(Circular) (Six-base) MAPSORT of: pFN21AB8472.seq Check: 6254 from: 1 to: 7225 pFN21AB8472 7225 bp With 182 enzymes: SgfI * June 19, 2019 18:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5035 278 Size: 53 83 186 936 3499 2468 Fragments arranged by size: 3499 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2607 4568 1937 Size: 670 1961 4594 Fragments arranged by size: 4594 1961 670 Acc65I G'GTAC_C Cuts at: 4546 4546 Size: 7225 AclI AA'CG_TT Cuts at: 1563 5352 5725 1563 Size: 3789 373 3063 Fragments arranged by size: 3789 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2399 2832 3255 3663 4101 5288 6672 6940 Size: 767 433 423 408 438 1187 1384 268 Cuts at: 6940 1632 Size: 1917 Fragments arranged by size: 1917 1384 1187 767 438 433 423 408 268 AflII C'TTAA_G Cuts at: 820 1017 2402 820 Size: 197 1385 5643 Fragments arranged by size: 5643 1385 197 AflIII A'CryG_T Cuts at: 1203 3592 6124 7101 1203 Size: 2389 2532 977 1327 Fragments arranged by size: 2532 2389 1327 977 AgeI A'CCGG_T Cuts at: 6056 6056 Size: 7225 AhdI GACnn_n'nnGTC Cuts at: 2773 5954 2773 Size: 3181 4044 AleI CACnn'nnGTG Cuts at: 1166 2736 4478 1166 Size: 1570 1742 3913 Fragments arranged by size: 3913 1742 1570 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2373 1184 Size: 1189 6036 AlwNI CAG_nnn'CTG Cuts at: 3374 3641 4968 6540 3374 Size: 267 1327 1572 4059 Fragments arranged by size: 4059 1572 1327 267 ApaLI G'TGCA_C Cuts at: 5282 6438 5282 Size: 1156 6069 ApoI r'AATT_y Cuts at: 1493 2335 2708 2744 2918 3071 3176 3223 4534 Size: 842 373 36 174 153 105 47 1311 Cuts at: 4534 4760 4912 7021 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1311 842 373 226 174 153 152 105 47 36 AseI AT'TA_AT Cuts at: 160 1032 2513 5779 160 Size: 872 1481 3266 1606 Fragments arranged by size: 3266 1606 1481 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7225 AvaI C'yCGr_G Cuts at: 1870 1943 3123 4550 1870 Size: 73 1180 1427 4545 Fragments arranged by size: 4545 1427 1180 73 BamHI G'GATC_C Cuts at: 3668 4282 4555 4922 3668 Size: 614 273 367 5971 Fragments arranged by size: 5971 614 367 273 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4546 6001 618 Size: 325 217 627 2759 1455 1842 Fragments arranged by size: 2759 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4544 1391 Size: 3153 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3728 4002 928 Size: 971 1829 274 4151 Fragments arranged by size: 4151 1829 971 274 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3058 3092 5416 5450 1903 Size: 34 1121 34 2324 34 3678 Fragments arranged by size: 3678 2324 1121 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5117 6327 5117 Size: 1210 6015 BclI T'GATC_A Cuts at: 1548 3816 4174 1548 Size: 2268 358 4599 Fragments arranged by size: 4599 2268 358 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3710 5836 136 Size: 107 122 71 631 605 2038 2126 1525 Fragments arranged by size: 2126 2038 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2851 7220 2851 Size: 4369 2856 BlpI GC'TnA_GC Cuts at: 4458 4642 4458 Size: 184 7041 Bme1580I G_kGCm'C Cuts at: 1790 5286 6442 1790 Size: 3496 1156 2573 Fragments arranged by size: 3496 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7225 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4956 5914 442 Size: 416 53 83 399 24 3539 958 1753 Fragments arranged by size: 3539 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7225 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3800 3800 Size: 7225 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2945 3213 3409 3854 5885 1239 Size: 1706 268 196 445 2031 2579 Fragments arranged by size: 2579 2031 1706 445 268 196 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2807 3350 3645 5350 6215 6513 2807 Size: 543 295 1705 865 298 3519 Fragments arranged by size: 3519 1705 865 543 298 295 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3647 5888 882 Size: 617 2148 2241 2219 Fragments arranged by size: 2241 2219 2148 617 BsaAI yAC'GTr Cuts at: 493 1204 5016 493 Size: 711 3812 2702 Fragments arranged by size: 3812 2702 711 BsaBI GATnn'nnATC Cuts at: 7219 7219 Size: 7225 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5032 5414 6049 275 Size: 53 83 186 936 3499 382 635 1451 Fragments arranged by size: 3499 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5658 6056 6330 6477 6888 5658 Size: 398 274 147 411 5995 Fragments arranged by size: 5995 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4154 4184 1178 Size: 30 165 30 2751 30 4219 Fragments arranged by size: 4219 2751 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3339 3399 3539 1186 Size: 2153 60 140 4872 Fragments arranged by size: 4872 2153 140 60 BseYI C'CCAG_C Cuts at: 6428 6924 6428 Size: 496 6729 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2089 2300 1163 Size: 573 353 211 6088 Fragments arranged by size: 6088 573 353 211 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2101 4675 5436 6464 1540 Size: 275 186 100 2574 761 1028 2301 Fragments arranged by size: 2574 2301 1028 761 275 186 100 BsiHKAI G_wGCw'C Cuts at: 5286 5371 6442 5286 Size: 85 1071 6069 Fragments arranged by size: 6069 1071 85 BsmI GAATG_Cn' Cuts at: 2225 3553 4736 4829 2225 Size: 1328 1183 93 4621 Fragments arranged by size: 4621 1328 1183 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4544 5286 5371 6442 1391 Size: 399 2754 742 85 1071 2174 Fragments arranged by size: 2754 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6888 6888 Size: 7225 BspHI T'CATG_A Cuts at: 5114 6844 5114 Size: 1730 5495 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3747 4580 844 Size: 2903 833 3489 Fragments arranged by size: 3489 2903 833 BsrBI CCG'CTC Cuts at: 1394 1823 3844 5112 1394 Size: 429 2021 1268 3507 Fragments arranged by size: 3507 2021 1268 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2941 3134 4233 5720 5894 65 Size: 1183 1693 193 1099 1487 174 1396 Fragments arranged by size: 1693 1487 1396 1183 1099 193 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2063 2110 5869 6056 1700 Size: 110 253 47 3759 187 2869 Fragments arranged by size: 3759 2869 253 187 110 47 BsrGI T'GTAC_A Cuts at: 96 3792 96 Size: 3696 3529 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7225 BssSI C'ACGA_G Cuts at: 5285 6297 5285 Size: 1012 6213 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7225 BstBI TT'CG_AA Cuts at: 6029 6029 Size: 7225 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7225 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3733 2011 Size: 1722 5503 BstYI r'GATC_y Cuts at: 1972 2563 2851 3370 3668 4119 4282 4555 4922 Size: 591 288 519 298 451 163 273 367 Cuts at: 4922 5307 5324 6776 6862 6874 7220 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 591 519 451 385 367 346 298 288 273 163 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3351 3351 Size: 7225 BtgI C'CryG_G Cuts at: 513 1065 2215 3463 4278 513 Size: 552 1150 1248 815 3460 Fragments arranged by size: 3460 1248 1150 815 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6486 Fragments arranged by size: 6486 683 56 BtsI GCAGTG_nn' Cuts at: 2526 2676 3021 3146 3870 4741 5535 5555 2526 Size: 150 345 125 724 871 794 20 4196 Fragments arranged by size: 4196 871 794 724 345 150 125 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7225 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2515 2550 2665 2700 3883 3918 529 Size: 35 1951 35 115 35 1183 35 3836 Fragments arranged by size: 3836 1951 1183 115 35 35 35 35 DraI TTT'AAA Cuts at: 2206 4529 2206 Size: 2323 4902 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3257 6232 809 Size: 507 1941 2975 1802 Fragments arranged by size: 2975 1941 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2218 2673 4672 Size: 54 1425 50 275 18 388 455 1999 Cuts at: 4672 5561 7190 8 Size: 889 1629 43 Fragments arranged by size: 1999 1629 1425 889 455 388 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4672 1537 Size: 275 2860 4090 Fragments arranged by size: 4090 2860 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2322 2574 3555 3594 5155 1353 Size: 969 252 981 39 1561 3423 Fragments arranged by size: 3423 1561 981 969 252 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5812 6186 6332 387 Size: 1064 138 4223 374 146 1280 Fragments arranged by size: 4223 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2399 2832 2945 3213 3255 3409 3663 Size: 393 767 433 113 268 42 154 254 Cuts at: 3663 3854 4101 5288 5885 6672 6940 1239 Size: 191 247 1187 597 787 268 1524 Fragments arranged by size: 1524 1187 787 767 597 433 393 268 268 254 247 191 154 113 42 EcoO109I rG'GnC_Cy Cuts at: 4445 4445 Size: 7225 EcoRI G'AATT_C Cuts at: 3223 4534 4912 3223 Size: 1311 378 5536 Fragments arranged by size: 5536 1311 378 FspI TGC'GCA Cuts at: 5731 5731 Size: 7225 HaeII r_GCGC'y Cuts at: 1678 6372 1678 Size: 4694 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2479 2511 2759 2791 3444 Size: 32 135 32 907 32 248 32 653 Cuts at: 3444 3476 4267 4299 5866 5898 5940 5972 1373 Size: 32 791 32 1567 32 42 32 2626 Fragments arranged by size: 2626 1567 907 791 653 248 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2419 4052 4569 4815 669 Size: 988 281 481 1633 517 246 3079 Fragments arranged by size: 3079 1633 988 517 481 281 246 HindIII A'AGCT_T Cuts at: 748 3896 4502 748 Size: 3148 606 3471 Fragments arranged by size: 3471 3148 606 HpaI GTT'AAC Cuts at: 4815 4815 Size: 7225 KpnI G_GTAC'C Cuts at: 4550 4550 Size: 7225 MfeI C'AATT_G Cuts at: 2145 4824 2145 Size: 2679 4546 MluI A'CGCG_T Cuts at: 7101 7101 Size: 7225 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4147 6339 6523 1305 Size: 2842 2192 184 2007 Fragments arranged by size: 2842 2192 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2675 10 Size: 54 1425 343 843 4560 Fragments arranged by size: 4560 1425 843 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2736 3139 4182 4478 5183 5542 Size: 648 843 727 403 1043 296 705 359 Cuts at: 5542 5701 518 Size: 159 2042 Fragments arranged by size: 2042 1043 843 727 705 648 403 359 296 159 MspA1I CmG'CkG Cuts at: 1606 2103 3233 4478 4641 5318 6466 6711 1606 Size: 497 1130 1245 163 677 1148 245 2120 Fragments arranged by size: 2120 1245 1148 1130 677 497 245 163 NaeI GCC'GGC Cuts at: 2065 2112 2065 Size: 47 7178 NcoI C'CATG_G Cuts at: 513 1065 2215 3463 4278 513 Size: 552 1150 1248 815 3460 Fragments arranged by size: 3460 1248 1150 815 552 NdeI CA'TA_TG Cuts at: 387 2269 2545 387 Size: 1882 276 5067 Fragments arranged by size: 5067 1882 276 NgoMIV G'CCGG_C Cuts at: 2063 2110 2063 Size: 47 7178 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7225 NotI GC'GGCC_GC Cuts at: 4672 4672 Size: 7225 NspI r_CATG'y Cuts at: 1132 1229 3596 4583 6128 1132 Size: 97 2367 987 1545 2229 Fragments arranged by size: 2367 2229 1545 987 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7225 PciI A'CATG_T Cuts at: 3592 6124 3592 Size: 2532 4693 PflMI CCAn_nnn'nTGG Cuts at: 1108 4424 1108 Size: 3316 3909 PfoI T'CCnGG_A Cuts at: 4071 4071 Size: 7225 PmeI GTTT'AAAC Cuts at: 4529 4529 Size: 7225 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5294 5326 6807 6839 5294 Size: 32 1481 32 5680 Fragments arranged by size: 5680 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7225 PsiI TTA'TAA Cuts at: 4795 4795 Size: 7225 PspXI vC'TCGA_Gb Cuts at: 3123 3123 Size: 7225 PstI C_TGCA'G Cuts at: 830 2533 4260 4577 830 Size: 1703 1727 317 3478 Fragments arranged by size: 3478 1727 1703 317 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7225 PvuII CAG'CTG Cuts at: 3233 4478 3233 Size: 1245 5980 SalI G'TCGA_C Cuts at: 1936 4567 1936 Size: 2631 4594 SapI GCTCTTCn'nnn_ Cuts at: 2322 3594 2322 Size: 1272 5953 SbfI CC_TGCA'GG Cuts at: 4577 4577 Size: 7225 ScaI AGT'ACT Cuts at: 3730 4336 5473 7185 3730 Size: 606 1137 1712 3770 Fragments arranged by size: 3770 1712 1137 606 SfcI C'TryA_G Cuts at: 826 1045 2289 2412 2529 3340 3378 4256 4573 Size: 219 1244 123 117 811 38 878 317 Cuts at: 4573 5708 6389 6580 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1244 1135 878 811 681 317 219 191 123 117 38 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7225 SgrAI Cr'CCGG_yG Cuts at: 1700 2063 1700 Size: 363 6862 SmaI CCC'GGG Cuts at: 1872 4552 1872 Size: 2680 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2402 2822 3123 3329 3660 5329 Size: 197 926 459 420 301 206 331 1669 Cuts at: 5329 6230 6492 820 Size: 901 262 1553 Fragments arranged by size: 1669 1553 926 901 459 420 331 301 262 206 197 SnaBI TAC'GTA Cuts at: 493 5016 493 Size: 4523 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7225 SphI G_CATG'C Cuts at: 1132 4583 1132 Size: 3451 3774 SspI AAT'ATT Cuts at: 5 52 5149 5 Size: 47 5097 2081 Fragments arranged by size: 5097 2081 47 StuI AGG'CCT Cuts at: 1517 3335 1517 Size: 1818 5407 StyI C'CwwG_G Cuts at: 513 1065 2197 2215 3463 3672 4278 4664 513 Size: 552 1132 18 1248 209 606 386 3074 Fragments arranged by size: 3074 1248 1132 606 552 386 209 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5252 5269 5422 5607 6052 325 Size: 1255 3672 17 153 185 445 1498 Fragments arranged by size: 3672 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3092 3728 3792 Size: 274 80 33 51 1443 1115 636 64 Cuts at: 3792 4334 5471 7183 96 Size: 542 1137 1712 138 Fragments arranged by size: 1712 1443 1137 1115 636 542 274 138 80 64 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7185 7217 7185 Size: 32 7193 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7225 XbaI T'CTAG_A Cuts at: 4561 4561 Size: 7225 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 4369 2011 Size: 2358 4867 XhoI C'TCGA_G Cuts at: 1943 3123 1943 Size: 1180 6045 XmaI C'CCGG_G Cuts at: 1870 4550 1870 Size: 2680 4545 XmnI GAAnn'nnTTC Cuts at: 2397 5354 2397 Size: 2957 4268 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5033 276 Size: 53 83 186 936 3499 2468 Fragments arranged by size: 3499 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI