Restriction map for
pFN21AE5840
(Circular) (Six-base) MAPSORT of: pFN21AE5840.seq Check: 6079 from: 1 to: 12556
pFN21AE5840 12556 bp
With 182 enzymes: SgfI *
December 8, 2014 13:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5186 6035 6755 7357 5186
Size: 849 720 602 10385
Fragments arranged by size:
10385 849 720 602
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5407 10366 278
Size: 53 83 186 936 3871 4959 2468
Fragments arranged by size:
4959 3871 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5418 7741 9899 1937
Size: 3481 2323 2158 4594
Fragments arranged by size:
4594 3481 2323 2158
Acc65I G'GTAC_C
Cuts at: 7394 8443 9877 7394
Size: 1049 1434 10073
Fragments arranged by size:
10073 1434 1049
AclI AA'CG_TT
Cuts at: 1563 7466 9050 10683 11056 1563
Size: 5903 1584 1633 373 3063
Fragments arranged by size:
5903 3063 1633 1584 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3579 3819 4371 4841 5118 8529 8626 8800
Size: 1947 240 552 470 277 3411 97 174
Cuts at: 8800 9573 9843 10619 12003 12271 1632
Size: 773 270 776 1384 268 1917
Fragments arranged by size:
3411 1947 1917 1384 776 773 552 470
277 270 268 240 174 97
AflII C'TTAA_G
Cuts at: 820 1017 4406 820
Size: 197 3389 8970
Fragments arranged by size:
8970 3389 197
AflIII A'CryG_T
Cuts at: 1203 3253 3352 4153 5608 11455 12432 1203
Size: 2050 99 801 1455 5847 977 1327
Fragments arranged by size:
5847 2050 1455 1327 977 801 99
AgeI A'CCGG_T
Cuts at: 11387 11387
Size: 12556
AhdI GACnn_n'nnGTC
Cuts at: 2302 4603 11285 2302
Size: 2301 6682 3573
Fragments arranged by size:
6682 3573 2301
AleI CACnn'nnGTG
Cuts at: 1166 4106 6581 1166
Size: 2940 2475 7141
Fragments arranged by size:
7141 2940 2475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4188 6063 6366 7908 8170 1184
Size: 3004 1875 303 1542 262 5570
Fragments arranged by size:
5570 3004 1875 1542 303 262
AlwNI CAG_nnn'CTG
Cuts at: 2510 3590 3779 4927 5801 6050 7778 7843 8309
Size: 1080 189 1148 874 249 1728 65 466
Cuts at: 8309 8963 9512 9596 10299 11871 2510
Size: 654 549 84 703 1572 3195
Fragments arranged by size:
3195 1728 1572 1148 1080 874 703 654
549 466 249 189 84 65
ApaLI G'TGCA_C
Cuts at: 2547 3847 10613 11769 2547
Size: 1300 6766 1156 3334
Fragments arranged by size:
6766 3334 1300 1156
ApoI r'AATT_y
Cuts at: 1493 3113 4843 5921 6448 6847 6865 9129 9544
Size: 1620 1730 1078 527 399 18 2264 415
Cuts at: 9544 9865 10091 10243 12352 1493
Size: 321 226 152 2109 1697
Fragments arranged by size:
2264 2109 1730 1697 1620 1078 527 415
399 321 226 152 18
AseI AT'TA_AT
Cuts at: 160 1032 5547 5652 11110 160
Size: 872 4515 105 5458 1606
Fragments arranged by size:
5458 4515 1606 872 105
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12556
AvaI C'yCGr_G
Cuts at: 1870 1943 3441 5433 8179 8397 9774 9852 9881
Size: 73 1498 1992 2746 218 1377 78 29
Cuts at: 9881 1870
Size: 4545
Fragments arranged by size:
4545 2746 1992 1498 1377 218 78 73
29
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 8113 8146 9840 9873 8113
Size: 33 1694 33 10796
Fragments arranged by size:
10796 1694 33 33
BamHI G'GATC_C
Cuts at: 2124 2419 8467 9886 10253 2124
Size: 295 6048 1419 367 4427
Fragments arranged by size:
6048 4427 1419 367 295
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2276 3875 4147 7181
Size: 325 217 627 243 246 1599 272 3034
Cuts at: 7181 7394 8443 8668 8806 9434 9684 9877 11332
Size: 213 1049 225 138 628 250 193 1455
Cuts at: 11332 618
Size: 1842
Fragments arranged by size:
3034 1842 1599 1455 1049 628 627 325
272 250 246 243 225 217 213 193
138
BanII G_rGCy'C
Cuts at: 1391 5953 8188 9875 1391
Size: 4562 2235 1687 4072
Fragments arranged by size:
4562 4072 2235 1687
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4630 7323 928
Size: 971 2731 2693 6161
Fragments arranged by size:
6161 2731 2693 971
BbvCI CC'TCA_GC
Cuts at: 2255 9456 9624 2255
Size: 7201 168 5187
Fragments arranged by size:
7201 5187 168
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 7586 7620 10747 10781 1903
Size: 34 50 34 5565 34 3127 34 3678
Fragments arranged by size:
5565 3678 3127 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3410 5139 5365 10448 11658 3410
Size: 1729 226 5083 1210 4308
Fragments arranged by size:
5083 4308 1729 1210 226
BclI T'GATC_A
Cuts at: 1548 3907 4918 7230 1548
Size: 2359 1011 2312 6874
Fragments arranged by size:
6874 2359 2312 1011
BfrBI ATG'CAT
Cuts at: 2785 2939 6174 7421 9280 2785
Size: 154 3235 1247 1859 6061
Fragments arranged by size:
6061 3235 1859 1247 154
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5992 7580 9572
Size: 107 122 71 631 605 4320 1588 1992
Cuts at: 9572 11167 136
Size: 1595 1525
Fragments arranged by size:
4320 1992 1595 1588 1525 631 605 122
107 71
BglII A'GATC_T
Cuts at: 2338 12551 2338
Size: 10213 2343
BlpI GC'TnA_GC
Cuts at: 9973 9973
Size: 12556
Bme1580I G_kGCm'C
Cuts at: 1790 2551 3851 5028 7186 10617 11773 1790
Size: 761 1300 1177 2158 3431 1156 2573
Fragments arranged by size:
3431 2573 2158 1300 1177 1156 761
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 12556
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2345 2899 4379
Size: 416 53 83 399 24 928 554 1480
Cuts at: 4379 4775 5568 7529 7624 7673 8467 10287 11245
Size: 396 793 1961 95 49 794 1820 958
Cuts at: 11245 442
Size: 1753
Fragments arranged by size:
1961 1820 1753 1480 958 928 794 793
554 416 399 396 95 83 53 49
24
BmtI G_CTAG'C
Cuts at: 1056 3338 1056
Size: 2282 10274
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8083 8083
Size: 12556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2967 4262 4436 4950 5517 5823 7407 7902
Size: 1728 1295 174 514 567 306 1584 495
Cuts at: 7902 8172 9738 9834 11216 1239
Size: 270 1566 96 1382 2579
Fragments arranged by size:
2579 1728 1584 1566 1382 1295 567 514
495 306 270 174 96
Bpu10I CC'TnA_GC
Cuts at: 2255 7305 7549 9456 9624 2255
Size: 5050 244 1907 168 5187
Fragments arranged by size:
5187 5050 1907 244 168
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3276 3501 3983 4116 5860 8016 8499 10681 11546
Size: 225 482 133 1744 2156 483 2182 865
Cuts at: 11546 11844 3276
Size: 298 3988
Fragments arranged by size:
3988 2182 2156 1744 865 483 482 298
225 133
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5660 7678 11219 882
Size: 617 4161 2018 3541 2219
Fragments arranged by size:
4161 3541 2219 2018 617
BsaAI yAC'GTr
Cuts at: 493 1204 4154 10347 493
Size: 711 2950 6193 2702
Fragments arranged by size:
6193 2950 2702 711
BsaBI GATnn'nnATC
Cuts at: 6551 12550 6551
Size: 5999 6557
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5404 10363 10745 11380
Size: 53 83 186 936 3871 4959 382 635
Cuts at: 11380 275
Size: 1451
Fragments arranged by size:
4959 3871 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2520 6072 10989 11387 11661 11808 12219 2520
Size: 3552 4917 398 274 147 411 2857
Fragments arranged by size:
4917 3552 2857 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2940 2970 3339 3369 5490
Size: 30 165 30 1537 30 369 30 2121
Cuts at: 5490 5520 5835 5865 7849 7879 7900 7930 9132
Size: 30 315 30 1984 30 21 30 1202
