################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BclI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BseRI BsiHKAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MslI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuII RcaI RsrII SacI SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 751 GGTACC G^GTACC AccII 333 CGCG CG^CG AflII 759 CTTAAG C^TTAAG Asp718I 751 GGTACC G^GTACC AvaI 519 CTCGAG C^[CT]CG[GA]G BbsI 156 GAAGAC GAAGAC(2/6) BciVI 476 GTATCC GTATCC(6/5) BfaI 619 CTAG C^TAG BfrI 759 CTTAAG C^TTAAG BpmI 319 CTCCAG CTGGAG(16/14) BpuAI 156 GAAGAC GAAGAC(2/6) BsaI 175 GGTCTC GGTCTC(1/5) BsaWI 7 TCCGGT [AT]^CCGG[AT] BsgI 359 CTGCAC GTGCAG(16/14) BsiEI 583 CGATCG CG[GA][CT]^CG BsmAI 176 GTCTC GTCTC(1/5) BsmFI 58 GGGAC GGGAC(10/14) BsoBI 519 CTCGAG C^[CT]CG[GA]G Bsp1407I 128 TGTACA T^GTACA BsrGI 128 TGTACA T^GTACA BstNI 13 CCTGG CC^[AT]GG BstUI 333 CGCG CG^CG EarI 241 CTCTTC CTCTTC(1/4) EcoRI 707 GAATTC G^AATTC EcoRII 13 CCTGG ^CC[AT]GG HgaI 595 GCGTC GACGC(5/10) HphI 140 TCACC GGTGA(8/7) KpnI 751 GGTACC GGTAC^C Ksp632I 241 CTCTTC CTCTTC(1/4) KspI 332 CCGCGG CCGC^GG MaeI 619 CTAG C^TAG MvaI 13 CCTGG CC^[AT]GG MvnI 333 CGCG CG^CG NaeI 395 GCCGGC GCC^GGC NcoI 510 CCATGG C^CATGG NgoMIV 395 GCCGGC G^CCGGC PaeR7I 519 CTCGAG C^TCGAG PleI 515 GACTC GAGTC(4/5) PpuMI 337 GGGTCCT [GA]G^G[AT]CC[CT] PstI 326 CTGCAG CTGCA^G PvuI 583 CGATCG CGAT^CG SacII 332 CCGCGG CCGC^GG ScaI 523 AGTACT AGT^ACT SspBI 128 TGTACA T^GTACA TfiI 819 GATTC G^A[AT]TC XcmI 407 CCATGTATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 519 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 659 GAATTT [GA]^AATT[CT] 707 GAATTC ApoI 659 GAATTT [GA]^AATT[CT] 707 GAATTC BanI 196 GGCACC G^G[CT][GA]CC 751 GGTACC BglI 494 GCCCCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 835 GCCAATGGGGC BmyI 195 GGGCAC G[AGT]GC[ACT]^C 842 GGGCAC Bsp1286I 195 GGGCAC G[AGT]GC[ACT]^C 842 GGGCAC BsrFI 199 ACCGGC [GA]^CCGG[CT] 395 GCCGGC BstAPI 352 GCATTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 826 GCAATCCCTGC BstF5I 18 CATCC GGATG(2/0) 489 GGATG Cfr10I 199 ACCGGC [GA]^CCGG[CT] 395 GCCGGC DsaI 332 CCGCGG C^C[GA][CT]GG 510 CCATGG EcoNI 126 CCTGTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 480 CCTTAAAAAGG EcoT14I 497 CCTTGG C^C[AT][AT]GG 510 CCATGG FauI 43 GCGGG CCCGC(4/6) 334 GCGGG FokI 18 CATCC GGATG(9/13) 489 GGATG HinfI 515 GACTC G^A[ACGT]TC 819 GATTC MaeII 115 ACGT A^CGT 221 ACGT MspA1I 41 CAGCGG C[AC]G^C[GT]G 332 CCGCGG SfaNI 108 GATGC GCATC(5/9) 17 GCATC SfcI 326 CTGCAG C^T[GA][CT]AG 400 CTACAG StyI 497 CCTTGG C^C[AT][AT]GG 510 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BcnI BsaJI BsiYI BslI BsrI BssKI Cac8I Cfr13I DdeI DpnII DraII EcoO109I Fnu4HI HaeIII HapII HpaII ItaI MboI MboII MnlI MseI MspI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SmlI TaqI Tru9I TseI Tsp509I TspRI TthHB8I