Restriction map for
pFN21AE2592
(Linear) (Six-base) MAPSORT of: KIEE2592.seq Check: 5157 from: 1 to: 672
KIEE2592.orf
With 182 enzymes: SgfI *
October 13, 2015 18:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 493 672
Size: 312 181 179
Fragments arranged by size:
312 181 179
AfeI AGC'GCT
Cuts at: 0 25 672
Size: 25 647
ApaLI G'TGCA_C
Cuts at: 0 174 672
Size: 174 498
ApoI r'AATT_y
Cuts at: 0 319 337 672
Size: 319 18 335
Fragments arranged by size:
335 319 18
BanI G'GyrC_C
Cuts at: 0 81 97 166 672
Size: 81 16 69 506
Fragments arranged by size:
506 81 69 16
BbeI G_GCGC'C
Cuts at: 0 101 672
Size: 101 571
BbsI GAAGACnn'nnnn_
Cuts at: 0 395 672
Size: 395 277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 45 79 672
Size: 45 34 593
Fragments arranged by size:
593 45 34
Bme1580I G_kGCm'C
Cuts at: 0 169 178 672
Size: 169 9 494
Fragments arranged by size:
494 169 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 598 672
Size: 598 74
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 672
Size: 202 470
Bpu10I CC'TnA_GC
Cuts at: 0 161 672
Size: 161 511
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 672
Size: 503 169
BsaHI Gr'CG_yC
Cuts at: 0 98 672
Size: 98 574
BseYI C'CCAG_C
Cuts at: 0 26 672
Size: 26 646
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 672
Size: 75 597
BsiEI CG_ry'CG
Cuts at: 0 10 35 672
Size: 10 25 637
Fragments arranged by size:
637 25 10
BsiHKAI G_wGCw'C
Cuts at: 0 178 429 672
Size: 178 251 243
Fragments arranged by size:
251 243 178
Bsp1286I G_dGCh'C
Cuts at: 0 169 178 429 672
Size: 169 9 251 243
Fragments arranged by size:
251 243 169 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 600 672
Size: 600 72
BsrDI GCAATG_nn'
Cuts at: 0 339 672
Size: 339 333
BssSI C'ACGA_G
Cuts at: 0 659 672
Size: 659 13
BstAPI GCAn_nnn'nTGC
Cuts at: 0 173 672
Size: 173 499
BstYI r'GATC_y
Cuts at: 0 234 540 672
Size: 234 306 132
Fragments arranged by size:
306 234 132
BtgI C'CryG_G
Cuts at: 0 4 672
Size: 4 668
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 141 672
Size: 141 531
BtsI GCAGTG_nn'
Cuts at: 0 67 145 672
Size: 67 78 527
Fragments arranged by size:
527 78 67
ClaI AT'CG_AT
Cuts at: 0 485 672
Size: 485 187
DraIII CAC_nnn'GTG
Cuts at: 0 173 672
Size: 173 499
EaeI y'GGCC_r
Cuts at: 0 7 32 672
Size: 7 25 640
Fragments arranged by size:
640 25 7
EagI C'GGCC_G
Cuts at: 0 7 32 672
Size: 7 25 640
Fragments arranged by size:
640 25 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 312 493 672
Size: 202 110 181 179
Fragments arranged by size:
202 181 179 110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 159 672
Size: 159 513
HaeII r_GCGC'y
Cuts at: 0 27 101 672
Size: 27 74 571
Fragments arranged by size:
571 74 27
HindIII A'AGCT_T
Cuts at: 0 627 672
Size: 627 45
KasI G'GCGC_C
Cuts at: 0 97 672
Size: 97 575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 363 645 672
Size: 363 282 27
Fragments arranged by size:
363 282 27
MslI CAynn'nnrTG
Cuts at: 0 212 672
Size: 212 460
MspA1I CmG'CkG
Cuts at: 0 6 672
Size: 6 666
NarI GG'CG_CC
Cuts at: 0 98 672
Size: 98 574
SacII CC_GC'GG
Cuts at: 0 7 672
Size: 7 665
SfoI GGC'GCC
Cuts at: 0 99 672
Size: 99 573
SmlI C'TyrA_G
Cuts at: 0 518 672
Size: 518 154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 177 196 672
Size: 177 19 476
Fragments arranged by size:
476 177 19
Enzymes that do cut:
AcuI AfeI ApaLI ApoI BanI BbeI BbsI BcgI
Bme1580I BplI BpmI Bpu10I BpuEI BsaHI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrDI BssSI BstAPI BstYI
BtgI BtgZI BtsI ClaI DraIII EaeI EagI Eco57MI
FalI HaeII HindIII KasI MmeI MslI MspA1I NarI
SacII SfoI SmlI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbvCI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstBI
BstEII BstXI BstZ17I Bsu36I CspCI DraI DrdI EarI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI Hin4I HincII HpaI KpnI MfeI MluI MscI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI