(Linear) (Six-base) MAPSORT of: KIEE1833.seq Check: 6487 from: 1 to: 1053 KIEE1833.orf With 182 enzymes: SgfI * July 15, 2010 18:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 888 1053 Size: 888 165 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 193 1053 Size: 193 860 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 148 180 1053 Size: 148 32 873 Fragments arranged by size: 873 148 32 AlwNI CAG_nnn'CTG Cuts at: 0 645 1035 1053 Size: 645 390 18 Fragments arranged by size: 645 390 18 ApaLI G'TGCA_C Cuts at: 0 879 1053 Size: 879 174 ApoI r'AATT_y Cuts at: 0 409 598 943 1053 Size: 409 189 345 110 Fragments arranged by size: 409 345 189 110 BanI G'GyrC_C Cuts at: 0 136 705 872 1053 Size: 136 569 167 181 Fragments arranged by size: 569 181 167 136 BanII G_rGCy'C Cuts at: 0 474 1053 Size: 474 579 BbeI G_GCGC'C Cuts at: 0 876 1053 Size: 876 177 BbvCI CC'TCA_GC Cuts at: 0 713 1053 Size: 713 340 Bme1580I G_kGCm'C Cuts at: 0 141 710 883 1029 1053 Size: 141 569 173 146 24 Fragments arranged by size: 569 173 146 141 24 BmrI ACTGGGnnnn_n' Cuts at: 0 711 1023 1053 Size: 711 312 30 Fragments arranged by size: 711 312 30 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 302 1053 Size: 302 751 Bpu10I CC'TnA_GC Cuts at: 0 713 1053 Size: 713 340 BsaHI Gr'CG_yC Cuts at: 0 873 1053 Size: 873 180 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 148 178 1053 Size: 148 30 875 Fragments arranged by size: 875 148 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 169 1053 Size: 169 884 BseYI C'CCAG_C Cuts at: 0 679 1053 Size: 679 374 BsiHKAI G_wGCw'C Cuts at: 0 883 1053 Size: 883 170 BsmI GAATG_Cn' Cuts at: 0 49 1053 Size: 49 1004 Bsp1286I G_dGCh'C Cuts at: 0 141 474 710 883 1029 1053 Size: 141 333 236 173 146 24 Fragments arranged by size: 333 236 173 146 141 24 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 787 888 1053 Size: 787 101 165 Fragments arranged by size: 787 165 101 BsrFI r'CCGG_y Cuts at: 0 499 1053 Size: 499 554 BsrGI T'GTAC_A Cuts at: 0 290 1053 Size: 290 763 BstAPI GCAn_nnn'nTGC Cuts at: 0 55 1053 Size: 55 998 BstBI TT'CG_AA Cuts at: 0 326 1053 Size: 326 727 BstYI r'GATC_y Cuts at: 0 380 422 1053 Size: 380 42 631 Fragments arranged by size: 631 380 42 BtgI C'CryG_G Cuts at: 0 372 1053 Size: 372 681 BtsI GCAGTG_nn' Cuts at: 0 518 1029 1053 Size: 518 511 24 Fragments arranged by size: 518 511 24 DraIII CAC_nnn'GTG Cuts at: 0 705 887 1053 Size: 705 182 166 Fragments arranged by size: 705 182 166 EarI CTCTTCn'nnn_ Cuts at: 0 212 899 1053 Size: 212 687 154 Fragments arranged by size: 687 212 154 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 754 1053 Size: 754 299 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 193 302 1053 Size: 193 109 751 Fragments arranged by size: 751 193 109 EcoNI CCTnn'n_nnAGG Cuts at: 0 1039 1053 Size: 1039 14 EcoO109I rG'GnC_Cy Cuts at: 0 94 1053 Size: 94 959 EcoRI G'AATT_C Cuts at: 0 598 1053 Size: 598 455 FspI TGC'GCA Cuts at: 0 1008 1053 Size: 1008 45 HaeII r_GCGC'y Cuts at: 0 646 692 876 1053 Size: 646 46 184 177 Fragments arranged by size: 646 184 177 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 78 110 1053 Size: 78 32 943 Fragments arranged by size: 943 78 32 KasI G'GCGC_C Cuts at: 0 872 1053 Size: 872 181 MslI CAynn'nnrTG Cuts at: 0 540 849 1053 Size: 540 309 204 Fragments arranged by size: 540 309 204 MspA1I CmG'CkG Cuts at: 0 591 672 712 932 1053 Size: 591 81 40 220 121 Fragments arranged by size: 591 220 121 81 40 NaeI GCC'GGC Cuts at: 0 501 1053 Size: 501 552 NarI GG'CG_CC Cuts at: 0 873 1053 Size: 873 180 NcoI C'CATG_G Cuts at: 0 372 1053 Size: 372 681 NdeI CA'TA_TG Cuts at: 0 37 1053 Size: 37 1016 NgoMIV G'CCGG_C Cuts at: 0 499 1053 Size: 499 554 NspI r_CATG'y Cuts at: 0 268 539 581 988 1053 Size: 268 271 42 407 65 Fragments arranged by size: 407 271 268 65 42 PasI CC'CwG_GG Cuts at: 0 1035 1053 Size: 1035 18 PflMI CCAn_nnn'nTGG Cuts at: 0 1035 1053 Size: 1035 18 PfoI T'CCnGG_A Cuts at: 0 602 1053 Size: 602 451 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 148 180 1053 Size: 148 32 873 Fragments arranged by size: 873 148 32 PpuMI rG'GwC_Cy Cuts at: 0 94 1053 Size: 94 959 PstI C_TGCA'G Cuts at: 0 397 568 590 899 1053 Size: 397 171 22 309 154 Fragments arranged by size: 397 309 171 154 22 PvuII CAG'CTG Cuts at: 0 591 672 932 1053 Size: 591 81 260 121 Fragments arranged by size: 591 260 121 81 SapI GCTCTTCn'nnn_ Cuts at: 0 212 1053 Size: 212 841 SfcI C'TryA_G Cuts at: 0 309 393 564 586 826 895 1053 Size: 309 84 171 22 240 69 158 Fragments arranged by size: 309 240 171 158 84 69 22 SfoI GGC'GCC Cuts at: 0 874 1053 Size: 874 179 SpeI A'CTAG_T Cuts at: 0 121 1053 Size: 121 932 StuI AGG'CCT Cuts at: 0 103 718 1053 Size: 103 615 335 Fragments arranged by size: 615 335 103 StyI C'CwwG_G Cuts at: 0 98 131 372 1053 Size: 98 33 241 681 Fragments arranged by size: 681 241 98 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 124 235 307 693 1053 Size: 124 111 72 386 360 Fragments arranged by size: 386 360 124 111 72 TatI w'GTAC_w Cuts at: 0 290 1053 Size: 290 763 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 829 861 1053 Size: 829 32 192 Fragments arranged by size: 829 192 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 435 1053 Size: 435 618 XmnI GAAnn'nnTTC Cuts at: 0 79 1053 Size: 79 974 Enzymes that do cut: AarI AcuI AloI AlwNI ApaLI ApoI BanI BanII BbeI BbvCI Bme1580I BmrI BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrFI BsrGI BstAPI BstBI BstYI BtgI BtsI DraIII EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I KasI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PflMI PfoI PpiI PpuMI PstI PvuII SapI SfcI SfoI SpeI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BssHII BssSI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EaeI EagI EcoICRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NheI NotI NruI NsiI PacI PciI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI ZraI