################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII BamHI BbeI BbrPI BbvCI BclI BfrI BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII Eam1105I Eco47III Eco81I EcoNI EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KspI MaeII MamI MfeI MluI MroI MseI MslI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### Acc65I 554 GGTACC G^GTACC AfaI 555 GTAC GT^AC Asp718I 554 GGTACC G^GTACC AvaI 405 CCCGGG C^[CT]CG[GA]G AvrII 156 CCTAGG C^CTAGG BalI 248 TGGCCA TGG^CCA BanI 554 GGTACC G^G[CT][GA]CC BbsI 323 GTCTTC GAAGAC(2/6) BcgI 43 GCAGTTCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 133 GTATCC GTATCC(6/5) BglI 490 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 201 AGATCT A^GATCT BlnI 156 CCTAGG C^CTAGG BlpI 435 GCTGAGC GC^T[ACGT]AGC BpmI 76 CTGGAG CTGGAG(16/14) Bpu1102I 435 GCTGAGC GC^T[ACGT]AGC BpuAI 323 GTCTTC GAAGAC(2/6) BsaWI 173 ACCGGA [AT]^CCGG[AT] BsiEI 165 CGGCCG CG[GA][CT]^CG BsmI 358 GAATGC GAATGC(1/-1) BsoBI 405 CCCGGG C^[CT]CG[GA]G BsrBI 79 GAGCGG CCGCTC(-3/-3) BstEII 409 GGTTACC G^GT[ACGT]ACC BstPI 409 GGTTACC G^GT[ACGT]ACC BstYI 201 AGATCT [GA]^GATC[CT] Cac8I 96 GCTAGC GC[ACGT]^[ACGT]GC CelII 435 GCTGAGC GC^T[ACGT]AGC DdeI 436 CTGAG C^T[ACGT]AG DpnII 202 GATC ^GATC DraII 314 GGGACCC [GA]G^G[ACGT]CC[CT] DrdI 172 GACCGGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 165 CGGCCG C^GGCCG EclXI 165 CGGCCG C^GGCCG Eco52I 165 CGGCCG C^GGCCG EcoO109I 314 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 409 GGTTACC G^GT[ACGT]ACC HinfI 218 GAGTC G^A[ACGT]TC HphI 60 GGTGA GGTGA(8/7) KpnI 554 GGTACC GGTAC^C MboI 202 GATC ^GATC MflI 201 AGATCT [GA]^GATC[CT] MluNI 248 TGGCCA TGG^CCA MscI 248 TGGCCA TGG^CCA MspA1I 145 CAGCTG C[AC]G^C[GT]G MwoI 490 GCCGTGGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 202 GATC ^GATC NheI 96 GCTAGC G^CTAGC PleI 218 GAGTC GAGTC(4/5) PpuMI 314 GGGACCC [GA]G^G[AT]CC[CT] PshAI 316 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 41 CTGCAG CTGCA^G PvuII 145 CAGCTG CAG^CTG RsaI 555 GTAC GT^AC SacI 376 GAGCTC GAGCT^C Sau3AI 202 GATC ^GATC SfcI 41 CTGCAG C^T[GA][CT]AG SmaI 405 CCCGGG CCC^GGG Tsp509I 616 AATT ^AATT XcmI 482 CCATCCTGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 201 AGATCT [GA]^GATC[CT] XmaCI 405 CCCGGG C^CCGGG XmaI 405 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AciI 81 GCGG CCGC(-3/-1) 164 GCGG AspHI 268 GAGCAC G[AT]GC[AT]^C 376 GAGCTC BanII 376 GAGCTC G[GA]GC[CT]^C 383 GGGCTC BseRI 265 GAGGAG GAGGAG(10/8) 566 GAGGAG BsiHKAI 268 GAGCAC G[AT]GC[AT]^C 376 GAGCTC BsmFI 170 GGGAC GGGAC(10/14) 314 GGGAC BstF5I 348 CATCC GGATG(2/0) 483 CATCC BstNI 75 CCTGG CC^[AT]GG 486 CCTGG EarI 309 GAAGAG CTCTTC(1/4) 589 GAAGAG EcoRII 75 CCTGG ^CC[AT]GG 486 CCTGG FokI 348 CATCC GGATG(9/13) 483 CATCC Ksp632I 309 GAAGAG CTCTTC(1/4) 589 GAAGAG MvaI 75 CCTGG CC^[AT]GG 486 CCTGG NcoI 351 CCATGG C^CATGG 414 CCATGG SfaNI 347 GCATC GCATC(5/9) 395 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AluI AvaII BbvI BcnI BfaI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI Cfr13I DsaI EaeI EcoT14I Fnu4HI HaeIII HapII HpaII ItaI MaeI MaeIII MboII MnlI MspI NciI NlaIII NlaIV Sau96I ScrFI StyI TseI Tsp45I TspRI