################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI Asp718I AspEI AspHI AspI AvaI AvaII AvrII BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BpmI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I CpoI DraI DraII DraIII DsaI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HgaI HindIII HpaI KasI KpnI Ksp632I KspI MaeI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SpeI Sse8387I SspBI StyI SwaI TfiI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 298 GACGTC GACGT^C AcyI 298 GACGTC G[GA]^CG[CT]C AflIII 1065 ACATGT A^C[GA][CT]GT Aor51HI 995 AGCGCT AGC^GCT Asp700I 1004 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 407 TGCGCA TGC^GCA BcnI 914 CCGGG CC^[GC]GG BlpI 1147 GCTCAGC GC^T[ACGT]AGC BmyI 815 GGGCAC G[AGT]GC[ACT]^C Bpu1102I 1147 GCTCAGC GC^T[ACGT]AGC BsaAI 543 TACGTA [CT]AC^GT[GA] BsaBI 93 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 298 GACGTC G[GA]^CG[CT]C BsaI 760 GAGACC GGTCTC(1/5) BsmAI 760 GAGAC GTCTC(1/5) Bsp1286I 815 GGGCAC G[AGT]GC[ACT]^C BspDI 318 ATCGAT AT^CGAT BspLU11I 1065 ACATGT A^CATGT BstNI 868 CCTGG CC^[AT]GG CelII 1147 GCTCAGC GC^T[ACGT]AGC Cfr13I 1020 GGGCC G^G[ACGT]CC ClaI 318 ATCGAT AT^CGAT DrdI 856 GACTACACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 995 AGCGCT AGC^GCT EcoRII 868 CCTGG ^CC[AT]GG FauI 1119 CCCGC CCCGC(4/6) FspI 407 TGCGCA TGC^GCA HaeII 995 AGCGCT [GA]GCGC^[CT] HapII 914 CCGG C^CGG Hin1I 298 GACGTC G[GA]^CG[CT]C HincII 562 GTCAAC GT[CT]^[GA]AC HindII 562 GTCAAC GT[CT]^[GA]AC HinfI 1107 GAGTC G^A[ACGT]TC HpaII 914 CCGG C^CGG MamI 93 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 786 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 914 CCGG C^CGG MvaI 868 CCTGG CC^[AT]GG NciI 914 CCGGG CC^[GC]GG PflMI 372 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1107 GAGTC GAGTC(4/5) Sau96I 1020 GGGCC G^G[ACGT]CC SfcI 384 CTATAG C^T[GA][CT]AG SnaBI 543 TACGTA TAC^GTA SphI 663 GCATGC GCATG^C SspI 835 AATATT AAT^ATT StuI 715 AGGCCT AGG^CCT Van91I 372 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1004 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AfaI 546 GTAC GT^AC 1069 GTAC AseI 417 ATTAAT AT^TAAT 1051 ATTAAT AsnI 417 ATTAAT AT^TAAT 1051 ATTAAT BalI 95 TGGCCA TGG^CCA 1095 TGGCCA BamHI 843 GGATCC G^GATCC 1057 GGATCC BanI 229 GGTGCC G^G[CT][GA]CC 816 GGCACC BbsI 1004 GAAGAC GAAGAC(2/6) 553 GTCTTC BpuAI 1004 GAAGAC GAAGAC(2/6) 553 GTCTTC BsaJI 867 CCCTGG C^C[ACGT][ACGT]GG 868 CCTGGG BsrI 1098 CCAGT ACTGG(1/-1) 1154 CCAGT BssKI 868 CCTGG ^CC[ACGT]GG 914 CCGGG BstXI 233 CCATTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 833 CCAATATTGTGG CfoI 408 GCGC GCG^C 996 GCGC EaeI 95 TGGCCA [CT]^GGCC[GA] 1095 TGGCCA HhaI 408 GCGC GCG^C 996 GCGC HinP1I 408 GCGC G^CGC 996 GCGC MboII 1004 GAAGA GAAGA(8/7) 554 TCTTC MluNI 95 TGGCCA TGG^CCA 1095 TGGCCA MscI 95 TGGCCA TGG^CCA 1095 TGGCCA MseI 418 TTAA T^TAA 1052 TTAA MwoI 213 GCTGGTGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 740 GCACTCATCGC NspI 663 GCATGC [GA]CATG^[CT] 1065 ACATGT PshBI 417 ATTAAT AT^TAAT 1051 ATTAAT RsaI 546 GTAC GT^AC 1069 GTAC ScrFI 868 CCTGG CC^[ACGT]GG 914 CCGGG TaqI 79 TCGA T^CGA 319 TCGA Tru9I 418 TTAA T^TAA 1052 TTAA TthHB8I 79 TCGA T^CGA 319 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AluI AlwI BbvI BseRI BsiYI BslI BsmFI BspMI BstF5I BstYI Cac8I DdeI DpnII Fnu4HI FokI HaeIII HphI ItaI MaeII MaeIII MboI MflI MnlI NdeII NlaIII NlaIV Sau3AI SfaNI TseI Tsp45I Tsp509I TspRI XhoII