Restriction map for
pFN21AE5839
(Linear) (Six-base) MAPSORT of: KIEE5839.seq Check: 48 from: 1 to: 7626
KIEE5839.orf
With 182 enzymes: SgfI *
December 5, 2014 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3181 4030 4750 5352 7626
Size: 3181 849 720 602 2274
Fragments arranged by size:
3181 2274 849 720 602
AatII G_ACGT'C
Cuts at: 0 3402 7626
Size: 3402 4224
AccI GT'mk_AC
Cuts at: 0 3413 5736 7626
Size: 3413 2323 1890
Fragments arranged by size:
3413 2323 1890
Acc65I G'GTAC_C
Cuts at: 0 5389 6438 7626
Size: 5389 1049 1188
Fragments arranged by size:
5389 1188 1049
AclI AA'CG_TT
Cuts at: 0 5461 7045 7626
Size: 5461 1584 581
Fragments arranged by size:
5461 1584 581
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1574 1814 2366 2836 3113 6524 6621 6795
Size: 1574 240 552 470 277 3411 97 174
Cuts at: 6795 7626
Size: 831
Fragments arranged by size:
3411 1574 831 552 470 277 240 174
97
AflII C'TTAA_G
Cuts at: 0 2401 7626
Size: 2401 5225
AflIII A'CryG_T
Cuts at: 0 1248 1347 2148 3603 7626
Size: 1248 99 801 1455 4023
Fragments arranged by size:
4023 1455 1248 801 99
AhdI GACnn_n'nnGTC
Cuts at: 0 297 2598 7626
Size: 297 2301 5028
Fragments arranged by size:
5028 2301 297
AleI CACnn'nnGTG
Cuts at: 0 2101 4576 7626
Size: 2101 2475 3050
Fragments arranged by size:
3050 2475 2101
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2183 4058 4361 5903 6165 7626
Size: 2183 1875 303 1542 262 1461
Fragments arranged by size:
2183 1875 1542 1461 303 262
AlwNI CAG_nnn'CTG
Cuts at: 0 505 1585 1774 2922 3796 4045 5773 5838
Size: 505 1080 189 1148 874 249 1728 65
Cuts at: 5838 6304 6958 7507 7626
Size: 466 654 549 119
Fragments arranged by size:
1728 1148 1080 874 654 549 505 466
249 189 119 65
ApaLI G'TGCA_C
Cuts at: 0 542 1842 7626
Size: 542 1300 5784
Fragments arranged by size:
5784 1300 542
ApoI r'AATT_y
Cuts at: 0 1108 2838 3916 4443 4842 4860 7124 7539
Size: 1108 1730 1078 527 399 18 2264 415
Cuts at: 7539 7626
Size: 87
Fragments arranged by size:
2264 1730 1108 1078 527 415 399 87
18
AseI AT'TA_AT
Cuts at: 0 3542 3647 7626
Size: 3542 105 3979
Fragments arranged by size:
3979 3542 105
AvaI C'yCGr_G
Cuts at: 0 1436 3428 6174 6392 7626
Size: 1436 1992 2746 218 1234
Fragments arranged by size:
2746 1992 1436 1234 218
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 6108 6141 7626
Size: 6108 33 1485
Fragments arranged by size:
6108 1485 33
BamHI G'GATC_C
Cuts at: 0 119 414 6462 7626
Size: 119 295 6048 1164
Fragments arranged by size:
6048 1164 295 119
BanI G'GyrC_C
Cuts at: 0 25 271 1870 2142 5176 5389 6438 6663
Size: 25 246 1599 272 3034 213 1049 225
Cuts at: 6663 6801 7429 7587 7626
Size: 138 628 158 39
Fragments arranged by size:
3034 1599 1049 628 272 246 225 213
158 138 39 25
BanII G_rGCy'C
Cuts at: 0 3948 6183 7626
Size: 3948 2235 1443
Fragments arranged by size:
3948 2235 1443
BbsI GAAGACnn'nnnn_
Cuts at: 0 2625 5318 7626
Size: 2625 2693 2308
Fragments arranged by size:
2693 2625 2308
BbvCI CC'TCA_GC
Cuts at: 0 250 7451 7626
Size: 250 7201 175
Fragments arranged by size:
7201 250 175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5581 5615 7626
Size: 5581 34 2011
Fragments arranged by size:
5581 2011 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1405 3134 3360 7626
Size: 1405 1729 226 4266
Fragments arranged by size:
4266 1729 1405 226
BclI T'GATC_A
Cuts at: 0 1902 2913 5225 7626
Size: 1902 1011 2312 2401
Fragments arranged by size:
2401 2312 1902 1011
BfrBI ATG'CAT
Cuts at: 0 780 934 4169 5416 7275 7608 7626
Size: 780 154 3235 1247 1859 333 18
Fragments arranged by size:
3235 1859 1247 780 333 154 18
BglI GCCn_nnn'nGGC
Cuts at: 0 3987 5575 7626
Size: 3987 1588 2051
Fragments arranged by size:
3987 2051 1588
BglII A'GATC_T
Cuts at: 0 333 7626
Size: 333 7293
Bme1580I G_kGCm'C
Cuts at: 0 546 1846 3023 5181 7590 7626
Size: 546 1300 1177 2158 2409 36
Fragments arranged by size:
2409 2158 1300 1177 546 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 340 894 2374 2770 3563 5524 5619 5668
Size: 340 554 1480 396 793 1961 95 49
Cuts at: 5668 6462 7626
Size: 794 1164
Fragments arranged by size:
1961 1480 1164 794 793 554 396 340
95 49
BmtI G_CTAG'C
Cuts at: 0 1333 7626
Size: 1333 6293
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 6078 7626
Size: 6078 1548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 962 2257 2431 2945 3512 3818 5402 5897
Size: 962 1295 174 514 567 306 1584 495
Cuts at: 5897 6167 7626
Size: 270 1459
Fragments arranged by size:
1584 1459 1295 962 567 514 495 306
270 174
Bpu10I CC'TnA_GC
Cuts at: 0 250 5300 5544 7451 7626
Size: 250 5050 244 1907 175
Fragments arranged by size:
5050 1907 250 244 175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1271 1496 1978 2111 3855 6011 6494 7626
Size: 1271 225 482 133 1744 2156 483 1132
Fragments arranged by size:
2156 1744 1271 1132 483 482 225 133
BsaI GGTCTCn'nnnn_
Cuts at: 0 3655 5673 7626
Size: 3655 2018 1953
Fragments arranged by size:
3655 2018 1953
BsaAI yAC'GTr
Cuts at: 0 2149 7583 7626
Size: 2149 5434 43
Fragments arranged by size:
5434 2149 43
BsaBI GATnn'nnATC
Cuts at: 0 4546 7626
Size: 4546 3080
BsaHI Gr'CG_yC
Cuts at: 0 3399 7626
Size: 3399 4227
BsaWI w'CCGG_w
Cuts at: 0 515 4067 7626
Size: 515 3552 3559
Fragments arranged by size:
3559 3552 515
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 935 965 1334 1364 3485 3515 3830 3860
Size: 935 30 369 30 2121 30 315 30
Cuts at: 3860 5844 5874 5895 5925 7127 7157 7626
Size: 1984 30 21 30 1202 30 469
Fragments arranged by size:
2121 1984 1202 935 469 369 315 30
30 30 30 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 2353 2642 4489 5153 5958 6250 6890
Size: 52 2301 289 1847 664 805 292 640
Cuts at: 6890 7626
Size: 736
Fragments arranged by size:
2301 1847 805 736 664 640 292 289
52
BseYI C'CCAG_C
Cuts at: 0 72 76 3529 4615 6582 6615 6680 6824
Size: 72 4 3453 1086 1967 33 65 144
Cuts at: 6824 7562 7626
Size: 738 64
Fragments arranged by size:
3453 1967 1086 738 144 72 65 64
33 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 1061 1103 1205 1870 1949 3209 4042
Size: 548 513 42 102 665 79 1260 833
Cuts at: 4042 4778 5957 6550 7626
Size: 736 1179 593 1076
Fragments arranged by size:
1260 1179 1076 833 736 665 593 548
513 102 79 42
BsiHKAI G_wGCw'C
Cuts at: 0 546 971 1125 1199 1287 1846 2620 3221
Size: 546 425 154 74 88 559 774 601
Cuts at: 3221 5433 6183 7626
Size: 2212 750 1443
Fragments arranged by size:
