(Linear) (Six-base) MAPSORT of: KIEE6644.seq Check: 222 from: 1 to: 726 KIEE6644.orf With 182 enzymes: SgfI * March 16, 2015 11:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 591 726 Size: 591 135 AflIII A'CryG_T Cuts at: 0 275 726 Size: 275 451 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 316 348 726 Size: 316 32 378 Fragments arranged by size: 378 316 32 ApaI G_GGCC'C Cuts at: 0 99 182 660 726 Size: 99 83 478 66 Fragments arranged by size: 478 99 83 66 ApaLI G'TGCA_C Cuts at: 0 634 726 Size: 634 92 AvaI C'yCGr_G Cuts at: 0 99 164 197 256 305 356 726 Size: 99 65 33 59 49 51 370 Fragments arranged by size: 370 99 65 59 51 49 33 BamHI G'GATC_C Cuts at: 0 416 726 Size: 416 310 BanI G'GyrC_C Cuts at: 0 104 726 Size: 104 622 BanII G_rGCy'C Cuts at: 0 99 182 263 660 726 Size: 99 83 81 397 66 Fragments arranged by size: 397 99 83 81 66 BbeI G_GCGC'C Cuts at: 0 108 726 Size: 108 618 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 247 281 726 Size: 247 34 445 Fragments arranged by size: 445 247 34 Bme1580I G_kGCm'C Cuts at: 0 99 182 638 660 726 Size: 99 83 456 22 66 Fragments arranged by size: 456 99 83 66 22 BmgBI CAC'GTC Cuts at: 0 315 726 Size: 315 411 BmrI ACTGGGnnnn_n' Cuts at: 0 465 726 Size: 465 261 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 9 318 726 Size: 9 309 408 Fragments arranged by size: 408 309 9 BsaI GGTCTCn'nnnn_ Cuts at: 0 358 726 Size: 358 368 BsaAI yAC'GTr Cuts at: 0 276 633 726 Size: 276 357 93 Fragments arranged by size: 357 276 93 BsaHI Gr'CG_yC Cuts at: 0 49 105 726 Size: 49 56 621 Fragments arranged by size: 621 56 49 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 208 238 316 346 726 Size: 208 30 78 30 380 Fragments arranged by size: 380 208 78 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 10 150 376 422 726 Size: 10 140 226 46 304 Fragments arranged by size: 304 226 140 46 10 BsiHKAI G_wGCw'C Cuts at: 0 263 374 489 638 726 Size: 263 111 115 149 88 Fragments arranged by size: 263 149 115 111 88 BsmBI CGTCTCn'nnnn_ Cuts at: 0 56 726 Size: 56 670 Bsp1286I G_dGCh'C Cuts at: 0 99 182 263 374 489 638 660 726 Size: 99 83 81 111 115 149 22 66 Fragments arranged by size: 149 115 111 99 83 81 66 22 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 591 726 Size: 591 135 BsrFI r'CCGG_y Cuts at: 0 187 726 Size: 187 539 BstEII G'GTnAC_C Cuts at: 0 553 625 726 Size: 553 72 101 Fragments arranged by size: 553 101 72 BstYI r'GATC_y Cuts at: 0 416 726 Size: 416 310 BtgI C'CryG_G Cuts at: 0 287 476 726 Size: 287 189 250 Fragments arranged by size: 287 250 189 EaeI y'GGCC_r Cuts at: 0 146 726 Size: 146 580 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 409 691 726 Size: 409 282 35 Fragments arranged by size: 409 282 35 EcoICRI GAG'CTC Cuts at: 0 261 726 Size: 261 465 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 9 318 726 Size: 9 309 408 Fragments arranged by size: 408 309 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 12 726 Size: 12 714 EcoO109I rG'GnC_Cy Cuts at: 0 96 168 656 657 726 Size: 96 72 488 1 69 Fragments arranged by size: 488 96 72 69 1 HaeII r_GCGC'y Cuts at: 0 108 726 Size: 108 618 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 401 433 726 Size: 401 32 293 Fragments arranged by size: 401 293 32 KasI G'GCGC_C Cuts at: 0 104 726 Size: 104 622 MspA1I CmG'CkG Cuts at: 0 87 726 Size: 87 639 NaeI GCC'GGC Cuts at: 0 189 726 Size: 189 537 NarI GG'CG_CC Cuts at: 0 105 726 Size: 105 621 NcoI C'CATG_G Cuts at: 0 287 476 726 Size: 287 189 250 Fragments arranged by size: 287 250 189 NgoMIV G'CCGG_C Cuts at: 0 187 726 Size: 187 539 NspI r_CATG'y Cuts at: 0 475 726 Size: 475 251 PfoI T'CCnGG_A Cuts at: 0 575 726 Size: 575 151 PmlI CAC'GTG Cuts at: 0 633 726 Size: 633 93 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 316 348 726 Size: 316 32 378 Fragments arranged by size: 378 316 32 PpuMI rG'GwC_Cy Cuts at: 0 168 726 Size: 168 558 PspOMI G'GGCC_C Cuts at: 0 95 178 656 726 Size: 95 83 478 70 Fragments arranged by size: 478 95 83 70 PspXI vC'TCGA_Gb Cuts at: 0 256 726 Size: 256 470 PstI C_TGCA'G Cuts at: 0 398 428 726 Size: 398 30 298 Fragments arranged by size: 398 298 30 SacI G_AGCT'C Cuts at: 0 263 726 Size: 263 463 SanDI GG'GwC_CC Cuts at: 0 168 726 Size: 168 558 SbfI CC_TGCA'GG Cuts at: 0 428 726 Size: 428 298 SfcI C'TryA_G Cuts at: 0 394 424 726 Size: 394 30 302 Fragments arranged by size: 394 302 30 SfoI GGC'GCC Cuts at: 0 106 726 Size: 106 620 SmaI CCC'GGG Cuts at: 0 101 166 307 358 726 Size: 101 65 141 51 368 Fragments arranged by size: 368 141 101 65 51 SmlI C'TyrA_G Cuts at: 0 256 726 Size: 256 470 SphI G_CATG'C Cuts at: 0 475 726 Size: 475 251 StyI C'CwwG_G Cuts at: 0 110 287 476 702 726 Size: 110 177 189 226 24 Fragments arranged by size: 226 189 177 110 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 382 726 Size: 382 344 TatI w'GTAC_w Cuts at: 0 278 726 Size: 278 448 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 208 240 726 Size: 208 32 486 Fragments arranged by size: 486 208 32 XhoI C'TCGA_G Cuts at: 0 256 726 Size: 256 470 XmaI C'CCGG_G Cuts at: 0 99 164 305 356 726 Size: 99 65 141 51 370 Fragments arranged by size: 370 141 99 65 51 XmnI GAAnn'nnTTC Cuts at: 0 328 726 Size: 328 398 Enzymes that do cut: AarI AflIII AloI ApaI ApaLI AvaI BamHI BanI BanII BbeI BcgI Bme1580I BmgBI BmrI BpmI BsaI BsaAI BsaHI BsaXI BseRI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BstEII BstYI BtgI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MspA1I NaeI NarI NcoI NgoMIV NspI PfoI PmlI PpiI PpuMI PspOMI PspXI PstI SacI SanDI SbfI SfcI SfoI SmaI SmlI SphI StyI TaqII TatI TstI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI BmtI BplI Bpu10I BpuEI BsaBI BsaWI BseYI BsgI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EarI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PshAI PsiI PsrI PvuI PvuII RsrII SacII SalI SapI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XcmI ZraI