(Linear) (Six-base) MAPSORT of: KIEE1635.seq  Check: 1173  from: 1  to: 453



KIEE1635.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AatII G_ACGT'C

Cuts at:      0     173     453 
   Size:        173     280
 
AflIII A'CryG_T

Cuts at:      0     103     453 
   Size:        103     350
 
AleI CACnn'nnGTG

Cuts at:      0     161     453 
   Size:        161     292
 
ApaLI G'TGCA_C

Cuts at:      0     409     453 
   Size:        409      44
 
AvaI C'yCGr_G

Cuts at:      0     429     453 
   Size:        429      24
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      77     453 
   Size:         77     376
 
Bme1580I G_kGCm'C

Cuts at:      0     413     453 
   Size:        413      40
 
BmgBI CAC'GTC

Cuts at:      0     104     453 
   Size:        104     349
 
BsaHI Gr'CG_yC

Cuts at:      0     170     453 
   Size:        170     283
 
BsaWI w'CCGG_w

Cuts at:      0      98     453 
   Size:         98     355
 
BsiHKAI G_wGCw'C

Cuts at:      0     181     413     453 
   Size:        181     232      40

  Fragments arranged by size: 

                 232     181      40
 
BsmI GAATG_Cn'

Cuts at:      0     258     453 
   Size:        258     195
 
Bsp1286I G_dGCh'C

Cuts at:      0     181     413     453 
   Size:        181     232      40

  Fragments arranged by size: 

                 232     181      40
 
BspEI T'CCGG_A

Cuts at:      0      98     453 
   Size:         98     355
 
BstBI TT'CG_AA

Cuts at:      0     273     453 
   Size:        273     180
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     121     453 
   Size:        121     332
 
BstYI r'GATC_y

Cuts at:      0      95     187     453 
   Size:         95      92     266

  Fragments arranged by size: 

                 266      95      92
 
BtgI C'CryG_G

Cuts at:      0      58     114     453 
   Size:         58      56     339

  Fragments arranged by size: 

                 339      58      56
 
EarI CTCTTCn'nnn_

Cuts at:      0     279     453 
   Size:        279     174
 
EcoO109I rG'GnC_Cy

Cuts at:      0     334     453 
   Size:        334     119
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      37     453 
   Size:         37     416
 
MslI CAynn'nnrTG

Cuts at:      0     119     161     214     453 
   Size:        119      42      53     239

  Fragments arranged by size: 

                 239     119      53      42
 
NcoI C'CATG_G

Cuts at:      0      58     114     453 
   Size:         58      56     339

  Fragments arranged by size: 

                 339      58      56
 
NspI r_CATG'y

Cuts at:      0     409     453 
   Size:        409      44
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     165     211     453 
   Size:        165      46     242

  Fragments arranged by size: 

                 242     165      46
 
PpuMI rG'GwC_Cy

Cuts at:      0     334     453 
   Size:        334     119
 
PspXI vC'TCGA_Gb

Cuts at:      0     429     453 
   Size:        429      24
 
ScaI AGT'ACT

Cuts at:      0     130     453 
   Size:        130     323
 
SmlI C'TyrA_G

Cuts at:      0     429     453 
   Size:        429      24
 
StuI AGG'CCT

Cuts at:      0     153     453 
   Size:        153     300
 
StyI C'CwwG_G

Cuts at:      0      58     114     453 
   Size:         58      56     339

  Fragments arranged by size: 

                 339      58      56
 
TatI w'GTAC_w

Cuts at:      0     128     453 
   Size:        128     325
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     160     179     192     206     211     238     453 
   Size:        160      19      13      14       5      27     215

  Fragments arranged by size: 

                 215     160      27      19      14      13       5
 
XhoI C'TCGA_G

Cuts at:      0     429     453 
   Size:        429      24
 
ZraI GAC'GTC

Cuts at:      0     171     453 
   Size:        171     282
 

 Enzymes that do cut:

    AatII   AflIII     AleI    ApaLI     AvaI     BbsI Bme1580I    BmgBI
    BsaHI    BsaWI  BsiHKAI     BsmI Bsp1286I    BspEI    BstBI    BstXI
    BstYI     BtgI     EarI EcoO109I     FalI     MslI     NcoI     NspI
    PflMI    PpuMI    PspXI     ScaI     SmlI     StuI     StyI     TatI
     TstI     XhoI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII  Tth111I     XbaI     XcmI     XmaI
     XmnI