(Linear) (Six-base) MAPSORT of: KIEE2683.seq Check: 6782 from: 1 to: 3069 KIEE2683.orf With 182 enzymes: SgfI * March 22, 2017 11:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 175 2009 3069 Size: 175 1834 1060 Fragments arranged by size: 1834 1060 175 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 332 2598 2773 3069 Size: 332 2266 175 296 Fragments arranged by size: 2266 332 296 175 AgeI A'CCGG_T Cuts at: 0 2843 3069 Size: 2843 226 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 122 690 3069 Size: 122 568 2379 Fragments arranged by size: 2379 568 122 AlwNI CAG_nnn'CTG Cuts at: 0 208 442 1948 2225 3063 3069 Size: 208 234 1506 277 838 6 Fragments arranged by size: 1506 838 277 234 208 6 ApaI G_GGCC'C Cuts at: 0 1214 1462 3069 Size: 1214 248 1607 Fragments arranged by size: 1607 1214 248 AvaI C'yCGr_G Cuts at: 0 762 791 2744 3069 Size: 762 29 1953 325 Fragments arranged by size: 1953 762 325 29 BamHI G'GATC_C Cuts at: 0 1851 3069 Size: 1851 1218 BanI G'GyrC_C Cuts at: 0 188 536 826 989 1715 2176 2236 3069 Size: 188 348 290 163 726 461 60 833 Fragments arranged by size: 833 726 461 348 290 188 163 60 BanII G_rGCy'C Cuts at: 0 41 245 287 458 769 1214 1414 1462 Size: 41 204 42 171 311 445 200 48 Cuts at: 1462 1505 1642 1793 1991 2356 2518 2743 2909 Size: 43 137 151 198 365 162 225 166 Cuts at: 2909 2936 3069 Size: 27 133 Fragments arranged by size: 445 365 311 225 204 200 198 171 166 162 151 137 133 48 43 42 41 27 BbeI G_GCGC'C Cuts at: 0 540 2240 3069 Size: 540 1700 829 Fragments arranged by size: 1700 829 540 BbsI GAAGACnn'nnnn_ Cuts at: 0 851 1301 2222 2339 2384 3069 Size: 851 450 921 117 45 685 Fragments arranged by size: 921 851 685 450 117 45 BbvCI CC'TCA_GC Cuts at: 0 375 2125 2189 3069 Size: 375 1750 64 880 Fragments arranged by size: 1750 880 375 64 BglI GCCn_nnn'nGGC Cuts at: 0 277 545 1009 2032 2185 3069 Size: 277 268 464 1023 153 884 Fragments arranged by size: 1023 884 464 277 268 153 BglII A'GATC_T Cuts at: 0 553 3069 Size: 553 2516 BlpI GC'TnA_GC Cuts at: 0 2920 3069 Size: 2920 149 Bme1580I G_kGCm'C Cuts at: 0 193 831 1214 1423 1462 2181 3069 Size: 193 638 383 209 39 719 888 Fragments arranged by size: 888 719 638 383 209 193 39 BmgBI CAC'GTC Cuts at: 0 321 1050 3069 Size: 321 729 2019 Fragments arranged by size: 2019 729 321 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 60 444 1941 3069 Size: 60 384 1497 1128 Fragments arranged by size: 1497 1128 384 60 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 694 764 1261 1426 1654 2332 2344 2712 Size: 694 70 497 165 228 678 12 368 Cuts at: 2712 3069 Size: 357 Fragments arranged by size: 694 678 497 368 357 228 165 70 12 Bpu10I CC'TnA_GC Cuts at: 0 375 926 1451 2125 2189 3069 Size: 375 551 525 674 64 880 Fragments arranged by size: 880 674 551 525 375 64 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1028 1915 2176 2544 3069 Size: 1028 887 261 368 525 Fragments arranged by size: 1028 887 525 368 261 BsaI GGTCTCn'nnnn_ Cuts at: 0 2063 2261 2308 3069 Size: 2063 198 47 761 Fragments arranged by size: 2063 761 198 47 BsaHI Gr'CG_yC Cuts at: 0 537 2105 2237 3069 Size: 537 1568 132 832 Fragments arranged by size: 1568 832 537 132 BsaWI w'CCGG_w Cuts at: 0 2843 3069 Size: 2843 226 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 169 199 692 722 1333 1363 2326 2356 Size: 169 30 493 30 611 30 963 30 Cuts at: 2356 2725 2755 2893 2923 3069 Size: 369 30 138 30 146 Fragments arranged by size: 963 611 493 369 169 146 138 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 33 109 466 1189 1303 1324 1327 1351 Size: 33 76 357 723 114 21 3 24 Cuts at: 1351 1354 1357 1360 1861 2341 2434 2455 2458 Size: 3 3 3 501 480 93 21 3 Cuts at: 2458 2461 2464 2800 3069 Size: 3 3 336 269 Fragments arranged by size: 723 501 480 357 336 269 114 93 76 33 24 21 21 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 134 339 406 435 770 959 1392 1455 Size: 134 205 67 29 335 189 433 63 Cuts at: 1455 1563 1678 2018 2168 3053 3069 Size: 108 115 340 150 885 16 Fragments arranged by size: 885 433 340 335 205 189 150 134 115 108 67 63 29 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 383 3069 Size: 383 2686 BsiHKAI G_wGCw'C Cuts at: 0 41 245 287 458 1055 1207 1414 1515 Size: 41 204 42 171 597 152 207 101 Cuts at: 1515 1793 1991 2093 2743 2858 3069 Size: 278 198 102 650 115 211 Fragments arranged by size: 650 597 278 211 207 204 198 171 152 115 102 101 42 41 BsmBI CGTCTCn'nnnn_ Cuts at: 0 350 3069 Size: 350 2719 Bsp1286I G_dGCh'C Cuts at: 0 41 193 245 287 458 769 831 1055 Size: 41 152 52 42 171 311 62 224 Cuts at: 1055 1207 1214 1414 1423 1462 1505 1515 1642 Size: 152 7 200 9 39 43 10 127 Cuts at: 1642 1793 1991 2093 2181 2356 2518 2743 2858 Size: 151 198 102 88 175 162 225 115 Cuts at: 2858 2909 2936 3069 Size: 51 27 133 Fragments arranged by size: 311 225 224 200 198 175 171 162 152 152 151 133 127 115 102 88 62 52 51 43 42 41 39 27 10 9 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 175 954 2009 3069 Size: 175 779 1055 1060 Fragments arranged by size: 1060 1055 779 175 BsrBI CCG'CTC Cuts at: 0 1557 2558 3069 Size: 1557 1001 511 Fragments arranged by size: 1557 1001 511 BsrFI r'CCGG_y Cuts at: 0 1262 2534 2843 3069 Size: 1262 1272 309 226 Fragments arranged by size: 1272 1262 309 226 BsrGI T'GTAC_A Cuts at: 0 637 2711 3069 Size: 637 2074 358 Fragments arranged by size: 2074 637 358 BssHII G'CGCG_C Cuts at: 0 20 3069 Size: 20 3049 BstAPI GCAn_nnn'nTGC Cuts at: 0 3063 3069 Size: 3063 6 BstEII G'GTnAC_C Cuts at: 0 2591 3069 Size: 2591 478 BstXI CCAn_nnnn'nTGG Cuts at: 0 297 3069 Size: 297 2772 BstYI r'GATC_y Cuts at: 0 553 970 1622 1851 2276 3069 Size: 553 417 652 229 425 793 Fragments arranged by size: 793 652 553 425 417 229 Bsu36I CC'TnA_GG Cuts at: 0 443 1916 2890 3069 Size: 443 1473 974 179 Fragments arranged by size: 1473 974 443 179 BtgI C'CryG_G Cuts at: 0 1381 1574 3023 3069 Size: 1381 193 1449 46 Fragments arranged by size: 1449 1381 193 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 871 3069 Size: 871 2198 BtsI GCAGTG_nn' Cuts at: 0 190 460 1231 2499 3069 Size: 190 270 771 1268 570 Fragments arranged by size: 1268 771 570 270 190 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 153 188 3069 Size: 153 35 2881 Fragments arranged by size: 2881 153 35 DrdI GACnn_nn'nnGTC Cuts at: 0 2476 3069 Size: 2476 593 EaeI y'GGCC_r Cuts at: 0 161 482 1082 1590 2037 3069 Size: 161 321 600 508 447 1032 Fragments arranged by size: 1032 600 508 447 321 161 EarI CTCTTCn'nnn_ Cuts at: 0 29 250 1301 1313 1756 2420 2459 2577 Size: 29 221 1051 12 443 664 39 118 Cuts at: 2577 2749 2978 3069 Size: 172 229 91 Fragments arranged by size: 1051 664 443 229 221 172 118 91 39 29 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 598 1249 3069 Size: 598 651 1820 Fragments arranged by size: 1820 651 598 EcoICRI GAG'CTC Cuts at: 0 39 243 285 456 1412 1791 1989 2741 Size: 39 204 42 171 956 379 198 752 Cuts at: 2741 3069 Size: 328 Fragments arranged by size: 956 752 379 328 204 198 171 42 39 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 332 694 764 1261 1426 1654 2332 2344 Size: 332 362 70 497 165 228 678 12 Cuts at: 2344 2598 2712 2773 3069 Size: 254 114 61 296 Fragments arranged by size: 678 497 362 332 296 254 228 165 114 70 61 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 2326 2416 2999 3069 Size: 2326 90 583 70 Fragments arranged by size: 2326 583 90 70 EcoO109I rG'GnC_Cy Cuts at: 0 270 1127 1459 1615 1674 1920 2129 2199 Size: 270 857 332 156 59 246 209 70 Cuts at: 2199 2988 3069 Size: 789 81 Fragments arranged by size: 857 789 332 270 246 209 156 81 70 59 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1782 2067 2778 2847 3007 3069 Size: 1782 285 711 69 160 62 Fragments arranged by size: 1782 711 285 160 69 62 HaeII r_GCGC'y Cuts at: 0 540 2240 2763 2885 3069 Size: 540 1700 523 122 184 Fragments arranged by size: 1700 540 523 184 122 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 142 174 349 381 442 474 606 638 Size: 142 32 175 32 61 32 132 32 Cuts at: 638 1607 1639 1978 2010 2326 2358 2725 2757 Size: 969 32 339 32 316 32 367 32 Cuts at: 2757 3069 Size: 312 Fragments arranged by size: 969 367 339 316 312 175 142 132 61 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 565 3069 Size: 565 2504 KasI G'GCGC_C Cuts at: 0 536 2236 3069 Size: 536 1700 833 Fragments arranged by size: 1700 833 536 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2118 3069 Size: 2118 951 MscI TGG'CCA Cuts at: 0 484 1084 1592 2039 3069 Size: 484 600 508 447 1030 Fragments arranged by size: 1030 600 508 484 447 MspA1I CmG'CkG Cuts at: 0 138 1488 1682 2396 2919 3069 Size: 138 1350 194 714 523 150 Fragments arranged by size: 1350 714 523 194 150 138 NaeI GCC'GGC Cuts at: 0 1264 3069 Size: 1264 1805 NarI GG'CG_CC Cuts at: 0 537 2237 3069 Size: 537 1700 832 Fragments arranged by size: 1700 832 537 NcoI C'CATG_G Cuts at: 0 1381 3023 3069 Size: 1381 1642 46 Fragments arranged by size: 1642 1381 46 NgoMIV G'CCGG_C Cuts at: 0 1262 3069 Size: 1262 1807 NspI r_CATG'y Cuts at: 0 852 882 2011 3069 Size: 852 30 1129 1058 Fragments arranged by size: 1129 1058 852 30 PasI CC'CwG_GG Cuts at: 0 1942 3069 Size: 1942 1127 PflMI CCAn_nnn'nTGG Cuts at: 0 1753 3069 Size: 1753 1316 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 113 145 3069 Size: 113 32 2924 Fragments arranged by size: 2924 113 32 PpuMI rG'GwC_Cy Cuts at: 0 1615 1674 2129 3069 Size: 1615 59 455 940 Fragments arranged by size: 1615 940 455 59 PshAI GACnn'nnGTC Cuts at: 0 323 3069 Size: 323 2746 PspOMI G'GGCC_C Cuts at: 0 1210 1458 3069 Size: 1210 248 1611 Fragments arranged by size: 1611 1210 248 PstI C_TGCA'G Cuts at: 0 185 470 683 698 949 1280 3069 Size: 185 285 213 15 251 331 1789 Fragments arranged by size: 1789 331 285 251 213 185 15 PvuII CAG'CTG Cuts at: 0 138 1682 2919 3069 Size: 138 1544 1237 150 Fragments arranged by size: 1544 1237 150 138 SacI G_AGCT'C Cuts at: 0 41 245 287 458 1414 1793 1991 2743 Size: 41 204 42 171 956 379 198 752 Cuts at: 2743 3069 Size: 326 Fragments arranged by size: 956 752 379 326 204 198 171 42 41 SanDI GG'GwC_CC Cuts at: 0 1615 3069 Size: 1615 1454 SapI GCTCTTCn'nnn_ Cuts at: 0 29 250 1756 2577 3069 Size: 29 221 1506 821 492 Fragments arranged by size: 1506 821 492 221 29 SbfI CC_TGCA'GG Cuts at: 0 683 3069 Size: 683 2386 SfcI C'TryA_G Cuts at: 0 181 466 679 694 945 1276 3069 Size: 181 285 213 15 251 331 1793 Fragments arranged by size: 1793 331 285 251 213 181 15 SfoI GGC'GCC Cuts at: 0 538 2238 3069 Size: 538 1700 831 Fragments arranged by size: 1700 831 538 SmaI CCC'GGG Cuts at: 0 793 3069 Size: 793 2276 SmlI C'TyrA_G Cuts at: 0 1043 1930 2155 2559 3069 Size: 1043 887 225 404 510 Fragments arranged by size: 1043 887 510 404 225 StuI AGG'CCT Cuts at: 0 548 943 1147 1376 1543 3069 Size: 548 395 204 229 167 1526 Fragments arranged by size: 1526 548 395 229 204 167 StyI C'CwwG_G Cuts at: 0 731 938 1381 1506 1538 2182 2246 3023 Size: 731 207 443 125 32 644 64 777 Cuts at: 3023 3069 Size: 46 Fragments arranged by size: 777 731 644 443 207 125 64 46 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 529 607 1103 1212 1643 1833 1988 2259 Size: 529 78 496 109 431 190 155 271 Cuts at: 2259 3069 Size: 810 Fragments arranged by size: 810 529 496 431 271 190 155 109 78 TatI w'GTAC_w Cuts at: 0 221 637 2711 3069 Size: 221 416 2074 358 Fragments arranged by size: 2074 416 358 221 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 169 201 692 724 1333 1365 3069 Size: 169 32 491 32 609 32 1704 Fragments arranged by size: 1704 609 491 169 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2368 3069 Size: 2368 701 XmaI C'CCGG_G Cuts at: 0 791 3069 Size: 791 2278 Enzymes that do cut: AarI AcuI AgeI AlfI AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KasI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SanDI SapI SbfI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BmrI BmtI BsaAI BsaBI BsiEI BsiWI BsmI BspEI BspHI BsrDI BssSI BstBI BstZ17I ClaI DraI DraIII EagI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MslI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacII SalI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XcmI XhoI XmnI ZraI