Cuts at: 9132 9162 9773 9803 1178
Size: 30 611 30 3931
Fragments arranged by size:
3931 2121 1984 1537 1202 611 369 315
165 30 30 30 30 30 30 30
30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 4358 4647 6494 7158 7963 8255 8895
Size: 871 2301 289 1847 664 805 292 640
Cuts at: 8895 9778 9831 1186
Size: 883 53 3911
Fragments arranged by size:
3911 2301 1847 883 871 805 664 640
292 289 53
BseYI C'CCAG_C
Cuts at: 2077 2081 5534 6620 8587 8620 8685 8829 9567
Size: 4 3453 1086 1967 33 65 144 738
Cuts at: 9567 11759 12255 2077
Size: 2192 496 2378
Fragments arranged by size:
3453 2378 2192 1967 1086 738 496 144
65 33 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2553 3066 3108 3210 3875 3954 5214
Size: 573 817 513 42 102 665 79 1260
Cuts at: 5214 6047 6783 7962 8555 1163
Size: 833 736 1179 593 5164
Fragments arranged by size:
5164 1260 1179 833 817 736 665 593
573 513 102 79 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 9659 9704 10006 10767 11795 1540
Size: 275 186 7658 45 302 761 1028 2301
Fragments arranged by size:
7658 2301 1028 761 302 275 186 45
BsiHKAI G_wGCw'C
Cuts at: 2551 2976 3130 3204 3292 3851 4625 5226 7438
Size: 425 154 74 88 559 774 601 2212
Cuts at: 7438 8188 10617 10702 11773 2551
Size: 750 2429 85 1071 3334
Fragments arranged by size:
3334 2429 2212 1071 774 750 601 559
425 154 88 85 74
BsmI GAATG_Cn'
Cuts at: 6176 6289 8418 9560 10067 10160 6176
Size: 113 2129 1142 507 93 8572
Fragments arranged by size:
8572 2129 1142 507 113 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2551 2976 3130 3204 3292 3851 4625
Size: 399 761 425 154 74 88 559 774
Cuts at: 4625 5028 5226 5953 7186 7438 8188 9875 10617
Size: 403 198 727 1233 252 750 1687 742
Cuts at: 10617 10702 11773 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1687 1233 1071 774 761 750 742
727 559 425 403 399 252 198 154
88 85 74
BspEI T'CCGG_A
Cuts at: 2520 12219 2520
Size: 9699 2857
BspHI T'CATG_A
Cuts at: 2884 5167 5203 10445 12175 2884
Size: 2283 36 5242 1730 3265
Fragments arranged by size:
5242 3265 2283 1730 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3038 5186 6035 6755 7357 8026 8317 9911
Size: 2194 2148 849 720 602 669 291 1594
Cuts at: 9911 844
Size: 3489
Fragments arranged by size:
3489 2194 2148 1594 849 720 669 602
291
BsrBI CCG'CTC
Cuts at: 1394 1823 10443 1394
Size: 429 8620 3507
Fragments arranged by size:
8620 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2447 5104 5226 5604 6998 9736 11051
Size: 1183 1199 2657 122 378 1394 2738 1315
Cuts at: 11051 11225 65
Size: 174 1396
Fragments arranged by size:
2738 2657 1396 1394 1315 1199 1183 378
174 122
BsrFI r'CCGG_y
Cuts at: 1700 1810 11200 11387 1700
Size: 110 9390 187 2869
Fragments arranged by size:
9390 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 12556
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12556
BssSI C'ACGA_G
Cuts at: 8709 10616 11628 8709
Size: 1907 1012 9637
Fragments arranged by size:
9637 1907 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3044 5237 6566 6804 794
Size: 2250 2193 1329 238 6546
Fragments arranged by size:
6546 2250 2193 1329 238
BstBI TT'CG_AA
Cuts at: 11360 11360
Size: 12556
BstEII G'GTnAC_C
Cuts at: 1184 8856 1184
Size: 7672 4884
BstYI r'GATC_y
Cuts at: 1972 2124 2338 2419 2506 7297 8467 9508 9886
Size: 152 214 81 87 4791 1170 1041 378
Cuts at: 9886 10253 10638 10655 12107 12193 12205 12551 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
4791 1977 1452 1170 1041 385 378 367
346 214 152 87 86 81 17 12
Bsu36I CC'TnA_GG
Cuts at: 2511 4695 6441 2511
Size: 2184 1746 8626
Fragments arranged by size:
8626 2184 1746
BtgI C'CryG_G
Cuts at: 513 1065 4176 7623 8115 8439 513
Size: 552 3111 3447 492 324 4630
Fragments arranged by size:
4630 3447 3111 552 492 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 11817
Fragments arranged by size:
11817 683 56
BtsI GCAGTG_nn'
Cuts at: 2293 2922 3222 6829 6884 7158 8538 10072 10866
Size: 629 300 3607 55 274 1380 1534 794
Cuts at: 10866 10886 2293
Size: 20 3963
Fragments arranged by size:
3963 3607 1534 1380 794 629 300 274
55 20
ClaI AT'CG_AT
Cuts at: 1554 5114 1554
Size: 3560 8996
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5082 5117 6529 6564 9033 9068 529
Size: 35 4518 35 1412 35 2469 35 4017
Fragments arranged by size:
4518 4017 2469 1412 35 35 35 35
DraI TTT'AAA
Cuts at: 4717 6300 9860 4717
Size: 1583 3560 7413
Fragments arranged by size:
7413 3560 1583
DraIII CAC_nnn'GTG
Cuts at: 5061 5061
Size: 12556
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 11563 809
Size: 507 10247 1802
Fragments arranged by size:
10247 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4039 9656 10003
Size: 54 1425 50 275 18 2209 5617 347
Cuts at: 10003 10892 12521 8
Size: 889 1629 43
Fragments arranged by size:
5617 2209 1629 1425 889 347 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 9656 10003 1537
Size: 275 7844 347 4090
Fragments arranged by size:
7844 4090 347 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4473 5818 6324 6471 7135 7822 8902 9638
Size: 3120 1345 506 147 664 687 1080 736
Cuts at: 9638 9838 10486 1353
Size: 200 648 3423
Fragments arranged by size:
3423 3120 1345 1080 736 687 664 648
506 200 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2062 2091 2226 11143 11517 11663
Size: 1064 138 473 29 135 8917 374 146
Cuts at: 11663 387
Size: 1280
Fragments arranged by size:
8917 1280 1064 473 374 146 138 135
29
EcoICRI GAG'CTC
Cuts at: 8186 8186
Size: 12556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2967 3579 3819 4262 4371 4436 4841
Size: 393 1335 612 240 443 109 65 405
Cuts at: 4841 4950 5118 5517 5823 7407 7902 8172 8529
Size: 109 168 399 306 1584 495 270 357
Cuts at: 8529 8626 8800 9573 9738 9834 9843 10619 11216
Size: 97 174 773 165 96 9 776 597
Cuts at: 11216 12003 12271 1239
Size: 787 268 1524
Fragments arranged by size:
1584 1524 1335 787 776 773 612 597
495 443 405 399 393 357 306 270
268 240 174 168 165 109 109 97
96 65 9
EcoO109I rG'GnC_Cy
Cuts at: 2047 2085 4294 9479 2047
Size: 38 2209 5185 5124
Fragments arranged by size:
5185 5124 2209 38
EcoRI G'AATT_C
Cuts at: 3113 6448 6865 9129 9865 10243 3113
Size: 3335 417 2264 736 378 5426
Fragments arranged by size:
5426 3335 2264 736 417 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2792 2993 3831 4421 8057 2792
Size: 201 838 590 3636 7291
Fragments arranged by size:
7291 3636 838 590 201
FspI TGC'GCA
Cuts at: 11062 11062
Size: 12556
HaeII r_GCGC'y
Cuts at: 1678 2228 3681 9021 11703 1678
Size: 550 1453 5340 2682 2531
Fragments arranged by size:
5340 2682 2531 1453 550
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 2288 2300 2320
Size: 32 135 32 467 32 217 12 20
Cuts at: 2320 2332 2885 2917 4589 4621 4730 4762 4831
Size: 12 553 32 1672 32 109 32 69
Cuts at: 4831 4863 7140 7172 7594 7626 7900 7932 8081
Size: 32 2277 32 422 32 274 32 149
Cuts at: 8081 8113 8459 8491 11197 11229 11271 11303 1373
Size: 32 346 32 2706 32 42 32 2626
Fragments arranged by size:
2706 2626 2277 1672 553 467 422 346
274 217 149 135 109 69 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2481 2899 2959 3886 4702 5140
Size: 988 281 543 418 60 927 816 438
Cuts at: 5140 6978 9900 10146 669
Size: 1838 2922 246 3079
Fragments arranged by size:
3079 2922 1838 988 927 816 543 438
418 281 246 60
HindIII A'AGCT_T
Cuts at: 748 2496 2637 748
Size: 1748 141 10667
Fragments arranged by size:
10667 1748 141
HpaI GTT'AAC
Cuts at: 3886 10146 3886
Size: 6260 6296
KpnI G_GTAC'C
Cuts at: 7398 8447 9881 7398
Size: 1049 1434 10073
Fragments arranged by size:
10073 1434 1049
MfeI C'AATT_G
Cuts at: 9105 10155 9105
Size: 1050 11506
MluI A'CGCG_T
Cuts at: 12432 12432
Size: 12556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4047 5061 5287 5800 7463 7535 9169 9816
Size: 2742 1014 226 513 1663 72 1634 647
Cuts at: 9816 11670 11854 1305
Size: 1854 184 2007
Fragments arranged by size:
2742 2007 1854 1663 1634 1014 647 513
226 184 72
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4041 10
Size: 54 1425 343 2209 8525
Fragments arranged by size:
8525 2209 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2790 3108 3747 4047 4106 5389 5958
Size: 648 1624 318 639 300 59 1283 569
Cuts at: 5958 6018 6228 6581 7191 10514 10873 11032 518
Size: 60 210 353 610 3323 359 159 2042
Fragments arranged by size:
3323 2042 1624 1283 648 639 610 569
359 353 318 300 210 159 60 59
MspA1I CmG'CkG
Cuts at: 1606 2081 2181 2196 2202 5009 8528 8549 9972
Size: 475 100 15 6 2807 3519 21 1423
Cuts at: 9972 10649 11797 12042 1606
Size: 677 1148 245 2120
Fragments arranged by size:
3519 2807 2120 1423 1148 677 475 245
100 21 15 6
NcoI C'CATG_G
Cuts at: 513 1065 4176 7623 8115 8439 513
Size: 552 3111 3447 492 324 4630
Fragments arranged by size:
4630 3447 3111 552 492 324
NdeI CA'TA_TG
Cuts at: 387 6699 387
Size: 6312 6244
NheI G'CTAG_C
Cuts at: 1052 3334 1052
Size: 2282 10274
NotI GC'GGCC_GC
Cuts at: 10003 10003
Size: 12556
NsiI A_TGCA'T
Cuts at: 2787 2941 6176 7423 9282 2787
Size: 154 3235 1247 1859 6061
Fragments arranged by size:
6061 3235 1859 1247 154
NspI r_CATG'y
Cuts at: 1132 1229 2789 2943 3257 3304 3356 3692 3933
Size: 97 1560 154 314 47 52 336 241
Cuts at: 3933 5306 5394 5612 8788 9914 11459 1132
Size: 1373 88 218 3176 1126 1545 2229
Fragments arranged by size:
3176 2229 1560 1545 1373 1126 336 314
241 218 154 97 88 52 47
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 12556
PciI A'CATG_T
Cuts at: 3253 3352 5608 11455 3253
Size: 99 2256 5847 4354
Fragments arranged by size:
5847 4354 2256 99
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4327 8116 1108
Size: 3219 3789 5548
Fragments arranged by size:
5548 3789 3219
PfoI T'CCnGG_A
Cuts at: 9472 9472
Size: 12556
PmeI GTTT'AAAC
Cuts at: 9860 9860
Size: 12556
PmlI CAC'GTG
Cuts at: 4154 4154
Size: 12556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10625 10657 12138 12170 10625
Size: 32 1481 32 11011
Fragments arranged by size:
11011 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2047 4294 2047
Size: 2247 10309
PshAI GACnn'nnGTC
Cuts at: 1613 5910 1613
Size: 4297 8259
PsiI TTA'TAA
Cuts at: 10126 10126
Size: 12556
PspXI vC'TCGA_Gb
Cuts at: 5433 8397 5433
Size: 2964 9592
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5356 5388 6108 6140 5356
Size: 32 720 32 11772
Fragments arranged by size:
11772 720 32 32
PstI C_TGCA'G
Cuts at: 830 2395 3310 4521 5620 6271 8533 9908 830
Size: 1565 915 1211 1099 651 2262 1375 3478
Fragments arranged by size:
3478 2262 1565 1375 1211 1099 915 651
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12556
PvuII CAG'CTG
Cuts at: 2081 5009 8528 8549 2081
Size: 2928 3519 21 6088
Fragments arranged by size:
6088 3519 2928 21
SacI G_AGCT'C
Cuts at: 8188 8188
Size: 12556
SalI G'TCGA_C
Cuts at: 1936 9898 1936
Size: 7962 4594
SapI GCTCTTCn'nnn_
Cuts at: 4473 4473
Size: 12556
SbfI CC_TGCA'GG
Cuts at: 9908 9908
Size: 12556
ScaI AGT'ACT
Cuts at: 2389 6919 8026 10804 12516 2389
Size: 4530 1107 2778 1712 2429
Fragments arranged by size:
4530 2778 2429 1712 1107
SfcI C'TryA_G
Cuts at: 826 1045 2391 3306 3894 4517 5616 6267 6669
Size: 219 1346 915 588 623 1099 651 402
Cuts at: 6669 7821 8529 8718 9904 11039 11720 11911 826
Size: 1152 708 189 1186 1135 681 191 1471
Fragments arranged by size:
1471 1346 1186 1152 1135 1099 915 708
681 651 623 588 402 219 191 189
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12556
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12556
SmaI CCC'GGG
Cuts at: 1872 3443 8181 9776 9883 1872
Size: 1571 4738 1595 107 4545
Fragments arranged by size:
4738 4545 1595 1571 107
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3291 3516 3998 4131 4406 5433
Size: 197 926 1348 225 482 133 275 1027
Cuts at: 5433 5875 8031 8397 8514 10660 11561 11823 820
Size: 442 2156 366 117 2146 901 262 1553
Fragments arranged by size:
2156 2146 1553 1348 1027 926 901 482
442 366 275 262 225 197 133 117
SnaBI TAC'GTA
Cuts at: 493 10347 493
Size: 9854 2702
SpeI A'CTAG_T
Cuts at: 152 8843 152
Size: 8691 3865
SphI G_CATG'C
Cuts at: 1132 2789 3692 9914 1132
Size: 1657 903 6222 3774
Fragments arranged by size:
6222 3774 1657 903
SspI AAT'ATT
Cuts at: 5 52 3662 4506 6131 10480 5
Size: 47 3610 844 1625 4349 2081
Fragments arranged by size:
4349 3610 2081 1625 844 47
StuI AGG'CCT
Cuts at: 1517 3239 1517
Size: 1722 10834
StyI C'CwwG_G
Cuts at: 513 1065 4176 7623 8115 8439 9008 9995 513
Size: 552 3111 3447 492 324 569 987 3074
Fragments arranged by size:
3447 3111 3074 987 569 552 492 324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2105 4329 5754 7421 8095 8214 8347
Size: 1255 525 2224 1425 1667 674 119 133
Cuts at: 8347 9652 9718 9749 10583 10600 10753 10938 11383
Size: 1305 66 31 834 17 153 185 445
Cuts at: 11383 325
Size: 1498
Fragments arranged by size:
2224 1667 1498 1425 1305 1255 834 674
525 445 185 153 133 119 66 31
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2387 6917 8024
Size: 274 80 33 51 1443 410 4530 1107
Cuts at: 8024 10802 12514 96
Size: 2778 1712 138
Fragments arranged by size:
4530 2778 1712 1443 1107 410 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2251 2283 2404 2436 5265 5297 9773 9805 12516
Size: 32 121 32 2829 32 4476 32 2711
Cuts at: 12516 12548 2251
Size: 32 2259
Fragments arranged by size:
4476 2829 2711 2259 121 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6994 1316
Size: 5678 6878
XbaI T'CTAG_A
Cuts at: 9892 9892
Size: 12556
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3619 4573 9513 3619
Size: 954 4940 6662
Fragments arranged by size:
6662 4940 954
XhoI C'TCGA_G
Cuts at: 1943 5433 8397 1943
Size: 3490 2964 6102
Fragments arranged by size:
6102 3490 2964
XmaI C'CCGG_G
Cuts at: 1870 3441 8179 9774 9881 1870
Size: 1571 4738 1595 107 4545
Fragments arranged by size:
4738 4545 1595 1571 107
XmnI GAAnn'nnTTC
Cuts at: 10685 10685
Size: 12556
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5405 10364 276
Size: 53 83 186 936 3871 4959 2468
Fragments arranged by size:
4959 3871 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BsiWI BsmBI
BstXI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PspOMI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI