(Circular) (Six-base) MAPSORT of: pFN21AE2369.seq Check: 8396 from: 1 to: 6445 pFN21AE2369 6445 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4255 278 Size: 53 83 186 936 2719 2468 Fragments arranged by size: 2719 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3788 1937 Size: 1851 4594 Acc65I G'GTAC_C Cuts at: 3009 3100 3766 3009 Size: 91 666 5688 Fragments arranged by size: 5688 666 91 AclI AA'CG_TT Cuts at: 1563 4572 4945 1563 Size: 3009 373 3063 Fragments arranged by size: 3063 3009 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4508 5892 6160 1632 Size: 2876 1384 268 1917 Fragments arranged by size: 2876 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2797 820 Size: 197 1780 4468 Fragments arranged by size: 4468 1780 197 AflIII A'CryG_T Cuts at: 1203 2900 3220 5344 6321 1203 Size: 1697 320 2124 977 1327 Fragments arranged by size: 2124 1697 1327 977 320 AgeI A'CCGG_T Cuts at: 5276 5276 Size: 6445 AhdI GACnn_n'nnGTC Cuts at: 2494 5174 2494 Size: 2680 3765 AleI CACnn'nnGTG Cuts at: 1166 3443 1166 Size: 2277 4168 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6445 AlwNI CAG_nnn'CTG Cuts at: 2104 2162 2942 4188 5760 2104 Size: 58 780 1246 1572 2789 Fragments arranged by size: 2789 1572 1246 780 58 ApaLI G'TGCA_C Cuts at: 3224 4502 5658 3224 Size: 1278 1156 4011 Fragments arranged by size: 4011 1278 1156 ApoI r'AATT_y Cuts at: 1493 3368 3754 3980 4132 6241 1493 Size: 1875 386 226 152 2109 1697 Fragments arranged by size: 2109 1875 1697 386 226 152 AscI GG'CGCG_CC Cuts at: 2380 2380 Size: 6445 AseI AT'TA_AT Cuts at: 160 1032 4999 160 Size: 872 3967 1606 Fragments arranged by size: 3967 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 AvaI C'yCGr_G Cuts at: 1870 1943 2198 2219 3770 1870 Size: 73 255 21 1551 4545 Fragments arranged by size: 4545 1551 255 73 21 BamHI G'GATC_C Cuts at: 3775 4142 3775 Size: 367 6078 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2324 2480 3009 3100 3766 Size: 325 217 627 537 156 529 91 666 Cuts at: 3766 5221 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 666 627 537 529 325 217 156 91 BanII G_rGCy'C Cuts at: 1391 3588 3764 1391 Size: 2197 176 4072 Fragments arranged by size: 4072 2197 176 BbeI G_GCGC'C Cuts at: 2484 2484 Size: 6445 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2921 3516 928 Size: 971 1022 595 3857 Fragments arranged by size: 3857 1022 971 595 BbvCI CC'TCA_GC Cuts at: 2191 2191 Size: 6445 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2530 2564 4636 4670 1903 Size: 34 593 34 2072 34 3678 Fragments arranged by size: 3678 2072 593 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3235 4337 5547 3235 Size: 1102 1210 4133 Fragments arranged by size: 4133 1210 1102 BclI T'GATC_A Cuts at: 1548 2559 3016 1548 Size: 1011 457 4977 Fragments arranged by size: 4977 1011 457 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5056 136 Size: 107 122 71 631 605 3384 1525 Fragments arranged by size: 3384 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6440 6440 Size: 6445 BlpI GC'TnA_GC Cuts at: 2045 2408 3672 3862 2045 Size: 363 1264 190 4628 Fragments arranged by size: 4628 1264 363 190 Bme1580I G_kGCm'C Cuts at: 1790 2310 3228 4506 5662 1790 Size: 520 918 1278 1156 2573 Fragments arranged by size: 2573 1278 1156 918 520 BmgBI CAC'GTC Cuts at: 1318 3658 1318 Size: 2340 4105 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4176 5134 442 Size: 416 53 83 399 24 2759 958 1753 Fragments arranged by size: 2759 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6445 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2104 2104 Size: 6445 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2298 2641 5105 1239 Size: 1059 343 2464 2579 Fragments arranged by size: 2579 2464 1059 343 Bpu10I CC'TnA_GC Cuts at: 2191 2509 2191 Size: 318 6127 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2074 4570 5435 5733 2074 Size: 2496 865 298 2786 Fragments arranged by size: 2786 2496 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2456 5108 882 Size: 617 957 2652 2219 Fragments arranged by size: 2652 2219 957 617 BsaAI yAC'GTr Cuts at: 493 1204 3223 4236 493 Size: 711 2019 1013 2702 Fragments arranged by size: 2702 2019 1013 711 BsaBI GATnn'nnATC Cuts at: 6439 6439 Size: 6445 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2031 2481 2495 4252 Size: 53 83 186 936 498 450 14 1757 Cuts at: 4252 4634 5269 275 Size: 382 635 1451 Fragments arranged by size: 1757 1451 936 635 498 450 382 186 83 53 14 BsaWI w'CCGG_w Cuts at: 4878 5276 5550 5697 6108 4878 Size: 398 274 147 411 5215 Fragments arranged by size: 5215 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2216 2246 1178 Size: 30 165 30 813 30 5377 Fragments arranged by size: 5377 813 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2142 2295 2848 1186 Size: 956 153 553 4783 Fragments arranged by size: 4783 956 553 153 BseYI C'CCAG_C Cuts at: 2368 5648 6144 2368 Size: 3280 496 2669 Fragments arranged by size: 3280 2669 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5872 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2042 3895 4656 5684 1540 Size: 275 186 41 1853 761 1028 2301 Fragments arranged by size: 2301 1853 1028 761 275 186 41 BsiHKAI G_wGCw'C Cuts at: 2226 2878 3228 4506 4591 5662 2226 Size: 652 350 1278 85 1071 3009 Fragments arranged by size: 3009 1278 1071 652 350 85 BsmI GAATG_Cn' Cuts at: 3956 4049 3956 Size: 93 6352 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2310 2878 3228 3588 3764 4506 Size: 399 436 84 568 350 360 176 742 Cuts at: 4506 4591 5662 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 568 436 399 360 350 176 85 84 BspEI T'CCGG_A Cuts at: 6108 6108 Size: 6445 BspHI T'CATG_A Cuts at: 3234 4334 6064 3234 Size: 1100 1730 3615 Fragments arranged by size: 3615 1730 1100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2007 3800 844 Size: 1163 1793 3489 Fragments arranged by size: 3489 1793 1163 BsrBI CCG'CTC Cuts at: 1394 1823 2044 2304 3676 4332 1394 Size: 429 221 260 1372 656 3507 Fragments arranged by size: 3507 1372 656 429 260 221 BsrDI GCAATG_nn' Cuts at: 65 1248 4940 5114 65 Size: 1183 3692 174 1396 Fragments arranged by size: 3692 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2258 2376 5089 5276 1700 Size: 110 448 118 2713 187 2869 Fragments arranged by size: 2869 2713 448 187 118 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6445 BssHII G'CGCG_C Cuts at: 1925 2380 1925 Size: 455 5990 BssSI C'ACGA_G Cuts at: 2078 4505 5517 2078 Size: 2427 1012 3006 Fragments arranged by size: 3006 2427 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2104 2162 794 Size: 1310 58 5077 Fragments arranged by size: 5077 1310 58 BstBI TT'CG_AA Cuts at: 2391 5249 2391 Size: 2858 3587 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6445 BstXI CCAn_nnnn'nTGG Cuts at: 2242 2242 Size: 6445 BstYI r'GATC_y Cuts at: 1972 3775 4142 4527 4544 5996 6082 6094 6440 Size: 1803 367 385 17 1452 86 12 346 Cuts at: 6440 1972 Size: 1977 Fragments arranged by size: 1977 1803 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5893 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3065 487 Size: 683 56 1839 3867 Fragments arranged by size: 3867 1839 683 56 BtsI GCAGTG_nn' Cuts at: 2326 3961 4755 4775 2326 Size: 1635 794 20 3996 Fragments arranged by size: 3996 1635 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6445 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6410 DraI TTT'AAA Cuts at: 3749 3749 Size: 6445 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2470 3656 5452 809 Size: 507 1154 1186 1796 1802 Fragments arranged by size: 1802 1796 1186 1154 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2039 3892 4781 Size: 54 1425 50 275 18 209 1853 889 Cuts at: 4781 6410 8 Size: 1629 43 Fragments arranged by size: 1853 1629 1425 889 275 209 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2039 3892 1537 Size: 275 227 1853 4090 Fragments arranged by size: 4090 1853 275 227 EarI CTCTTCn'nnn_ Cuts at: 1353 3515 3715 4375 1353 Size: 2162 200 660 3423 Fragments arranged by size: 3423 2162 660 200 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2280 5032 5406 5552 387 Size: 1064 138 691 2752 374 146 1280 Fragments arranged by size: 2752 1280 1064 691 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2298 2641 4508 5105 5892 6160 1239 Size: 393 666 343 1867 597 787 268 1524 Fragments arranged by size: 1867 1524 787 666 597 393 343 268 EcoNI CCTnn'n_nnAGG Cuts at: 3691 3691 Size: 6445 EcoRI G'AATT_C Cuts at: 3754 4132 3754 Size: 378 6067 EcoRV GAT'ATC Cuts at: 3063 3063 Size: 6445 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2908 2908 Size: 6445 FspI TGC'GCA Cuts at: 4951 4951 Size: 6445 HaeII r_GCGC'y Cuts at: 1678 2249 2484 5592 1678 Size: 571 235 3108 2531 Fragments arranged by size: 3108 2531 571 235 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2480 2512 2843 2875 3229 Size: 32 135 32 908 32 331 32 354 Cuts at: 3229 3261 5086 5118 5160 5192 1373 Size: 32 1825 32 42 32 2626 Fragments arranged by size: 2626 1825 908 354 331 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3789 4035 669 Size: 988 281 1851 246 3079 Fragments arranged by size: 3079 1851 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6445 HpaI GTT'AAC Cuts at: 4035 4035 Size: 6445 KasI G'GCGC_C Cuts at: 2480 2480 Size: 6445 KpnI G_GTAC'C Cuts at: 3013 3104 3770 3013 Size: 91 666 5688 Fragments arranged by size: 5688 666 91 MfeI C'AATT_G Cuts at: 4044 4044 Size: 6445 MluI A'CGCG_T Cuts at: 6321 6321 Size: 6445 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2104 2259 5559 5743 1305 Size: 799 155 3300 184 2007 Fragments arranged by size: 3300 2007 799 184 155 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4623 Fragments arranged by size: 4623 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2713 3443 4403 4762 4921 518 Size: 648 1547 730 960 359 159 2042 Fragments arranged by size: 2042 1547 960 730 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2013 2162 2180 2762 3250 3275 3861 4538 Size: 407 149 18 582 488 25 586 677 Cuts at: 4538 5686 5931 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 586 582 488 407 245 149 25 18 NarI GG'CG_CC Cuts at: 2481 2481 Size: 6445 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5893 NdeI CA'TA_TG Cuts at: 387 387 Size: 6445 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6445 NotI GC'GGCC_GC Cuts at: 2039 3892 2039 Size: 1853 4592 NspI r_CATG'y Cuts at: 1132 1229 2437 3803 5348 1132 Size: 97 1208 1366 1545 2229 Fragments arranged by size: 2229 1545 1366 1208 97 PasI CC'CwG_GG Cuts at: 1348 3581 1348 Size: 2233 4212 PciI A'CATG_T Cuts at: 5344 5344 Size: 6445 PflMI CCAn_nnn'nTGG Cuts at: 1108 2083 1108 Size: 975 5470 PfoI T'CCnGG_A Cuts at: 3692 3692 Size: 6445 PmeI GTTT'AAAC Cuts at: 3749 3749 Size: 6445 PmlI CAC'GTG Cuts at: 3223 3223 Size: 6445 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4514 4546 6027 6059 4514 Size: 32 1481 32 4900 Fragments arranged by size: 4900 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6445 PsiI TTA'TAA Cuts at: 4015 4015 Size: 6445 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2345 2377 2345 Size: 32 6413 PstI C_TGCA'G Cuts at: 830 2018 2109 2158 3797 830 Size: 1188 91 49 1639 3478 Fragments arranged by size: 3478 1639 1188 91 49 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6445 PvuII CAG'CTG Cuts at: 2013 3250 3275 2013 Size: 1237 25 5183 Fragments arranged by size: 5183 1237 25 SalI G'TCGA_C Cuts at: 1936 3787 1936 Size: 1851 4594 SbfI CC_TGCA'GG Cuts at: 3797 3797 Size: 6445 ScaI AGT'ACT Cuts at: 4693 6405 4693 Size: 1712 4733 SfcI C'TryA_G Cuts at: 826 1045 2014 2105 2154 3180 3793 4928 5609 Size: 219 969 91 49 1026 613 1135 681 Cuts at: 5609 5800 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1026 969 681 613 219 191 91 49 SfoI GGC'GCC Cuts at: 2482 2482 Size: 6445 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 SgrAI Cr'CCGG_yG Cuts at: 1700 2376 1700 Size: 676 5769 SmaI CCC'GGG Cuts at: 1872 3772 1872 Size: 1900 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2053 2797 4549 5450 5712 820 Size: 197 926 110 744 1752 901 262 1553 Fragments arranged by size: 1752 1553 926 901 744 262 197 110 SnaBI TAC'GTA Cuts at: 493 4236 493 Size: 3743 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6445 SphI G_CATG'C Cuts at: 1132 3803 1132 Size: 2671 3774 SspI AAT'ATT Cuts at: 5 52 4369 5 Size: 47 4317 2081 Fragments arranged by size: 4317 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6445 StyI C'CwwG_G Cuts at: 513 1065 3043 3884 513 Size: 552 1978 841 3074 Fragments arranged by size: 3074 1978 841 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2480 2543 3140 4472 4489 4642 4827 Size: 1255 900 63 597 1332 17 153 185 Cuts at: 4827 5272 325 Size: 445 1498 Fragments arranged by size: 1498 1332 1255 900 597 445 185 153 63 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2944 3283 4691 Size: 274 80 33 51 1443 967 339 1408 Cuts at: 4691 6403 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1408 967 339 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2216 2248 6405 6437 2216 Size: 32 4157 32 2224 Fragments arranged by size: 4157 2224 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6445 XbaI T'CTAG_A Cuts at: 2861 3781 2861 Size: 920 5525 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2764 3578 2764 Size: 814 5631 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6445 XmaI C'CCGG_G Cuts at: 1870 3770 1870 Size: 1900 4545 XmnI GAAnn'nnTTC Cuts at: 4574 4574 Size: 6445 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4253 276 Size: 53 83 186 936 2719 2468 Fragments arranged by size: 2719 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI