(Circular) (Six-base) MAPSORT of: pFN21AE1789.seq Check: 1204 from: 1 to: 5593 pFN21AE1789 5593 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3403 278 Size: 53 83 186 936 1867 2468 Fragments arranged by size: 2468 1867 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2832 2936 1937 Size: 895 104 4594 Fragments arranged by size: 4594 895 104 Acc65I G'GTAC_C Cuts at: 2914 2914 Size: 5593 AclI AA'CG_TT Cuts at: 1563 3720 4093 1563 Size: 2157 373 3063 Fragments arranged by size: 3063 2157 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2904 3656 5040 5308 1632 Size: 1272 752 1384 268 1917 Fragments arranged by size: 1917 1384 1272 752 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5396 AflIII A'CryG_T Cuts at: 1203 4492 5469 1203 Size: 3289 977 1327 Fragments arranged by size: 3289 1327 977 AgeI A'CCGG_T Cuts at: 4424 4424 Size: 5593 AhdI GACnn_n'nnGTC Cuts at: 4322 4322 Size: 5593 AleI CACnn'nnGTG Cuts at: 1166 2368 1166 Size: 1202 4391 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5593 AlwNI CAG_nnn'CTG Cuts at: 3336 4908 3336 Size: 1572 4021 ApaLI G'TGCA_C Cuts at: 3650 4806 3650 Size: 1156 4437 ApoI r'AATT_y Cuts at: 1493 2555 2757 2902 3128 3280 5389 1493 Size: 1062 202 145 226 152 2109 1697 Fragments arranged by size: 2109 1697 1062 226 202 152 145 AseI AT'TA_AT Cuts at: 160 1032 4147 160 Size: 872 3115 1606 Fragments arranged by size: 3115 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5593 AvaI C'yCGr_G Cuts at: 1870 1943 2162 2918 1870 Size: 73 219 756 4545 Fragments arranged by size: 4545 756 219 73 BamHI G'GATC_C Cuts at: 2923 3290 2923 Size: 367 5226 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2914 4369 618 Size: 325 217 627 1127 1455 1842 Fragments arranged by size: 1842 1455 1127 627 325 217 BanII G_rGCy'C Cuts at: 1391 2063 2397 2882 2912 1391 Size: 672 334 485 30 4072 Fragments arranged by size: 4072 672 485 334 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2806 928 Size: 971 907 3715 Fragments arranged by size: 3715 971 907 BbvCI CC'TCA_GC Cuts at: 2231 2231 Size: 5593 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3784 3818 1903 Size: 34 1847 34 3678 Fragments arranged by size: 3678 1847 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2782 3485 4695 2782 Size: 703 1210 3680 Fragments arranged by size: 3680 1210 703 BclI T'GATC_A Cuts at: 1548 1548 Size: 5593 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4204 136 Size: 107 122 71 631 605 2532 1525 Fragments arranged by size: 2532 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2821 5588 2821 Size: 2767 2826 BlpI GC'TnA_GC Cuts at: 3010 3010 Size: 5593 Bme1580I G_kGCm'C Cuts at: 1790 3654 4810 1790 Size: 1864 1156 2573 Fragments arranged by size: 2573 1864 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5593 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2342 2520 3324 Size: 416 53 83 399 24 925 178 804 Cuts at: 3324 4282 442 Size: 958 1753 Fragments arranged by size: 1753 958 925 804 416 399 178 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5593 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4253 1239 Size: 3014 2579 Bpu10I CC'TnA_GC Cuts at: 2062 2231 2498 2062 Size: 169 267 5157 Fragments arranged by size: 5157 267 169 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2411 3718 4583 4881 2411 Size: 1307 865 298 3123 Fragments arranged by size: 3123 1307 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4256 882 Size: 617 2757 2219 Fragments arranged by size: 2757 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3384 493 Size: 711 2180 2702 Fragments arranged by size: 2702 2180 711 BsaBI GATnn'nnATC Cuts at: 5587 5587 Size: 5593 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3400 3782 4417 275 Size: 53 83 186 936 1867 382 635 1451 Fragments arranged by size: 1867 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2139 4026 4424 4698 4845 5256 2139 Size: 1887 398 274 147 411 2476 Fragments arranged by size: 2476 1887 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5368 Fragments arranged by size: 5368 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5593 BseYI C'CCAG_C Cuts at: 2053 2079 2263 4796 5292 2053 Size: 26 184 2533 496 2354 Fragments arranged by size: 2533 2354 496 184 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2392 2724 1163 Size: 573 656 332 4032 Fragments arranged by size: 4032 656 573 332 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3043 3804 4832 1540 Size: 275 186 1042 761 1028 2301 Fragments arranged by size: 2301 1042 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2397 2596 3654 3739 4810 2397 Size: 199 1058 85 1071 3180 Fragments arranged by size: 3180 1071 1058 199 85 BsmI GAATG_Cn' Cuts at: 3104 3197 3104 Size: 93 5500 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2397 2596 2882 2912 3654 3739 Size: 399 273 334 199 286 30 742 85 Cuts at: 3739 4810 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 334 286 273 199 85 30 BspEI T'CCGG_A Cuts at: 5256 5256 Size: 5593 BspHI T'CATG_A Cuts at: 2115 2857 3482 5212 2115 Size: 742 625 1730 2496 Fragments arranged by size: 2496 1730 742 625 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2948 844 Size: 2104 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2169 3480 1394 Size: 429 346 1311 3507 Fragments arranged by size: 3507 1311 429 346 BsrDI GCAATG_nn' Cuts at: 65 1248 2496 4088 4262 65 Size: 1183 1248 1592 174 1396 Fragments arranged by size: 1592 1396 1248 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4237 4424 1700 Size: 110 2427 187 2869 Fragments arranged by size: 2869 2427 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5593 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5593 BssSI C'ACGA_G Cuts at: 3653 4665 3653 Size: 1012 4581 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5593 BstBI TT'CG_AA Cuts at: 2481 2569 4397 2481 Size: 88 1828 3677 Fragments arranged by size: 3677 1828 88 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5593 BstXI CCAn_nnnn'nTGG Cuts at: 2303 2303 Size: 5593 BstYI r'GATC_y Cuts at: 1972 2821 2923 3290 3675 3692 5144 5230 5242 Size: 849 102 367 385 17 1452 86 12 Cuts at: 5242 5588 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 849 385 367 346 102 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2452 513 Size: 552 939 448 3654 Fragments arranged by size: 3654 939 552 448 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4854 Fragments arranged by size: 4854 683 56 BtsI GCAGTG_nn' Cuts at: 3109 3903 3923 3109 Size: 794 20 4779 Fragments arranged by size: 4779 794 20 ClaI AT'CG_AT Cuts at: 1554 2779 1554 Size: 1225 4368 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5558 DraI TTT'AAA Cuts at: 2897 2897 Size: 5593 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4600 809 Size: 507 3284 1802 Fragments arranged by size: 3284 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2310 3040 Size: 54 1425 50 275 18 186 294 730 Cuts at: 3040 3929 5558 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 730 294 275 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3040 1537 Size: 275 1228 4090 Fragments arranged by size: 4090 1228 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2601 3523 1353 Size: 1248 922 3423 Fragments arranged by size: 3423 1248 922 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2105 4180 4554 4700 387 Size: 1064 138 516 2075 374 146 1280 Fragments arranged by size: 2075 1280 1064 516 374 146 138 EcoICRI GAG'CTC Cuts at: 2395 2395 Size: 5593 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2904 3656 4253 5040 5308 1239 Size: 393 1272 752 597 787 268 1524 Fragments arranged by size: 1524 1272 787 752 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2050 2325 2416 2050 Size: 275 91 5227 Fragments arranged by size: 5227 275 91 EcoRI G'AATT_C Cuts at: 2757 2902 3280 2757 Size: 145 378 5070 Fragments arranged by size: 5070 378 145 FspI TGC'GCA Cuts at: 4099 4099 Size: 5593 HaeII r_GCGC'y Cuts at: 1678 4740 1678 Size: 3062 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4234 4266 4308 4340 1373 Size: 32 135 32 2662 32 42 32 2626 Fragments arranged by size: 2662 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2386 2937 3183 669 Size: 988 281 448 551 246 3079 Fragments arranged by size: 3079 988 551 448 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5593 HpaI GTT'AAC Cuts at: 3183 3183 Size: 5593 KpnI G_GTAC'C Cuts at: 2918 