(Circular) (Six-base) MAPSORT of: pFN21AE6821.seq Check: 778 from: 1 to: 5842 pFN21AE6821 5842 bp With 182 enzymes: SgfI * June 19, 2019 18:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3009 3009 Size: 5842 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3652 278 Size: 53 83 186 936 2116 2468 Fragments arranged by size: 2468 2116 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3185 1937 Size: 1248 4594 Acc65I G'GTAC_C Cuts at: 2139 2232 3163 2139 Size: 93 931 4818 Fragments arranged by size: 4818 931 93 AclI AA'CG_TT Cuts at: 1563 3969 4342 1563 Size: 2406 373 3063 Fragments arranged by size: 3063 2406 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3905 5289 5557 1632 Size: 2273 1384 268 1917 Fragments arranged by size: 2273 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5645 AflIII A'CryG_T Cuts at: 1203 4741 5718 1203 Size: 3538 977 1327 Fragments arranged by size: 3538 1327 977 AgeI A'CCGG_T Cuts at: 2690 4673 2690 Size: 1983 3859 AhdI GACnn_n'nnGTC Cuts at: 4571 4571 Size: 5842 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5842 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5842 AlwNI CAG_nnn'CTG Cuts at: 2247 2392 2596 3585 5157 2247 Size: 145 204 989 1572 2932 Fragments arranged by size: 2932 1572 989 204 145 ApaI G_GGCC'C Cuts at: 2059 2296 2059 Size: 237 5605 ApaLI G'TGCA_C Cuts at: 3899 5055 3899 Size: 1156 4686 ApoI r'AATT_y Cuts at: 1493 2458 3151 3377 3529 5638 1493 Size: 965 693 226 152 2109 1697 Fragments arranged by size: 2109 1697 965 693 226 152 AseI AT'TA_AT Cuts at: 160 1032 4396 160 Size: 872 3364 1606 Fragments arranged by size: 3364 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 AvaI C'yCGr_G Cuts at: 1870 1943 3167 1870 Size: 73 1224 4545 Fragments arranged by size: 4545 1224 73 BamHI G'GATC_C Cuts at: 3172 3539 3172 Size: 367 5475 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2139 2232 2626 2768 2997 Size: 325 217 627 352 93 394 142 229 Cuts at: 2997 3055 3163 4618 618 Size: 58 108 1455 1842 Fragments arranged by size: 1842 1455 627 394 352 325 229 217 142 108 93 58 BanII G_rGCy'C Cuts at: 1391 2059 2296 2529 3093 3161 1391 Size: 668 237 233 564 68 4072 Fragments arranged by size: 4072 668 564 237 233 68 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3050 928 Size: 971 1151 3720 Fragments arranged by size: 3720 1151 971 BbvCI CC'TCA_GC Cuts at: 2205 3049 2205 Size: 844 4998 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4033 4067 1903 Size: 34 50 34 2012 34 3678 Fragments arranged by size: 3678 2012 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3734 4944 3734 Size: 1210 4632 BclI T'GATC_A Cuts at: 1548 1548 Size: 5842 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2063 2415 4453 Size: 107 122 71 631 605 391 352 2038 Cuts at: 4453 136 Size: 1525 Fragments arranged by size: 2038 1525 631 605 391 352 122 107 71 BglII A'GATC_T Cuts at: 2433 5837 2433 Size: 3404 2438 BlpI GC'TnA_GC Cuts at: 3259 3259 Size: 5842 Bme1580I G_kGCm'C Cuts at: 1790 2059 2296 2771 3060 3903 5059 1790 Size: 269 237 475 289 843 1156 2573 Fragments arranged by size: 2573 1156 843 475 289 269 237 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5842 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3054 3573 4531 Size: 416 53 83 399 24 1637 519 958 Cuts at: 4531 442 Size: 1753 Fragments arranged by size: 1753 1637 958 519 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5842 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1999 2212 2269 2513 2768 2801 4502 1239 Size: 760 213 57 244 255 33 1701 2579 Fragments arranged by size: 2579 1701 760 255 244 213 57 33 Bpu10I CC'TnA_GC Cuts at: 2205 3049 3087 2205 Size: 844 38 4960 Fragments arranged by size: 4960 844 38 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2396 2879 2909 3967 4832 5130 2396 Size: 483 30 1058 865 298 3108 Fragments arranged by size: 3108 1058 865 483 298 30 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4505 882 Size: 617 3006 2219 Fragments arranged by size: 3006 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3633 493 Size: 711 2429 2702 Fragments arranged by size: 2702 2429 711 BsaBI GATnn'nnATC Cuts at: 5836 5836 Size: 5842 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3649 4031 4666 275 Size: 53 83 186 936 2116 382 635 1451 Fragments arranged by size: 2116 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2136 2690 4275 4673 4947 5094 5505 2136 Size: 554 1585 398 274 147 411 2473 Fragments arranged by size: 2473 1585 554 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2144 2174 2210 2240 1178 Size: 30 165 30 741 30 36 30 4780 Fragments arranged by size: 4780 741 165 36 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2222 2597 3083 1186 Size: 1036 375 486 3945 Fragments arranged by size: 3945 1036 486 375 BseYI C'CCAG_C Cuts at: 2170 2425 2682 5045 5541 2170 Size: 255 257 2363 496 2471 Fragments arranged by size: 2471 2363 496 257 255 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2601 1163 Size: 573 865 4404 Fragments arranged by size: 4404 865 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2343 3292 4053 5081 1540 Size: 275 186 342 949 761 1028 2301 Fragments arranged by size: 2301 1028 949 761 342 275 186 BsiHKAI G_wGCw'C Cuts at: 2130 2151 2529 3093 3903 3988 5059 2130 Size: 21 378 564 810 85 1071 2913 Fragments arranged by size: 2913 1071 810 564 378 85 21 BsmI GAATG_Cn' Cuts at: 3353 3446 3353 Size: 93 5749 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2059 2130 2151 2296 2529 2771 3060 Size: 399 269 71 21 145 233 242 289 Cuts at: 3060 3093 3161 3903 3988 5059 1391 Size: 33 68 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 289 269 242 233 145 85 71 68 33 21 BspEI T'CCGG_A Cuts at: 5505 5505 Size: 5842 BspHI T'CATG_A Cuts at: 3731 5461 3731 Size: 1730 4112 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2577 2962 3009 3197 844 Size: 1733 385 47 188 3489 Fragments arranged by size: 3489 1733 385 188 47 BsrBI CCG'CTC Cuts at: 1394 1823 3729 1394 Size: 429 1906 3507 Fragments arranged by size: 3507 1906 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2937 4337 4511 65 Size: 1183 1689 1400 174 1396 Fragments arranged by size: 1689 1400 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2690 4486 4673 1700 Size: 110 880 1796 187 2869 Fragments arranged by size: 2869 1796 880 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5842 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5842 BssSI C'ACGA_G Cuts at: 3902 4914 3902 Size: 1012 4830 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5842 BstBI TT'CG_AA Cuts at: 2542 4646 2542 Size: 2104 3738 BstEII G'GTnAC_C Cuts at: 1184 2095 2693 1184 Size: 911 598 4333 Fragments arranged by size: 4333 911 598 BstYI r'GATC_y Cuts at: 1972 2433 3172 3539 3924 3941 5393 5479 5491 Size: 461 739 367 385 17 1452 86 12 Cuts at: 5491 5837 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 739 461 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2287 2473 513 Size: 552 1222 186 3882 Fragments arranged by size: 3882 1222 552 186 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5103 Fragments arranged by size: 5103 683 56 BtsI GCAGTG_nn' Cuts at: 2448 2589 3358 4152 4172 2448 Size: 141 769 794 20 4118 Fragments arranged by size: 4118 794 769 141 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5842 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5807 DraI TTT'AAA Cuts at: 3146 3146 Size: 5842 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4849 809 Size: 507 3533 1802 Fragments arranged by size: 3533 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3289 4178 5807 Size: 54 1425 50 275 18 1459 889 1629 Cuts at: 5807 8 Size: 43 Fragments arranged by size: 1629 1459 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3289 1537 Size: 275 1477 4090 Fragments arranged by size: 4090 1477 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2879 3015 3772 1353 Size: 1526 136 757 3423 Fragments arranged by size: 3423 1526 757 136 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2273 4429 4803 4949 387 Size: 1064 138 684 2156 374 146 1280 Fragments arranged by size: 2156 1280 1064 684 374 146 138 EcoICRI GAG'CTC Cuts at: 2527 3091 2527 Size: 564 5278 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1999 2212 2269 2513 2768 2801 3905 Size: 393 367 213 57 244 255 33 1104 Cuts at: 3905 4502 5289 5557 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1104 787 597 393 367 268 255 244 213 57 33 EcoNI CCTnn'n_nnAGG Cuts at: 2204 2384 3005 2204 Size: 180 621 5041 Fragments arranged by size: 5041 621 180 EcoO109I rG'GnC_Cy Cuts at: 2055 2292 2293 2416 2055 Size: 237 1 123 5481 Fragments arranged by size: 5481 237 123 1 EcoRI G'AATT_C Cuts at: 3151 3529 3151 Size: 378 5464 EcoRV GAT'ATC Cuts at: 2504 2504 Size: 5842 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2086 2086 Size: 5842 FspI TGC'GCA Cuts at: 4348 4348 Size: 5842 HaeII r_GCGC'y Cuts at: 1678 4989 1678 Size: 3311 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4483 4515 4557 4589 1373 Size: 32 135 32 2911 32 42 32 2626 Fragments arranged by size: 2911 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3186 3432 669 Size: 988 281 1248 246 3079 Fragments arranged by size: 3079 1248 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5842 HpaI GTT'AAC Cuts at: 3432 3432 Size: 5842 KpnI G_GTAC'C Cuts at: 2143 2236 3167 2143 Size: 93 931 4818 Fragments arranged by size: 4818 931 93 MfeI C'AATT_G Cuts at: 3441 3441 Size: 5842 MluI A'CGCG_T Cuts at: 5718 5718 Size: 5842 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4956 5140 1305 Size: 3651 184 2007 Fragments arranged by size: 3651 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4020 Fragments arranged by size: 4020 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2360 3800 4159 4318 518 Size: 648 1194 1440 359 159 2042 Fragments arranged by size: 2042 1440 1194 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2170 2429 2593 2761 3053 3258 3935 5083 Size: 564 259 164 168 292 205 677 1148 Cuts at: 5083 5328 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 564 292 259 245 205 168 164 NcoI C'CATG_G Cuts at: 513 1065 2473 513 Size: 552 1408 3882 Fragments arranged by size: 3882 1408 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5842 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5842 NotI GC'GGCC_GC Cuts at: 3289 3289 Size: 5842 NspI r_CATG'y Cuts at: 1132 1229 3200 4745 1132 Size: 97 1971 1545 2229 Fragments arranged by size: 2229 1971 1545 97 PasI CC'CwG_GG Cuts at: 1348 2043 1348 Size: 695 5147 PciI A'CATG_T Cuts at: 4741 4741 Size: 5842 PflMI CCAn_nnn'nTGG Cuts at: 1108 2479 1108 Size: 1371 4471 PfoI T'CCnGG_A Cuts at: 2667 2778 2667 Size: 111 5731 PmeI GTTT'AAAC Cuts at: 3146 3146 Size: 5842 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3911 3943 5424 5456 3911 Size: 32 1481 32 4297 Fragments arranged by size: 4297 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5842 PsiI TTA'TAA Cuts at: 3412 3412 Size: 5842 PspOMI G'GGCC_C Cuts at: 2055 2292 2055 Size: 237 5605 PstI C_TGCA'G Cuts at: 830 2169 2198 2443 2959 3194 830 Size: 1339 29 245 516 235 3478 Fragments arranged by size: 3478 1339 516 245 235 29 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5842 PvuII CAG'CTG Cuts at: 2170 2429 2170 Size: 259 5583 SacI G_AGCT'C Cuts at: 2529 3093 2529 Size: 564 5278 SalI G'TCGA_C Cuts at: 1936 3184 1936 Size: 1248 4594 SbfI CC_TGCA'GG Cuts at: 3194 3194 Size: 5842 ScaI AGT'ACT Cuts at: 4090 5802 4090 Size: 1712 4130 SexAI A'CCwGG_T Cuts at: 2142 2142 Size: 5842 SfcI C'TryA_G Cuts at: 826 1045 2165 2194 2439 2955 3190 4325 5006 Size: 219 1120 29 245 516 235 1135 681 Cuts at: 5006 5197 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1120 681 516 245 235 219 191 29 SfiI GGCCn_nnn'nGGCC Cuts at: 2415 2415 Size: 5842 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5842 SmaI CCC'GGG Cuts at: 1872 3169 1872 Size: 1297 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2411 2858 2888 3946 4847 5109 Size: 197 926 468 447 30 1058 901 262 Cuts at: 5109 820 Size: 1553 Fragments arranged by size: 1553 1058 926 901 468 447 262 197 30 SnaBI TAC'GTA Cuts at: 493 3633 493 Size: 3140 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5842 SphI G_CATG'C Cuts at: 1132 3200 1132 Size: 2068 3774 SspI AAT'ATT Cuts at: 5 52 3766 5 Size: 47 3714 2081 Fragments arranged by size: 3714 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5842 StyI C'CwwG_G Cuts at: 513 1065 2070 2473 3281 513 Size: 552 1005 403 808 3074 Fragments arranged by size: 3074 1005 808 552 403 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3869 3886 4039 4224 4669 325 Size: 1255 2289 17 153 185 445 1498 Fragments arranged by size: 2289 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4088 5800 96 Size: 274 80 33 51 1443 2111 1712 138 Fragments arranged by size: 2111 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2144 2176 2210 2242 2382 2414 5802 5834 2144 Size: 32 34 32 140 32 3388 32 2152 Fragments arranged by size: 3388 2152 140 34 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5842 XbaI T'CTAG_A Cuts at: 3178 3178 Size: 5842 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5842 XmaI C'CCGG_G Cuts at: 1870 3167 1870 Size: 1297 4545 XmnI GAAnn'nnTTC Cuts at: 3971 3971 Size: 5842 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3650 276 Size: 53 83 186 936 2116 2468 Fragments arranged by size: 2468 2116 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SfoI SrfI SwaI XcmI