Restriction map for
pFN21AE6292
(Circular) (Six-base) MAPSORT of: pFN21AE6292.seq Check: 9709 from: 1 to: 5608
pFN21AE6292 5608 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3418 278
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2951 1937
Size: 1014 4594
Acc65I G'GTAC_C
Cuts at: 2756 2929 2756
Size: 173 5435
AclI AA'CG_TT
Cuts at: 1563 3735 4108 1563
Size: 2172 373 3063
Fragments arranged by size:
3063 2172 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2180 3671 5055 5323 1632
Size: 548 1491 1384 268 1917
Fragments arranged by size:
1917 1491 1384 548 268
AflII C'TTAA_G
Cuts at: 820 1017 2764 820
Size: 197 1747 3664
Fragments arranged by size:
3664 1747 197
AflIII A'CryG_T
Cuts at: 1203 4507 5484 1203
Size: 3304 977 1327
Fragments arranged by size:
3304 1327 977
AgeI A'CCGG_T
Cuts at: 4439 4439
Size: 5608
AhdI GACnn_n'nnGTC
Cuts at: 4337 4337
Size: 5608
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5608
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5608
AlwNI CAG_nnn'CTG
Cuts at: 3351 4923 3351
Size: 1572 4036
ApaLI G'TGCA_C
Cuts at: 3665 4821 3665
Size: 1156 4452
ApoI r'AATT_y
Cuts at: 1493 2664 2712 2917 3143 3295 5404 1493
Size: 1171 48 205 226 152 2109 1697
Fragments arranged by size:
2109 1697 1171 226 205 152 48
AseI AT'TA_AT
Cuts at: 160 1032 4162 160
Size: 872 3130 1606
Fragments arranged by size:
3130 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
AvaI C'yCGr_G
Cuts at: 1870 1943 2524 2933 1870
Size: 73 581 409 4545
Fragments arranged by size:
4545 581 409 73
BamHI G'GATC_C
Cuts at: 2938 3305 2938
Size: 367 5241
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2201 2756 2929 4384 618
Size: 325 217 627 414 555 173 1455 1842
Fragments arranged by size:
1842 1455 627 555 414 325 217 173
BanII G_rGCy'C
Cuts at: 1391 2927 1391
Size: 1536 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2168 928
Size: 971 269 4368
Fragments arranged by size:
4368 971 269
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3799 3833 1903
Size: 34 1862 34 3678
Fragments arranged by size:
3678 1862 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2492 3500 4710 2492
Size: 1008 1210 3390
Fragments arranged by size:
3390 1210 1008
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2505 2846 4219
Size: 107 122 71 631 605 833 341 1373
Cuts at: 4219 136
Size: 1525
Fragments arranged by size:
1525 1373 833 631 605 341 122 107
71
BglII A'GATC_T
Cuts at: 5603 5603
Size: 5608
BlpI GC'TnA_GC
Cuts at: 3025 3025
Size: 5608
Bme1580I G_kGCm'C
Cuts at: 1790 2204 2851 3669 4825 1790
Size: 414 647 818 1156 2573
Fragments arranged by size:
2573 1156 818 647 414
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5608
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2091 2684 3339
Size: 416 53 83 399 24 674 593 655
Cuts at: 3339 4297 442
Size: 958 1753
Fragments arranged by size:
1753 958 674 655 593 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2309 4268 1239
Size: 1070 1959 2579
Fragments arranged by size:
2579 1959 1070
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2157 3733 4598 4896 2157
Size: 1576 865 298 2869
Fragments arranged by size:
2869 1576 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2186 4271 882
Size: 617 687 2085 2219
Fragments arranged by size:
2219 2085 687 617
BsaAI yAC'GTr
Cuts at: 493 1204 3399 493
Size: 711 2195 2702
Fragments arranged by size:
2702 2195 711
BsaBI GATnn'nnATC
Cuts at: 5602 5602
Size: 5608
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3415 3797 4432 275
Size: 53 83 186 936 1882 382 635 1451
Fragments arranged by size:
1882 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2012 4041 4439 4713 4860 5271 2012
Size: 2029 398 274 147 411 2349
Fragments arranged by size:
2349 2029 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5383
Fragments arranged by size:
5383 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5608
BseYI C'CCAG_C
Cuts at: 4811 5307 4811
Size: 496 5112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2349 1163
Size: 573 613 4422
Fragments arranged by size:
4422 613 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2591 3058 3819 4847 1540
Size: 275 186 590 467 761 1028 2301
Fragments arranged by size:
2301 1028 761 590 467 275 186
BsiHKAI G_wGCw'C
Cuts at: 3669 3754 4825 3669
Size: 85 1071 4452
Fragments arranged by size:
4452 1071 85
BsmI GAATG_Cn'
Cuts at: 3119 3212 3119
Size: 93 5515
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2204 2851 2927 3669 3754 4825 1391
Size: 399 414 647 76 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 647 414 399 85 76
BspEI T'CCGG_A
Cuts at: 5271 5271
Size: 5608
BspHI T'CATG_A
Cuts at: 3497 5227 3497
Size: 1730 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2963 844
Size: 2119 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3495 1394
Size: 429 1672 3507
Fragments arranged by size:
3507 1672 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4103 4277 65
Size: 1183 2855 174 1396
Fragments arranged by size:
2855 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2204 2400 4252 4439 1700
Size: 110 394 196 1852 187 2869
Fragments arranged by size:
2869 1852 394 196 187 110
BsrGI T'GTAC_A
Cuts at: 96 2133 96
Size: 2037 3571
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5608
BssSI C'ACGA_G
Cuts at: 3668 4680 3668
Size: 1012 4596
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2363 2837 794
Size: 1569 474 3565
Fragments arranged by size:
3565 1569 474
BstBI TT'CG_AA
Cuts at: 4412 4412
Size: 5608
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5608
BstYI r'GATC_y
Cuts at: 1972 2938 3305 3690 3707 5159 5245 5257 5603
Size: 966 367 385 17 1452 86 12 346
Cuts at: 5603 1972
Size: 1977
Fragments arranged by size:
1977 1452 966 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2337 2515 513
Size: 552 1272 178 3606
Fragments arranged by size:
3606 1272 552 178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4869
Fragments arranged by size:
4869 683 56
BtsI GCAGTG_nn'
Cuts at: 3124 3918 3938 3124
Size: 794 20 4794
Fragments arranged by size:
4794 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2371 2406 529
Size: 35 1807 35 3731
Fragments arranged by size:
3731 1807 35 35
DraI TTT'AAA
Cuts at: 2912 2912
Size: 5608
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4615 809
Size: 507 3299 1802
Fragments arranged by size:
3299 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3055 3944 5573
Size: 54 1425 50 275 18 1225 889 1629
Cuts at: 5573 8
Size: 43
Fragments arranged by size:
1629 1425 1225 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3055 1537
Size: 275 1243 4090
Fragments arranged by size:
4090 1243 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2252 3538 1353
Size: 899 1286 3423
Fragments arranged by size:
3423 1286 899
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4195 4569 4715 387
Size: 1064 138 2606 374 146 1280
Fragments arranged by size:
2606 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2309 3671 4268 5055 5323 1239
Size: 393 548 129 1362 597 787 268 1524
Fragments arranged by size:
1524 1362 787 597 548 393 268 129
EcoNI CCTnn'n_nnAGG
Cuts at: 2135 2489 2135
Size: 354 5254
EcoO109I rG'GnC_Cy
Cuts at: 2140 2343 2627 2140
Size: 203 284 5121
Fragments arranged by size:
5121 284 203
EcoRI G'AATT_C
Cuts at: 2712 2917 3295 2712
Size: 205 378 5025
Fragments arranged by size:
5025 378 205
FspI TGC'GCA
Cuts at: 4114 4114
Size: 5608
HaeII r_GCGC'y
Cuts at: 1678 4755 1678
Size: 3077 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2155 2187 4249 4281 4323
Size: 32 135 32 583 32 2062 32 42
Cuts at: 4323 4355 1373
Size: 32 2626
Fragments arranged by size:
2626 2062 583 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2952 3198 669
Size: 988 281 1014 246 3079
Fragments arranged by size:
3079 1014 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5608
HpaI GTT'AAC
Cuts at: 3198 3198
Size: 5608
KpnI G_GTAC'C
Cuts at: 2760 2933 2760
Size: 173 5435
MfeI C'AATT_G
Cuts at: 3207 3207
Size: 5608
MluI A'CGCG_T
Cuts at: 5484 5484
Size: 5608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2877 4722 4906 1305
Size: 1572 1845 184 2007
Fragments arranged by size:
2007 1845 1572 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3786
Fragments arranged by size:
3786 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3566 3925 4084 518
Size: 648 2400 359 159 2042
Fragments arranged by size:
2400 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2048 2339 3024 3701 4849 5094 1606
Size: 442 291 685 677 1148 245 2120
Fragments arranged by size:
2120 1148 685 677 442 291 245
NaeI GCC'GGC
Cuts at: 2402 2402
Size: 5608
NcoI C'CATG_G
Cuts at: 513 1065 2515 513
Size: 552 1450 3606
Fragments arranged by size:
3606 1450 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5608
NgoMIV G'CCGG_C
Cuts at: 2400 2400
Size: 5608
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5608
NotI GC'GGCC_GC
Cuts at: 3055 3055
Size: 5608
NspI r_CATG'y
Cuts at: 1132 1229 2966 4511 1132
Size: 97 1737 1545 2229
Fragments arranged by size:
2229 1737 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5608
PciI A'CATG_T
Cuts at: 4507 4507
Size: 5608
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5608
PmeI GTTT'AAAC
Cuts at: 2912 2912
Size: 5608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 5190 5222 3677
Size: 32 1481 32 4063
Fragments arranged by size:
4063 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2343 2343
Size: 5608
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5608
PsiI TTA'TAA
Cuts at: 3178 3178
Size: 5608
PstI C_TGCA'G
Cuts at: 830 2335 2960 830
Size: 1505 625 3478
Fragments arranged by size:
3478 1505 625
PvuI CG_AT'CG
Cuts at: 2001 2591 2001
Size: 590 5018
SacII CC_GC'GG
Cuts at: 2340 2340
Size: 5608
SalI G'TCGA_C
Cuts at: 1936 2950 1936
Size: 1014 4594
SbfI CC_TGCA'GG
Cuts at: 2960 2960
Size: 5608
ScaI AGT'ACT
Cuts at: 2530 3856 5568 2530
Size: 1326 1712 2570
Fragments arranged by size:
2570 1712 1326
SfcI C'TryA_G
Cuts at: 826 1045 2331 2405 2956 4091 4772 4963 826
Size: 219 1286 74 551 1135 681 191 1471
Fragments arranged by size:
1471 1286 1135 681 551 219 191 74
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5608
SmaI CCC'GGG
Cuts at: 1872 2935 1872
Size: 1063 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2172 2524 2764 3712 4613 4875
Size: 197 926 229 352 240 948 901 262
Cuts at: 4875 820
Size: 1553
Fragments arranged by size:
1553 948 926 901 352 262 240 229
197
SnaBI TAC'GTA
Cuts at: 493 3399 493
Size: 2906 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5608
SphI G_CATG'C
Cuts at: 1132 2966 1132
Size: 1834 3774
SspI AAT'ATT
Cuts at: 5 52 3532 5
Size: 47 3480 2081
Fragments arranged by size:
3480 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5608
StyI C'CwwG_G
Cuts at: 513 1065 2502 2515 3047 513
Size: 552 1437 13 532 3074
Fragments arranged by size:
3074 1437 552 532 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2230 3635 3652 3805 3990 4435 325
Size: 1255 650 1405 17 153 185 445 1498
Fragments arranged by size:
1498 1405 1255 650 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2074 2133 2528
Size: 274 80 33 51 1443 97 59 395
Cuts at: 2528 3854 5566 96
Size: 1326 1712 138
Fragments arranged by size:
1712 1443 1326 395 274 138 97 80
59 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2056 2088 5568 5600 2056
Size: 32 3480 32 2064
Fragments arranged by size:
3480 2064 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5608
XbaI T'CTAG_A
Cuts at: 2944 2944
Size: 5608
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2419 2419
Size: 5608
XhoI C'TCGA_G
Cuts at: 1943 2524 1943
Size: 581 5027
XmaI C'CCGG_G
Cuts at: 1870 2933 1870
Size: 1063 4545
XmnI GAAnn'nnTTC
Cuts at: 3737 3737
Size: 5608
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3416 276
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI
SfoI SrfI SwaI