################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HincII HindII HindIII HpaI HphI KpnI Ksp632I KspI MaeII MaeIII MamI MboI MflI MluI MluNI MroI MscI MslI NaeI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoII XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 99 CGCG CG^CG AcyI 53 GGCGCC G[GA]^CG[CT]C AluI 170 AGCT AG^CT BanI 53 GGCGCC G^G[CT][GA]CC BanII 238 GAGCCC G[GA]GC[CT]^C BbeI 53 GGCGCC GGCGC^C BbsI 246 GTCTTC GAAGAC(2/6) BfaI 5 CTAG C^TAG BmyI 238 GAGCCC G[AGT]GC[ACT]^C BpuAI 246 GTCTTC GAAGAC(2/6) BsaHI 53 GGCGCC G[GA]^CG[CT]C BseRI 148 GAGGAG GAGGAG(10/8) BsgI 94 CTGCAC GTGCAG(16/14) BsiYI 44 CCCTACTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 44 CCCTACTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 40 GTCCC GGGAC(10/14) Bsp1286I 238 GAGCCC G[AGT]GC[ACT]^C BspMI 279 ACCTGC ACCTGC(4/8) BsrBI 117 CCGCTC CCGCTC(-3/-3) BsrI 525 ACTGG ACTGG(1/-1) BstNI 74 CCTGG CC^[AT]GG BstUI 99 CGCG CG^CG BstXI 480 CCAAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EcoRII 74 CCTGG ^CC[AT]GG EcoT14I 384 CCAAGG C^C[AT][AT]GG HaeII 53 GGCGCC [GA]GCGC^[CT] HaeIII 426 GGCC GG^CC Hin1I 53 GGCGCC G[GA]^CG[CT]C KasI 53 GGCGCC G^GCGCC MaeI 5 CTAG C^TAG MboII 247 TCTTC GAAGA(8/7) MfeI 182 CAATTG C^AATTG MseI 416 TTAA T^TAA MspA1I 251 CCGCTG C[AC]G^C[GT]G MunI 182 CAATTG C^AATTG MvaI 74 CCTGG CC^[AT]GG MvnI 99 CGCG CG^CG NarI 53 GGCGCC GG^CGCC PaeR7I 122 CTCGAG C^TCGAG PleI 389 GACTC GAGTC(4/5) PstI 254 CTGCAG CTGCA^G SfcI 254 CTGCAG C^T[GA][CT]AG SfoI 53 GGCGCC GGC^GCC SmaI 264 CCCGGG CCC^GGG SmlI 122 CTCGAG C^T[CT][GA]AG StyI 384 CCAAGG C^C[AT][AT]GG TaqI 123 TCGA T^CGA TfiI 453 GAATC G^A[AT]TC Tru9I 416 TTAA T^TAA TthHB8I 123 TCGA T^CGA XhoI 122 CTCGAG C^TCGAG XmaCI 264 CCCGGG C^CCGGG XmaI 264 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AvaI 122 CTCGAG C^[CT]CG[GA]G 264 CCCGGG BpmI 75 CTGGAG CTGGAG(16/14) 191 CTGGAG BsoBI 122 CTCGAG C^[CT]CG[GA]G 264 CCCGGG BstF5I 381 CATCC GGATG(2/0) 210 GGATG CfoI 54 GCGC GCG^C 100 GCGC FokI 381 CATCC GGATG(9/13) 210 GGATG HgaI 67 GACGC GACGC(5/10) 38 GCGTC HhaI 54 GCGC GCG^C 100 GCGC HinP1I 54 GCGC G^CGC 100 GCGC HinfI 389 GACTC G^A[ACGT]TC 453 GAATC MwoI 56 GCCTCCTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 110 GCTACTTCCGC ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AciI BbvI BcnI BsaJI BsmAI BssKI Cac8I DdeI Fnu4HI HapII HpaII ItaI MnlI MspI NciI NlaIV ScrFI TseI Tsp509I TspRI