Restriction map for
pFN21AE5839
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# NO CUT ENZYMES ( 63 enzymes) #
##################################
AgeI Aor51HI ApaI AscI Asp700I
AviII AvrII BbeI BlnI BlpI
Bpu1102I BsiEI BsiWI BsmBI Bsp1407I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstXI BstZ17I CelII Cfr10I
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoRV FseI FspI
KasI KspI MluI NaeI NarI
NgoMIV NotI NruI NspV PacI
PinAI PmeI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI Sse8387I SspBI
SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 3398 GACGTC GACGT^C
AccIII 515 TCCGGA T^CCGGA
AcyI 3398 GACGTC G[GA]^CG[CT]C
AflII 2401 CTTAAG C^TTAAG
AspI 4986 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2034 TGGCCA TGG^CCA
BcgI 5592 GCAACAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2401 CTTAAG C^TTAAG
BglII 333 AGATCT A^GATCT
BsaBI 4542 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3398 GACGTC G[GA]^CG[CT]C
BseAI 515 TCCGGA T^CCGGA
BspDI 3108 ATCGAT AT^CGAT
BspEI 515 TCCGGA T^CCGGA
BssSI 6704 CACGAG CACGAG(-5/-1)
BstEII 6851 GGTTACC G^GT[ACGT]ACC
BstPI 6851 GGTTACC G^GT[ACGT]ACC
ClaI 3108 ATCGAT AT^CGAT
DraIII 3051 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2034 TGGCCA [CT]^GGCC[GA]
EcoO65I 6851 GGTTACC G^GT[ACGT]ACC
Hin1I 3398 GACGTC G[GA]^CG[CT]C
HpaI 1879 GTTAAC GTT^AAC
MamI 4542 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 7100 CAATTG C^AATTG
MluNI 2034 TGGCCA TGG^CCA
MroI 515 TCCGGA T^CCGGA
MscI 2034 TGGCCA TGG^CCA
MunI 7100 CAATTG C^AATTG
NdeI 4693 CATATG CA^TATG
NheI 1329 GCTAGC G^CTAGC
PflFI 4986 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3901 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 6179 GAGCTC GAGCT^C
SapI 2473 GAAGAGC GCTCTTC(1/4)
SpeI 6838 ACTAGT A^CTAGT
StuI 1232 AGGCCT AGG^CCT
Tth111I 4986 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 5389 GGTACC G^GTACC
6438 GGTACC
AccI 3412 GTAGAC GT^[AC][GT]AC
5735 GTCTAC
AccII 62 CGCG CG^CG
216 CGCG
AclI 5460 AACGTT AA^CGTT
7044 AACGTT
AhdI 292 GACGAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2593 GACACAGTGTC
Alw44I 542 GTGCAC G^TGCAC
1842 GTGCAC
ApaLI 542 GTGCAC G^TGCAC
1842 GTGCAC
AseI 3541 ATTAAT AT^TAAT
3646 ATTAAT
AsnI 3541 ATTAAT AT^TAAT
3646 ATTAAT
Asp718I 5389 GGTACC G^GTACC
6438 GGTACC
AspEI 292 GACGAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2593 GACACAGTGTC
BanII 3944 GAGCCC G[GA]GC[CT]^C
6179 GAGCTC
BbrPI 2147 CACGTG CAC^GTG
7581 CACGTG
BbsI 5311 GAAGAC GAAGAC(2/6)
2632 GTCTTC
BbvCI 7450 CCTCAGC CCTCAGC(-5/-2)
249 GCTGAGG
BglI 3981 GCCACTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5569 GCCCTGCTGGC
BpuAI 5311 GAAGAC GAAGAC(2/6)
2632 GTCTTC
BsaAI 2147 CACGTG [CT]AC^GT[GA]
7581 CACGTG
BsaI 3661 GAGACC GGTCTC(1/5)
5679 GAGACC
BsaWI 515 TCCGGA [AT]^CCGG[AT]
4067 ACCGGA
BstUI 62 CGCG CG^CG
216 CGCG
DraI 2710 TTTAAA TTT^AAA
4293 TTTAAA
Eam1105I 292 GACGAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2593 GACACAGTGTC
HindIII 491 AAGCTT A^AGCTT
632 AAGCTT
KpnI 5389 GGTACC GGTAC^C
6438 GGTACC
MvnI 62 CGCG CG^CG
216 CGCG
PaeR7I 3428 CTCGAG C^TCGAG
6392 CTCGAG
PflMI 2316 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6105 CCAGCCCATGG
PmaCI 2147 CACGTG CAC^GTG
7581 CACGTG
PmlI 2147 CACGTG CAC^GTG
7581 CACGTG
PpuMI 41 AGGACCT [GA]G^G[AT]CC[CT]
2288 AGGACCT
PshBI 3541 ATTAAT AT^TAAT
3646 ATTAAT
Psp1406I 5460 AACGTT AA^CGTT
7044 AACGTT
SmaI 1436 CCCGGG CCC^GGG
6174 CCCGGG
SphI 780 GCATGC GCATG^C
1683 GCATGC
Van91I 2316 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6105 CCAGCCCATGG
XhoI 3428 CTCGAG C^TCGAG
6392 CTCGAG
XmaCI 1436 CCCGGG C^CCGGG
6174 CCCGGG
XmaI 1436 CCCGGG C^CCGGG
6174 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BamHI BanI BbvI
BciVI BclI BcnI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspLU11I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI Eco81I EcoO109I EcoRI EcoRII
EcoT14I EcoT22I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PleI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII