Restriction map for
pFN21AE2409
(Circular) (Six-base) MAPSORT of: pFN21AE2409.seq Check: 6037 from: 1 to: 5368
pFN21AE2409 5368 bp
With 182 enzymes: SgfI *
April 24, 2012 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2099 2099
Size: 5368
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3178 278
Size: 53 83 186 936 1642 2468
Fragments arranged by size:
2468 1642 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2711 1937
Size: 774 4594
Acc65I G'GTAC_C
Cuts at: 2058 2689 2058
Size: 631 4737
AclI AA'CG_TT
Cuts at: 1563 3495 3868 1563
Size: 1932 373 3063
Fragments arranged by size:
3063 1932 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2277 2412 3431 4815 5083 1632
Size: 645 135 1019 1384 268 1917
Fragments arranged by size:
1917 1384 1019 645 268 135
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5171
AflIII A'CryG_T
Cuts at: 1203 4267 5244 1203
Size: 3064 977 1327
Fragments arranged by size:
3064 1327 977
AgeI A'CCGG_T
Cuts at: 4199 4199
Size: 5368
AhdI GACnn_n'nnGTC
Cuts at: 4097 4097
Size: 5368
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5368
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5368
AlwNI CAG_nnn'CTG
Cuts at: 3111 4683 3111
Size: 1572 3796
ApaLI G'TGCA_C
Cuts at: 3425 4581 3425
Size: 1156 4212
ApoI r'AATT_y
Cuts at: 1493 2677 2903 3055 5164 1493
Size: 1184 226 152 2109 1697
Fragments arranged by size:
2109 1697 1184 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3922 160
Size: 872 2890 1606
Fragments arranged by size:
2890 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5368
AvaI C'yCGr_G
Cuts at: 1870 1943 2693 1870
Size: 73 750 4545
Fragments arranged by size:
4545 750 73
BamHI G'GATC_C
Cuts at: 2484 2698 3065 2484
Size: 214 367 4787
Fragments arranged by size:
4787 367 214
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2058 2689 4144 618
Size: 325 217 627 271 631 1455 1842
Fragments arranged by size:
1842 1455 631 627 325 271 217
BanII G_rGCy'C
Cuts at: 1391 2687 1391
Size: 1296 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4397
BbvCI CC'TCA_GC
Cuts at: 2333 2333
Size: 5368
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3559 3593 1903
Size: 34 1622 34 3678
Fragments arranged by size:
3678 1622 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3260 4470 3260
Size: 1210 4158
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5368
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3979 136
Size: 107 122 71 631 605 2307 1525
Fragments arranged by size:
2307 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5363 5363
Size: 5368
BlpI GC'TnA_GC
Cuts at: 2785 2785
Size: 5368
Bme1580I G_kGCm'C
Cuts at: 1790 3429 4585 1790
Size: 1639 1156 2573
Fragments arranged by size:
2573 1639 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5368
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2220 3099 4057
Size: 416 53 83 399 24 803 879 958
Cuts at: 4057 442
Size: 1753
Fragments arranged by size:
1753 958 879 803 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2109 4028 1239
Size: 870 1919 2579
Fragments arranged by size:
2579 1919 870
Bpu10I CC'TnA_GC
Cuts at: 2333 2333
Size: 5368
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3493 4358 4656 3493
Size: 865 298 4205
Fragments arranged by size:
4205 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2471 4031 882
Size: 617 972 1560 2219
Fragments arranged by size:
2219 1560 972 617
BsaAI yAC'GTr
Cuts at: 493 1204 3159 493
Size: 711 1955 2702
Fragments arranged by size:
2702 1955 711
BsaBI GATnn'nnATC
Cuts at: 5362 5362
Size: 5368
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3175 3557 4192 275
Size: 53 83 186 936 1642 382 635 1451
Fragments arranged by size:
1642 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3801 4199 4473 4620 5031 3801
Size: 398 274 147 411 4138
Fragments arranged by size:
4138 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5143
Fragments arranged by size:
5143 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2109 1186
Size: 923 4445
BseYI C'CCAG_C
Cuts at: 4571 5067 4571
Size: 496 4872
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4795
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2037 2818 3579 4607 1540
Size: 275 186 36 781 761 1028 2301
Fragments arranged by size:
2301 1028 781 761 275 186 36
BsiHKAI G_wGCw'C
Cuts at: 3429 3514 4585 3429
Size: 85 1071 4212
Fragments arranged by size:
4212 1071 85
BsmI GAATG_Cn'
Cuts at: 2378 2879 2972 2378
Size: 501 93 4774
Fragments arranged by size:
4774 501 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2687 3429 3514 4585 1391
Size: 399 897 742 85 1071 2174
Fragments arranged by size:
2174 1071 897 742 399 85
BspEI T'CCGG_A
Cuts at: 5031 5031
Size: 5368
BspHI T'CATG_A
Cuts at: 3257 4987 3257
Size: 1730 3638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2099 2723 844
Size: 1255 624 3489
Fragments arranged by size:
3489 1255 624
BsrBI CCG'CTC
Cuts at: 1394 1823 3255 1394
Size: 429 1432 3507
Fragments arranged by size:
3507 1432 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3863 4037 65
Size: 1183 2615 174 1396
Fragments arranged by size:
2615 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2041 2055 4012 4199 1700
Size: 110 231 14 1957 187 2869
Fragments arranged by size:
2869 1957 231 187 110 14
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5368
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5368
BssSI C'ACGA_G
Cuts at: 3428 4440 3428
Size: 1012 4356
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2114 794
Size: 1320 4048
BstBI TT'CG_AA
Cuts at: 4172 4172
Size: 5368
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5368
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5368
BstYI r'GATC_y
Cuts at: 1972 2484 2698 3065 3450 3467 4919 5005 5017
Size: 512 214 367 385 17 1452 86 12
Cuts at: 5017 5363 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 512 385 367 346 214 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3877
Fragments arranged by size:
3877 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4629
Fragments arranged by size:
4629 683 56
BtsI GCAGTG_nn'
Cuts at: 2219 2884 3678 3698 2219
Size: 665 794 20 3889
Fragments arranged by size:
3889 794 665 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5368
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5333
DraI TTT'AAA
Cuts at: 2672 2672
Size: 5368
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4375 809
Size: 507 3059 1802
Fragments arranged by size:
3059 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2815 3704 5333
Size: 54 1425 50 275 18 985 889 1629
Cuts at: 5333 8
Size: 43
Fragments arranged by size:
1629 1425 985 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2815 1537
Size: 275 1003 4090
Fragments arranged by size:
4090 1003 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 3298 1353
Size: 723 1222 3423
Fragments arranged by size:
3423 1222 723
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3955 4329 4475 387
Size: 1064 138 2366 374 146 1280
Fragments arranged by size:
2366 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2277 2412 3431 4028 4815 5083
Size: 393 477 168 135 1019 597 787 268
Cuts at: 5083 1239
Size: 1524
Fragments arranged by size:
1524 1019 787 597 477 393 268 168
135
EcoRI G'AATT_C
Cuts at: 2677 3055 2677
Size: 378 4990
FspI TGC'GCA
Cuts at: 3874 3874
Size: 5368
HaeII r_GCGC'y
Cuts at: 1678 2027 4515 1678
Size: 349 2488 2531
Fragments arranged by size:
2531 2488 349
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2451 2483 2577 2609 4009
Size: 32 135 32 879 32 94 32 1400
Cuts at: 4009 4041 4083 4115 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1400 879 135 94 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2364 2712 2958 669
Size: 988 281 426 348 246 3079
Fragments arranged by size:
3079 988 426 348 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5368
HpaI GTT'AAC
Cuts at: 2364 2958 2364
Size: 594 4774
KpnI G_GTAC'C
Cuts at: 2062 2693 2062
Size: 631 4737
MfeI C'AATT_G
Cuts at: 2967 2967
Size: 5368
MluI A'CGCG_T
Cuts at: 5244 5244
Size: 5368
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4482 4666 1305
Size: 3177 184 2007
Fragments arranged by size:
3177 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3546
Fragments arranged by size:
3546 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3326 3685 3844 518
Size: 648 2160 359 159 2042
Fragments arranged by size:
2160 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2332 2432 2784 3461 4609 4854 1606
Size: 726 100 352 677 1148 245 2120
Fragments arranged by size:
2120 1148 726 677 352 245 100
NaeI GCC'GGC
Cuts at: 2043 2043
Size: 5368
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3877
Fragments arranged by size:
3877 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5368
NgoMIV G'CCGG_C
Cuts at: 2041 2041
Size: 5368
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5368
NotI GC'GGCC_GC
Cuts at: 2815 2815
Size: 5368
NspI r_CATG'y
Cuts at: 1132 1229 2726 4271 1132
Size: 97 1497 1545 2229
Fragments arranged by size:
2229 1545 1497 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5368
PciI A'CATG_T
Cuts at: 4267 4267
Size: 5368
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5368
PmeI GTTT'AAAC
Cuts at: 2672 2672
Size: 5368
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3437 3469 4950 4982 3437
Size: 32 1481 32 3823
Fragments arranged by size:
3823 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5368
PsiI TTA'TAA
Cuts at: 2938 2938
Size: 5368
PstI C_TGCA'G
Cuts at: 830 2110 2146 2437 2720 830
Size: 1280 36 291 283 3478
Fragments arranged by size:
3478 1280 291 283 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5368
PvuII CAG'CTG
Cuts at: 2332 2432 2332
Size: 100 5268
SalI G'TCGA_C
Cuts at: 1936 2710 1936
Size: 774 4594
SbfI CC_TGCA'GG
Cuts at: 2720 2720
Size: 5368
ScaI AGT'ACT
Cuts at: 2537 3616 5328 2537
Size: 1079 1712 2577
Fragments arranged by size:
2577 1712 1079
SfcI C'TryA_G
Cuts at: 826 1045 2106 2142 2433 2716 3851 4532 4723
Size: 219 1061 36 291 283 1135 681 191
Cuts at: 4723 826
Size: 1471
Fragments arranged by size:
1471 1135 1061 681 291 283 219 191
36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5368
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5368
SmaI CCC'GGG
Cuts at: 1872 2695 1872
Size: 823 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3472 4373 4635 820
Size: 197 926 1529 901 262 1553
Fragments arranged by size:
1553 1529 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3159 493
Size: 2666 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5368
SphI G_CATG'C
Cuts at: 1132 2726 1132
Size: 1594 3774
SspI AAT'ATT
Cuts at: 5 52 3292 5
Size: 47 3240 2081
Fragments arranged by size:
3240 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5368
StyI C'CwwG_G
Cuts at: 513 1065 2004 2250 2807 513
Size: 552 939 246 557 3074
Fragments arranged by size:
3074 939 557 552 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2539 3395 3412 3565 3750 4195 325
Size: 1255 959 856 17 153 185 445 1498
Fragments arranged by size:
1498 1255 959 856 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2535 3614 5326
Size: 274 80 33 51 1443 558 1079 1712
Cuts at: 5326 96
Size: 138
Fragments arranged by size:
1712 1443 1079 558 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5328 5360 5328
Size: 32 5336
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5368
XbaI T'CTAG_A
Cuts at: 2704 2704
Size: 5368
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5368
XmaI C'CCGG_G
Cuts at: 1870 2693 1870
Size: 823 4545
XmnI GAAnn'nnTTC
Cuts at: 3497 3497
Size: 5368
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3176 276
Size: 53 83 186 936 1642 2468
Fragments arranged by size:
2468 1642 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI