(Circular) (Six-base) MAPSORT of: pFN21AE1833.seq Check: 6639 from: 1 to: 5758 pFN21AE1833 5758 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2893 2893 Size: 5758 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3568 278 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3101 1937 Size: 1164 4594 Acc65I G'GTAC_C Cuts at: 3079 3079 Size: 5758 AclI AA'CG_TT Cuts at: 1563 3885 4258 1563 Size: 2322 373 3063 Fragments arranged by size: 3063 2322 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2198 3821 5205 5473 1632 Size: 566 1623 1384 268 1917 Fragments arranged by size: 1917 1623 1384 566 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5561 AflIII A'CryG_T Cuts at: 1203 4657 5634 1203 Size: 3454 977 1327 Fragments arranged by size: 3454 1327 977 AgeI A'CCGG_T Cuts at: 4589 4589 Size: 5758 AhdI GACnn_n'nnGTC Cuts at: 4487 4487 Size: 5758 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5758 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5758 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2153 2185 2153 Size: 32 5726 AlwNI CAG_nnn'CTG Cuts at: 2650 3040 3501 5073 2650 Size: 390 461 1572 3335 Fragments arranged by size: 3335 1572 461 390 ApaLI G'TGCA_C Cuts at: 2884 3815 4971 2884 Size: 931 1156 3671 Fragments arranged by size: 3671 1156 931 ApoI r'AATT_y Cuts at: 1493 2414 2603 2948 3067 3293 3445 5554 1493 Size: 921 189 345 119 226 152 2109 1697 Fragments arranged by size: 2109 1697 921 345 226 189 152 119 AseI AT'TA_AT Cuts at: 160 1032 4312 160 Size: 872 3280 1606 Fragments arranged by size: 3280 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 AvaI C'yCGr_G Cuts at: 1870 1943 3083 1870 Size: 73 1140 4545 Fragments arranged by size: 4545 1140 73 BamHI G'GATC_C Cuts at: 3088 3455 3088 Size: 367 5391 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2141 2710 2877 3079 4534 Size: 325 217 627 354 569 167 202 1455 Cuts at: 4534 618 Size: 1842 Fragments arranged by size: 1842 1455 627 569 354 325 217 202 167 BanII G_rGCy'C Cuts at: 1391 2479 3077 1391 Size: 1088 598 4072 Fragments arranged by size: 4072 1088 598 BbeI G_GCGC'C Cuts at: 2881 2881 Size: 5758 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4787 BbvCI CC'TCA_GC Cuts at: 2718 2718 Size: 5758 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3949 3983 1903 Size: 34 2012 34 3678 Fragments arranged by size: 3678 2012 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3650 4860 3650 Size: 1210 4548 BclI T'GATC_A Cuts at: 1548 1548 Size: 5758 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4369 136 Size: 107 122 71 631 605 2697 1525 Fragments arranged by size: 2697 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5753 5753 Size: 5758 BlpI GC'TnA_GC Cuts at: 3175 3175 Size: 5758 Bme1580I G_kGCm'C Cuts at: 1790 2146 2715 2888 3034 3819 4975 1790 Size: 356 569 173 146 785 1156 2573 Fragments arranged by size: 2573 1156 785 569 356 173 146 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5758 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2716 3028 3489 Size: 416 53 83 399 24 1299 312 461 Cuts at: 3489 4447 442 Size: 958 1753 Fragments arranged by size: 1753 1299 958 461 416 399 312 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5758 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2307 4418 1239 Size: 1068 2111 2579 Fragments arranged by size: 2579 2111 1068 Bpu10I CC'TnA_GC Cuts at: 2718 2718 Size: 5758 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3883 4748 5046 3883 Size: 865 298 4595 Fragments arranged by size: 4595 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4421 882 Size: 617 2922 2219 Fragments arranged by size: 2922 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3549 493 Size: 711 2345 2702 Fragments arranged by size: 2702 2345 711 BsaBI GATnn'nnATC Cuts at: 5752 5752 Size: 5758 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2878 3565 3947 4582 Size: 53 83 186 936 1345 687 382 635 Cuts at: 4582 275 Size: 1451 Fragments arranged by size: 1451 1345 936 687 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4191 4589 4863 5010 5421 4191 Size: 398 274 147 411 4528 Fragments arranged by size: 4528 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2153 2183 1178 Size: 30 165 30 750 30 4753 Fragments arranged by size: 4753 750 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 1186 Size: 988 4770 BseYI C'CCAG_C Cuts at: 2684 4961 5457 2684 Size: 2277 496 2985 Fragments arranged by size: 2985 2277 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5185 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3208 3969 4997 1540 Size: 275 186 1207 761 1028 2301 Fragments arranged by size: 2301 1207 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2888 3819 3904 4975 2888 Size: 931 85 1071 3671 Fragments arranged by size: 3671 1071 931 85 BsmI GAATG_Cn' Cuts at: 2054 3269 3362 2054 Size: 1215 93 4450 Fragments arranged by size: 4450 1215 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2146 2479 2715 2888 3034 3077 3819 Size: 399 356 333 236 173 146 43 742 Cuts at: 3819 3904 4975 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 356 333 236 173 146 85 43 BspEI T'CCGG_A Cuts at: 5421 5421 Size: 5758 BspHI T'CATG_A Cuts at: 3647 5377 3647 Size: 1730 4028 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2792 2893 3113 844 Size: 1948 101 220 3489 Fragments arranged by size: 3489 1948 220 101 BsrBI CCG'CTC Cuts at: 1394 1823 3645 1394 Size: 429 1822 3507 Fragments arranged by size: 3507 1822 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4253 4427 65 Size: 1183 3005 174 1396 Fragments arranged by size: 3005 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2504 4402 4589 1700 Size: 110 694 1898 187 2869 Fragments arranged by size: 2869 1898 694 187 110 BsrGI T'GTAC_A Cuts at: 96 2295 96 Size: 2199 3559 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5758 BssSI C'ACGA_G Cuts at: 3818 4830 3818 Size: 1012 4746 BstAPI GCAn_nnn'nTGC Cuts at: 794 2060 794 Size: 1266 4492 BstBI TT'CG_AA Cuts at: 2331 4562 2331 Size: 2231 3527 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5758 BstYI r'GATC_y Cuts at: 1972 2385 2427 3088 3455 3840 3857 5309 5395 Size: 413 42 661 367 385 17 1452 86 Cuts at: 5395 5407 5753 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 661 413 385 367 346 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2377 513 Size: 552 939 373 3894 Fragments arranged by size: 3894 939 552 373 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5019 Fragments arranged by size: 5019 683 56 BtsI GCAGTG_nn' Cuts at: 2523 3034 3058 3274 4068 4088 2523 Size: 511 24 216 794 20 4193 Fragments arranged by size: 4193 794 511 216 24 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5758 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5723 DraI TTT'AAA Cuts at: 3062 3062 Size: 5758 DraIII CAC_nnn'GTG Cuts at: 2710 2892 2710 Size: 182 5576 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4765 809 Size: 507 3449 1802 Fragments arranged by size: 3449 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3205 4094 5723 Size: 54 1425 50 275 18 1375 889 1629 Cuts at: 5723 8 Size: 43 Fragments arranged by size: 1629 1425 1375 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3205 1537 Size: 275 1393 4090 Fragments arranged by size: 4090 1393 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2217 2904 3688 1353 Size: 864 687 784 3423 Fragments arranged by size: 3423 864 784 687 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2759 4345 4719 4865 387 Size: 1064 138 1170 1586 374 146 1280 Fragments arranged by size: 1586 1280 1170 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 2307 3821 4418 5205 5473 1239 Size: 393 566 109 1514 597 787 268 1524 Fragments arranged by size: 1524 1514 787 597 566 393 268 109 EcoNI CCTnn'n_nnAGG Cuts at: 3044 3044 Size: 5758 EcoO109I rG'GnC_Cy Cuts at: 2099 2099 Size: 5758 EcoRI G'AATT_C Cuts at: 2603 3067 3445 2603 Size: 464 378 4916 Fragments arranged by size: 4916 464 378 FspI TGC'GCA Cuts at: 3013 4264 3013 Size: 1251 4507 HaeII r_GCGC'y Cuts at: 1678 2651 2697 2881 4905 1678 Size: 973 46 184 2024 2531 Fragments arranged by size: 2531 2024 973 184 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2083 2115 4399 4431 4473 Size: 32 135 32 511 32 2284 32 42 Cuts at: 4473 4505 1373 Size: 32 2626 Fragments arranged by size: 2626 2284 511 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3102 3348 669 Size: 988 281 1164 246 3079 Fragments arranged by size: 3079 1164 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5758 HpaI GTT'AAC Cuts at: 3348 3348 Size: 5758 KasI G'GCGC_C Cuts at: 2877 2877 Size: 5758 KpnI G_GTAC'C Cuts at: 3083 3083 Size: 5758 MfeI C'AATT_G Cuts at: 3357 3357 Size: 5758 MluI A'CGCG_T Cuts at: 5634 5634 Size: 5758 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4872 5056 1305 Size: 3567 184 2007 Fragments arranged by size: 3567 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3936 Fragments arranged by size: 3936 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2545 2854 3716 4075 4234 518 Size: 648 1379 309 862 359 159 2042 Fragments arranged by size: 2042 1379 862 648 359 309 159 MspA1I CmG'CkG Cuts at: 1606 2596 2677 2717 2937 3174 3851 4999 5244 Size: 990 81 40 220 237 677 1148 245 Cuts at: 5244 1606 Size: 2120 Fragments arranged by size: 2120 1148 990 677 245 237 220 81 40 NaeI GCC'GGC Cuts at: 2506 2506 Size: 5758 NarI GG'CG_CC Cuts at: 2878 2878 Size: 5758 NcoI C'CATG_G Cuts at: 513 1065 2004 2377 513 Size: 552 939 373 3894 Fragments arranged by size: 3894 939 552 373 NdeI CA'TA_TG Cuts at: 387 2042 387 Size: 1655 4103 NgoMIV G'CCGG_C Cuts at: 2504 2504 Size: 5758 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5758 NotI GC'GGCC_GC Cuts at: 3205 3205 Size: 5758 NspI r_CATG'y Cuts at: 1132 1229 2273 2544 2586 2993 3116 4661 1132 Size: 97 1044 271 42 407 123 1545 2229 Fragments arranged by size: 2229 1545 1044 407 271 123 97 42 PasI CC'CwG_GG Cuts at: 1348 3040 1348 Size: 1692 4066 PciI A'CATG_T Cuts at: 4657 4657 Size: 5758 PflMI CCAn_nnn'nTGG Cuts at: 1108 3040 1108 Size: 1932 3826 PfoI T'CCnGG_A Cuts at: 2607 2607 Size: 5758 PmeI GTTT'AAAC Cuts at: 3062 3062 Size: 5758 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2153 2185 3827 3859 5340 5372 2153 Size: 32 1642 32 1481 32 2539 Fragments arranged by size: 2539 1642 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2099 2099 Size: 5758 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5758 PsiI TTA'TAA Cuts at: 3328 3328 Size: 5758 PstI C_TGCA'G Cuts at: 830 2402 2573 2595 2904 3110 830 Size: 1572 171 22 309 206 3478 Fragments arranged by size: 3478 1572 309 206 171 22 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5758 PvuII CAG'CTG Cuts at: 2596 2677 2937 2596 Size: 81 260 5417 Fragments arranged by size: 5417 260 81 SalI G'TCGA_C Cuts at: 1936 3100 1936 Size: 1164 4594 SapI GCTCTTCn'nnn_ Cuts at: 2217 2217 Size: 5758 SbfI CC_TGCA'GG Cuts at: 3110 3110 Size: 5758 ScaI AGT'ACT Cuts at: 4006 5718 4006 Size: 1712 4046 SfcI C'TryA_G Cuts at: 826 1045 2314 2398 2569 2591 2831 2900 3106 Size: 219 1269 84 171 22 240 69 206 Cuts at: 3106 4241 4922 5113 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1269 1135 681 240 219 206 191 171 84 69 22 SfoI GGC'GCC Cuts at: 2879 2879 Size: 5758 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5758 SmaI CCC'GGG Cuts at: 1872 3085 1872 Size: 1213 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3862 4763 5025 820 Size: 197 926 1919 901 262 1553 Fragments arranged by size: 1919 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3549 493 Size: 3056 2702 SpeI A'CTAG_T Cuts at: 152 2126 152 Size: 1974 3784 SphI G_CATG'C Cuts at: 1132 3116 1132 Size: 1984 3774 SspI AAT'ATT Cuts at: 5 52 3682 5 Size: 47 3630 2081 Fragments arranged by size: 3630 2081 47 StuI AGG'CCT Cuts at: 1517 2108 2723 1517 Size: 591 615 4552 Fragments arranged by size: 4552 615 591 StyI C'CwwG_G Cuts at: 513 1065 2004 2103 2136 2377 3197 513 Size: 552 939 99 33 241 820 3074 Fragments arranged by size: 3074 939 820 552 241 99 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2129 2240 2312 2698 3785 3802 3955 Size: 1255 549 111 72 386 1087 17 153 Cuts at: 3955 4140 4585 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1087 549 445 386 185 153 111 72 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2295 4004 5716 Size: 274 80 33 51 1443 318 1709 1712 Cuts at: 5716 96 Size: 138 Fragments arranged by size: 1712 1709 1443 318 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2834 2866 5718 5750 2834 Size: 32 2852 32 2842 Fragments arranged by size: 2852 2842 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5758 XbaI T'CTAG_A Cuts at: 3094 3094 Size: 5758 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2440 2440 Size: 5758 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5758 XmaI C'CCGG_G Cuts at: 1870 3083 1870 Size: 1213 4545 XmnI GAAnn'nnTTC Cuts at: 2084 3887 2084 Size: 1803 3955 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3566 276 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI