################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AfaI AflII AflIII AgeI AhdI AlwNI AocI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraII DrdI Eam1105I EarI Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HgaI HincII HindII HpaI KpnI Ksp632I MaeII MamI MfeI MluI MluNI MroI MscI MunI NaeI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcyI 97 GGCGCC G[GA]^CG[CT]C Alw44I 174 GTGCAC G^TGCAC Aor51HI 23 AGCGCT AGC^GCT ApaLI 174 GTGCAC G^TGCAC BbeI 97 GGCGCC GGCGC^C BbsI 388 GAAGAC GAAGAC(2/6) BcgI 56 GCAGGCTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 407 CTAG C^TAG BpmI 181 CTGGAG CTGGAG(16/14) BpuAI 388 GAAGAC GAAGAC(2/6) BsaHI 97 GGCGCC G[GA]^CG[CT]C BsgI 54 GTGCAG GTGCAG(16/14) BsmFI 310 GGGAC GGGAC(10/14) BspDI 484 ATCGAT AT^CGAT BspMI 609 GCAGGT ACCTGC(4/8) BsrDI 332 GCAATG GCAATG(2/0) BssSI 659 CTCGTG CACGAG(-5/-1) BstAPI 167 GCACCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC ClaI 484 ATCGAT AT^CGAT DraIII 168 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 4 CCGCGG C^C[GA][CT]GG Eco47III 23 AGCGCT AGC^GCT FauI 40 GCGGG CCCGC(4/6) Hin1I 97 GGCGCC G[GA]^CG[CT]C HindIII 627 AAGCTT A^AGCTT KasI 97 GGCGCC G^GCGCC KspI 4 CCGCGG CCGC^GG MaeI 407 CTAG C^TAG MaeIII 206 GTCAC ^GT[ACGT]AC MseI 508 TTAA T^TAA MslI 208 CACTATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 4 CCGCGG C[AC]G^C[GT]G NarI 97 GGCGCC GG^CGCC SacII 4 CCGCGG CCGC^GG SfoI 97 GGCGCC GGC^GCC SmlI 518 CTCAAG C^T[CT][GA]AG TfiI 442 GAATC G^A[AT]TC Tru9I 508 TTAA T^TAA Tsp45I 206 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 319 AAATTT [GA]^AATT[CT] 337 GAATTT ApoI 319 AAATTT [GA]^AATT[CT] 337 GAATTT AspHI 174 GTGCAC G[AT]GC[AT]^C 425 GTGCTC AvaII 14 GGTCC G^G[AT]CC 88 GGACC BcnI 10 CCGGG CC^[GC]GG 91 CCCGG BsiEI 7 CGGCCG CG[GA][CT]^CG 32 CGGCCG BsiHKAI 174 GTGCAC G[AT]GC[AT]^C 425 GTGCTC BsrI 78 ACTGG ACTGG(1/-1) 199 ACTGG BstYI 234 AGATCC [GA]^GATC[CT] 540 GGATCT EaeI 7 CGGCCG [CT]^GGCC[GA] 32 CGGCCG EagI 7 CGGCCG C^GGCCG 32 CGGCCG EclXI 7 CGGCCG C^GGCCG 32 CGGCCG Eco52I 7 CGGCCG C^GGCCG 32 CGGCCG HaeII 23 AGCGCT [GA]GCGC^[CT] 97 GGCGCC HapII 10 CCGG C^CGG 92 CCGG HpaII 10 CCGG C^CGG 92 CCGG HphI 192 GGTGA GGTGA(8/7) 110 TCACC MflI 234 AGATCC [GA]^GATC[CT] 540 GGATCT MspI 10 CCGG C^CGG 92 CCGG NciI 10 CCGGG CC^[GC]GG 91 CCCGG PleI 495 GACTC GAGTC(4/5) 419 GAGTC XcmI 170 CCAAGTGCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 189 CCAGGTGATTACTGG XhoII 234 AGATCC [GA]^GATC[CT] 540 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AccII AciI AluI AlwI BanI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HhaI HinP1I HinfI ItaI MboI MboII MnlI MvaI MvnI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI TaqI TseI Tsp509I TspRI TthHB8I