(Circular) (Six-base) MAPSORT of: pFN21AE2592.seq Check: 8694 from: 1 to: 5377 pFN21AE2592 5377 bp With 182 enzymes: SgfI * October 13, 2015 18:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3187 278 Size: 53 83 186 936 1651 2468 Fragments arranged by size: 2468 1651 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2720 1937 Size: 783 4594 Acc65I G'GTAC_C Cuts at: 2698 2698 Size: 5377 AclI AA'CG_TT Cuts at: 1563 3504 3877 1563 Size: 1941 373 3063 Fragments arranged by size: 3063 1941 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2317 2498 3440 4824 5092 1632 Size: 685 181 942 1384 268 1917 Fragments arranged by size: 1917 1384 942 685 268 181 AfeI AGC'GCT Cuts at: 2030 2030 Size: 5377 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5180 AflIII A'CryG_T Cuts at: 1203 4276 5253 1203 Size: 3073 977 1327 Fragments arranged by size: 3073 1327 977 AgeI A'CCGG_T Cuts at: 4208 4208 Size: 5377 AhdI GACnn_n'nnGTC Cuts at: 4106 4106 Size: 5377 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5377 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5377 AlwNI CAG_nnn'CTG Cuts at: 3120 4692 3120 Size: 1572 3805 ApaLI G'TGCA_C Cuts at: 2179 3434 4590 2179 Size: 1255 1156 2966 Fragments arranged by size: 2966 1255 1156 ApoI r'AATT_y Cuts at: 1493 2324 2342 2686 2912 3064 5173 1493 Size: 831 18 344 226 152 2109 1697 Fragments arranged by size: 2109 1697 831 344 226 152 18 AseI AT'TA_AT Cuts at: 160 1032 3931 160 Size: 872 2899 1606 Fragments arranged by size: 2899 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5377 AvaI C'yCGr_G Cuts at: 1870 1943 2702 1870 Size: 73 759 4545 Fragments arranged by size: 4545 759 73 BamHI G'GATC_C Cuts at: 2707 3074 2707 Size: 367 5010 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2086 2102 2171 2698 4153 Size: 325 217 627 299 16 69 527 1455 Cuts at: 4153 618 Size: 1842 Fragments arranged by size: 1842 1455 627 527 325 299 217 69 16 BanII G_rGCy'C Cuts at: 1391 2696 1391 Size: 1305 4072 BbeI G_GCGC'C Cuts at: 2106 2106 Size: 5377 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2400 928 Size: 971 501 3905 Fragments arranged by size: 3905 971 501 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2050 2084 3568 3602 1903 Size: 34 50 34 29 34 1484 34 3678 Fragments arranged by size: 3678 1484 50 34 34 34 34 29 BciVI GTATCCnnnnn_n' Cuts at: 3269 4479 3269 Size: 1210 4167 BclI T'GATC_A Cuts at: 1548 1548 Size: 5377 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3988 136 Size: 107 122 71 631 605 2316 1525 Fragments arranged by size: 2316 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5372 5372 Size: 5377 BlpI GC'TnA_GC Cuts at: 2794 2794 Size: 5377 Bme1580I G_kGCm'C Cuts at: 1790 2174 2183 3438 4594 1790 Size: 384 9 1255 1156 2573 Fragments arranged by size: 2573 1255 1156 384 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5377 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3108 4066 442 Size: 416 53 83 399 24 1691 958 1753 Fragments arranged by size: 1753 1691 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5377 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2603 2603 Size: 5377 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 4037 1239 Size: 968 1830 2579 Fragments arranged by size: 2579 1830 968 Bpu10I CC'TnA_GC Cuts at: 2166 2166 Size: 5377 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2508 3502 4367 4665 2508 Size: 994 865 298 3220 Fragments arranged by size: 3220 994 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4040 882 Size: 617 2541 2219 Fragments arranged by size: 2541 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3168 493 Size: 711 1964 2702 Fragments arranged by size: 2702 1964 711 BsaBI GATnn'nnATC Cuts at: 5371 5371 Size: 5377 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 3184 3566 4201 Size: 53 83 186 936 570 1081 382 635 Cuts at: 4201 275 Size: 1451 Fragments arranged by size: 1451 1081 936 635 570 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3810 4208 4482 4629 5040 3810 Size: 398 274 147 411 4147 Fragments arranged by size: 4147 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5152 Fragments arranged by size: 5152 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5377 BseYI C'CCAG_C Cuts at: 2031 4580 5076 2031 Size: 2549 496 2332 Fragments arranged by size: 2549 2332 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2080 1163 Size: 573 344 4460 Fragments arranged by size: 4460 573 344 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2015 2040 2827 3588 4616 1540 Size: 275 186 14 25 787 761 1028 2301 Fragments arranged by size: 2301 1028 787 761 275 186 25 14 BsiHKAI G_wGCw'C Cuts at: 2183 2434 3438 3523 4594 2183 Size: 251 1004 85 1071 2966 Fragments arranged by size: 2966 1071 1004 251 85 BsmI GAATG_Cn' Cuts at: 2888 2981 2888 Size: 93 5284 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2174 2183 2434 2696 3438 3523 4594 Size: 399 384 9 251 262 742 85 1071 Cuts at: 4594 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 384 262 251 85 9 BspEI T'CCGG_A Cuts at: 5040 5040 Size: 5377 BspHI T'CATG_A Cuts at: 3266 4996 3266 Size: 1730 3647 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2605 2732 844 Size: 1761 127 3489 Fragments arranged by size: 3489 1761 127 BsrBI CCG'CTC Cuts at: 1394 1823 3264 1394 Size: 429 1441 3507 Fragments arranged by size: 3507 1441 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2344 3872 4046 65 Size: 1183 1096 1528 174 1396 Fragments arranged by size: 1528 1396 1183 1096 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4021 4208 1700 Size: 110 2211 187 2869 Fragments arranged by size: 2869 2211 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5377 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5377 BssSI C'ACGA_G Cuts at: 2664 3437 4449 2664 Size: 773 1012 3592 Fragments arranged by size: 3592 1012 773 BstAPI GCAn_nnn'nTGC Cuts at: 794 2178 794 Size: 1384 3993 BstBI TT'CG_AA Cuts at: 4181 4181 Size: 5377 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5377 BstYI r'GATC_y Cuts at: 1972 2239 2545 2707 3074 3459 3476 4928 5014 Size: 267 306 162 367 385 17 1452 86 Cuts at: 5014 5026 5372 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 306 267 162 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2009 513 Size: 552 944 3881 Fragments arranged by size: 3881 944 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2146 487 Size: 683 56 920 3718 Fragments arranged by size: 3718 920 683 56 BtsI GCAGTG_nn' Cuts at: 2072 2150 2893 3687 3707 2072 Size: 78 743 794 20 3742 Fragments arranged by size: 3742 794 743 78 20 ClaI AT'CG_AT Cuts at: 1554 2490 1554 Size: 936 4441 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5342 DraI TTT'AAA Cuts at: 2681 2681 Size: 5377 DraIII CAC_nnn'GTG Cuts at: 2178 2178 Size: 5377 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4384 809 Size: 507 3068 1802 Fragments arranged by size: 3068 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2012 2037 2824 Size: 54 1425 50 275 18 182 25 787 Cuts at: 2824 3713 5342 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 787 275 182 54 50 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 2012 2037 2824 1537 Size: 275 200 25 787 4090 Fragments arranged by size: 4090 787 275 200 25 EarI CTCTTCn'nnn_ Cuts at: 1353 3307 1353 Size: 1954 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3964 4338 4484 387 Size: 1064 138 2375 374 146 1280 Fragments arranged by size: 2375 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2317 2498 3440 4037 4824 5092 Size: 393 575 110 181 942 597 787 268 Cuts at: 5092 1239 Size: 1524 Fragments arranged by size: 1524 942 787 597 575 393 268 181 110 EcoRI G'AATT_C Cuts at: 2686 3064 2686 Size: 378 4999 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2164 2164 Size: 5377 FspI TGC'GCA Cuts at: 3883 3883 Size: 5377 HaeII r_GCGC'y Cuts at: 1678 2032 2106 4524 1678 Size: 354 74 2418 2531 Fragments arranged by size: 2531 2418 354 74 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4018 4050 4092 4124 1373 Size: 32 135 32 2446 32 42 32 2626 Fragments arranged by size: 2626 2446 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2721 2967 669 Size: 988 281 783 246 3079 Fragments arranged by size: 3079 988 783 281 246 HindIII A'AGCT_T Cuts at: 748 2632 748 Size: 1884 3493 HpaI GTT'AAC Cuts at: 2967 2967 Size: 5377 KasI G'GCGC_C Cuts at: 2102 2102 Size: 5377 KpnI G_GTAC'C Cuts at: 2702 2702 Size: 5377 MfeI C'AATT_G Cuts at: 2976 2976 Size: 5377 MluI A'CGCG_T Cuts at: 5253 5253 Size: 5377 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2368 2650 4491 4675 1305 Size: 1063 282 1841 184 2007 Fragments arranged by size: 2007 1841 1063 282 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3555 Fragments arranged by size: 3555 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2217 3335 3694 3853 518 Size: 648 1051 1118 359 159 2042 Fragments arranged by size: 2042 1118 1051 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2011 2793 3470 4618 4863 1606 Size: 405 782 677 1148 245 2120 Fragments arranged by size: 2120 1148 782 677 405 245 NarI GG'CG_CC Cuts at: 2103 2103 Size: 5377 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4825 NdeI CA'TA_TG Cuts at: 387 387 Size: 5377 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5377 NotI GC'GGCC_GC Cuts at: 2824 2824 Size: 5377 NspI r_CATG'y Cuts at: 1132 1229 2735 4280 1132 Size: 97 1506 1545 2229 Fragments arranged by size: 2229 1545 1506 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5377 PciI A'CATG_T Cuts at: 4276 4276 Size: 5377 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5377 PmeI GTTT'AAAC Cuts at: 2681 2681 Size: 5377 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3446 3478 4959 4991 3446 Size: 32 1481 32 3832 Fragments arranged by size: 3832 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5377 PsiI TTA'TAA Cuts at: 2947 2947 Size: 5377 PstI C_TGCA'G Cuts at: 830 2729 830 Size: 1899 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5377 SacII CC_GC'GG Cuts at: 2012 2012 Size: 5377 SalI G'TCGA_C Cuts at: 1936 2719 1936 Size: 783 4594 SbfI CC_TGCA'GG Cuts at: 2729 2729 Size: 5377 ScaI AGT'ACT Cuts at: 3625 5337 3625 Size: 1712 3665 SfcI C'TryA_G Cuts at: 826 1045 2725 3860 4541 4732 826 Size: 219 1680 1135 681 191 1471 Fragments arranged by size: 1680 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2104 2104 Size: 5377 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5377 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5377 SmaI CCC'GGG Cuts at: 1872 2704 1872 Size: 832 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2523 3481 4382 4644 820 Size: 197 926 580 958 901 262 1553 Fragments arranged by size: 1553 958 926 901 580 262 197 SnaBI TAC'GTA Cuts at: 493 3168 493 Size: 2675 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5377 SphI G_CATG'C Cuts at: 1132 2735 1132 Size: 1603 3774 SspI AAT'ATT Cuts at: 5 52 3301 5 Size: 47 3249 2081 Fragments arranged by size: 3249 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5377 StyI C'CwwG_G Cuts at: 513 1065 2816 513 Size: 552 1751 3074 Fragments arranged by size: 3074 1751 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3404 3421 3574 3759 4204 325 Size: 1255 1824 17 153 185 445 1498 Fragments arranged by size: 1824 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3623 5335 96 Size: 274 80 33 51 1443 1646 1712 138 Fragments arranged by size: 1712 1646 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5337 5369 5337 Size: 32 5345 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5377 XbaI T'CTAG_A Cuts at: 2713 2713 Size: 5377 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2182 2201 2182 Size: 19 5358 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5377 XmaI C'CCGG_G Cuts at: 1870 2702 1870 Size: 832 4545 XmnI GAAnn'nnTTC Cuts at: 3506 3506 Size: 5377 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3185 276 Size: 53 83 186 936 1651 2468 Fragments arranged by size: 2468 1651 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI