Restriction map for
pFN21AE5363
(Linear) (Six-base) MAPSORT of: KIEE5363.seq Check: 5922 from: 1 to: 897
KIEE5363.orf
With 182 enzymes: SgfI *
January 29, 2014 11:57 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 897
Size: 258 639
AlwNI CAG_nnn'CTG
Cuts at: 0 420 576 897
Size: 420 156 321
Fragments arranged by size:
420 321 156
ApaI G_GGCC'C
Cuts at: 0 213 436 897
Size: 213 223 461
Fragments arranged by size:
461 223 213
AvaI C'yCGr_G
Cuts at: 0 200 448 897
Size: 200 248 449
Fragments arranged by size:
449 248 200
BanI G'GyrC_C
Cuts at: 0 281 345 897
Size: 281 64 552
Fragments arranged by size:
552 281 64
BanII G_rGCy'C
Cuts at: 0 8 213 436 652 897
Size: 8 205 223 216 245
Fragments arranged by size:
245 223 216 205 8
BbeI G_GCGC'C
Cuts at: 0 285 897
Size: 285 612
BbsI GAAGACnn'nnnn_
Cuts at: 0 136 897
Size: 136 761
BlpI GC'TnA_GC
Cuts at: 0 564 897
Size: 564 333
Bme1580I G_kGCm'C
Cuts at: 0 213 436 897
Size: 213 223 461
Fragments arranged by size:
461 223 213
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 897
Size: 76 821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 897
Size: 412 485
BsaAI yAC'GTr
Cuts at: 0 593 897
Size: 593 304
BsaHI Gr'CG_yC
Cuts at: 0 229 282 897
Size: 229 53 615
Fragments arranged by size:
615 229 53
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 544 574 655 685 897
Size: 544 30 81 30 212
Fragments arranged by size:
544 212 81 30 30
BseYI C'CCAG_C
Cuts at: 0 286 536 782 897
Size: 286 250 246 115
Fragments arranged by size:
286 250 246 115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 612 628 897
Size: 612 16 269
Fragments arranged by size:
612 269 16
BsiEI CG_ry'CG
Cuts at: 0 798 897
Size: 798 99
BsiHKAI G_wGCw'C
Cuts at: 0 565 720 897
Size: 565 155 177
Fragments arranged by size:
565 177 155
Bsp1286I G_dGCh'C
Cuts at: 0 8 213 436 565 652 720 897
Size: 8 205 223 129 87 68 177
Fragments arranged by size:
223 205 177 129 87 68 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 761 897
Size: 761 136
BsrBI CCG'CTC
Cuts at: 0 683 897
Size: 683 214
BsrFI r'CCGG_y
Cuts at: 0 86 184 763 897
Size: 86 98 579 134
Fragments arranged by size:
579 134 98 86
BstAPI GCAn_nnn'nTGC
Cuts at: 0 468 897
Size: 468 429
BstXI CCAn_nnnn'nTGG
Cuts at: 0 141 897
Size: 141 756
BstYI r'GATC_y
Cuts at: 0 266 897
Size: 266 631
BtgI C'CryG_G
Cuts at: 0 356 725 897
Size: 356 369 172
Fragments arranged by size:
369 356 172
BtsI GCAGTG_nn'
Cuts at: 0 408 624 897
Size: 408 216 273
Fragments arranged by size:
408 273 216
DrdI GACnn_nn'nnGTC
Cuts at: 0 250 897
Size: 250 647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 258 897
Size: 76 182 639
Fragments arranged by size:
639 182 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 375 897
Size: 375 522
EcoO109I rG'GnC_Cy
Cuts at: 0 209 210 432 897
Size: 209 1 222 465
Fragments arranged by size:
465 222 209 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 483 897
Size: 483 414
FspI TGC'GCA
Cuts at: 0 388 897
Size: 388 509
FspAI rTGC'GCAy
Cuts at: 0 388 897
Size: 388 509
HaeII r_GCGC'y
Cuts at: 0 26 285 897
Size: 26 259 612
Fragments arranged by size:
612 259 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 355 387 496 528 634 666 897
Size: 355 32 109 32 106 32 231
Fragments arranged by size:
355 231 109 106 32 32 32
HincII GTy'rAC
Cuts at: 0 582 897
Size: 582 315
KasI G'GCGC_C
Cuts at: 0 281 897
Size: 281 616
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 897
Size: 710 187
MslI CAynn'nnrTG
Cuts at: 0 595 897
Size: 595 302
MspA1I CmG'CkG
Cuts at: 0 239 278 420 701 821 860 897
Size: 239 39 142 281 120 39 37
Fragments arranged by size:
281 239 142 120 39 39 37
NaeI GCC'GGC
Cuts at: 0 88 897
Size: 88 809
NarI GG'CG_CC
Cuts at: 0 282 897
Size: 282 615
NgoMIV G'CCGG_C
Cuts at: 0 86 897
Size: 86 811
NspI r_CATG'y
Cuts at: 0 600 897
Size: 600 297
PflMI CCAn_nnn'nTGG
Cuts at: 0 420 576 897
Size: 420 156 321
Fragments arranged by size:
420 321 156
PmlI CAC'GTG
Cuts at: 0 593 897
Size: 593 304
PshAI GACnn'nnGTC
Cuts at: 0 577 897
Size: 577 320
PspOMI G'GGCC_C
Cuts at: 0 209 432 897
Size: 209 223 465
Fragments arranged by size:
465 223 209
SexAI A'CCwGG_T
Cuts at: 0 122 897
Size: 122 775
SfoI GGC'GCC
Cuts at: 0 283 897
Size: 283 614
SmaI CCC'GGG
Cuts at: 0 202 897
Size: 202 695
SmlI C'TyrA_G
Cuts at: 0 427 897
Size: 427 470
StuI AGG'CCT
Cuts at: 0 370 481 897
Size: 370 111 416
Fragments arranged by size:
416 370 111
StyI C'CwwG_G
Cuts at: 0 376 398 897
Size: 376 22 499
Fragments arranged by size:
499 376 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 113 271 703 897
Size: 113 158 432 194
Fragments arranged by size:
432 194 158 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 92 124 544 576 655 687 897
Size: 92 32 420 32 79 32 210
Fragments arranged by size:
420 210 92 79 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 45 897
Size: 45 852
XmaI C'CCGG_G
Cuts at: 0 200 897
Size: 200 697
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BanI BanII BbeI BbsI
BlpI Bme1580I BpmI BpuEI BsaAI BsaHI BsaXI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstAPI
BstXI BstYI BtgI BtsI DrdI Eco57MI EcoNI EcoO109I
FalI FspI FspAI HaeII Hin4I HincII KasI MmeI
MslI MspA1I NaeI NarI NgoMIV NspI PflMI PmlI
PshAI PspOMI SexAI SfoI SmaI SmlI StuI StyI
TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI
Bpu10I BsaI BsaBI BsaWI BseRI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EaeI
EagI EarI EciI EcoICRI EcoRI EcoRV FseI HindIII
HpaI KpnI MfeI MluI MscI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PpiI PpuMI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TatI Tth111I XbaI XhoI XmnI ZraI