(Linear) (Six-base) MAPSORT of: KIEE5270.seq Check: 1594 from: 1 to: 624 KIEE5270.orf With 182 enzymes: SgfI * December 5, 2014 13:00 .. Acc65I G'GTAC_C Cuts at: 0 554 624 Size: 554 70 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 624 Size: 46 578 AvaI C'yCGr_G Cuts at: 0 405 624 Size: 405 219 AvrII C'CTAG_G Cuts at: 0 156 624 Size: 156 468 BanI G'GyrC_C Cuts at: 0 554 624 Size: 554 70 BanII G_rGCy'C Cuts at: 0 380 387 624 Size: 380 7 237 Fragments arranged by size: 380 237 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 316 624 Size: 316 308 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 32 66 624 Size: 32 34 558 Fragments arranged by size: 558 34 32 BciVI GTATCCnnnnn_n' Cuts at: 0 144 624 Size: 144 480 BglI GCCn_nnn'nGGC Cuts at: 0 496 624 Size: 496 128 BglII A'GATC_T Cuts at: 0 201 624 Size: 201 423 BlpI GC'TnA_GC Cuts at: 0 436 624 Size: 436 188 BmrI ACTGGGnnnn_n' Cuts at: 0 439 624 Size: 439 185 BmtI G_CTAG'C Cuts at: 0 100 624 Size: 100 524 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 624 Size: 97 527 BsaWI w'CCGG_w Cuts at: 0 173 624 Size: 173 451 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 555 585 624 Size: 555 30 39 Fragments arranged by size: 555 39 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 280 581 624 Size: 280 301 43 Fragments arranged by size: 301 280 43 BsiEI CG_ry'CG Cuts at: 0 168 624 Size: 168 456 BsiHKAI G_wGCw'C Cuts at: 0 272 380 624 Size: 272 108 244 Fragments arranged by size: 272 244 108 BsmI GAATG_Cn' Cuts at: 0 364 624 Size: 364 260 Bsp1286I G_dGCh'C Cuts at: 0 272 380 387 624 Size: 272 108 7 237 Fragments arranged by size: 272 237 108 7 BsrBI CCG'CTC Cuts at: 0 81 624 Size: 81 543 BstEII G'GTnAC_C Cuts at: 0 409 624 Size: 409 215 BstYI r'GATC_y Cuts at: 0 201 624 Size: 201 423 BtgI C'CryG_G Cuts at: 0 137 351 414 491 624 Size: 137 214 63 77 133 Fragments arranged by size: 214 137 133 77 63 DrdI GACnn_nn'nnGTC Cuts at: 0 178 624 Size: 178 446 EaeI y'GGCC_r Cuts at: 0 165 248 488 624 Size: 165 83 240 136 Fragments arranged by size: 240 165 136 83 EagI C'GGCC_G Cuts at: 0 165 624 Size: 165 459 EarI CTCTTCn'nnn_ Cuts at: 0 304 584 624 Size: 304 280 40 Fragments arranged by size: 304 280 40 EcoICRI GAG'CTC Cuts at: 0 378 624 Size: 378 246 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 97 624 Size: 46 51 527 Fragments arranged by size: 527 51 46 EcoO109I rG'GnC_Cy Cuts at: 0 315 624 Size: 315 309 KpnI G_GTAC'C Cuts at: 0 558 624 Size: 558 66 MscI TGG'CCA Cuts at: 0 250 624 Size: 250 374 MspA1I CmG'CkG Cuts at: 0 147 624 Size: 147 477 NcoI C'CATG_G Cuts at: 0 351 414 624 Size: 351 63 210 Fragments arranged by size: 351 210 63 NheI G'CTAG_C Cuts at: 0 96 624 Size: 96 528 PpuMI rG'GwC_Cy Cuts at: 0 315 624 Size: 315 309 PshAI GACnn'nnGTC Cuts at: 0 320 624 Size: 320 304 PstI C_TGCA'G Cuts at: 0 45 624 Size: 45 579 PvuII CAG'CTG Cuts at: 0 147 624 Size: 147 477 SacI G_AGCT'C Cuts at: 0 380 624 Size: 380 244 SanDI GG'GwC_CC Cuts at: 0 315 624 Size: 315 309 SfcI C'TryA_G Cuts at: 0 41 624 Size: 41 583 SmaI CCC'GGG Cuts at: 0 407 624 Size: 407 217 StyI C'CwwG_G Cuts at: 0 156 351 414 624 Size: 156 195 63 210 Fragments arranged by size: 210 195 156 63 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 168 200 624 Size: 168 32 424 Fragments arranged by size: 424 168 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 489 624 Size: 489 135 XmaI C'CCGG_G Cuts at: 0 405 624 Size: 405 219 Enzymes that do cut: Acc65I AcuI AvaI AvrII BanI BanII BbsI BcgI BciVI BglI BglII BlpI BmrI BmtI BpmI BsaWI BsaXI BseRI BsiEI BsiHKAI BsmI Bsp1286I BsrBI BstEII BstYI BtgI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I KpnI MscI MspA1I NcoI NheI PpuMI PshAI PstI PvuII SacI SanDI SfcI SmaI StyI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI BaeI BamHI BbeI BbvCI BclI BfrBI Bme1580I BmgBI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BseYI BsgI BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI MfeI MluI MmeI MslI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI XmnI ZraI