################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AvrII BalI BamHI BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI HgaI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspI SwaI Tru9I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcyI 872 GGCGCC G[GA]^CG[CT]C Alw44I 879 GTGCAC G^TGCAC ApaLI 879 GTGCAC G^TGCAC Asp700I 75 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 879 GTGCAC G[AT]GC[AT]^C AvaII 94 GGTCC G^G[AT]CC AviII 1006 TGCGCA TGC^GCA BanII 470 GAGCCC G[GA]GC[CT]^C BbeI 872 GGCGCC GGCGC^C BbvCI 712 GCTGAGG CCTCAGC(-5/-2) BcnI 603 CCGGG CC^[GC]GG BpmI 281 CTGGAG CTGGAG(16/14) BsaHI 872 GGCGCC G[GA]^CG[CT]C BseRI 154 GAGGAG GAGGAG(10/8) BsiHKAI 879 GTGCAC G[AT]GC[AT]^C BsmI 49 GCATTC GAATGC(1/-1) Bsp1407I 290 TGTACA T^GTACA BsrFI 499 GCCGGC [GA]^CCGG[CT] BsrGI 290 TGTACA T^GTACA BstAPI 49 GCATTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 325 TTCGAA TT^CGAA Cfr10I 499 GCCGGC [GA]^CCGG[CT] DraII 93 AGGTCCC [GA]G^G[ACGT]CC[CT] DsaI 372 CCATGG C^C[GA][CT]GG EcoNI 1035 CCTGGGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 93 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoRI 598 GAATTC G^AATTC FauI 709 CCCGC CCCGC(4/6) FspI 1006 TGCGCA TGC^GCA Hin1I 872 GGCGCC G[GA]^CG[CT]C KasI 872 GGCGCC G^GCGCC MaeII 861 ACGT A^CGT NaeI 499 GCCGGC GCC^GGC NarI 872 GGCGCC GG^CGCC NciI 603 CCGGG CC^[GC]GG NcoI 372 CCATGG C^CATGG NdeI 36 CATATG CA^TATG NgoMIV 499 GCCGGC G^CCGGC NspV 325 TTCGAA TT^CGAA PflMI 1029 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 284 GAGTC GAGTC(4/5) PpuMI 93 AGGTCCC [GA]G^G[AT]CC[CT] SapI 205 GCTCTTC GCTCTTC(1/4) SfoI 872 GGCGCC GGC^GCC SfuI 325 TTCGAA TT^CGAA SpeI 121 ACTAGT A^CTAGT SspBI 290 TGTACA T^GTACA Van91I 1029 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 428 CCAAACTCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 75 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwI 380 GGATC GGATC(4/5) 422 GGATC AlwNI 640 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG 1030 CAGTCCCTG BfaI 122 CTAG C^TAG 728 CTAG BsmAI 250 GAGAC GTCTC(1/5) 614 GAGAC BsmFI 95 GTCCC GGGAC(10/14) 1032 GTCCC BspMI 778 ACCTGC ACCTGC(4/8) 897 GCAGGT BstF5I 845 CATCC GGATG(2/0) 462 GGATG BstYI 380 GGATCT [GA]^GATC[CT] 422 GGATCT DraIII 700 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG 882 CACTGAGTG EarI 206 CTCTTC CTCTTC(1/4) 904 GAAGAG FokI 845 CATCC GGATG(9/13) 462 GGATG HapII 500 CCGG C^CGG 603 CCGG HpaII 500 CCGG C^CGG 603 CCGG Ksp632I 206 CTCTTC CTCTTC(1/4) 904 GAAGAG MaeI 122 CTAG C^TAG 728 CTAG MflI 380 GGATCT [GA]^GATC[CT] 422 GGATCT MslI 536 CATGCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 845 CATCCAGGTG MspI 500 CCGG C^CGG 603 CCGG StuI 101 AGGCCT AGG^CCT 716 AGGCCT TaqI 152 TCGA T^CGA 326 TCGA TfiI 480 GAATC G^A[AT]TC 760 GAATC Tsp45I 320 GTGAC ^GT[GC]AC 971 GTGAC TthHB8I 152 TCGA T^CGA 326 TCGA XhoII 380 GGATCT [GA]^GATC[CT] 422 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BanI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI HaeII HaeIII HhaI HinP1I HinfI HphI ItaI MaeIII MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp509I TspRI