(Circular) (Six-base) MAPSORT of: pFN21AE5270.seq Check: 7976 from: 1 to: 5329 pFN21AE5270 5329 bp With 182 enzymes: SgfI * December 8, 2014 13:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3139 278 Size: 53 83 186 936 1603 2468 Fragments arranged by size: 2468 1603 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2672 1937 Size: 735 4594 Acc65I G'GTAC_C Cuts at: 2559 2650 2559 Size: 91 5238 AclI AA'CG_TT Cuts at: 1563 3456 3829 1563 Size: 1893 373 3063 Fragments arranged by size: 3063 1893 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2051 3392 4776 5044 1632 Size: 419 1341 1384 268 1917 Fragments arranged by size: 1917 1384 1341 419 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5132 AflIII A'CryG_T Cuts at: 1203 4228 5205 1203 Size: 3025 977 1327 Fragments arranged by size: 3025 1327 977 AgeI A'CCGG_T Cuts at: 4160 4160 Size: 5329 AhdI GACnn_n'nnGTC Cuts at: 4058 4058 Size: 5329 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5329 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5329 AlwNI CAG_nnn'CTG Cuts at: 3072 4644 3072 Size: 1572 3757 ApaLI G'TGCA_C Cuts at: 3386 4542 3386 Size: 1156 4173 ApoI r'AATT_y Cuts at: 1493 2638 2864 3016 5125 1493 Size: 1145 226 152 2109 1697 Fragments arranged by size: 2109 1697 1145 226 152 AseI AT'TA_AT Cuts at: 160 1032 3883 160 Size: 872 2851 1606 Fragments arranged by size: 2851 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5329 AvaI C'yCGr_G Cuts at: 1870 1943 2410 2654 1870 Size: 73 467 244 4545 Fragments arranged by size: 4545 467 244 73 AvrII C'CTAG_G Cuts at: 2161 2161 Size: 5329 BamHI G'GATC_C Cuts at: 2659 3026 2659 Size: 367 4962 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2559 2650 4105 618 Size: 325 217 627 772 91 1455 1842 Fragments arranged by size: 1842 1455 772 627 325 217 91 BanII G_rGCy'C Cuts at: 1391 2385 2392 2648 1391 Size: 994 7 256 4072 Fragments arranged by size: 4072 994 256 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2321 928 Size: 971 422 3936 Fragments arranged by size: 3936 971 422 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2037 2071 3520 3554 1903 Size: 34 100 34 1449 34 3678 Fragments arranged by size: 3678 1449 100 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2149 3221 4431 2149 Size: 1072 1210 3047 Fragments arranged by size: 3047 1210 1072 BclI T'GATC_A Cuts at: 1548 1548 Size: 5329 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2501 3940 136 Size: 107 122 71 631 605 829 1439 1525 Fragments arranged by size: 1525 1439 829 631 605 122 107 71 BglII A'GATC_T Cuts at: 2206 5324 2206 Size: 3118 2211 BlpI GC'TnA_GC Cuts at: 2441 2746 2441 Size: 305 5024 Bme1580I G_kGCm'C Cuts at: 1790 3390 4546 1790 Size: 1600 1156 2573 Fragments arranged by size: 2573 1600 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5329 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2444 3060 4018 Size: 416 53 83 399 24 1027 616 958 Cuts at: 4018 442 Size: 1753 Fragments arranged by size: 1753 1027 958 616 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2105 1056 Size: 1049 4280 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 3989 1239 Size: 863 1887 2579 Fragments arranged by size: 2579 1887 863 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3454 4319 4617 3454 Size: 865 298 4166 Fragments arranged by size: 4166 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3992 882 Size: 617 2493 2219 Fragments arranged by size: 2493 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3120 493 Size: 711 1916 2702 Fragments arranged by size: 2702 1916 711 BsaBI GATnn'nnATC Cuts at: 5323 5323 Size: 5329 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3136 3518 4153 275 Size: 53 83 186 936 1603 382 635 1451 Fragments arranged by size: 1603 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2178 3762 4160 4434 4581 4992 2178 Size: 1584 398 274 147 411 2515 Fragments arranged by size: 2515 1584 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2560 2590 1178 Size: 30 165 30 1157 30 3917 Fragments arranged by size: 3917 1157 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2285 2586 1186 Size: 1099 301 3929 Fragments arranged by size: 3929 1099 301 BseYI C'CCAG_C Cuts at: 4532 5028 4532 Size: 496 4833 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4756 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2173 2779 3540 4568 1540 Size: 275 186 172 606 761 1028 2301 Fragments arranged by size: 2301 1028 761 606 275 186 172 BsiHKAI G_wGCw'C Cuts at: 2277 2385 3390 3475 4546 2277 Size: 108 1005 85 1071 3060 Fragments arranged by size: 3060 1071 1005 108 85 BsmI GAATG_Cn' Cuts at: 2369 2840 2933 2369 Size: 471 93 4765 Fragments arranged by size: 4765 471 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2277 2385 2392 2648 3390 3475 4546 Size: 399 487 108 7 256 742 85 1071 Cuts at: 4546 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 487 399 256 108 85 7 BspEI T'CCGG_A Cuts at: 4992 4992 Size: 5329 BspHI T'CATG_A Cuts at: 3218 4948 3218 Size: 1730 3599 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2684 844 Size: 1840 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2086 3216 1394 Size: 429 263 1130 3507 Fragments arranged by size: 3507 1130 429 263 BsrDI GCAATG_nn' Cuts at: 65 1248 3824 3998 65 Size: 1183 2576 174 1396 Fragments arranged by size: 2576 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3973 4160 1700 Size: 110 2163 187 2869 Fragments arranged by size: 2869 2163 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5329 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5329 BssSI C'ACGA_G Cuts at: 3389 4401 3389 Size: 1012 4317 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5329 BstBI TT'CG_AA Cuts at: 4133 4133 Size: 5329 BstEII G'GTnAC_C Cuts at: 1184 2414 1184 Size: 1230 4099 BstYI r'GATC_y Cuts at: 1972 2206 2659 3026 3411 3428 4880 4966 4978 Size: 234 453 367 385 17 1452 86 12 Cuts at: 4978 5324 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 453 385 367 346 234 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2142 2356 2419 2496 513 Size: 552 1077 214 63 77 3346 Fragments arranged by size: 3346 1077 552 214 77 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4590 Fragments arranged by size: 4590 683 56 BtsI GCAGTG_nn' Cuts at: 2845 3639 3659 2845 Size: 794 20 4515 Fragments arranged by size: 4515 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5329 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5294 DraI TTT'AAA Cuts at: 2633 2633 Size: 5329 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2183 4336 809 Size: 507 867 2153 1802 Fragments arranged by size: 2153 1802 867 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2170 2253 2493 Size: 54 1425 50 275 18 340 83 240 Cuts at: 2493 2776 3665 5294 8 Size: 283 889 1629 43 Fragments arranged by size: 1629 1425 889 340 283 275 240 83 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2170 2776 1537 Size: 275 358 606 4090 Fragments arranged by size: 4090 606 358 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2309 2589 3259 1353 Size: 956 280 670 3423 Fragments arranged by size: 3423 956 670 280 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3916 4290 4436 387 Size: 1064 138 2327 374 146 1280 Fragments arranged by size: 2327 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2383 2383 Size: 5329 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2102 3392 3989 4776 5044 1239 Size: 393 419 51 1290 597 787 268 1524 Fragments arranged by size: 1524 1290 787 597 419 393 268 51 EcoO109I rG'GnC_Cy Cuts at: 2320 2320 Size: 5329 EcoRI G'AATT_C Cuts at: 2638 3016 2638 Size: 378 4951 FspI TGC'GCA Cuts at: 3835 3835 Size: 5329 HaeII r_GCGC'y Cuts at: 1678 4476 1678 Size: 2798 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3970 4002 4044 4076 1373 Size: 32 135 32 2398 32 42 32 2626 Fragments arranged by size: 2626 2398 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2673 2919 669 Size: 988 281 735 246 3079 Fragments arranged by size: 3079 988 735 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5329 HpaI GTT'AAC Cuts at: 2919 2919 Size: 5329 KpnI G_GTAC'C Cuts at: 2563 2654 2563 Size: 91 5238 MfeI C'AATT_G Cuts at: 2928 2928 Size: 5329 MluI A'CGCG_T Cuts at: 5205 5205 Size: 5329 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4443 4627 1305 Size: 3138 184 2007 Fragments arranged by size: 3138 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2255 10 Size: 54 1425 343 423 3084 Fragments arranged by size: 3084 1425 423 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3287 3646 3805 518 Size: 648 2121 359 159 2042 Fragments arranged by size: 2121 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2152 2745 3422 4570 4815 1606 Size: 546 593 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 593 546 245 NcoI C'CATG_G Cuts at: 513 1065 2356 2419 513 Size: 552 1291 63 3423 Fragments arranged by size: 3423 1291 552 63 NdeI CA'TA_TG Cuts at: 387 387 Size: 5329 NheI G'CTAG_C Cuts at: 1052 2101 1052 Size: 1049 4280 NotI GC'GGCC_GC Cuts at: 2776 2776 Size: 5329 NspI r_CATG'y Cuts at: 1132 1229 2687 4232 1132 Size: 97 1458 1545 2229 Fragments arranged by size: 2229 1545 1458 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5329 PciI A'CATG_T Cuts at: 4228 4228 Size: 5329 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5329 PmeI GTTT'AAAC Cuts at: 2633 2633 Size: 5329 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3398 3430 4911 4943 3398 Size: 32 1481 32 3784 Fragments arranged by size: 3784 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2320 2320 Size: 5329 PshAI GACnn'nnGTC Cuts at: 1613 2325 1613 Size: 712 4617 PsiI TTA'TAA Cuts at: 2899 2899 Size: 5329 PstI C_TGCA'G Cuts at: 830 2050 2681 830 Size: 1220 631 3478 Fragments arranged by size: 3478 1220 631 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5329 PvuII CAG'CTG Cuts at: 2152 2152 Size: 5329 SacI G_AGCT'C Cuts at: 2385 2385 Size: 5329 SalI G'TCGA_C Cuts at: 1936 2671 1936 Size: 735 4594 SanDI GG'GwC_CC Cuts at: 2320 2320 Size: 5329 SbfI CC_TGCA'GG Cuts at: 2681 2681 Size: 5329 ScaI AGT'ACT Cuts at: 3577 5289 3577 Size: 1712 3617 SfcI C'TryA_G Cuts at: 826 1045 2046 2677 3812 4493 4684 826 Size: 219 1001 631 1135 681 191 1471 Fragments arranged by size: 1471 1135 1001 681 631 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5329 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5329 SmaI CCC'GGG Cuts at: 1872 2412 2656 1872 Size: 540 244 4545 Fragments arranged by size: 4545 540 244 SmlI C'TyrA_G Cuts at: 820 1017 1943 3433 4334 4596 820 Size: 197 926 1490 901 262 1553 Fragments arranged by size: 1553 1490 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3120 493 Size: 2627 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5329 SphI G_CATG'C Cuts at: 1132 2687 1132 Size: 1555 3774 SspI AAT'ATT Cuts at: 5 52 3253 5 Size: 47 3201 2081 Fragments arranged by size: 3201 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5329 StyI C'CwwG_G Cuts at: 513 1065 2161 2356 2419 2768 513 Size: 552 1096 195 63 349 3074 Fragments arranged by size: 3074 1096 552 349 195 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3356 3373 3526 3711 4156 325 Size: 1255 1776 17 153 185 445 1498 Fragments arranged by size: 1776 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3575 5287 96 Size: 274 80 33 51 1443 1598 1712 138 Fragments arranged by size: 1712 1598 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2173 2205 5289 5321 2173 Size: 32 3084 32 2181 Fragments arranged by size: 3084 2181 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5329 XbaI T'CTAG_A Cuts at: 2665 2665 Size: 5329 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2494 2494 Size: 5329 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5329 XmaI C'CCGG_G Cuts at: 1870 2410 2654 1870 Size: 540 244 4545 Fragments arranged by size: 4545 540 244 XmnI GAAnn'nnTTC Cuts at: 3458 3458 Size: 5329 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3137 276 Size: 53 83 186 936 1603 2468 Fragments arranged by size: 2468 1603 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI