCAn_nnn'nTGC Cuts at: 794 5068 794 Size: 4274 3500 BstBI TT'CG_AA Cuts at: 6578 6578 Size: 7774 BstEII G'GTnAC_C Cuts at: 1184 4596 1184 Size: 3412 4362 BstXI CCAn_nnnn'nTGG Cuts at: 2302 2302 Size: 7774 BstYI r'GATC_y Cuts at: 1972 2558 2975 3627 3856 4281 5104 5471 5856 Size: 586 417 652 229 425 823 367 385 Cuts at: 5856 5873 7325 7411 7423 7769 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 823 652 586 425 417 385 367 346 229 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2448 3921 4895 2448 Size: 1473 974 5327 Fragments arranged by size: 5327 1473 974 BtgI C'CryG_G Cuts at: 513 1065 2004 3386 3579 5028 513 Size: 552 939 1382 193 1449 3259 Fragments arranged by size: 3259 1449 1382 939 552 193 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2876 487 Size: 683 56 1650 5385 Fragments arranged by size: 5385 1650 683 56 BtsI GCAGTG_nn' Cuts at: 2195 2465 3236 4504 5290 6084 6104 2195 Size: 270 771 1268 786 794 20 3865 Fragments arranged by size: 3865 1268 794 786 771 270 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7774 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2158 2193 529 Size: 35 1594 35 6110 Fragments arranged by size: 6110 1594 35 35 DraI TTT'AAA Cuts at: 5078 5078 Size: 7774 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4481 6781 809 Size: 507 3165 2300 1802 Fragments arranged by size: 3165 2300 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2166 2487 3087 Size: 54 1425 50 275 18 336 321 600 Cuts at: 3087 3595 4042 5221 6110 7739 8 Size: 508 447 1179 889 1629 43 Fragments arranged by size: 1629 1425 1179 889 600 508 447 336 321 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5221 1537 Size: 275 3409 4090 Fragments arranged by size: 4090 3409 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2034 2255 3306 3318 3761 4425 4464 4582 Size: 681 221 1051 12 443 664 39 118 Cuts at: 4582 4754 4983 5704 1353 Size: 172 229 721 3423 Fragments arranged by size: 3423 1051 721 681 664 443 229 221 172 118 39 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2603 3254 6361 6735 6881 387 Size: 1064 138 1014 651 3107 374 146 1280 Fragments arranged by size: 3107 1280 1064 1014 651 374 146 138 EcoICRI GAG'CTC Cuts at: 2044 2248 2290 2461 3417 3796 3994 4746 2044 Size: 204 42 171 956 379 198 752 5072 Fragments arranged by size: 5072 956 752 379 204 198 171 42 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2337 2699 2769 3266 3431 3659 4337 Size: 393 705 362 70 497 165 228 678 Cuts at: 4337 4349 4603 4717 4778 5837 6434 7221 7489 Size: 12 254 114 61 1059 597 787 268 Cuts at: 7489 1239 Size: 1524 Fragments arranged by size: 1524 1059 787 705 678 597 497 393 362 268 254 228 165 114 70 61 12 EcoNI CCTnn'n_nnAGG Cuts at: 4331 4421 5004 4331 Size: 90 583 7101 Fragments arranged by size: 7101 583 90 EcoO109I rG'GnC_Cy Cuts at: 2275 3132 3464 3620 3679 3925 4134 4204 4993 Size: 857 332 156 59 246 209 70 789 Cuts at: 4993 2275 Size: 5056 Fragments arranged by size: 5056 857 789 332 246 209 156 70 59 EcoRI G'AATT_C Cuts at: 5083 5461 5083 Size: 378 7396 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3787 4072 4783 4852 5012 3787 Size: 285 711 69 160 6549 Fragments arranged by size: 6549 711 285 160 69 FspI TGC'GCA Cuts at: 6280 6280 Size: 7774 HaeII r_GCGC'y Cuts at: 1678 2545 4245 4768 4890 6921 1678 Size: 867 1700 523 122 2031 2531 Fragments arranged by size: 2531 2031 1700 867 523 122 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2147 2179 2354 2386 2447 Size: 32 135 32 575 32 175 32 61 Cuts at: 2447 2479 2611 2643 3612 3644 3983 4015 4331 Size: 32 132 32 969 32 339 32 316 Cuts at: 4331 4363 4730 4762 6415 6447 6489 6521 1373 Size: 32 367 32 1653 32 42 32 2626 Fragments arranged by size: 2626 1653 969 575 367 339 316 175 135 132 61 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2570 5118 5364 669 Size: 988 281 632 2548 246 3079 Fragments arranged by size: 3079 2548 988 632 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7774 HpaI GTT'AAC Cuts at: 5364 5364 Size: 7774 KasI G'GCGC_C Cuts at: 2541 4241 2541 Size: 1700 6074 KpnI G_GTAC'C Cuts at: 5099 5099 Size: 7774 MfeI C'AATT_G Cuts at: 5373 5373 Size: 7774 MluI A'CGCG_T Cuts at: 7650 7650 Size: 7774 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4123 6888 7072 1305 Size: 2818 2765 184 2007 Fragments arranged by size: 2818 2765 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2489 3089 3597 4044 10 Size: 54 1425 343 657 600 508 447 3740 Fragments arranged by size: 3740 1425 657 600 508 447 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5732 6091 6250 518 Size: 648 4566 359 159 2042 Fragments arranged by size: 4566 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2143 3493 3687 4401 4924 5190 5867 7015 Size: 537 1350 194 714 523 266 677 1148 Cuts at: 7015 7260 1606 Size: 245 2120 Fragments arranged by size: 2120 1350 1148 714 677 537 523 266 245 194 NaeI GCC'GGC Cuts at: 3269 3269 Size: 7774 NarI GG'CG_CC Cuts at: 2542 4242 2542 Size: 1700 6074 NcoI C'CATG_G Cuts at: 513 1065 2004 3386 5028 513 Size: 552 939 1382 1642 3259 Fragments arranged by size: 3259 1642 1382 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7774 NgoMIV G'CCGG_C Cuts at: 3267 3267 Size: 7774 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7774 NotI GC'GGCC_GC Cuts at: 5221 5221 Size: 7774 NspI r_CATG'y Cuts at: 1132 1229 2857 2887 4016 5075 5132 6677 1132 Size: 97 1628 30 1129 1059 57 1545 2229 Fragments arranged by size: 2229 1628 1545 1129 1059 97 57 30 PasI CC'CwG_GG Cuts at: 1348 3947 1348 Size: 2599 5175 PciI A'CATG_T Cuts at: 6673 6673 Size: 7774 PflMI CCAn_nnn'nTGG Cuts at: 1108 3758 1108 Size: 2650 5124 PmeI GTTT'AAAC Cuts at: 5078 5078 Size: 7774 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2118 2150 5843 5875 7356 7388 2118 Size: 32 3693 32 1481 32 2504 Fragments arranged by size: 3693 2504 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3620 3679 4134 3620 Size: 59 455 7260 Fragments arranged by size: 7260 455 59 PshAI GACnn'nnGTC Cuts at: 1613 2328 1613 Size: 715 7059 PsiI TTA'TAA Cuts at: 5344 5344 Size: 7774 PspOMI G'GGCC_C Cuts at: 3215 3463 3215 Size: 248 7526 PstI C_TGCA'G Cuts at: 830 2190 2475 2688 2703 2954 3285 5126 830 Size: 1360 285 213 15 251 331 1841 3478 Fragments arranged by size: 3478 1841 1360 331 285 251 213 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7774 PvuII CAG'CTG Cuts at: 2143 3687 4924 2143 Size: 1544 1237 4993 Fragments arranged by size: 4993 1544 1237 SacI G_AGCT'C Cuts at: 2046 2250 2292 2463 3419 3798 3996 4748 2046 Size: 204 42 171 956 379 198 752 5072 Fragments arranged by size: 5072 956 752 379 204 198 171 42 SalI G'TCGA_C Cuts at: 1936 5116 1936 Size: 3180 4594 SanDI GG'GwC_CC Cuts at: 3620 3620 Size: 7774 SapI GCTCTTCn'nnn_ Cuts at: 2034 2255 3761 4582 2034 Size: 221 1506 821 5226 Fragments arranged by size: 5226 1506 821 221 SbfI CC_TGCA'GG Cuts at: 2688 5126 2688 Size: 2438 5336 ScaI AGT'ACT Cuts at: 6022 7734 6022 Size: 1712 6062 SfcI C'TryA_G Cuts at: 826 1045 2186 2471 2684 2699 2950 3281 5122 Size: 219 1141 285 213 15 251 331 1841 Cuts at: 5122 6257 6938 7129 826 Size: 1135 681 191 1471 Fragments arranged by size: 1841 1471 1141 1135 681 331 285 251 219 213 191 15 SfoI GGC'GCC Cuts at: 2543 4243 2543 Size: 1700 6074 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7774 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7774 SmaI CCC'GGG Cuts at: 1872 2798 5101 1872 Size: 926 2303 4545 Fragments arranged by size: 4545 2303 926 SmlI C'TyrA_G Cuts at: 820 1017 1943 3048 3935 4160 4564 5878 6779 Size: 197 926 1105 887 225 404 1314 901 Cuts at: 6779 7041 820 Size: 262 1553 Fragments arranged by size: 1553 1314 1105 926 901 887 404 262 225 197 SnaBI TAC'GTA Cuts at: 493 5565 493 Size: 5072 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7774 SphI G_CATG'C Cuts at: 1132 5132 1132 Size: 4000 3774 SspI AAT'ATT Cuts at: 5 52 5698 5 Size: 47 5646 2081 Fragments arranged by size: 5646 2081 47 StuI AGG'CCT Cuts at: 1517 2553 2948 3152 3381 3548 1517 Size: 1036 395 204 229 167 5743 Fragments arranged by size: 5743 1036 395 229 204 167 StyI C'CwwG_G Cuts at: 513 1065 2004 2736 2943 3386 3511 3543 4187 Size: 552 939 732 207 443 125 32 644 Cuts at: 4187 4251 5028 5213 513 Size: 64 777 185 3074 Fragments arranged by size: 3074 939 777 732 644 552 443 207 185 125 64 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2534 2612 3108 3217 3648 3838 3993 Size: 1255 954 78 496 109 431 190 155 Cuts at: 3993 4264 5801 5818 5971 6156 6601 325 Size: 271 1537 17 153 185 445 1498 Fragments arranged by size: 1537 1498 1255 954 496 445 431 271 190 185 155 153 109 78 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2226 2642 4716 Size: 274 80 33 51 1443 249 416 2074 Cuts at: 4716 6020 7732 96 Size: 1304 1712 138 Fragments arranged by size: 2074 1712 1443 1304 416 274 249 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2174 2206 2697 2729 3338 3370 7734 7766 2174 Size: 32 491 32 609 32 4364 32 2182 Fragments arranged by size: 4364 2182 609 491 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4373 1316 Size: 3057 4717 XbaI T'CTAG_A Cuts at: 5110 5110 Size: 7774 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7774 XmaI C'CCGG_G Cuts at: 1870 2796 5099 1870 Size: 926 2303 4545 Fragments arranged by size: 4545 2303 926 XmnI GAAnn'nnTTC Cuts at: 5903 5903 Size: 7774 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5582 276 Size: 53 83 186 936 4048 2468 Fragments arranged by size: 4048 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI XcmI