################################## # NO CUT ENZYMES ( 216 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MflI MluI MnlI MroI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AlwNI 59 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG BalI 122 TGGCCA TGG^CCA BciVI 111 GTATCC GTATCC(6/5) BfaI 68 CTAG C^TAG BsaI 16 GGTCTC GGTCTC(1/5) BsiYI 151 CCAGTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 151 CCAGTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1407I 31 TGTACA T^GTACA BsrGI 31 TGTACA T^GTACA BstF5I 126 CATCC GGATG(2/0) DdeI 57 CTCAG C^T[ACGT]AG DpnII 6 GATC ^GATC EaeI 122 TGGCCA [CT]^GGCC[GA] EarI 224 GAAGAG CTCTTC(1/4) FokI 126 CATCC GGATG(9/13) HaeIII 123 GGCC GG^CC HphI 173 TCACC GGTGA(8/7) Ksp632I 224 GAAGAG CTCTTC(1/4) MaeI 68 CTAG C^TAG MaeIII 191 GTCAC ^GT[ACGT]AC MboI 6 GATC ^GATC MluNI 122 TGGCCA TGG^CCA MscI 122 TGGCCA TGG^CCA NdeII 6 GATC ^GATC PflMI 151 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 93 CTGCAG CTGCA^G Sau3AI 6 GATC ^GATC SspBI 31 TGTACA T^GTACA Tsp45I 191 GTCAC ^GT[GC]AC Van91I 151 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 4 enzymes) # ################################### AfaI 32 GTAC GT^AC 200 GTAC BsmAI 17 GTCTC GTCTC(1/5) 75 GTCTC RsaI 32 GTAC GT^AC 200 GTAC SfcI 48 CTGTAG C^T[GA][CT]AG 93 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### AluI BbvI BsrI Fnu4HI ItaI MboII TseI TspRI