(Circular) (Six-base) MAPSORT of: pFN21AE6644.seq Check: 9343 from: 1 to: 5431 pFN21AE6644 5431 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2596 2596 Size: 5431 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3241 278 Size: 53 83 186 936 1705 2468 Fragments arranged by size: 2468 1705 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2774 1937 Size: 837 4594 Acc65I G'GTAC_C Cuts at: 2752 2752 Size: 5431 AclI AA'CG_TT Cuts at: 1563 2730 3558 3931 1563 Size: 1167 828 373 3063 Fragments arranged by size: 3063 1167 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2002 3494 4878 5146 1632 Size: 370 1492 1384 268 1917 Fragments arranged by size: 1917 1492 1384 370 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5234 AflIII A'CryG_T Cuts at: 1203 2280 4330 5307 1203 Size: 1077 2050 977 1327 Fragments arranged by size: 2050 1327 1077 977 AgeI A'CCGG_T Cuts at: 4262 4262 Size: 5431 AhdI GACnn_n'nnGTC Cuts at: 4160 4160 Size: 5431 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5431 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5431 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2321 2353 2321 Size: 32 5399 AlwNI CAG_nnn'CTG Cuts at: 3174 4746 3174 Size: 1572 3859 ApaI G_GGCC'C Cuts at: 2104 2187 2665 2104 Size: 83 478 4870 Fragments arranged by size: 4870 478 83 ApaLI G'TGCA_C Cuts at: 2639 3488 4644 2639 Size: 849 1156 3426 Fragments arranged by size: 3426 1156 849 ApoI r'AATT_y Cuts at: 1493 2740 2966 3118 5227 1493 Size: 1247 226 152 2109 1697 Fragments arranged by size: 2109 1697 1247 226 152 AseI AT'TA_AT Cuts at: 160 1032 3985 160 Size: 872 2953 1606 Fragments arranged by size: 2953 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 AvaI C'yCGr_G Cuts at: 1870 1943 2104 2169 2202 2261 2310 2361 2756 Size: 73 161 65 33 59 49 51 395 Cuts at: 2756 1870 Size: 4545 Fragments arranged by size: 4545 395 161 73 65 59 51 49 33 BamHI G'GATC_C Cuts at: 2421 2761 3128 2421 Size: 340 367 4724 Fragments arranged by size: 4724 367 340 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2109 2752 4207 618 Size: 325 217 627 322 643 1455 1842 Fragments arranged by size: 1842 1455 643 627 325 322 217 BanII G_rGCy'C Cuts at: 1391 2104 2187 2268 2665 2750 1391 Size: 713 83 81 397 85 4072 Fragments arranged by size: 4072 713 397 85 83 81 BbeI G_GCGC'C Cuts at: 2113 2113 Size: 5431 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4460 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2252 2286 3622 3656 1903 Size: 34 50 34 231 34 1336 34 3678 Fragments arranged by size: 3678 1336 231 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3323 4533 3323 Size: 1210 4221 BclI T'GATC_A Cuts at: 1548 1548 Size: 5431 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4042 136 Size: 107 122 71 631 605 2370 1525 Fragments arranged by size: 2370 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5426 5426 Size: 5431 BlpI GC'TnA_GC Cuts at: 2848 2848 Size: 5431 Bme1580I G_kGCm'C Cuts at: 1790 2104 2187 2643 2665 3492 4648 1790 Size: 314 83 456 22 827 1156 2573 Fragments arranged by size: 2573 1156 827 456 314 83 22 BmgBI CAC'GTC Cuts at: 1318 2320 1318 Size: 1002 4429 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2470 3162 4120 Size: 416 53 83 399 24 1053 692 958 Cuts at: 4120 442 Size: 1753 Fragments arranged by size: 1753 1053 958 692 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5431 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2014 2323 4091 1239 Size: 775 309 1768 2579 Fragments arranged by size: 2579 1768 775 309 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3556 4421 4719 3556 Size: 865 298 4268 Fragments arranged by size: 4268 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2363 4094 882 Size: 617 864 1731 2219 Fragments arranged by size: 2219 1731 864 617 BsaAI yAC'GTr Cuts at: 493 1204 2281 2638 3222 493 Size: 711 1077 357 584 2702 Fragments arranged by size: 2702 1077 711 584 357 BsaBI GATnn'nnATC Cuts at: 5425 5425 Size: 5431 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2054 2110 3238 3620 Size: 53 83 186 936 521 56 1128 382 Cuts at: 3620 4255 275 Size: 635 1451 Fragments arranged by size: 1451 1128 936 635 521 382 186 83 56 53 BsaWI w'CCGG_w Cuts at: 3864 4262 4536 4683 5094 3864 Size: 398 274 147 411 4201 Fragments arranged by size: 4201 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2213 2243 2321 2351 1178 Size: 30 165 30 810 30 78 30 4258 Fragments arranged by size: 4258 810 165 78 