################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## Acc65I AccI AccII AclI AcsI AflII AgeI AhdI AluI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HgaI HhaI HinP1I HincII HindII HindIII HpaI ItaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MscI MspA1I MunI MvnI MwoI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI TseI Tth111I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 169 GACGTC GACGT^C AccIII 98 TCCGGA T^CCGGA AcyI 169 GACGTC G[GA]^CG[CT]C AflIII 103 ACGTGT A^C[GA][CT]GT Alw44I 409 GTGCAC G^TGCAC ApaLI 409 GTGCAC G^TGCAC AvaI 429 CTCGAG C^[CT]CG[GA]G AvaII 334 GGTCC G^G[AT]CC BbsI 84 GTCTTC GAAGAC(2/6) BpuAI 84 GTCTTC GAAGAC(2/6) BsaHI 169 GACGTC G[GA]^CG[CT]C BsaWI 98 TCCGGA [AT]^CCGG[AT] BseAI 98 TCCGGA T^CCGGA BsmI 252 GAATGC GAATGC(1/-1) BsoBI 429 CTCGAG C^[CT]CG[GA]G BspEI 98 TCCGGA T^CCGGA BstBI 272 TTCGAA TT^CGAA BstXI 114 CCATGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 76 GCTGGC GC[ACGT]^[ACGT]GC DraII 333 AGGTCCT [GA]G^G[ACGT]CC[CT] EarI 284 GAAGAG CTCTTC(1/4) EcoO109I 333 AGGTCCT [GA]G^G[ACGT]CC[CT] HapII 99 CCGG C^CGG Hin1I 169 GACGTC G[GA]^CG[CT]C HinfI 427 GACTC G^A[ACGT]TC HpaII 99 CCGG C^CGG HphI 156 TCACC GGTGA(8/7) Ksp632I 284 GAAGAG CTCTTC(1/4) MroI 98 TCCGGA T^CCGGA MspI 99 CCGG C^CGG NspI 405 GCATGT [GA]CATG^[CT] NspV 272 TTCGAA TT^CGAA PaeR7I 429 CTCGAG C^TCGAG PleI 427 GACTC GAGTC(4/5) PpuMI 333 AGGTCCT [GA]G^G[AT]CC[CT] ScaI 128 AGTACT AGT^ACT SfuI 272 TTCGAA TT^CGAA SmlI 429 CTCGAG C^T[CT][GA]AG StuI 151 AGGCCT AGG^CCT Tsp509I 321 AATT ^AATT XhoI 429 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AciI 44 CCGC CCGC(-3/-1) 111 CCGC AfaI 129 GTAC GT^AC 198 GTAC AlwI 96 GATCC GGATC(4/5) 187 GGATC AspHI 177 GTGCTC G[AT]GC[AT]^C 409 GTGCAC BmyI 177 GTGCTC G[AGT]GC[ACT]^C 409 GTGCAC BsiHKAI 177 GTGCTC G[AT]GC[AT]^C 409 GTGCAC Bsp1286I 177 GTGCTC G[AGT]GC[ACT]^C 409 GTGCAC BsrI 48 ACTGG ACTGG(1/-1) 211 ACTGG BstYI 95 AGATCC [GA]^GATC[CT] 187 GGATCT DpnII 96 GATC ^GATC 188 GATC DsaI 58 CCATGG C^C[GA][CT]GG 114 CCATGG EcoT14I 58 CCATGG C^C[AT][AT]GG 114 CCATGG MaeII 103 ACGT A^CGT 170 ACGT MboI 96 GATC ^GATC 188 GATC MboII 284 GAAGA GAAGA(8/7) 85 TCTTC MflI 95 AGATCC [GA]^GATC[CT] 187 GGATCT NcoI 58 CCATGG C^CATGG 114 CCATGG NdeII 96 GATC ^GATC 188 GATC PflMI 159 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 205 CCATTCACTGG RsaI 129 GTAC GT^AC 198 GTAC Sau3AI 96 GATC ^GATC 188 GATC StyI 58 CCATGG C^C[AT][AT]GG 114 CCATGG TaqI 273 TCGA T^CGA 430 TCGA TspRI 47 CACTG CA[GC]TG[ACGT][ACGT]^ 210 CACTG TthHB8I 273 TCGA T^CGA 430 TCGA Van91I 159 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 205 CCATTCACTGG XhoII 95 AGATCC [GA]^GATC[CT] 187 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### BsaJI BsiYI BslI BssKI BstNI Cfr13I EcoRII HaeIII MaeIII MnlI MseI MslI MvaI NlaIII Sau96I ScrFI Tru9I Tsp45I