2212 1443 774 750 601 559 546 425
154 88 74
BsmI GAATG_Cn'
Cuts at: 0 4171 4284 6413 7555 7626
Size: 4171 113 2129 1142 71
Fragments arranged by size:
4171 2129 1142 113 71
Bsp1286I G_dGCh'C
Cuts at: 0 546 971 1125 1199 1287 1846 2620 3023
Size: 546 425 154 74 88 559 774 403
Cuts at: 3023 3221 3948 5181 5433 6183 7590 7626
Size: 198 727 1233 252 750 1407 36
Fragments arranged by size:
1407 1233 774 750 727 559 546 425
403 252 198 154 88 74 36
BspEI T'CCGG_A
Cuts at: 0 515 7626
Size: 515 7111
BspHI T'CATG_A
Cuts at: 0 879 3162 3198 7626
Size: 879 2283 36 4428
Fragments arranged by size:
4428 2283 879 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1033 3181 4030 4750 5352 6021 6312 7626
Size: 1033 2148 849 720 602 669 291 1314
Fragments arranged by size:
2148 1314 1033 849 720 669 602 291
BsrDI GCAATG_nn'
Cuts at: 0 442 3099 3221 3599 4993 7626
Size: 442 2657 122 378 1394 2633
Fragments arranged by size:
2657 2633 1394 442 378 122
BssSI C'ACGA_G
Cuts at: 0 6704 7626
Size: 6704 922
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1039 3232 4561 4799 7626
Size: 1039 2193 1329 238 2827
Fragments arranged by size:
2827 2193 1329 1039 238
BstEII G'GTnAC_C
Cuts at: 0 6851 7626
Size: 6851 775
BstYI r'GATC_y
Cuts at: 0 119 333 414 501 5292 6462 7503 7626
Size: 119 214 81 87 4791 1170 1041 123
Fragments arranged by size:
4791 1170 1041 214 123 119 87 81
Bsu36I CC'TnA_GG
Cuts at: 0 506 2690 4436 7626
Size: 506 2184 1746 3190
Fragments arranged by size:
3190 2184 1746 506
BtgI C'CryG_G
Cuts at: 0 5618 6110 6434 7626
Size: 5618 492 324 1192
Fragments arranged by size:
5618 1192 492 324
BtsI GCAGTG_nn'
Cuts at: 0 288 917 1217 4824 4879 5153 6533 7626
Size: 288 629 300 3607 55 274 1380 1093
Fragments arranged by size:
3607 1380 1093 629 300 288 274 55
ClaI AT'CG_AT
Cuts at: 0 3109 7626
Size: 3109 4517
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3077 3112 4524 4559 7028 7063 7626
Size: 3077 35 1412 35 2469 35 563
Fragments arranged by size:
3077 2469 1412 563 35 35 35
DraI TTT'AAA
Cuts at: 0 2712 4295 7626
Size: 2712 1583 3331
Fragments arranged by size:
3331 2712 1583
DraIII CAC_nnn'GTG
Cuts at: 0 3056 7626
Size: 3056 4570
EaeI y'GGCC_r
Cuts at: 0 2034 7626
Size: 2034 5592
EarI CTCTTCn'nnn_
Cuts at: 0 2468 3813 4319 4466 5130 5817 6897 7626
Size: 2468 1345 506 147 664 687 1080 729
Fragments arranged by size:
2468 1345 1080 729 687 664 506 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 57 86 221 7626
Size: 57 29 135 7405
Fragments arranged by size:
7405 135 57 29
EcoICRI GAG'CTC
Cuts at: 0 6181 7626
Size: 6181 1445
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 962 1574 1814 2257 2366 2431 2836 2945
Size: 962 612 240 443 109 65 405 109
Cuts at: 2945 3113 3512 3818 5402 5897 6167 6524 6621
Size: 168 399 306 1584 495 270 357 97
Cuts at: 6621 6795 7626
Size: 174 831
Fragments arranged by size:
1584 962 831 612 495 443 405 399
357 306 270 240 174 168 109 109
97 65
EcoO109I rG'GnC_Cy
Cuts at: 0 42 80 2289 7474 7626
Size: 42 38 2209 5185 152
Fragments arranged by size:
5185 2209 152 42 38
EcoRI G'AATT_C
Cuts at: 0 1108 4443 4860 7124 7626
Size: 1108 3335 417 2264 502
Fragments arranged by size:
3335 2264 1108 502 417
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 787 988 1826 2416 6052 7626
Size: 787 201 838 590 3636 1574
Fragments arranged by size:
3636 1574 838 787 590 201
HaeII r_GCGC'y
Cuts at: 0 223 1676 7016 7626
Size: 223 1453 5340 610
Fragments arranged by size:
5340 1453 610 223
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 283 295 315 327 880 912
Size: 34 32 217 12 20 12 553 32
Cuts at: 912 2584 2616 2725 2757 2826 2858 5135 5167
Size: 1672 32 109 32 69 32 2277 32
Cuts at: 5167 5589 5621 5895 5927 6076 6108 6454 6486
Size: 422 32 274 32 149 32 346 32
Cuts at: 6486 7626
Size: 1140
Fragments arranged by size:
2277 1672 1140 553 422 346 274 217
149 109 69 34 32 32 32 32
32 32 32 32 32 32 20 12
12
HincII GTy'rAC
Cuts at: 0 476 894 954 1881 2697 3135 4973 7623
Size: 476 418 60 927 816 438 1838 2650
Cuts at: 7623 7626
Size: 3
Fragments arranged by size:
2650 1838 927 816 476 438 418 60
3
HindIII A'AGCT_T
Cuts at: 0 491 632 7626
Size: 491 141 6994
Fragments arranged by size:
6994 491 141
HpaI GTT'AAC
Cuts at: 0 1881 7626
Size: 1881 5745
KpnI G_GTAC'C
Cuts at: 0 5393 6442 7626
Size: 5393 1049 1184
Fragments arranged by size:
5393 1184 1049
MfeI C'AATT_G
Cuts at: 0 7100 7626
Size: 7100 526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2042 3056 3282 3795 5458 5530 7164 7626
Size: 2042 1014 226 513 1663 72 1634 462
Fragments arranged by size:
2042 1663 1634 1014 513 462 226 72
MscI TGG'CCA
Cuts at: 0 2036 7626
Size: 2036 5590
MslI CAynn'nnrTG
Cuts at: 0 785 1103 1742 2042 2101 3384 3953 4013
Size: 785 318 639 300 59 1283 569 60
Cuts at: 4013 4223 4576 5186 7626
Size: 210 353 610 2440
Fragments arranged by size:
2440 1283 785 639 610 569 353 318
300 210 60 59
MspA1I CmG'CkG
Cuts at: 0 76 176 191 197 3004 6523 6544 7626
Size: 76 100 15 6 2807 3519 21 1082
Fragments arranged by size:
3519 2807 1082 100 76 21 15 6
NcoI C'CATG_G
Cuts at: 0 5618 6110 6434 7626
Size: 5618 492 324 1192
Fragments arranged by size:
5618 1192 492 324
NdeI CA'TA_TG
Cuts at: 0 4694 7626
Size: 4694 2932
NheI G'CTAG_C
Cuts at: 0 1329 7626
Size: 1329 6297
NsiI A_TGCA'T
Cuts at: 0 782 936 4171 5418 7277 7610 7626
Size: 782 154 3235 1247 1859 333 16
Fragments arranged by size:
3235 1859 1247 782 333 154 16
NspI r_CATG'y
Cuts at: 0 784 938 1252 1299 1351 1687 1928 3301
Size: 784 154 314 47 52 336 241 1373
Cuts at: 3301 3389 3607 6783 7608 7626
Size: 88 218 3176 825 18
Fragments arranged by size:
3176 1373 825 784 336 314 241 218
154 88 52 47 18
PciI A'CATG_T
Cuts at: 0 1248 1347 3603 7626
Size: 1248 99 2256 4023
Fragments arranged by size:
4023 2256 1248 99
PflMI CCAn_nnn'nTGG
Cuts at: 0 2322 6111 7626
Size: 2322 3789 1515
Fragments arranged by size:
3789 2322 1515
PfoI T'CCnGG_A
Cuts at: 0 7467 7626
Size: 7467 159
PmlI CAC'GTG
Cuts at: 0 2149 7583 7626
Size: 2149 5434 43
Fragments arranged by size:
5434 2149 43
PpuMI rG'GwC_Cy
Cuts at: 0 42 2289 7626
Size: 42 2247 5337
Fragments arranged by size:
5337 2247 42
PshAI GACnn'nnGTC
Cuts at: 0 3905 7626
Size: 3905 3721
PspXI vC'TCGA_Gb
Cuts at: 0 3428 6392 7626
Size: 3428 2964 1234
Fragments arranged by size:
3428 2964 1234
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3351 3383 4103 4135 7626
Size: 3351 32 720 32 3491
Fragments arranged by size:
3491 3351 720 32 32
PstI C_TGCA'G
Cuts at: 0 390 1305 2516 3615 4266 6528 7626
Size: 390 915 1211 1099 651 2262 1098
Fragments arranged by size:
2262 1211 1099 1098 915 651 390
PvuII CAG'CTG
Cuts at: 0 76 3004 6523 6544 7626
Size: 76 2928 3519 21 1082
Fragments arranged by size:
3519 2928 1082 76 21
SacI G_AGCT'C
Cuts at: 0 6183 7626
Size: 6183 1443
SapI GCTCTTCn'nnn_
Cuts at: 0 2468 7626
Size: 2468 5158
ScaI AGT'ACT
Cuts at: 0 384 4914 6021 7626
Size: 384 4530 1107 1605
Fragments arranged by size:
4530 1605 1107 384
SfcI C'TryA_G
Cuts at: 0 386 1301 1889 2512 3611 4262 4664 5816
Size: 386 915 588 623 1099 651 402 1152
Cuts at: 5816 6524 6713 7626
Size: 708 189 913
Fragments arranged by size:
1152 1099 915 913 708 651 623 588
402 386 189
SmaI CCC'GGG
Cuts at: 0 1438 6176 7626
Size: 1438 4738 1450
Fragments arranged by size:
4738 1450 1438
SmlI C'TyrA_G
Cuts at: 0 1286 1511 1993 2126 2401 3428 3870 6026
Size: 1286 225 482 133 275 1027 442 2156
Cuts at: 6026 6392 6509 7626
Size: 366 117 1117
Fragments arranged by size:
2156 1286 1117 1027 482 442 366 275
225 133 117
SpeI A'CTAG_T
Cuts at: 0 6838 7626
Size: 6838 788
SphI G_CATG'C
Cuts at: 0 784 1687 7626
Size: 784 903 5939
Fragments arranged by size:
5939 903 784
SspI AAT'ATT
Cuts at: 0 1657 2501 4126 7626
Size: 1657 844 1625 3500
Fragments arranged by size:
3500 1657 1625 844
StuI AGG'CCT
Cuts at: 0 1234 7626
Size: 1234 6392
StyI C'CwwG_G
Cuts at: 0 5618 6110 6434 7003 7626
Size: 5618 492 324 569 623
Fragments arranged by size:
5618 623 569 492 324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 100 2324 3749 5416 6090 6209 6342 7626
Size: 100 2224 1425 1667 674 119 133 1284
Fragments arranged by size:
2224 1667 1425 1284 674 133 119 100
TatI w'GTAC_w
Cuts at: 0 382 4912 6019 7626
Size: 382 4530 1107 1607
Fragments arranged by size:
4530 1607 1107 382
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 246 278 399 431 3260 3292 7626
Size: 246 32 121 32 2829 32 4334
Fragments arranged by size:
4334 2829 246 121 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4989 7626
Size: 4989 2637
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1614 2568 7508 7626
Size: 1614 954 4940 118
Fragments arranged by size:
4940 1614 954 118
XhoI C'TCGA_G
Cuts at: 0 3428 6392 7626
Size: 3428 2964 1234
Fragments arranged by size:
3428 2964 1234
XmaI C'CCGG_G
Cuts at: 0 1436 6174 7626
Size: 1436 4738 1452
Fragments arranged by size:
4738 1452 1436
ZraI GAC'GTC
Cuts at: 0 3400 7626
Size: 3400 4226
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AvaI
BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrDI BssSI BstAPI BstEII BstYI Bsu36I
BtgI BtsI ClaI CspCI DraI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MmeI MscI MslI
MspA1I NcoI NdeI NheI NsiI NspI PciI PflMI
PfoI PmlI PpuMI PshAI PspXI PsrI PstI PvuII
SacI SapI ScaI SfcI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AgeI AloI ApaI AscI AsiSI AvrII BbeI
BlpI BmgBI BsiEI BsiWI BsmBI BsrBI BsrFI BsrGI
BssHII BstBI BstXI BstZ17I BtgZI DrdI EagI EcoNI
EcoRV FseI FspI FspAI KasI MluI NaeI NarI
NgoMIV NotI NruI PacI PasI PmeI PpiI PsiI
PspOMI PvuI RsrII SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SnaBI SrfI SwaI XbaI
XmnI