################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BsaBI BsaI BsaWI BseAI BseRI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I ClaI CpoI DraI DraIII EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AviII 386 TGCGCA TGC^GCA BbeI 281 GGCGCC GGCGC^C BbrPI 591 CACGTG CAC^GTG BbsI 129 GAAGAC GAAGAC(2/6) BfaI 167 CTAG C^TAG BlpI 563 GCTCAGC GC^T[ACGT]AGC BpmI 91 CTCCAG CTGGAG(16/14) Bpu1102I 563 GCTCAGC GC^T[ACGT]AGC BpuAI 129 GAAGAC GAAGAC(2/6) BsaAI 591 CACGTG [CT]AC^GT[GA] BsiEI 795 CGACCG CG[GA][CT]^CG BspMI 752 ACCTGC ACCTGC(4/8) BsrBI 681 CCGCTC CCGCTC(-3/-3) BstAPI 462 GCACTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 134 CCAGCCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 266 GGATCT [GA]^GATC[CT] CelII 563 GCTCAGC GC^T[ACGT]AGC DrdI 244 GACCAGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 371 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 386 TGCGCA TGC^GCA HincII 580 GTCAAC GT[CT]^[GA]AC HindII 580 GTCAAC GT[CT]^[GA]AC KasI 281 GGCGCC G^GCGCC MaeI 167 CTAG C^TAG MflI 266 GGATCT [GA]^GATC[CT] MslI 591 CACGTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 86 GCCGGC GCC^GGC NarI 281 GGCGCC GG^CGCC NgoMIV 86 GCCGGC G^CCGGC NspI 596 GCATGT [GA]CATG^[CT] PmaCI 591 CACGTG CAC^GTG PmlI 591 CACGTG CAC^GTG PshAI 573 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SexAI 122 ACCAGGT A^CC[AT]GGT SfoI 281 GGCGCC GGC^GCC SmaI 200 CCCGGG CCC^GGG SmlI 427 CTCAAG C^T[CT][GA]AG XcmI 38 CCAACTCAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 266 GGATCT [GA]^GATC[CT] XmaCI 200 CCCGGG C^CCGGG XmaI 200 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcyI 228 GACGCC G[GA]^CG[CT]C 281 GGCGCC AlwI 12 GATCC GGATC(4/5) 266 GGATC AlwNI 415 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG 571 CAGACTCTG ApaI 209 GGGCCC GGGCC^C 432 GGGCCC AspHI 561 GTGCTC G[AT]GC[AT]^C 716 GTGCTC AvaI 200 CCCGGG C^[CT]CG[GA]G 448 CCCGAG BanI 281 GGCGCC G^G[CT][GA]CC 345 GGCACC BsaHI 228 GACGCC G[GA]^CG[CT]C 281 GGCGCC BsgI 627 CTGCAC GTGCAG(16/14) 607 GTGCAG BsiHKAI 561 GTGCTC G[AT]GC[AT]^C 716 GTGCTC BsmFI 78 GTCCC GGGAC(10/14) 442 GTCCC BsoBI 200 CCCGGG C^[CT]CG[GA]G 448 CCCGAG DpnII 12 GATC ^GATC 267 GATC DsaI 356 CCGTGG C^C[GA][CT]GG 725 CCGTGG EcoT14I 376 CCAAGG C^C[AT][AT]GG 398 CCAAGG HaeII 22 AGCGCC [GA]GCGC^[CT] 281 GGCGCC HgaI 228 GACGC GACGC(5/10) 839 GCGTC Hin1I 228 GACGCC G[GA]^CG[CT]C 281 GGCGCC HinfI 33 GACTC G^A[ACGT]TC 573 GACTC MaeII 523 ACGT A^CGT 592 ACGT MaeIII 242 GTGAC ^GT[ACGT]AC 253 GTCAC MboI 12 GATC ^GATC 267 GATC MboII 129 GAAGA GAAGA(8/7) 617 TCTTC NdeII 12 GATC ^GATC 267 GATC PflMI 414 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 570 CCAGACTCTGG PleI 33 GACTC GAGTC(4/5) 573 GACTC Sau3AI 12 GATC ^GATC 267 GATC StuI 368 AGGCCT AGG^CCT 479 AGGCCT StyI 376 CCAAGG C^C[AT][AT]GG 398 CCAAGG Tsp45I 242 GTGAC ^GT[GC]AC 253 GTCAC TspRI 409 CACTG CA[GC]TG[ACGT][ACGT]^ 625 CACTG Van91I 414 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 570 CCAGACTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AluI AvaII BanII BbvI BcnI BmyI BsaJI BsiYI BslI Bsp1286I BsrFI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI SfaNI TseI