Restriction map for
pFN21AE6360
(Circular) (Six-base) MAPSORT of: pFN21AE6360.seq Check: 9110 from: 1 to: 5677
pFN21AE6360 5677 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3487 278
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3020 1937
Size: 1083 4594
Acc65I G'GTAC_C
Cuts at: 2998 2998
Size: 5677
AclI AA'CG_TT
Cuts at: 1563 3804 4177 1563
Size: 2241 373 3063
Fragments arranged by size:
3063 2241 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2306 2645 3740 5124 5392 1632
Size: 674 339 1095 1384 268 1917
Fragments arranged by size:
1917 1384 1095 674 339 268
AfeI AGC'GCT
Cuts at: 2439 2439
Size: 5677
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5480
AflIII A'CryG_T
Cuts at: 1203 4576 5553 1203
Size: 3373 977 1327
Fragments arranged by size:
3373 1327 977
AgeI A'CCGG_T
Cuts at: 4508 4508
Size: 5677
AhdI GACnn_n'nnGTC
Cuts at: 4406 4406
Size: 5677
AleI CACnn'nnGTG
Cuts at: 1166 2384 1166
Size: 1218 4459
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2402 1184
Size: 1218 4459
AlwNI CAG_nnn'CTG
Cuts at: 2356 2528 2531 2647 3420 4992 2356
Size: 172 3 116 773 1572 3041
Fragments arranged by size:
3041 1572 773 172 116 3
ApaI G_GGCC'C
Cuts at: 2244 2244
Size: 5677
ApaLI G'TGCA_C
Cuts at: 3734 4890 3734
Size: 1156 4521
ApoI r'AATT_y
Cuts at: 1493 2986 3212 3364 5473 1493
Size: 1493 226 152 2109 1697
Fragments arranged by size:
2109 1697 1493 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4231 160
Size: 872 3199 1606
Fragments arranged by size:
3199 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
AvaI C'yCGr_G
Cuts at: 1870 1943 2495 3002 1870
Size: 73 552 507 4545
Fragments arranged by size:
4545 552 507 73
BamHI G'GATC_C
Cuts at: 3007 3374 3007
Size: 367 5310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2096 2253 2409 2998 4453
Size: 325 217 627 309 157 156 589 1455
Cuts at: 4453 618
Size: 1842
Fragments arranged by size:
1842 1455 627 589 325 309 217 157
156
BanII G_rGCy'C
Cuts at: 1391 2244 2996 1391
Size: 853 752 4072
Fragments arranged by size:
4072 853 752
BbeI G_GCGC'C
Cuts at: 2257 2257
Size: 5677
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4706
BbvCI CC'TCA_GC
Cuts at: 2158 2158
Size: 5677
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3868 3902 1903
Size: 34 1931 34 3678
Fragments arranged by size:
3678 1931 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3569 4779 3569
Size: 1210 4467
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5677
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2149 2239 4288
Size: 107 122 71 631 605 477 90 2049
Cuts at: 4288 136
Size: 1525
Fragments arranged by size:
2049 1525 631 605 477 122 107 90
71
BglII A'GATC_T
Cuts at: 5672 5672
Size: 5677
BlpI GC'TnA_GC
Cuts at: 2777 3094 2777
Size: 317 5360
Bme1580I G_kGCm'C
Cuts at: 1790 2101 2145 2244 3738 4894 1790
Size: 311 44 99 1494 1156 2573
Fragments arranged by size:
2573 1494 1156 311 99 44
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5677
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2505 3408 4366
Size: 416 53 83 399 24 1088 903 958
Cuts at: 4366 442
Size: 1753
Fragments arranged by size:
1753 1088 958 903 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2505 4337 1239
Size: 1266 1832 2579
Fragments arranged by size:
2579 1832 1266
Bpu10I CC'TnA_GC
Cuts at: 2158 2158
Size: 5677
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2351 3802 4667 4965 2351
Size: 1451 865 298 3063
Fragments arranged by size:
3063 1451 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4340 882
Size: 617 2841 2219
Fragments arranged by size:
2841 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3468 493
Size: 711 2264 2702
Fragments arranged by size:
2702 2264 711
BsaBI GATnn'nnATC
Cuts at: 5671 5671
Size: 5677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2254 3484 3866 4501
Size: 53 83 186 936 721 1230 382 635
Cuts at: 4501 275
Size: 1451
Fragments arranged by size:
1451 1230 936 721 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4110 4508 4782 4929 5340 4110
Size: 398 274 147 411 4447
Fragments arranged by size:
4447 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5452
Fragments arranged by size:
5452 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2146 2567 1186
Size: 960 421 4296
Fragments arranged by size:
4296 960 421
BseYI C'CCAG_C
Cuts at: 2339 4880 5376 2339
Size: 2541 496 2640
Fragments arranged by size:
2640 2541 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5104
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2137 3127 3888 4916 1540
Size: 275 186 136 990 761 1028 2301
Fragments arranged by size:
2301 1028 990 761 275 186 136
BsiHKAI G_wGCw'C
Cuts at: 2391 2883 3738 3823 4894 2391
Size: 492 855 85 1071 3174
Fragments arranged by size:
3174 1071 855 492 85
BsmI GAATG_Cn'
Cuts at: 3188 3281 3188
Size: 93 5584
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 2145 2244 2391 2883 2996 3738
Size: 399 311 44 99 147 492 113 742
Cuts at: 3738 3823 4894 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 492 399 311 147 113
99 85 44
BspEI T'CCGG_A
Cuts at: 5340 5340
Size: 5677
BspHI T'CATG_A
Cuts at: 2631 2814 3566 5296 2631
Size: 183 752 1730 3012
Fragments arranged by size:
3012 1730 752 183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3032 844
Size: 2188 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3564 1394
Size: 429 1741 3507
Fragments arranged by size:
3507 1741 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2108 2933 4172 4346 65
Size: 1183 860 825 1239 174 1396
Fragments arranged by size:
1396 1239 1183 860 825 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4321 4508 1700
Size: 110 2511 187 2869
Fragments arranged by size:
2869 2511 187 110
BsrGI T'GTAC_A
Cuts at: 96 2616 96
Size: 2520 3157
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5677
BssSI C'ACGA_G
Cuts at: 2768 3737 4749 2768
Size: 969 1012 3696
Fragments arranged by size:
3696 1012 969
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2531 794
Size: 1737 3940
BstBI TT'CG_AA
Cuts at: 4481 4481
Size: 5677
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5677
BstYI r'GATC_y
Cuts at: 1972 3007 3374 3759 3776 5228 5314 5326 5672
Size: 1035 367 385 17 1452 86 12 346
Cuts at: 5672 1972
Size: 1977
Fragments arranged by size:
1977 1452 1035 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4938
Fragments arranged by size:
4938 683 56
BtsI GCAGTG_nn'
Cuts at: 2082 3193 3987 4007 2082
Size: 1111 794 20 3752
Fragments arranged by size:
3752 1111 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5642
DraI TTT'AAA
Cuts at: 2981 2981
Size: 5677
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4684 809
Size: 507 3368 1802
Fragments arranged by size:
3368 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2134 2928 2949
Size: 54 1425 50 275 18 304 794 21
Cuts at: 2949 3124 4013 5642 8
Size: 175 889 1629 43
Fragments arranged by size:
1629 1425 889 794 304 275 175 54
50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2134 3124 1537
Size: 275 322 990 4090
Fragments arranged by size:
4090 990 322 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2675 3607 1353
Size: 1322 932 3423
Fragments arranged by size:
3423 1322 932
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4264 4638 4784 387
Size: 1064 138 2675 374 146 1280
Fragments arranged by size:
2675 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2306 2505 2645 3740 4337 5124 5392
Size: 393 674 199 140 1095 597 787 268
Cuts at: 5392 1239
Size: 1524
Fragments arranged by size:
1524 1095 787 674 597 393 268 199
140
EcoO109I rG'GnC_Cy
Cuts at: 2240 2241 2482 2240
Size: 1 241 5435
Fragments arranged by size:
5435 241 1
EcoRI G'AATT_C
Cuts at: 2986 3364 2986
Size: 378 5299
FspI TGC'GCA
Cuts at: 4183 4183
Size: 5677
HaeII r_GCGC'y
Cuts at: 1678 2257 2298 2441 4824 1678
Size: 579 41 143 2383 2531
Fragments arranged by size:
2531 2383 579 143 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4318 4350 4392 4424 1373
Size: 32 135 32 2746 32 42 32 2626
Fragments arranged by size:
2746 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2872 3021 3267 669
Size: 988 281 934 149 246 3079
Fragments arranged by size:
3079 988 934 281 246 149
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5677
HpaI GTT'AAC
Cuts at: 3267 3267
Size: 5677
KasI G'GCGC_C
Cuts at: 2253 2253
Size: 5677
KpnI G_GTAC'C
Cuts at: 3002 3002
Size: 5677
MfeI C'AATT_G
Cuts at: 3276 3276
Size: 5677
MluI A'CGCG_T
Cuts at: 5553 5553
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4791 4975 1305
Size: 3486 184 2007
Fragments arranged by size:
3486 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2930 2951 10
Size: 54 1425 343 1098 21 2736
Fragments arranged by size:
2736 1425 1098 343 54 21
MslI CAynn'nnrTG
Cuts at: 518 1166 2087 2384 3635 3994 4153 518
Size: 648 921 297 1251 359 159 2042
Fragments arranged by size:
2042 1251 921 648 359 297 159
MspA1I CmG'CkG
Cuts at: 1606 2058 2384 2528 2644 3093 3770 4918 5163
Size: 452 326 144 116 449 677 1148 245
Cuts at: 5163 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 452 449 326 245 144
116
NarI GG'CG_CC
Cuts at: 2254 2254
Size: 5677
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5125
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5677
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5677
NotI GC'GGCC_GC
Cuts at: 3124 3124
Size: 5677
NspI r_CATG'y
Cuts at: 1132 1229 2714 3035 4580 1132
Size: 97 1485 321 1545 2229
Fragments arranged by size:
2229 1545 1485 321 97
PasI CC'CwG_GG
Cuts at: 1348 2236 1348
Size: 888 4789
PciI A'CATG_T
Cuts at: 4576 4576
Size: 5677
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5677
PfoI T'CCnGG_A
Cuts at: 2739 2739
Size: 5677
PmeI GTTT'AAAC
Cuts at: 2981 2981
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 5259 5291 3746
Size: 32 1481 32 4132
Fragments arranged by size:
4132 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5677
PsiI TTA'TAA
Cuts at: 3247 3247
Size: 5677
PspOMI G'GGCC_C
Cuts at: 2240 2240
Size: 5677
PstI C_TGCA'G
Cuts at: 830 2907 3029 830
Size: 2077 122 3478
Fragments arranged by size:
3478 2077 122
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5677
PvuII CAG'CTG
Cuts at: 2384 2528 2384
Size: 144 5533
SalI G'TCGA_C
Cuts at: 1936 3019 1936
Size: 1083 4594
SbfI CC_TGCA'GG
Cuts at: 3029 3029
Size: 5677
ScaI AGT'ACT
Cuts at: 3925 5637 3925
Size: 1712 3965
SexAI A'CCwGG_T
Cuts at: 2307 2349 2307
Size: 42 5635
SfcI C'TryA_G
Cuts at: 826 1045 2903 3025 4160 4841 5032 826
Size: 219 1858 122 1135 681 191 1471
Fragments arranged by size:
1858 1471 1135 681 219 191 122
SfiI GGCCn_nnn'nGGCC
Cuts at: 2239 2239
Size: 5677
SfoI GGC'GCC
Cuts at: 2255 2255
Size: 5677
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5677
SmaI CCC'GGG
Cuts at: 1872 2497 3004 1872
Size: 625 507 4545
Fragments arranged by size:
4545 625 507
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2366 3781 4682 4944 820
Size: 197 926 423 1415 901 262 1553
Fragments arranged by size:
1553 1415 926 901 423 262 197
SnaBI TAC'GTA
Cuts at: 493 3468 493
Size: 2975 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5677
SphI G_CATG'C
Cuts at: 1132 2714 3035 1132
Size: 1582 321 3774
Fragments arranged by size:
3774 1582 321
SspI AAT'ATT
Cuts at: 5 52 3601 5
Size: 47 3549 2081
Fragments arranged by size:
3549 2081 47
StuI AGG'CCT
Cuts at: 1517 2034 2568 2736 1517
Size: 517 534 168 4458
Fragments arranged by size:
4458 534 517 168
StyI C'CwwG_G
Cuts at: 513 1065 3116 513
Size: 552 2051 3074
Fragments arranged by size:
3074 2051 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3704 3721 3874 4059 4504 325
Size: 1255 2124 17 153 185 445 1498
Fragments arranged by size:
2124 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2586 2616 2961
Size: 274 80 33 51 1443 609 30 345
Cuts at: 2961 3923 5635 96
Size: 962 1712 138
Fragments arranged by size:
1712 1443 962 609 345 274 138 80
51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5637 5669 5637
Size: 32 5645
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5677
XbaI T'CTAG_A
Cuts at: 3013 3013
Size: 5677
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2789 2789
Size: 5677
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5677
XmaI C'CCGG_G
Cuts at: 1870 2495 3002 1870
Size: 625 507 4545
Fragments arranged by size:
4545 625 507
XmnI GAAnn'nnTTC
Cuts at: 2669 3806 2669
Size: 1137 4540
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3485 276
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SrfI SwaI