Restriction map for
pFN21AE2409
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AvaI AviII AvrII BalI BanII
BbeI BbrPI BbsI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HindIII KasI
KspI MaeI MaeII MaeIII MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NarI NciI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 53 GGTACC G^GTACC
Asp718I 53 GGTACC G^GTACC
BamHI 479 GGATCC G^GATCC
BanI 53 GGTACC G^G[CT][GA]CC
BbvCI 327 GCTGAGG CCTCAGC(-5/-2)
BpmI 83 CTGGAG CTGGAG(16/14)
BsaI 460 GGTCTC GGTCTC(1/5)
BseRI 113 CTCCTC GAGGAG(10/8)
BsiEI 29 CGGTCG CG[GA][CT]^CG
BsiYI 37 CCGGCCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 37 CCGGCCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 461 GTCTC GTCTC(1/5)
BsmI 373 GCATTC GAATGC(1/-1)
BspMI 103 GCAGGT ACCTGC(4/8)
BstAPI 103 GCAGGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 479 GGATCC [GA]^GATC[CT]
DpnII 480 GATC ^GATC
EarI 76 GAAGAG CTCTTC(1/4)
EcoT14I 245 CCAAGG C^C[AT][AT]GG
HaeII 18 GGCGCT [GA]GCGC^[CT]
HincII 357 GTTAAC GT[CT]^[GA]AC
HindII 357 GTTAAC GT[CT]^[GA]AC
HpaI 357 GTTAAC GTT^AAC
HphI 106 GGTGA GGTGA(8/7)
KpnI 53 GGTACC GGTAC^C
Ksp632I 76 GAAGAG CTCTTC(1/4)
MboI 480 GATC ^GATC
MflI 479 GGATCC [GA]^GATC[CT]
NaeI 36 GCCGGC GCC^GGC
NdeII 480 GATC ^GATC
NgoMIV 36 GCCGGC G^CCGGC
Sau3AI 480 GATC ^GATC
ScaI 530 AGTACT AGT^ACT
StyI 245 CCAAGG C^C[AT][AT]GG
TaqI 32 TCGA T^CGA
TspRI 215 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 32 TCGA T^CGA
XhoII 479 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 54 GTAC GT^AC
531 GTAC
AlwI 480 GATCC GGATC(4/5)
479 GGATC
AvaII 242 GGACC G^G[AT]CC
641 GGACC
BsrFI 36 GCCGGC [GA]^CCGG[CT]
50 GCCGGT
BstF5I 168 GGATG GGATG(2/0)
303 GGATG
CfoI 19 GCGC GCG^C
146 GCGC
Cfr10I 36 GCCGGC [GA]^CCGG[CT]
50 GCCGGT
DdeI 328 CTGAG C^T[ACGT]AG
577 CTCAG
FokI 168 GGATG GGATG(9/13)
303 GGATG
HaeIII 39 GGCC GG^CC
444 GGCC
HapII 37 CCGG C^CGG
51 CCGG
HhaI 19 GCGC GCG^C
146 GCGC
HinP1I 19 GCGC G^CGC
146 GCGC
HinfI 79 GAGTC G^A[ACGT]TC
172 GAGTC
HpaII 37 CCGG C^CGG
51 CCGG
MspA1I 325 CAGCTG C[AC]G^C[GT]G
425 CAGCTG
MspI 37 CCGG C^CGG
51 CCGG
NlaIII 354 CATG CATG^
369 CATG
PleI 79 GAGTC GAGTC(4/5)
172 GAGTC
PvuII 325 CAGCTG CAG^CTG
425 CAGCTG
RsaI 54 GTAC GT^AC
531 GTAC
Tsp509I 471 AATT ^AATT
592 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsrI
BssKI BstNI Cac8I Cfr13I EcoRII
Fnu4HI ItaI MboII MnlI MseI
MvaI MwoI NlaIV PstI Sau96I
ScrFI SfaNI SfcI Tru9I TseI