################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BcgI BciVI BclI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmAI BsmBI BsmI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MaeII MamI MfeI MflI MluI MroI MseI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfuI SgrAI SnaBI SpeI Sse8387I SspI StyI SwaI TaqI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AcyI 248 GGCGCC G[GA]^CG[CT]C Aor51HI 432 AGCGCT AGC^GCT ApaI 235 GGGCCC GGGCC^C Asp700I 660 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 490 CCCGGG C^[CT]CG[GA]G BanII 235 GGGCCC G[GA]GC[CT]^C BbeI 248 GGCGCC GGCGC^C BbvCI 152 CCTCAGC CCTCAGC(-5/-2) BlpI 771 GCTGAGC GC^T[ACGT]AGC BpmI 515 CTCCAG CTGGAG(16/14) Bpu1102I 771 GCTGAGC GC^T[ACGT]AGC BsaHI 248 GGCGCC G[GA]^CG[CT]C BsiEI 129 CGGCCG CG[GA][CT]^CG BsoBI 490 CCCGGG C^[CT]CG[GA]G Bsp1407I 611 TGTACA T^GTACA BsrGI 611 TGTACA T^GTACA BsrI 506 CCAGT ACTGG(1/-1) BssSI 763 CTCGTG CACGAG(-5/-1) BstAPI 520 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 771 GCTGAGC GC^T[ACGT]AGC DpnII 309 GATC ^GATC EagI 129 CGGCCG C^GGCCG EarI 664 CTCTTC CTCTTC(1/4) EclXI 129 CGGCCG C^GGCCG Eco47III 432 AGCGCT AGC^GCT Eco52I 129 CGGCCG C^GGCCG HgaI 420 GCGTC GACGC(5/10) Hin1I 248 GGCGCC G[GA]^CG[CT]C HincII 865 GTCAAC GT[CT]^[GA]AC HindII 865 GTCAAC GT[CT]^[GA]AC HinfI 693 GAATC G^A[ACGT]TC HphI 306 GGTGA GGTGA(8/7) KasI 248 GGCGCC G^GCGCC Ksp632I 664 CTCTTC CTCTTC(1/4) MaeIII 323 GTTAC ^GT[ACGT]AC MboI 309 GATC ^GATC NarI 248 GGCGCC GG^CGCC NdeII 309 GATC ^GATC NspI 705 GCATGC [GA]CATG^[CT] PstI 898 CTGCAG CTGCA^G Sau3AI 309 GATC ^GATC SfcI 898 CTGCAG C^T[GA][CT]AG SfiI 227 GGCCCCAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 248 GGCGCC GGC^GCC SmaI 490 CCCGGG CCC^GGG SmlI 361 CTCAAG C^T[CT][GA]AG SphI 705 GCATGC GCATG^C SspBI 611 TGTACA T^GTACA TfiI 693 GAATC G^A[AT]TC XcmI 777 CCAGGCACTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 490 CCCGGG C^CCGGG XmaI 490 CCCGGG C^CCGGG XmnI 660 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AspHI 382 GTGCTC G[AT]GC[AT]^C 874 GTGCTC AvaII 56 GGACC G^G[AT]CC 330 GGACC BalI 923 TGGCCA TGG^CCA 944 TGGCCA BcnI 490 CCCGG CC^[GC]GG 491 CCGGG BfaI 559 CTAG C^TAG 697 CTAG BglI 138 GCCCTTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 228 GCCCCAGGGGC BseRI 150 CTCCTC GAGGAG(10/8) 571 CTCCTC BsiHKAI 382 GTGCTC G[AT]GC[AT]^C 874 GTGCTC BsmFI 38 GTCCC GGGAC(10/14) 509 GTCCC BspHI 626 TCATGA T^CATGA 809 TCATGA BsrDI 104 CATTGC GCAATG(2/0) 929 CATTGC BstF5I 12 GGATG GGATG(2/0) 751 GGATG FauI 50 CCCGC CCCGC(4/6) 454 GCGGG FokI 12 GGATG GGATG(9/13) 751 GGATG MaeI 559 CTAG C^TAG 697 CTAG MluNI 923 TGGCCA TGG^CCA 944 TGGCCA MscI 923 TGGCCA TGG^CCA 944 TGGCCA MslI 78 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 375 CACAGCTGTG NciI 490 CCCGG CC^[GC]GG 491 CCGGG PvuII 377 CAGCTG CAG^CTG 521 CAGCTG RcaI 626 TCATGA T^CATGA 809 TCATGA SexAI 302 ACCTGGT A^CC[AT]GGT 344 ACCAGGT SfaNI 63 GATGC GCATC(5/9) 827 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AfaI AluI AlwNI BanI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspA1I MspI MvaI MwoI NlaIII NlaIV RsaI Sau96I ScrFI StuI TseI TspRI