2918 Size: 5593 MfeI C'AATT_G Cuts at: 3192 3192 Size: 5593 MluI A'CGCG_T Cuts at: 5469 5469 Size: 5593 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2081 4707 4891 1305 Size: 776 2626 184 2007 Fragments arranged by size: 2626 2007 776 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3771 Fragments arranged by size: 3771 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2368 3551 3910 4069 518 Size: 648 1202 1183 359 159 2042 Fragments arranged by size: 2042 1202 1183 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2057 2404 2426 2509 2738 3009 3686 4834 Size: 451 347 22 83 229 271 677 1148 Cuts at: 4834 5079 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 451 347 271 245 229 83 22 NcoI C'CATG_G Cuts at: 513 1065 2004 2452 513 Size: 552 939 448 3654 Fragments arranged by size: 3654 939 552 448 NdeI CA'TA_TG Cuts at: 387 387 Size: 5593 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5593 NotI GC'GGCC_GC Cuts at: 3040 3040 Size: 5593 NspI r_CATG'y Cuts at: 1132 1229 2951 4496 1132 Size: 97 1722 1545 2229 Fragments arranged by size: 2229 1722 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5593 PciI A'CATG_T Cuts at: 4492 4492 Size: 5593 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5593 PmeI GTTT'AAAC Cuts at: 2897 2897 Size: 5593 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3662 3694 5175 5207 3662 Size: 32 1481 32 4048 Fragments arranged by size: 4048 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2050 2325 2416 2050 Size: 275 91 5227 Fragments arranged by size: 5227 275 91 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5593 PsiI TTA'TAA Cuts at: 3163 3163 Size: 5593 PstI C_TGCA'G Cuts at: 830 2945 830 Size: 2115 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5593 PvuII CAG'CTG Cuts at: 2509 2738 2509 Size: 229 5364 SacI G_AGCT'C Cuts at: 2397 2397 Size: 5593 SalI G'TCGA_C Cuts at: 1936 2935 1936 Size: 999 4594 SanDI GG'GwC_CC Cuts at: 2050 2325 2050 Size: 275 5318 SapI GCTCTTCn'nnn_ Cuts at: 2601 2601 Size: 5593 SbfI CC_TGCA'GG Cuts at: 2945 2945 Size: 5593 ScaI AGT'ACT Cuts at: 2730 3841 5553 2730 Size: 1111 1712 2770 Fragments arranged by size: 2770 1712 1111 SfcI C'TryA_G Cuts at: 826 1045 2794 2941 4076 4757 4948 826 Size: 219 1749 147 1135 681 191 1471 Fragments arranged by size: 1749 1471 1135 681 219 191 147 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5593 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5593 SmaI CCC'GGG Cuts at: 1872 2164 2920 1872 Size: 292 756 4545 Fragments arranged by size: 4545 756 292 SmlI C'TyrA_G Cuts at: 820 1017 1943 2390 3697 4598 4860 820 Size: 197 926 447 1307 901 262 1553 Fragments arranged by size: 1553 1307 926 901 447 262 197 SnaBI TAC'GTA Cuts at: 493 3384 493 Size: 2891 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5593 SphI G_CATG'C Cuts at: 1132 2951 1132 Size: 1819 3774 SspI AAT'ATT Cuts at: 5 52 2574 2693 3517 5 Size: 47 2522 119 824 2081 Fragments arranged by size: 2522 2081 824 119 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5593 StyI C'CwwG_G Cuts at: 513 1065 2004 2452 3032 513 Size: 552 939 448 580 3074 Fragments arranged by size: 3074 939 580 552 448 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2255 2422 3620 3637 3790 3975 4420 Size: 1255 675 167 1198 17 153 185 445 Cuts at: 4420 325 Size: 1498 Fragments arranged by size: 1498 1255 1198 675 445 185 167 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2279 2728 3839 Size: 274 80 33 51 1443 302 449 1111 Cuts at: 3839 5551 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1111 449 302 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5553 5585 5553 Size: 32 5561 Tth111I GACn'n_nGTC Cuts at: 1316 2432 1316 Size: 1116 4477 XbaI T'CTAG_A Cuts at: 2929 2929 Size: 5593 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5593 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5593 XmaI C'CCGG_G Cuts at: 1870 2162 2918 1870 Size: 292 756 4545 Fragments arranged by size: 4545 756 292 XmnI GAAnn'nnTTC Cuts at: 3722 3722 Size: 5593 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3401 276 Size: 53 83 186 936 1867 2468 Fragments arranged by size: 2468 1867 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI