################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BclI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspEI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstNI BstPI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRII EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HindIII HpaI KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MunI MvaI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 826 GTCTAC GT^[AC][GT]AC AccII 168 CGCG CG^CG AlwI 137 GGATC GGATC(4/5) AspI 424 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 157 CCCGGG C^[CT]CG[GA]G BbsI 808 GTCTTC GAAGAC(2/6) BbvCI 225 CCTCAGC CCTCAGC(-5/-2) BciVI 783 GGATAC GTATCC(6/5) BfaI 454 CTAG C^TAG BglII 816 AGATCT A^GATCT BpuAI 808 GTCTTC GAAGAC(2/6) BsaWI 134 ACCGGA [AT]^CCGG[AT] BsmAI 332 GAGAC GTCTC(1/5) BsoBI 157 CCCGGG C^[CT]CG[GA]G BspDI 773 ATCGAT AT^CGAT BsrBI 162 GAGCGG CCGCTC(-3/-3) BsrDI 484 GCAATG GCAATG(2/0) BstUI 168 CGCG CG^CG BstXI 291 CCAGCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 816 AGATCT [GA]^GATC[CT] Cac8I 52 GCGGGC GC[ACGT]^[ACGT]GC ClaI 773 ATCGAT AT^CGAT DsaI 447 CCATGG C^C[GA][CT]GG EarI 590 CTCTTC CTCTTC(1/4) EcoRI 752 GAATTC G^AATTC EcoT14I 447 CCATGG C^C[AT][AT]GG HincII 379 GTCAAC GT[CT]^[GA]AC HindII 379 GTCAAC GT[CT]^[GA]AC HphI 262 GGTGA GGTGA(8/7) Ksp632I 590 CTCTTC CTCTTC(1/4) MaeI 454 CTAG C^TAG MflI 816 AGATCT [GA]^GATC[CT] MseI 353 TTAA T^TAA MslI 359 CACCTGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 168 CGCG CG^CG NcoI 447 CCATGG C^CATGG PflFI 424 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 388 GAGCTC GAGCT^C SapI 589 GCTCTTC GCTCTTC(1/4) ScaI 723 AGTACT AGT^ACT SfaNI 128 GCATC GCATC(5/9) SfcI 789 CTATAG C^T[GA][CT]AG SmaI 157 CCCGGG CCC^GGG SmlI 385 CTTGAG C^T[CT][GA]AG StyI 447 CCATGG C^C[AT][AT]GG Tru9I 353 TTAA T^TAA TspRI 207 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 424 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 816 AGATCT [GA]^GATC[CT] XmaCI 157 CCCGGG C^CCGGG XmaI 157 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 550 GAATTT [GA]^AATT[CT] 752 GAATTC ApoI 550 GAATTT [GA]^AATT[CT] 752 GAATTC AspHI 388 GAGCTC G[AT]GC[AT]^C 587 GTGCTC BsgI 734 CTGCAC GTGCAG(16/14) 366 GTGCAG BsiHKAI 388 GAGCTC G[AT]GC[AT]^C 587 GTGCTC BspHI 110 TCATGA T^CATGA 852 TCATGA BsrI 327 ACTGG ACTGG(1/-1) 521 CCAGT BstBI 475 TTCGAA TT^CGAA 563 TTCGAA BstF5I 531 GGATG GGATG(2/0) 583 GGATG CfoI 126 GCGC GCG^C 167 GCGC EaeI 11 TGGCCG [CT]^GGCC[GA] 305 TGGCCG FokI 531 GGATG GGATG(9/13) 583 GGATG HgaI 78 GCGTC GACGC(5/10) 93 GCGTC HhaI 126 GCGC GCG^C 167 GCGC HinP1I 126 GCGC G^CGC 167 GCGC NspV 475 TTCGAA TT^CGAA 563 TTCGAA PvuII 502 CAGCTG CAG^CTG 731 CAGCTG RcaI 110 TCATGA T^CATGA 852 TCATGA SfuI 475 TTCGAA TT^CGAA 563 TTCGAA SspI 567 AATATT AAT^ATT 686 AATATT TfiI 265 GATTC G^A[AT]TC 473 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AfaI AluI AvaII BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI Cfr13I DdeI DpnII DraII EcoO109I FauI Fnu4HI HaeIII HapII HinfI HpaII ItaI MboI MboII MnlI MspA1I MspI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI TaqI TseI Tsp509I TthHB8I