Restriction map for
pFN21AE6644
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AlwNI
AocI Aor51HI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BbsI BbvCI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SspBI
SspI StuI SwaI TfiI Tru9I
TspRI Tth111I Van91I XbaI XcmI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflIII 275 ACGTGT A^C[GA][CT]GT
Alw44I 634 GTGCAC G^TGCAC
ApaLI 634 GTGCAC G^TGCAC
Asp700I 324 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 416 GGATCC G^GATCC
BanI 104 GGCGCC G^G[CT][GA]CC
BbeI 104 GGCGCC GGCGC^C
BbrPI 631 CACGTG CAC^GTG
BcgI 258 CGAGCTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 364 GAGACC GGTCTC(1/5)
BsmBI 50 CGTCTC CGTCTC(1/5)
BspMI 600 GCAGGT ACCTGC(4/8)
BsrFI 187 GCCGGC [GA]^CCGG[CT]
BstYI 416 GGATCC [GA]^GATC[CT]
Cfr10I 187 GCCGGC [GA]^CCGG[CT]
EaeI 146 TGGCCG [CT]^GGCC[GA]
EcoNI 8 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 104 GGCGCC [GA]GCGC^[CT]
HinfI 223 GACTC G^A[ACGT]TC
KasI 104 GGCGCC G^GCGCC
MboII 648 GAAGA GAAGA(8/7)
MflI 416 GGATCC [GA]^GATC[CT]
MspA1I 85 CCGCTG C[AC]G^C[GT]G
NaeI 187 GCCGGC GCC^GGC
NarI 104 GGCGCC GG^CGCC
NgoMIV 187 GCCGGC G^CCGGC
NspI 471 GCATGC [GA]CATG^[CT]
PaeR7I 256 CTCGAG C^TCGAG
PleI 223 GACTC GAGTC(4/5)
PmaCI 631 CACGTG CAC^GTG
PmlI 631 CACGTG CAC^GTG
PpuMI 167 GGGACCC [GA]G^G[AT]CC[CT]
SacI 259 GAGCTC GAGCT^C
SbfI 423 CCTGCAGG CCTGCA^GG
SfaNI 664 GATGC GCATC(5/9)
SfoI 104 GGCGCC GGC^GCC
SmlI 256 CTCGAG C^T[CT][GA]AG
SphI 471 GCATGC GCATG^C
Sse8387I 423 CCTGCAGG CCTGCA^GG
TaqI 257 TCGA T^CGA
Tsp509I 527 AATT ^AATT
TthHB8I 257 TCGA T^CGA
XhoI 256 CTCGAG C^TCGAG
XhoII 416 GGATCC [GA]^GATC[CT]
XmnI 324 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 78 CGCG CG^CG
375 CGCG
AcyI 48 GGCGTC G[GA]^CG[CT]C
104 GGCGCC
AfaI 279 GTAC GT^AC
681 GTAC
AluI 260 AGCT AG^CT
383 AGCT
AlwI 417 GATCC GGATC(4/5)
416 GGATC
AvaII 114 GGACC G^G[AT]CC
168 GGACC
BfaI 538 CTAG C^TAG
622 CTAG
BpmI 24 CTCCAG CTGGAG(16/14)
333 CTCCAG
BsaAI 274 TACGTG [CT]AC^GT[GA]
631 CACGTG
BsaHI 48 GGCGTC G[GA]^CG[CT]C
104 GGCGCC
BsmAI 364 GAGAC GTCTC(1/5)
51 GTCTC
BsmFI 167 GGGAC GGGAC(10/14)
354 GTCCC
BstEII 553 GGTGACC G^GT[ACGT]ACC
625 GGTGACC
BstF5I 309 GGATG GGATG(2/0)
663 GGATG
BstPI 553 GGTGACC G^GT[ACGT]ACC
625 GGTGACC
BstUI 78 CGCG CG^CG
375 CGCG
DdeI 543 CTCAG C^T[ACGT]AG
642 CTTAG
DsaI 287 CCATGG C^C[GA][CT]GG
476 CCATGG
EcoO65I 553 GGTGACC G^GT[ACGT]ACC
625 GGTGACC
FokI 309 GGATG GGATG(9/13)
663 GGATG
HgaI 49 GCGTC GACGC(5/10)
268 GCGTC
Hin1I 48 GGCGTC G[GA]^CG[CT]C
104 GGCGCC
MaeI 538 CTAG C^TAG
622 CTAG
MaeIII 554 GTGAC ^GT[ACGT]AC
626 GTGAC
MvnI 78 CGCG CG^CG
375 CGCG
NcoI 287 CCATGG C^CATGG
476 CCATGG
PstI 394 CTGCAG CTGCA^G
424 CTGCAG
RsaI 279 GTAC GT^AC
681 GTAC
SfcI 394 CTGCAG C^T[GA][CT]AG
424 CTGCAG
Tsp45I 554 GTGAC ^GT[GC]AC
626 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI ApaI AspHI AvaI BanII
BbvI BcnI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI BsoBI Bsp1286I
BsrI BssKI BstNI Cac8I CfoI
Cfr13I DpnII DraII EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeII MboI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SmaI StyI
TseI XmaCI XmaI