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2015 2155 2381 2427 1186 Size: 829 140 226 46 4190 Fragments arranged by size: 4190 829 226 140 46 BseYI C'CCAG_C Cuts at: 4634 5130 4634 Size: 496 4935 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4858 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2881 3642 4670 1540 Size: 275 186 880 761 1028 2301 Fragments arranged by size: 2301 1028 880 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2268 2379 2494 2643 3492 3577 4648 2268 Size: 111 115 149 849 85 1071 3051 Fragments arranged by size: 3051 1071 849 149 115 111 85 BsmI GAATG_Cn' Cuts at: 2942 3035 2942 Size: 93 5338 BsmBI CGTCTCn'nnnn_ Cuts at: 2061 2061 Size: 5431 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2104 2187 2268 2379 2494 2643 2665 Size: 399 314 83 81 111 115 149 22 Cuts at: 2665 2750 3492 3577 4648 1391 Size: 85 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 314 149 115 111 85 85 83 81 22 BspEI T'CCGG_A Cuts at: 5094 5094 Size: 5431 BspHI T'CATG_A Cuts at: 3320 5050 3320 Size: 1730 3701 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2596 2786 844 Size: 1752 190 3489 Fragments arranged by size: 3489 1752 190 BsrBI CCG'CTC Cuts at: 1394 1823 3318 1394 Size: 429 1495 3507 Fragments arranged by size: 3507 1495 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3926 4100 65 Size: 1183 2678 174 1396 Fragments arranged by size: 2678 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2192 4075 4262 1700 Size: 110 382 1883 187 2869 Fragments arranged by size: 2869 1883 382 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5431 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5431 BssSI C'ACGA_G Cuts at: 3491 4503 3491 Size: 1012 4419 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5431 BstBI TT'CG_AA Cuts at: 4235 4235 Size: 5431 BstEII G'GTnAC_C Cuts at: 1184 2558 2630 1184 Size: 1374 72 3985 Fragments arranged by size: 3985 1374 72 BstYI r'GATC_y Cuts at: 1972 2421 2761 3128 3513 3530 4982 5068 5080 Size: 449 340 367 385 17 1452 86 12 Cuts at: 5080 5426 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 449 385 367 346 340 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2292 2481 513 Size: 552 1227 189 3463 Fragments arranged by size: 3463 1227 552 189 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4692 Fragments arranged by size: 4692 683 56 BtsI GCAGTG_nn' Cuts at: 2947 3741 3761 2947 Size: 794 20 4617 Fragments arranged by size: 4617 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5431 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5396 DraI TTT'AAA Cuts at: 2735 2735 Size: 5431 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4438 809 Size: 507 3122 1802 Fragments arranged by size: 3122 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2151 2878 3767 Size: 54 1425 50 275 18 321 727 889 Cuts at: 3767 5396 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 727 321 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2878 1537 Size: 275 1066 4090 Fragments arranged by size: 4090 1066 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3361 1353 Size: 2008 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2414 2696 4018 4392 4538 387 Size: 1064 138 825 282 1322 374 146 1280 Fragments arranged by size: 1322 1280 1064 825 374 282 146 138 EcoICRI GAG'CTC Cuts at: 2266 2266 Size: 5431 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2002 2014 2323 3494 4091 4878 5146 Size: 393 370 12 309 1171 597 787 268 Cuts at: 5146 1239 Size: 1524 Fragments arranged by size: 1524 1171 787 597 393 370 309 268 12 EcoNI CCTnn'n_nnAGG Cuts at: 2017 2017 Size: 5431 EcoO109I rG'GnC_Cy Cuts at: 2101 2173 2661 2662 2101 Size: 72 488 1 4870 Fragments arranged by size: 4870 488 72 1 EcoRI G'AATT_C Cuts at: 2740 3118 2740 Size: 378 5053 FspI TGC'GCA Cuts at: 3937 3937 Size: 5431 HaeII r_GCGC'y Cuts at: 1678 2113 4578 1678 Size: 435 2465 2531 Fragments arranged by size: 2531 2465 435 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2406 2438 4072 4104 4146 Size: 32 135 32 834 32 1634 32 42 Cuts at: 4146 4178 1373 Size: 32 2626 Fragments arranged by size: 2626 1634 834 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2775 3021 669 Size: 988 281 837 246 3079 Fragments arranged by size: 3079 988 837 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5431 HpaI GTT'AAC Cuts at: 3021 3021 Size: 5431 KasI G'GCGC_C Cuts at: 2109 2109 Size: 5431 KpnI G_GTAC'C Cuts at: 2756 2756 Size: 5431 MfeI C'AATT_G Cuts at: 3030 3030 Size: 5431 MluI A'CGCG_T Cuts at: 5307 5307 Size: 5431 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4545 4729 1305 Size: 3240 184 2007 Fragments arranged by size: 3240 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3609 Fragments arranged by size: 3609 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3389 3748 3907 518 Size: 648 2223 359 159 2042 Fragments arranged by size: 2223 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2092 2847 3524 4672 4917 1606 Size: 486 755 677 1148 245 2120 Fragments arranged by size: 2120 1148 755 677 486 245 NaeI GCC'GGC Cuts at: 2194 2194 Size: 5431 NarI GG'CG_CC Cuts at: 2110 2110 Size: 5431 NcoI C'CATG_G Cuts at: 513 1065 2292 2481 513 Size: 552 1227 189 3463 Fragments arranged by size: 3463 1227 552 189 NdeI CA'TA_TG Cuts at: 387 387 Size: 5431 NgoMIV G'CCGG_C Cuts at: 2192 2192 Size: 5431 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5431 NotI GC'GGCC_GC Cuts at: 2878 2878 Size: 5431 NspI r_CATG'y Cuts at: 1132 1229 2480 2789 4334 1132 Size: 97 1251 309 1545 2229 Fragments arranged by size: 2229 1545 1251 309 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5431 PciI A'CATG_T Cuts at: 4330 4330 Size: 5431 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5431 PfoI T'CCnGG_A Cuts at: 2580 2580 Size: 5431 PmeI GTTT'AAAC Cuts at: 2735 2735 Size: 5431 PmlI CAC'GTG Cuts at: 2638 2638 Size: 5431 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2321 2353 3500 3532 5013 5045 2321 Size: 32 1147 32 1481 32 2707 Fragments arranged by size: 2707 1481 1147 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2173 2173 Size: 5431 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5431 PsiI TTA'TAA Cuts at: 3001 3001 Size: 5431 PspOMI G'GGCC_C Cuts at: 2100 2183 2661 2100 Size: 83 478 4870 Fragments arranged by size: 4870 478 83 PspXI vC'TCGA_Gb Cuts at: 2261 2261 Size: 5431 PstI C_TGCA'G Cuts at: 830 2403 2433 2783 830 Size: 1573 30 350 3478 Fragments arranged by size: 3478 1573 350 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5431 SacI G_AGCT'C Cuts at: 2268 2268 Size: 5431 SalI G'TCGA_C Cuts at: 1936 2773 1936 Size: 837 4594 SanDI GG'GwC_CC Cuts at: 2173 2173 Size: 5431 SbfI CC_TGCA'GG Cuts at: 2433 2783 2433 Size: 350 5081 ScaI AGT'ACT Cuts at: 3679 5391 3679 Size: 1712 3719 SfcI C'TryA_G Cuts at: 826 1045 2399 2429 2779 3914 4595 4786 826 Size: 219 1354 30 350 1135 681 191 1471 Fragments arranged by size: 1471 1354 1135 681 350 219 191 30 SfoI GGC'GCC Cuts at: 2111 2111 Size: 5431 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5431 SmaI CCC'GGG Cuts at: 1872 2106 2171 2312 2363 2758 1872 Size: 234 65 141 51 395 4545 Fragments arranged by size: 4545 395 234 141 65 51 SmlI C'TyrA_G Cuts at: 820 1017 1943 2261 3535 4436 4698 820 Size: 197 926 318 1274 901 262 1553 Fragments arranged by size: 1553 1274 926 901 318 262 197 SnaBI TAC'GTA Cuts at: 493 3222 493 Size: 2729 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5431 SphI G_CATG'C Cuts at: 1132 2480 2789 1132 Size: 1348 309 3774 Fragments arranged by size: 3774 1348 309 SspI AAT'ATT Cuts at: 5 52 3355 5 Size: 47 3303 2081 Fragments arranged by size: 3303 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5431 StyI C'CwwG_G Cuts at: 513 1065 2115 2292 2481 2707 2870 513 Size: 552 1050 177 189 226 163 3074 Fragments arranged by size: 3074 1050 552 226 189 177 163 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2387 3458 3475 3628 3813 4258 325 Size: 1255 807 1071 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1071 807 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2283 3677 5389 Size: 274 80 33 51 1443 306 1394 1712 Cuts at: 5389 96 Size: 138 Fragments arranged by size: 1712 1443 1394 306 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2213 2245 5391 5423 2213 Size: 32 3146 32 2221 Fragments arranged by size: 3146 2221 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5431 XbaI T'CTAG_A Cuts at: 2767 2767 Size: 5431 XhoI C'TCGA_G Cuts at: 1943 2261 1943 Size: 318 5113 XmaI C'CCGG_G Cuts at: 1870 2104 2169 2310 2361 2756 1870 Size: 234 65 141 51 395 4545 Fragments arranged by size: 4545 395 234 141 65 51 XmnI GAAnn'nnTTC Cuts at: 2333 3560 2333 Size: 1227 4204 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3239 276 Size: 53 83 186 936 1705 2468 Fragments arranged by size: 2468 1705 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PsrI PvuII RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI