Restriction map for
pFN21AE3056
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# NO CUT ENZYMES ( 182 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AfaI AflII AflIII AgeI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsgI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmBI BsoBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BsrI BssHII
BssKI BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EagI EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
Ksp632I KspI MamI MluI MslI
MspA1I MvaI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI ScrFI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI TaqI TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 109 TCCGGA T^CCGGA
AciI 403 GCGG CCGC(-3/-1)
AcsI 483 AAATTT [GA]^AATT[CT]
AhdI 230 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 363 CCTAAGG CC^T[ACGT]AGG
ApoI 483 AAATTT [GA]^AATT[CT]
AspEI 230 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 47 TGGCCA TGG^CCA
BanII 445 GAGCCC G[GA]GC[CT]^C
BbvCI 144 CCTCAGC CCTCAGC(-5/-2)
BmyI 445 GAGCCC G[AGT]GC[ACT]^C
BsaWI 109 TCCGGA [AT]^CCGG[AT]
BseAI 109 TCCGGA T^CCGGA
BsmAI 319 GAGAC GTCTC(1/5)
BsmFI 291 GGGAC GGGAC(10/14)
BsmI 369 GAATGC GAATGC(1/-1)
Bsp1286I 445 GAGCCC G[AGT]GC[ACT]^C
BspEI 109 TCCGGA T^CCGGA
BsrDI 156 GCAATG GCAATG(2/0)
Bsu36I 363 CCTAAGG CC^T[ACGT]AGG
Cac8I 561 GCTAGC GC[ACGT]^[ACGT]GC
DraI 480 TTTAAA TTT^AAA
EaeI 47 TGGCCA [CT]^GGCC[GA]
Eam1105I 230 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 363 CCTAAGG CC^T[ACGT]AGG
EcoRV 6 GATATC GAT^ATC
HaeIII 48 GGCC GG^CC
HapII 110 CCGG C^CGG
HincII 70 GTTGAC GT[CT]^[GA]AC
HindII 70 GTTGAC GT[CT]^[GA]AC
HpaII 110 CCGG C^CGG
MaeII 417 ACGT A^CGT
MluNI 47 TGGCCA TGG^CCA
MroI 109 TCCGGA T^CCGGA
MscI 47 TGGCCA TGG^CCA
MspI 110 CCGG C^CGG
NheI 561 GCTAGC G^CTAGC
NlaIV 291 GGGACC GG[ACGT]^[ACGT]CC
PleI 515 GACTC GAGTC(4/5)
SwaI 479 ATTTAAAT ATTT^AAAT
TfiI 113 GATTC G^A[AT]TC
Tsp45I 608 GTCAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 107 GATCC GGATC(4/5)
547 GATCC
AvaII 229 GGACC G^G[AT]CC
292 GGACC
BfaI 254 CTAG C^TAG
562 CTAG
BseRI 79 GAGGAG GAGGAG(10/8)
82 GAGGAG
BstF5I 325 CATCC GGATG(2/0)
613 CATCC
BstYI 106 AGATCC [GA]^GATC[CT]
546 AGATCC
Cfr13I 229 GGACC G^G[ACGT]CC
292 GGACC
DpnII 107 GATC ^GATC
547 GATC
FokI 325 CATCC GGATG(9/13)
613 CATCC
HinfI 113 GATTC G^A[ACGT]TC
515 GACTC
HphI 116 TCACC GGTGA(8/7)
609 TCACC
MaeI 254 CTAG C^TAG
562 CTAG
MaeIII 164 GTAAC ^GT[ACGT]AC
608 GTCAC
MboI 107 GATC ^GATC
547 GATC
MboII 131 GAAGA GAAGA(8/7)
625 GAAGA
MfeI 455 CAATTG C^AATTG
617 CAATTG
MflI 106 AGATCC [GA]^GATC[CT]
546 AGATCC
MseI 208 TTAA T^TAA
481 TTAA
MunI 455 CAATTG C^AATTG
617 CAATTG
MwoI 498 GCCAACTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
525 GCTTTGTAAGC
NdeII 107 GATC ^GATC
547 GATC
Sau3AI 107 GATC ^GATC
547 GATC
Sau96I 229 GGACC G^G[ACGT]CC
292 GGACC
Tru9I 208 TTAA T^TAA
481 TTAA
XhoII 106 AGATCC [GA]^GATC[CT]
546 AGATCC
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
AluI BbvI DdeI Fnu4HI ItaI
MnlI NlaIII TseI Tsp509I